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Gene information
General information
TC004159 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 1557575..1560626
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004159
    iBeetle screen iB_00670
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    vitellarium thinner - Number of animals showing the phenotype: 4
    ovary size decreased - Number of animals showing the phenotype: 4
    vitellarium shortened - Number of animals showing the phenotype: 4
    egg chamber not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004159 RA
      MGRWVWSRA ECGSSGDCL EEVSKSAVC ARGPITDDL 
      MCLKMMTFG LAKCTITGV DGHGPRDGG TPILLVAGA 
      ATGHLQANL QIAAAPPGG AREGSCHWS RTNSVNLSV 
      LGADMLALL CLCVVLLVW WFSRPKKSP STIPGPRPW 
      PLIGSMHLL AGHETPFQA FTALSRVYG DIFSIHLGS 
      ASCVVVNNF KLIKEVLIA KGGDFGGRP DFARFHKLF 
      GGDRNNSLA LCDWSSLQK TRRSIARTY CSPRFTSLQ 
      YDRVNNVGE EELKSFLHQ LDQLPHGQP CNVKPAVLM 
      VCANMFTQY MCSTSFAYE DKGFQKIVR YFDEIFWEI 
      NQGYAVDFL PWLLPVYTG HMKKISNWA TEIRQFILS 
      RIIDKHRAT LDTNSPPRD FTDALLMHL EEDPNMNWQ 
      HIIFELEDF LGGHSAIGN LVMVTLAAV VDHPEVAKR 
      IQEEVDQVT GGTRCPNLF DKAAMPYTE ATILETLRT 
      ASSPIVPHV ASKDTEIDG HEVSKGTIV FINNYELNQ 
      GDAYWDEPG LFKPERFLS STGNIVKPA HFIPFSTGK 
      RTCIGQRLV QCFSFVVLA TLLQYYDVS TKESVKVQP 
      GCVAVPPDC FKLVLTPRK
    CDS sequence
    • >TC004159 RA
      atgggccga tgggtttgg tctcgtgca gagtgtgga 
      tcaagtggg gactgtttg gaggaggtc tccaaaagc 
      gcggtctgc gcgcggggt cctattact gatgacttg 
      atgtgcttg aaaatgatg accttcggt ctggcgaag 
      tgtaccatc acgggcgta gacggtcac gggccgcga 
      gacggtggg actcctatt ttgctagtg gcgggggcg 
      gccacaggc catcttcag gccaatctc cagatcgca 
      gctgcaccc cctggtggg gcaagagag gggagctgt 
      cattggtcg cgcaccaac tccgtcaat ctaagtgta 
      ctcggtgca gacatgctc gcgctcctc tgtctgtgt 
      gtggtgctg ctggtgtgg tggttctcg cggccgaag 
      aagtccccg agtacgatt ccggggccg cggccctgg 
      cccctcatc ggcagcatg cacctcctg gccgggcac 
      gagacgccc ttccaggcc ttcaccgcg ctcagtcgg 
      gtctacgga gacatcttc agcatccac ctcgggtcg 
      gcttcctgt gtcgtcgtc aacaacttc aagctgatc 
      aaagaggtg ctcatcgcc aaggggggc gacttcggg 
      ggccggccc gacttcgct cgcttccat aagctcttc 
      ggcggcgat cgcaacaat tcgctcgcc ctgtgtgac 
      tggtcctcg ctgcagaag acgcgccgc agcatcgcc 
      cgcacttac tgctcgccc agattcact tcgctccag 
      tacgacaga gtcaacaac gtcggcgaa gaggagctc 
      aagtccttc ctccaccaa ctggaccaa ctcccccac 
      ggccagccc tgcaacgtc aagccggcc gtcctcatg 
      gtctgcgcc aacatgttc acccagtac atgtgctcc 
      acaagcttc gcctacgag gacaaaggc ttccagaag 
      atcgtccgg tatttcgat gagatcttc tgggagatc 
      aatcaaggc tatgccgtc gacttcctc ccctggctc 
      ctcccggtc tacaccggc cacatgaag aaaatctcc 
      aactgggcc acggaaatc cgacagttt atcttgtcg 
      aggatcatt gacaaacac cgcgcgacc ttagacacc 
      aactcccca ccgagagac ttcaccgat gctcttttg 
      atgcacttg gaggaagac ccaaatatg aattggcaa 
      catatcata ttcgaattg gaagatttc ctcggggga 
      cactcggcc atcggtaac ttggtcatg gtaactcta 
      gccgcagtc gtcgatcat ccggaagtc gcgaaacgt 
      attcaggaa gaagtcgac caagtgact ggcggtact 
      cgctgcccg aaccttttc gacaaagct gcgatgccc 
      tacacggaa gccaccatc ttggaaact ctccgaaca 
      gcctcttcg cccattgtc ccacacgtc gcgagtaaa 
