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Gene information
General information
TC004259 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452024.1: 1..4516
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004259
      iBeetle screen iB_00673

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004259 RA
        MGMCWAPDC KHYSTRDKC HFFSFPKSG KERALWKKL 
        LRRDVEPGP GAYVCSCHF RDGRKENGP ELFLHNIAK 
        RAYFQVESP EKKKMKKQG LPSCSSSAE SLSVDIPEE 
        TVPSTMNLE EVPSTSTAV VAAPADIIQ DAPLESMGV 
        QAPLVSHAA LEAEMYFLK KENAELKAK IQYLTVRFC 
        YENVQGNDK LIVLYTGLP NSQIFEALF HLIEKLDIK 
        YYHKWTVQK LTRIDQLFL TLVKLRLNF PQLDLAQRF 
        GVAQSTVSN IILTFVHVI YEILYKQFM TTMPSREKN 
        KSCLPTCFS NFTNCRAVL DCTEIFTVV SRKSMSTQK 
        LTYSTYKHH NTFKGLIGV APNGVITFV SDLYVGSTS 
        DQKVVLNCG IIDMLKTGD MILADKGFL IQNILPPGV 
        TLNIPPFLS NVQFTPEQV KCTENIARA RIHVERAIR 
        RLKCYHILN FLPESLCHY GDIVFKATA ALTNLQFPL 
        IKEVAELFQ DCDD
      CDS sequence
      • >TC004259 RA
        atgggaatg tgttgggct ccagattgc aaacactat 
        agtactcgc gataaatgc cattttttt agttttcct 
        aagtcggga aaagaacga gcactatgg aaaaaattg 
        ttgagaaga gatgtagaa cccggacca ggagcctac 
        gtttgcagc tgccatttt cgggatgga agaaaggaa 
        aatggtcct gaattattt ttgcacaat atcgcaaaa 
        agagcctat tttcaagtg gaatctcca gaaaaaaaa 
        aagatgaaa aaacaagga ctcccttct tgttctagt 
        tccgctgaa tcattaagt gttgatata ccggaagaa 
        acagtacca tcgacaatg aatttagag gaagtgcca 
        tcgacgtct acagccgta gttgcagca ccagcagat 
        ataattcaa gatgctccg ttagaatct atgggtgtg 
        caagcaccc ttggtgtca catgcggct cttgaagca 
        gaaatgtat tttctcaaa aaggaaaat gctgaactt 
        aaagcaaaa atacaatat ctgacagta cgattttgc 
        tatgaaaat gttcaagga aatgacaaa ctcattgtt 
        ttatatacc ggtcttccc aatagccag attttcgaa 
        gcattgttt cacttaatc gaaaagttg gacataaaa 
        tattaccac aaatggaca gtccaaaag ttaactaga 
        attgaccaa ctcttttta accctagta aaattacga 
        ctcaatttc cctcaactt gatttggcg caacgcttt 
        ggggttgcc caaagcaca gtttctaat attatttta 
        acatttgtc catgtaata tatgaaatt ttatataaa 
        cagtttatg acgacaatg ccttcgcgc gaaaaaaat 
        aaatcttgc ttgccaaca tgttttagc aattttact 
        aattgtaga gcagttcta gattgtacc gaaattttc 
        actgtggta tcacgtaag tctatgtca acacagaaa 
        ttaacatat agtacttac aaacatcat aatactttc 
        aaagggcta attggagtt gcacccaat ggtgtcatc 
        acatttgta agtgattta tacgtggga tcaacttct 
        gatcaaaaa gttgtttta aattgtgga ataatcgac 
        atgttaaaa actggagat atgatttta gccgataag 
        ggatttttg atccaaaat attctgcca ccaggggtc 
        actttgaat attccacct tttttatca aatgtccaa 
        tttacacca gagcaagtt aagtgtacc gaaaatatt 
        gcacgtgca agaatacac gtcgaaagg gcaatacgc 
        cgattaaaa tgttatcat attttaaat tttttgcca 
        gagtctttg tgccactat ggagatatt gtttttaaa 
        gcgactgcc gctttaaca aacctccaa tttccatta 
        attaaagag gtagcagaa ttgtttcag gattgtgat 
        gattaa
      mRNA sequence
      • >TC004259 RA
        ttcttttgg tttaaaaaa aaactgtgt ccattttag 
        tggacataa gtaggtttt tacatcaca actatggga 
        atgtgttgg gctccagat tgcaaacac tatagtact 
        cgcgataaa tgccatttt tttagtttt cctaagtcg 
        ggaaaagaa cgagcacta tggaaaaaa ttgttgaga 
        agagatgta gaacccgga ccaggagcc tacgtttgc 
        agctgccat tttcgggat ggaagaaag gaaaatggt 
        cctgaatta tttttgcac aatatcgca aaaagagcc 
        tattttcaa gtggaatct ccagaaaaa aaaaagatg 
        aaaaaacaa ggactccct tcttgttct agttccgct 
        gaatcatta agtgttgat ataccggaa gaaacagta 
        ccatcgaca atgaattta gaggaagtg ccatcgacg 
        tctacagcc gtagttgca gcaccagca gatataatt 
        caagatgct ccgttagaa tctatgggt gtgcaagca 
        cccttggtg tcacatgcg gctcttgaa gcagaaatg 
        tattttctc aaaaaggaa aatgctgaa cttaaagca 
        aaaatacaa tatctgaca gtacgattt tgctatgaa 
        aatgttcaa ggaaatgac aaactcatt gttttatat 
        accggtctt cccaatagc cagattttc gaagcattg 
        tttcactta atcgaaaag ttggacata aaatattac 
        cacaaatgg acagtccaa aagttaact agaattgac 
        caactcttt ttaacccta gtaaaatta cgactcaat 
        ttccctcaa cttgatttg gcgcaacgc tttggggtt 
        gcccaaagc acagtttct aatattatt ttaacattt 
        gtccatgta atatatgaa attttatat aaacagttt 
        atgacgaca atgccttcg cgcgaaaaa aataaatct 
        tgcttgcca acatgtttt agcaatttt actaattgt 
        agagcagtt ctagattgt accgaaatt ttcactgtg 
        gtatcacgt aagtctatg tcaacacag aaattaaca 
        tatagtact tacaaacat cataatact ttcaaaggg 
        ctaattgga gttgcaccc aatggtgtc atcacattt 
        gtaagtgat ttatacgtg ggatcaact tctgatcaa 
        aaagttgtt ttaaattgt ggaataatc gacatgtta 
        aaaactgga gatatgatt ttagccgat aagggattt 
        ttgatccaa aatattctg ccaccaggg gtcactttg 
        aatattcca cctttttta tcaaatgtc caatttaca 
        ccagagcaa gttaagtgt accgaaaat attgcacgt 
        gcaagaata cacgtcgaa agggcaata cgccgatta 
        aaatgttat catatttta aattttttg ccagagtct 
        ttgtgccac tatggagat attgttttt aaagcgact 
        gccgcttta acaaacctc caatttcca ttaattaaa 
        gaggtagca gaattgttt caggattgt gatgattaa 
        aataatgta aaataaaaa ccatctatt tat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY