| General information | |||
| TC004244 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015451620.1: 114761..122125 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004244 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00672 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC004244 RA
MEEQPKERV RCFFDVSIG GLQSGRIVF ELFTDIVPK TCENFRCLC TGEKGIGVN TKKALHFKG VVFHRVVKD FIIQGGDFS NGNGTGGES VYGGTFEDE NFELKHDQP LLLSMANRG KDTNGSQFF ITTQPAPHL DNVHVVFGR VVGGVDVVR QIESLPVDA NSRPLQDAK IVKCGELVR QVKAKKDKK KKKEENENE EESSGDEKK KKKHKKDKK KEKKEKKHK GKTDSEEEG ELKDLHPLA TVTDINPDD IPDVPFNKF LMRGPQEKD KDRKFNKKR KDDFRRDRN RREWDNHSF NRRDRPITT KSGRIIKGR GKFRYRTPS RSRSRSGTP LHWRKEESR VIKLSEFEK LEQERRKRE ERRKIEETQ PDDNEPDPT TNDDKNKEV DYNALDYED NQSEEEETV KKQVPSLVQ YPLPGTFNP NNETDQKNN KDAMINNTK SDVMAMALG VQVKTGEEE AAKNETKQN DDKKRALPE AANKKEEVL RTGRNKFET EKPDNEYKN GNYREANRR RVERRPFED RRPVRRGFV RRDRGRYVP YPSRDRKYR RSPRSRSRR SHNRHSRSR KTPSPDKEK ATRNRSKSS EDEKKAKKH ESNNQKIDD AEEKYKKLL ILRKQMELL EMKKKKEVE QKLLEEKHR KAKEEADML EKAKKAKRE AIEKEKLLK TYKVLQELD KNSSSKAKR ERSYSSSSS SYSDSSSDS SRQSKRRRR SRSRSRRKD KRRSSSRGR SKRRGYTPK RKR
- >TC004244 RA
atggaggag cagcccaaa gaacgcgtc cgctgcttt ttcgatgtt tcgatcggc ggtttgcag tccggacgc attgttttt gagttattt actgatatt gtgccgaaa acatgcgaa aatttcagg tgtttgtgt acgggggaa aagggcatt ggggtgaat acgaagaaa gcgttgcat ttcaagggg gtggttttc catcgagtc gtcaaggat tttatcatc cagggaggg gatttttcc aatgggaat gggactggg ggtgagagt gtctatgga ggaactttt gaagatgaa aattttgaa ctgaaacac gaccaacct cttctccta tcaatggca aatcgagga aaggacaca aacggttct caattcttt attactaca caaccggcc cctcatctc gacaatgta catgttgtc tttggacgt gttgtcgga ggagttgat gttgtacgt cagattgaa tcactacca gttgatgcc aacagtcgg ccacttcaa gacgcgaaa attgtcaag tgtggcgaa cttgtaaga caggtcaaa gcgaaaaag gacaagaag aagaagaag gaagaaaac gaaaacgag gaggaatca tccggcgat gaaaagaaa aaaaagaaa cacaaaaaa gacaaaaag aaggagaag aaagaaaag aaacacaaa ggaaaaacc gattcggaa gaggaaggc gaactgaaa gatcttcac cctttggcc acagtcact gatattaac cctgatgat atcccggac gttccattc aataaattt ctaatgaga ggtccgcaa gaaaaagat aaggaccga aagtttaac aaaaaaaga aaagatgat tttagaaga gatcggaat cgtcgagag tgggataat cacagtttt aaccgaaga gatcgcccc attacgacc aaaagtggc cgaattatc aaaggaagg ggaaaattc agatacaga accccgtcc cgaagccgc tcccgcagc ggcacgccc ctgcattgg cgtaaggaa gagtcccgc gtgataaaa ctctccgaa ttcgaaaaa ctcgaacaa gagcgccga aaacgtgaa gaacgccgc aaaattgaa gaaacccaa cccgacgac aatgaacca gaccccaca accaacgac gacaaaaac aaagaagtc gattacaac gccctcgac tacgaagac aaccaatcc gaggaggaa gaaacggta aaaaaacaa gtcccaagc ctcgtccag tacccactc cctggcact ttcaaccca aataacgaa accgaccag aaaaacaac aaagacgcg atgattaac aacacaaag agcgacgtc atggcgatg gcgttgggc gtccaggtg aagaccggc gaagaggag gcggcgaaa aacgaaacg aagcaaaac gacgacaag aaacgagca cttcccgaa gctgcgaac aagaaagag gaggtttta cggactgga cgtaataaa ttcgagacg gaaaagccc gataatgaa tataaaaac ggtaattat cgtgaagcg aaccgacgg cgggttgaa agacgtccg tttgaagac cggaggccc gttcggaga ggtttcgtc cgacgggat cgcgggcgc tatgttccc tacccgtca cgtgaccgg aaataccgg aggtcgcca aggtccagg tccaggcgc tcgcataat agacactcc aggagtcgc aaaacaccg tctccagat aaagaaaag gcgacgaga aatcgctcg aaatcgtcc gaggatgag aaaaaagca aaaaaacac gaaagcaac aatcagaag attgacgat gctgaagag aagtataag aagttgttg attttgcgg aaacagatg gaattgctg gaaatgaag aagaagaaa gaggtggag caaaaattg ctggaggag aagcatcgg aaggcgaaa gaggaggct gatatgttg gagaaggca aagaaggcg aaacgtgag gcgattgag aaggagaag ttgttgaag acttataaa gtgttgcaa gagttggat aagaatagt tcgagtaaa gcgaaacga gagaggagt tatagttcc agttcgagc agttatagc gacagttca agcgattct tcgaggcag tcgaagaga cgccgcagg tcccgatcg cgttcccgc aggaaggac aagaggcga agcagttca agaggacga tcgaaacgt cgaggctac actccaaaa aggaaaaga taa
