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Gene information
General information
TC004244 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451620.1: 114761..122125
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004244
    iBeetle screen iB_00672

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004244 RA
      MEEQPKERV RCFFDVSIG GLQSGRIVF ELFTDIVPK 
TCENFRCLC TGEKGIGVN TKKALHFKG VVFHRVVKD 
FIIQGGDFS NGNGTGGES VYGGTFEDE NFELKHDQP 
LLLSMANRG KDTNGSQFF ITTQPAPHL DNVHVVFGR 
VVGGVDVVR QIESLPVDA NSRPLQDAK IVKCGELVR 
QVKAKKDKK KKKEENENE EESSGDEKK KKKHKKDKK 
KEKKEKKHK GKTDSEEEG ELKDLHPLA TVTDINPDD 
IPDVPFNKF LMRGPQEKD KDRKFNKKR KDDFRRDRN 
RREWDNHSF NRRDRPITT KSGRIIKGR GKFRYRTPS 
RSRSRSGTP LHWRKEESR VIKLSEFEK LEQERRKRE 
ERRKIEETQ PDDNEPDPT TNDDKNKEV DYNALDYED 
NQSEEEETV KKQVPSLVQ YPLPGTFNP NNETDQKNN 
KDAMINNTK SDVMAMALG VQVKTGEEE AAKNETKQN 
DDKKRALPE AANKKEEVL RTGRNKFET EKPDNEYKN 
GNYREANRR RVERRPFED RRPVRRGFV RRDRGRYVP 
YPSRDRKYR RSPRSRSRR SHNRHSRSR KTPSPDKEK 
ATRNRSKSS EDEKKAKKH ESNNQKIDD AEEKYKKLL 
ILRKQMELL EMKKKKEVE QKLLEEKHR KAKEEADML 
EKAKKAKRE AIEKEKLLK TYKVLQELD KNSSSKAKR 
ERSYSSSSS SYSDSSSDS SRQSKRRRR SRSRSRRKD 
KRRSSSRGR SKRRGYTPK RKR
    CDS sequence
    • >TC004244 RA
      atggaggag cagcccaaa gaacgcgtc cgctgcttt 
ttcgatgtt tcgatcggc ggtttgcag tccggacgc 
attgttttt gagttattt actgatatt gtgccgaaa 
acatgcgaa aatttcagg tgtttgtgt acgggggaa 
aagggcatt ggggtgaat acgaagaaa gcgttgcat 
ttcaagggg gtggttttc catcgagtc gtcaaggat 
tttatcatc cagggaggg gatttttcc aatgggaat 
gggactggg ggtgagagt gtctatgga ggaactttt 
gaagatgaa aattttgaa ctgaaacac gaccaacct 
cttctccta tcaatggca aatcgagga aaggacaca 
aacggttct caattcttt attactaca caaccggcc 
cctcatctc gacaatgta catgttgtc tttggacgt 
gttgtcgga ggagttgat gttgtacgt cagattgaa 
tcactacca gttgatgcc aacagtcgg ccacttcaa 
gacgcgaaa attgtcaag tgtggcgaa cttgtaaga 
caggtcaaa gcgaaaaag gacaagaag aagaagaag 
gaagaaaac gaaaacgag gaggaatca tccggcgat 
gaaaagaaa aaaaagaaa cacaaaaaa gacaaaaag 
aaggagaag aaagaaaag aaacacaaa ggaaaaacc 
gattcggaa gaggaaggc gaactgaaa gatcttcac 
cctttggcc acagtcact gatattaac cctgatgat 
atcccggac gttccattc aataaattt ctaatgaga 
ggtccgcaa gaaaaagat aaggaccga aagtttaac 
aaaaaaaga aaagatgat tttagaaga gatcggaat 
cgtcgagag tgggataat cacagtttt aaccgaaga 
gatcgcccc attacgacc aaaagtggc cgaattatc 
aaaggaagg ggaaaattc agatacaga accccgtcc 
cgaagccgc tcccgcagc ggcacgccc ctgcattgg 
cgtaaggaa gagtcccgc gtgataaaa ctctccgaa 
ttcgaaaaa ctcgaacaa gagcgccga aaacgtgaa 
gaacgccgc aaaattgaa gaaacccaa cccgacgac 
aatgaacca gaccccaca accaacgac gacaaaaac 
aaagaagtc gattacaac gccctcgac tacgaagac 
aaccaatcc gaggaggaa gaaacggta aaaaaacaa 
gtcccaagc ctcgtccag tacccactc cctggcact 
ttcaaccca aataacgaa accgaccag aaaaacaac 
aaagacgcg atgattaac aacacaaag agcgacgtc 
atggcgatg gcgttgggc gtccaggtg aagaccggc 
gaagaggag gcggcgaaa aacgaaacg aagcaaaac 
gacgacaag aaacgagca cttcccgaa gctgcgaac 
aagaaagag gaggtttta cggactgga cgtaataaa 
ttcgagacg gaaaagccc gataatgaa tataaaaac 
ggtaattat cgtgaagcg aaccgacgg cgggttgaa 
agacgtccg tttgaagac cggaggccc gttcggaga 
ggtttcgtc cgacgggat cgcgggcgc tatgttccc 
tacccgtca cgtgaccgg aaataccgg aggtcgcca 
aggtccagg tccaggcgc tcgcataat agacactcc 
aggagtcgc aaaacaccg tctccagat aaagaaaag 
gcgacgaga aatcgctcg aaatcgtcc gaggatgag 
aaaaaagca aaaaaacac gaaagcaac aatcagaag 
attgacgat gctgaagag aagtataag aagttgttg 
