Status
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Gene information
General information
TC004244 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451620.1: 114761..122125
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004244
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00672

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004244 RA
      MEEQPKERV RCFFDVSIG GLQSGRIVF ELFTDIVPK 
      TCENFRCLC TGEKGIGVN TKKALHFKG VVFHRVVKD 
      FIIQGGDFS NGNGTGGES VYGGTFEDE NFELKHDQP 
      LLLSMANRG KDTNGSQFF ITTQPAPHL DNVHVVFGR 
      VVGGVDVVR QIESLPVDA NSRPLQDAK IVKCGELVR 
      QVKAKKDKK KKKEENENE EESSGDEKK KKKHKKDKK 
      KEKKEKKHK GKTDSEEEG ELKDLHPLA TVTDINPDD 
      IPDVPFNKF LMRGPQEKD KDRKFNKKR KDDFRRDRN 
      RREWDNHSF NRRDRPITT KSGRIIKGR GKFRYRTPS 
      RSRSRSGTP LHWRKEESR VIKLSEFEK LEQERRKRE 
      ERRKIEETQ PDDNEPDPT TNDDKNKEV DYNALDYED 
      NQSEEEETV KKQVPSLVQ YPLPGTFNP NNETDQKNN 
      KDAMINNTK SDVMAMALG VQVKTGEEE AAKNETKQN 
      DDKKRALPE AANKKEEVL RTGRNKFET EKPDNEYKN 
      GNYREANRR RVERRPFED RRPVRRGFV RRDRGRYVP 
      YPSRDRKYR RSPRSRSRR SHNRHSRSR KTPSPDKEK 
      ATRNRSKSS EDEKKAKKH ESNNQKIDD AEEKYKKLL 
      ILRKQMELL EMKKKKEVE QKLLEEKHR KAKEEADML 
      EKAKKAKRE AIEKEKLLK TYKVLQELD KNSSSKAKR 
      ERSYSSSSS SYSDSSSDS SRQSKRRRR SRSRSRRKD 
      KRRSSSRGR SKRRGYTPK RKR
    CDS sequence
    • >TC004244 RA
      atggaggag cagcccaaa gaacgcgtc cgctgcttt 
      ttcgatgtt tcgatcggc ggtttgcag tccggacgc 
      attgttttt gagttattt actgatatt gtgccgaaa 
      acatgcgaa aatttcagg tgtttgtgt acgggggaa 
      aagggcatt ggggtgaat acgaagaaa gcgttgcat 
      ttcaagggg gtggttttc catcgagtc gtcaaggat 
      tttatcatc cagggaggg gatttttcc aatgggaat 
      gggactggg ggtgagagt gtctatgga ggaactttt 
      gaagatgaa aattttgaa ctgaaacac gaccaacct 
      cttctccta tcaatggca aatcgagga aaggacaca 
      aacggttct caattcttt attactaca caaccggcc 
      cctcatctc gacaatgta catgttgtc tttggacgt 
      gttgtcgga ggagttgat gttgtacgt cagattgaa 
      tcactacca gttgatgcc aacagtcgg ccacttcaa 
      gacgcgaaa attgtcaag tgtggcgaa cttgtaaga 
      caggtcaaa gcgaaaaag gacaagaag aagaagaag 
      gaagaaaac gaaaacgag gaggaatca tccggcgat 
      gaaaagaaa aaaaagaaa cacaaaaaa gacaaaaag 
      aaggagaag aaagaaaag aaacacaaa ggaaaaacc 
      gattcggaa gaggaaggc gaactgaaa gatcttcac 
      cctttggcc acagtcact gatattaac cctgatgat 
      atcccggac gttccattc aataaattt ctaatgaga 
      ggtccgcaa gaaaaagat aaggaccga aagtttaac 
      aaaaaaaga aaagatgat tttagaaga gatcggaat 
      cgtcgagag tgggataat cacagtttt aaccgaaga 
      gatcgcccc attacgacc aaaagtggc cgaattatc 
      aaaggaagg ggaaaattc agatacaga accccgtcc 
      cgaagccgc tcccgcagc ggcacgccc ctgcattgg 
      cgtaaggaa gagtcccgc gtgataaaa ctctccgaa 
      ttcgaaaaa ctcgaacaa gagcgccga aaacgtgaa 
      gaacgccgc aaaattgaa gaaacccaa cccgacgac 
      aatgaacca gaccccaca accaacgac gacaaaaac 
      aaagaagtc gattacaac gccctcgac tacgaagac 
      aaccaatcc gaggaggaa gaaacggta aaaaaacaa 
      gtcccaagc ctcgtccag tacccactc cctggcact 
      ttcaaccca aataacgaa accgaccag aaaaacaac 
      aaagacgcg atgattaac aacacaaag agcgacgtc 
      atggcgatg gcgttgggc gtccaggtg aagaccggc 
      gaagaggag gcggcgaaa aacgaaacg aagcaaaac 
      gacgacaag aaacgagca cttcccgaa gctgcgaac 
      aagaaagag gaggtttta cggactgga cgtaataaa 
      ttcgagacg gaaaagccc gataatgaa tataaaaac 
      ggtaattat cgtgaagcg aaccgacgg cgggttgaa 
      agacgtccg tttgaagac cggaggccc gttcggaga 
      ggtttcgtc cgacgggat cgcgggcgc tatgttccc 
      tacccgtca cgtgaccgg aaataccgg aggtcgcca 
      aggtccagg tccaggcgc tcgcataat agacactcc 
      aggagtcgc aaaacaccg tctccagat aaagaaaag 
      gcgacgaga aatcgctcg aaatcgtcc gaggatgag 
      aaaaaagca aaaaaacac gaaagcaac aatcagaag 
      attgacgat gctgaagag aagtataag aagttgttg 
      attttgcgg aaacagatg gaattgctg gaaatgaag 
      aagaagaaa gaggtggag caaaaattg ctggaggag 
      aagcatcgg aaggcgaaa gaggaggct gatatgttg 
      gagaaggca aagaaggcg aaacgtgag gcgattgag 
      aaggagaag ttgttgaag acttataaa gtgttgcaa 
      gagttggat aagaatagt tcgagtaaa gcgaaacga 
      gagaggagt tatagttcc agttcgagc agttatagc 
      gacagttca agcgattct tcgaggcag tcgaagaga 
      cgccgcagg tcccgatcg cgttcccgc aggaaggac 
      aagaggcga agcagttca agaggacga tcgaaacgt 
      cgaggctac actccaaaa aggaaaaga taa
    mRNA sequence
    • >TC004244 RA
      ttgtgttcg cgaaaaatg tttttttgc cgcaaattc 
      cactaaaaa aaacgaaaa atcggtgaa aattcgctt 
      caaattgat tatcgtccg ttcggtgac tcaatcaat 
      cagtgattt ttgattaaa agtaagatg gaggagcag 
      cccaaagaa cgcgtccgc tgctttttc gatgtttcg 
      atcggcggt ttgcagtcc ggacgcatt gtttttgag 
      ttatttact gatattgtg ccgaaaaca tgcgaaaat 
      ttcaggtgt ttgtgtacg ggggaaaag ggcattggg 
      gtgaatacg aagaaagcg ttgcatttc aagggggtg 
      gttttccat cgagtcgtc aaggatttt atcatccag 
      ggaggggat ttttccaat gggaatggg actgggggt 
      gagagtgtc tatggagga acttttgaa gatgaaaat 
      tttgaactg aaacacgac caacctctt ctcctatca 
      atggcaaat cgaggaaag gacacaaac ggttctcaa 
      ttctttatt actacacaa ccggcccct catctcgac 
      aatgtacat gttgtcttt ggacgtgtt gtcggagga 
      gttgatgtt gtacgtcag attgaatca ctaccagtt 
      gatgccaac agtcggcca cttcaagac gcgaaaatt 
      gtcaagtgt ggcgaactt gtaagacag gtcaaagcg 
      aaaaaggac aagaagaag aagaaggaa gaaaacgaa 
      aacgaggag gaatcatcc ggcgatgaa aagaaaaaa 
      aagaaacac aaaaaagac aaaaagaag gagaagaaa 
      gaaaagaaa cacaaagga aaaaccgat tcggaagag 
      gaaggcgaa ctgaaagat cttcaccct ttggccaca 
      gtcactgat attaaccct gatgatatc ccggacgtt 
      ccattcaat aaatttcta atgagaggt ccgcaagaa 
      aaagataag gaccgaaag tttaacaaa aaaagaaaa 
      gatgatttt agaagagat cggaatcgt cgagagtgg 
      gataatcac agttttaac cgaagagat cgccccatt 
      acgaccaaa agtggccga attatcaaa ggaagggga 
      aaattcaga tacagaacc ccgtcccga agccgctcc 
      cgcagcggc acgcccctg cattggcgt aaggaagag 
      tcccgcgtg ataaaactc tccgaattc gaaaaactc 
      gaacaagag cgccgaaaa cgtgaagaa cgccgcaaa 
      attgaagaa acccaaccc gacgacaat gaaccagac 
      cccacaacc aacgacgac aaaaacaaa gaagtcgat 
      tacaacgcc ctcgactac gaagacaac caatccgag 
      gaggaagaa acggtaaaa aaacaagtc ccaagcctc 
      gtccagtac ccactccct ggcactttc aacccaaat 
      aacgaaacc gaccagaaa aacaacaaa gacgcgatg 
      attaacaac acaaagagc gacgtcatg gcgatggcg 
      ttgggcgtc caggtgaag accggcgaa gaggaggcg 
      gcgaaaaac gaaacgaag caaaacgac gacaagaaa 
      cgagcactt cccgaagct gcgaacaag aaagaggag 
      gttttacgg actggacgt aataaattc gagacggaa 
      aagcccgat aatgaatat aaaaacggt aattatcgt 
      gaagcgaac cgacggcgg gttgaaaga cgtccgttt 
      gaagaccgg aggcccgtt cggagaggt ttcgtccga 
      cgggatcgc gggcgctat gttccctac ccgtcacgt 
      gaccggaaa taccggagg tcgccaagg tccaggtcc 
      aggcgctcg cataataga cactccagg agtcgcaaa 
      acaccgtct ccagataaa gaaaaggcg acgagaaat 
      cgctcgaaa tcgtccgag gatgagaaa aaagcaaaa 
      aaacacgaa agcaacaat cagaagatt gacgatgct 
      gaagagaag tataagaag ttgttgatt ttgcggaaa 
      cagatggaa ttgctggaa atgaagaag aagaaagag 
      gtggagcaa aaattgctg gaggagaag catcggaag 
      gcgaaagag gaggctgat atgttggag aaggcaaag 
      aaggcgaaa cgtgaggcg attgagaag gagaagttg 
      ttgaagact tataaagtg ttgcaagag ttggataag 
      aatagttcg agtaaagcg aaacgagag aggagttat 
      agttccagt tcgagcagt tatagcgac agttcaagc 
      gattcttcg aggcagtcg aagagacgc cgcaggtcc 
      cgatcgcgt tcccgcagg aaggacaag aggcgaagc 
      agttcaaga ggacgatcg aaacgtcga ggctacact 
      ccaaaaagg aaaagataa tgttctatc atcattttt 
      taatttgtc ttttttctt cgattagga ttatcaatt 
      actgaaa
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