- >TC004244 RA
ttgtgttcg cgaaaaatg tttttttgc cgcaaattc cactaaaaa aaacgaaaa atcggtgaa aattcgctt caaattgat tatcgtccg ttcggtgac tcaatcaat cagtgattt ttgattaaa agtaagatg gaggagcag cccaaagaa cgcgtccgc tgctttttc gatgtttcg atcggcggt ttgcagtcc ggacgcatt gtttttgag ttatttact gatattgtg ccgaaaaca tgcgaaaat ttcaggtgt ttgtgtacg ggggaaaag ggcattggg gtgaatacg aagaaagcg ttgcatttc aagggggtg gttttccat cgagtcgtc aaggatttt atcatccag ggaggggat ttttccaat gggaatggg actgggggt gagagtgtc tatggagga acttttgaa gatgaaaat tttgaactg aaacacgac caacctctt ctcctatca atggcaaat cgaggaaag gacacaaac ggttctcaa ttctttatt actacacaa ccggcccct catctcgac aatgtacat gttgtcttt ggacgtgtt gtcggagga gttgatgtt gtacgtcag attgaatca ctaccagtt gatgccaac agtcggcca cttcaagac gcgaaaatt gtcaagtgt ggcgaactt gtaagacag gtcaaagcg aaaaaggac aagaagaag aagaaggaa gaaaacgaa aacgaggag gaatcatcc ggcgatgaa aagaaaaaa aagaaacac aaaaaagac aaaaagaag gagaagaaa gaaaagaaa cacaaagga aaaaccgat tcggaagag gaaggcgaa ctgaaagat cttcaccct ttggccaca gtcactgat attaaccct gatgatatc ccggacgtt ccattcaat aaatttcta atgagaggt ccgcaagaa aaagataag gaccgaaag tttaacaaa aaaagaaaa gatgatttt agaagagat cggaatcgt cgagagtgg gataatcac agttttaac cgaagagat cgccccatt acgaccaaa agtggccga attatcaaa ggaagggga aaattcaga tacagaacc ccgtcccga agccgctcc cgcagcggc acgcccctg cattggcgt aaggaagag tcccgcgtg ataaaactc tccgaattc gaaaaactc gaacaagag cgccgaaaa cgtgaagaa cgccgcaaa attgaagaa acccaaccc gacgacaat gaaccagac cccacaacc aacgacgac aaaaacaaa gaagtcgat tacaacgcc ctcgactac gaagacaac caatccgag gaggaagaa acggtaaaa aaacaagtc ccaagcctc gtccagtac ccactccct ggcactttc aacccaaat aacgaaacc gaccagaaa aacaacaaa gacgcgatg attaacaac acaaagagc gacgtcatg gcgatggcg ttgggcgtc caggtgaag accggcgaa gaggaggcg gcgaaaaac gaaacgaag caaaacgac gacaagaaa cgagcactt cccgaagct gcgaacaag aaagaggag gttttacgg actggacgt aataaattc gagacggaa aagcccgat aatgaatat aaaaacggt aattatcgt gaagcgaac cgacggcgg gttgaaaga cgtccgttt gaagaccgg aggcccgtt cggagaggt ttcgtccga cgggatcgc gggcgctat gttccctac ccgtcacgt gaccggaaa taccggagg tcgccaagg tccaggtcc aggcgctcg cataataga cactccagg agtcgcaaa acaccgtct ccagataaa gaaaaggcg acgagaaat cgctcgaaa tcgtccgag gatgagaaa aaagcaaaa aaacacgaa agcaacaat cagaagatt gacgatgct gaagagaag tataagaag ttgttgatt ttgcggaaa cagatggaa ttgctggaa atgaagaag aagaaagag gtggagcaa aaattgctg gaggagaag catcggaag gcgaaagag gaggctgat atgttggag aaggcaaag aaggcgaaa cgtgaggcg attgagaag gagaagttg ttgaagact tataaagtg ttgcaagag ttggataag aatagttcg agtaaagcg aaacgagag aggagttat agttccagt tcgagcagt tatagcgac agttcaagc gattcttcg aggcagtcg aagagacgc cgcaggtcc cgatcgcgt tcccgcagg aaggacaag aggcgaagc agttcaaga ggacgatcg aaacgtcga ggctacact ccaaaaagg aaaagataa tgttctatc atcattttt taatttgtc ttttttctt cgattagga ttatcaatt actgaaa