      gacaccgaa atcgacgga cacgaagtc agcaaaggc 
      acgattgta ttcattaac aactacgag ctgaatcaa 
      ggcgatgct tattgggac gagcctgga ctgttcaag 
      cctgaacga ttcctttcg agcaccggc aatatcgtc 
      aaaccggca catttcatt cctttcagc acgggaaag 
      aggacgtgc atcggccaa aggctcgtc caatgtttc 
      agtttcgtg gttttagca acacttctg cagtattat 
      gacgtcagt acaaaagaa agcgtaaag gtacaacct 
      ggctgcgtt gcggtcccg cctgattgt ttcaagcta 
      gtattgaca ccaagaaaa taa
    mRNA sequence
    • >TC004159 RA
      aagtcgaag acggcggtg atgggccga tgggtttgg 
      tctcgtgca gagtgtgga tcaagtggg gactgtttg 
      gaggaggtc tccaaaagc gcggtctgc gcgcggggt 
      cctattact gatgacttg atgtgcttg aaaatgatg 
      accttcggt ctggcgaag tgtaccatc acgggcgta 
      gacggtcac gggccgcga gacggtggg actcctatt 
      ttgctagtg gcgggggcg gccacaggc catcttcag 
      gccaatctc cagatcgca gctgcaccc cctggtggg 
      gcaagagag gggagctgt cattggtcg cgcaccaac 
      tccgtcaat ctaagtgta ctcggtgca gacatgctc 
      gcgctcctc tgtctgtgt gtggtgctg ctggtgtgg 
      tggttctcg cggccgaag aagtccccg agtacgatt 
      ccggggccg cggccctgg cccctcatc ggcagcatg 
      cacctcctg gccgggcac gagacgccc ttccaggcc 
      ttcaccgcg ctcagtcgg gtctacgga gacatcttc 
      agcatccac ctcgggtcg gcttcctgt gtcgtcgtc 
      aacaacttc aagctgatc aaagaggtg ctcatcgcc 
      aaggggggc gacttcggg ggccggccc gacttcgct 
      cgcttccat aagctcttc ggcggcgat cgcaacaat 
      tcgctcgcc ctgtgtgac tggtcctcg ctgcagaag 
      acgcgccgc agcatcgcc cgcacttac tgctcgccc 
      agattcact tcgctccag tacgacaga gtcaacaac 
      gtcggcgaa gaggagctc aagtccttc ctccaccaa 
      ctggaccaa ctcccccac ggccagccc tgcaacgtc 
      aagccggcc gtcctcatg gtctgcgcc aacatgttc 
      acccagtac atgtgctcc acaagcttc gcctacgag 
      gacaaaggc ttccagaag atcgtccgg tatttcgat 
      gagatcttc tgggagatc aatcaaggc tatgccgtc 
      gacttcctc ccctggctc ctcccggtc tacaccggc 
      cacatgaag aaaatctcc aactgggcc acggaaatc 
      cgacagttt atcttgtcg aggatcatt gacaaacac 
      cgcgcgacc ttagacacc aactcccca ccgagagac 
      ttcaccgat gctcttttg atgcacttg gaggaagac 
      ccaaatatg aattggcaa catatcata ttcgaattg 
      gaagatttc ctcggggga cactcggcc atcggtaac 
      ttggtcatg gtaactcta gccgcagtc gtcgatcat 
      ccggaagtc gcgaaacgt attcaggaa gaagtcgac 
      caagtgact ggcggtact cgctgcccg aaccttttc 
      gacaaagct gcgatgccc tacacggaa gccaccatc 
      ttggaaact ctccgaaca gcctcttcg cccattgtc 
      ccacacgtc gcgagtaaa gacaccgaa atcgacgga 
      cacgaagtc agcaaaggc acgattgta ttcattaac 
      aactacgag ctgaatcaa ggcgatgct tattgggac 
      gagcctgga ctgttcaag cctgaacga ttcctttcg 
      agcaccggc aatatcgtc aaaccggca catttcatt 
      cctttcagc acgggaaag aggacgtgc atcggccaa 
      aggctcgtc caatgtttc agtttcgtg gttttagca 
      acacttctg cagtattat gacgtcagt acaaaagaa 
      agcgtaaag gtacaacct ggctgcgtt gcggtcccg 
      cctgattgt ttcaagcta gtattgaca ccaagaaaa 
      taaacgatg tgtagtact gaaccggtg agcgattag 
      gcgatgctt atcggaggg tattttgta agattgcgg 
      ctgtaatca caggtagtt tgtatgaca gtggagagg 
      gcgggcgcc ctgccggaa cttgtacgg ccactaaaa 
      gacgaggct ttaagccaa acatcacct tcatatttt 
      ttcccattt attttatta tcgttattt tatcacgca 
      ttcattgta tatatatca ttatgtgaa tttattgta 
      aagatgttc taataattt atccttaat tgtagtctc 
      ccagtttta agtccaata taatgagac tgaataaag 
      tgtgtattt tatt