attttgcgg aaacagatg gaattgctg gaaatgaag 
aagaagaaa gaggtggag caaaaattg ctggaggag 
aagcatcgg aaggcgaaa gaggaggct gatatgttg 
gagaaggca aagaaggcg aaacgtgag gcgattgag 
aaggagaag ttgttgaag acttataaa gtgttgcaa 
gagttggat aagaatagt tcgagtaaa gcgaaacga 
gagaggagt tatagttcc agttcgagc agttatagc 
gacagttca agcgattct tcgaggcag tcgaagaga 
cgccgcagg tcccgatcg cgttcccgc aggaaggac 
aagaggcga agcagttca agaggacga tcgaaacgt 
cgaggctac actccaaaa aggaaaaga taa
    mRNA sequence
    • >TC004244 RA
      ttgtgttcg cgaaaaatg tttttttgc cgcaaattc 
cactaaaaa aaacgaaaa atcggtgaa aattcgctt 
caaattgat tatcgtccg ttcggtgac tcaatcaat 
cagtgattt ttgattaaa agtaagatg gaggagcag 
cccaaagaa cgcgtccgc tgctttttc gatgtttcg 
atcggcggt ttgcagtcc ggacgcatt gtttttgag 
ttatttact gatattgtg ccgaaaaca tgcgaaaat 
ttcaggtgt ttgtgtacg ggggaaaag ggcattggg 
gtgaatacg aagaaagcg ttgcatttc aagggggtg 
gttttccat cgagtcgtc aaggatttt atcatccag 
ggaggggat ttttccaat gggaatggg actgggggt 
gagagtgtc tatggagga acttttgaa gatgaaaat 
tttgaactg aaacacgac caacctctt ctcctatca 
atggcaaat cgaggaaag gacacaaac ggttctcaa 
ttctttatt actacacaa ccggcccct catctcgac 
aatgtacat gttgtcttt ggacgtgtt gtcggagga 
gttgatgtt gtacgtcag attgaatca ctaccagtt 
gatgccaac agtcggcca cttcaagac gcgaaaatt 
gtcaagtgt ggcgaactt gtaagacag gtcaaagcg 
aaaaaggac aagaagaag aagaaggaa gaaaacgaa 
aacgaggag gaatcatcc ggcgatgaa aagaaaaaa 
aagaaacac aaaaaagac aaaaagaag gagaagaaa 
gaaaagaaa cacaaagga aaaaccgat tcggaagag 
gaaggcgaa ctgaaagat cttcaccct ttggccaca 
gtcactgat attaaccct gatgatatc ccggacgtt 
ccattcaat aaatttcta atgagaggt ccgcaagaa 
aaagataag gaccgaaag tttaacaaa aaaagaaaa 
gatgatttt agaagagat cggaatcgt cgagagtgg 
gataatcac agttttaac cgaagagat cgccccatt 
acgaccaaa agtggccga attatcaaa ggaagggga 
aaattcaga tacagaacc ccgtcccga agccgctcc 
cgcagcggc acgcccctg cattggcgt aaggaagag 
tcccgcgtg ataaaactc tccgaattc gaaaaactc 
gaacaagag cgccgaaaa cgtgaagaa cgccgcaaa 
attgaagaa acccaaccc gacgacaat gaaccagac 
cccacaacc aacgacgac aaaaacaaa gaagtcgat 
tacaacgcc ctcgactac gaagacaac caatccgag 
gaggaagaa acggtaaaa aaacaagtc ccaagcctc 
gtccagtac ccactccct ggcactttc aacccaaat 
aacgaaacc gaccagaaa aacaacaaa gacgcgatg 
attaacaac acaaagagc gacgtcatg gcgatggcg 
ttgggcgtc caggtgaag accggcgaa gaggaggcg 
gcgaaaaac gaaacgaag caaaacgac gacaagaaa 
cgagcactt cccgaagct gcgaacaag aaagaggag 
gttttacgg actggacgt aataaattc gagacggaa 
aagcccgat aatgaatat aaaaacggt aattatcgt 
gaagcgaac cgacggcgg gttgaaaga cgtccgttt 
gaagaccgg aggcccgtt cggagaggt ttcgtccga 
cgggatcgc gggcgctat gttccctac ccgtcacgt 
gaccggaaa taccggagg tcgccaagg tccaggtcc 
aggcgctcg cataataga cactccagg agtcgcaaa 
acaccgtct ccagataaa gaaaaggcg acgagaaat 
cgctcgaaa tcgtccgag gatgagaaa aaagcaaaa 
aaacacgaa agcaacaat cagaagatt gacgatgct 
gaagagaag tataagaag ttgttgatt ttgcggaaa 
cagatggaa ttgctggaa atgaagaag aagaaagag 
gtggagcaa aaattgctg gaggagaag catcggaag 
gcgaaagag gaggctgat atgttggag aaggcaaag 
aaggcgaaa cgtgaggcg attgagaag gagaagttg 
ttgaagact tataaagtg ttgcaagag ttggataag 
aatagttcg agtaaagcg aaacgagag aggagttat 
agttccagt tcgagcagt tatagcgac agttcaagc 
gattcttcg aggcagtcg aagagacgc cgcaggtcc 
cgatcgcgt tcccgcagg aaggacaag aggcgaagc 
agttcaaga ggacgatcg aaacgtcga ggctacact 
ccaaaaagg aaaagataa tgttctatc atcattttt 
taatttgtc ttttttctt cgattagga ttatcaatt 
actgaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY