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Gene information
General information
TC004151 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1714739..1716895
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004151
    iBeetle screen iB_00669

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004151 RA
      MNPSSGMCE SLITWFQSI VPSRARNIE EICDGVAML 
      EALIQIAPV HFSKLEPKI KRDVSSSWR LRVSNLKKI 
      FEAISEYYQ DVLTLQLLE DGRPDVTEI GANNDPVQL 
      SKLLRLILG CAINCERKQ EYITKIMDM EESVQQNIK 
      QAIEQLDLV TGGRSGSLL ILDSDTRVS QLVGELEVA 
      NEAKETLSQ QCQQMETQL HNLLEENQA LLAENQALK 
      DKESKGSDL RKQNEELKE ELFKAEVMR DDFKAKITE 
      QQKQIKANQ EKIAELQLA ANDTSRLKD EVDALSESA 
      AKVQVLEAN LASYKKKLE KYADAKKTL QKLEEKNME 
      YLQKILQHE EELAKVTSW KSQCETYKK QLMEMEQKL 
      DEETQRADK AQFNCEKLE NKLVALQGE RERIIQERD 
      VLREEMDEL KLGHPKKEA GAAMAQELT PTEMKERLR 
      FLEKENKTL RTSLQEHEA NQALLESAL ARVEKLQQQ 
      KRSANQTVL KLEAQLEEL KNKVASDEP QHADSLVKE 
      YKQKIATLQ EALTVKESE LQTAQAKYT RNLEKAREV 
      AQQLEPKSN GGIDSLNNT RHMKEMEER LMAAAFYKL 
      GMTCHREAI DERLAVLSA GQGQSFLAR QRQPTPRKQ 
      IQRFKSK
    CDS sequence
    • >TC004151 RA
      atgaatcca agtagtgga atgtgtgaa agtttgata 
      acttggttc caaagtata gtgccaagt agagctcga 
      aatattgaa gaaatatgt gatggggtt gcaatgtta 
      gaagcttta atacaaata gcaccggtc cactttagc 
      aaattagaa ccaaaaata aagcgagat gttagttca 
      agttggagg ctgcgtgtc agtaattta aagaaaatt 
      tttgaagct attagtgaa tattatcag gacgtcctc 
      accctccaa ctgcttgaa gatgggcga cctgatgtg 
      actgaaatt ggggccaac aatgatcct gtgcaatta 
      tccaagctt ttacggtta attttagga tgtgcaata 
      aactgcgag agaaagcaa gaatacatc accaaaata 
      atggacatg gaagaatca gtgcaacaa aacataaaa 
      caagcaatt gaacagctc gacttagtg actgggggc 
      cgttctggc agtctttta atcctcgac agtgacacc 
      agagtgtcc cagctagtc ggcgaactc gaggttgca 
      aacgaagct aaagagacc ttgagtcaa cagtgccaa 
      caaatggag acccagctc cacaacctc ctggaagaa 
      aaccaagcc ttacttgcc gaaaaccag gcccttaaa 
      gacaaagaa agcaaaggt tccgacctc cgaaaacag 
      aacgaagag ctcaaggag gaacttttc aaagccgaa 
      gtgatgcga gacgacttc aaagcgaaa atcaccgaa 
      caacaaaaa cagatcaaa gccaatcag gaaaaaatc 
      gccgaacta caactagcc gcaaacgac acttcccgc 
      ctgaaagac gaagtggac gctctgtcg gaatccgcc 
      gccaaggtc caagtcctt gaagcaaac ctcgcatcg 
      tacaagaaa aaactcgaa aagtacgcc gacgccaag 
      aaaacgctc cagaaactg gaagagaag aacatggaa 
      tacctgcag aaaatcctc caacacgag gaggaactg 
      gcgaaagtg accagctgg aaaagccag tgcgaaacc 
      tacaagaaa caactcatg gaaatggag cagaaactg 
      gacgaggag acgcaacgc gccgacaaa gcccagttc 
      aactgcgag aagcttgag aacaagttg gtcgctctc 
      cagggtgaa agagagcgg ataatccaa gaacgggac 
      gtcctgcgt gaggaaatg gacgagctg aagctgggc 
      cacccgaag aaggaggcg ggagcagcc atggcacag 
      gaactcacc ccaactgaa atgaaagaa cggctccgg 
      ttcttagaa aaagagaat aagacactc cggacgtct 
      ctgcaagag cacgaagcg aaccaagcc cttctcgag 
      agcgcccta gccagggtt gagaaactg caacaacag 
      aagcggtca gccaaccag accgttctg aagctggaa 
      gcgcagctc gaagagctg aagaataaa gtggcgagt 
      gatgagcca caacacgcc gatagttta gtgaaggag 
      tacaagcag aaaattgcg acgttacaa gaggctttg 
      acggtgaaa gaaagcgag cttcagacg gcgcaagcc 
      aagtacacc aggaatctg gagaaggcg agggaagtg 
      gcacaacaa ctggagccg aaaagtaac ggcggaatt 
      gattctttg aataacaca aggcatatg aaggagatg 
      gaagagcga ctgatggct gcggcgttt tataagctg 
      ggaatgacc tgccacagg gaggccatt gacgagagg 
      ttggccgtt ttgagcgcc ggccaagga cagtcgttt 
      ctggcgagg caaaggcaa ccaacgccc aggaaacaa 
      atccagagg tttaagtca aaataa
    mRNA sequence
    • >TC004151 RA
      gagttgtta attattgtc aaaatgtta agataatga 
      cagtgacat ttgacacga caatgtatt gataaatat 
      gtctcacaa ttatagggg tcaattttt acaaattct 
      tcccgtcta gtccattga taagcgaca tgtcacacg 
      gtacgataa aaatgaatc caagtagtg gaatgtgtg 
      aaagtttga taacttggt tccaaagta tagtgccaa 
      gtagagctc gaaatattg aagaaatat gtgatgggg 
      ttgcaatgt tagaagctt taatacaaa tagcaccgg 
      tccacttta gcaaattag aaccaaaaa taaagcgag 
      atgttagtt caagttgga ggctgcgtg tcagtaatt 
      taaagaaaa tttttgaag ctattagtg aatattatc 
      aggacgtcc tcaccctcc aactgcttg aagatgggc 
      gacctgatg tgactgaaa ttggggcca acaatgatc 
      ctgtgcaat tatccaagc ttttacggt taattttag 
      gatgtgcaa taaactgcg agagaaagc aagaataca 
      tcaccaaaa taatggaca tggaagaat cagtgcaac 
      aaaacataa aacaagcaa ttgaacagc tcgacttag 
      tgactgggg gccgttctg gcagtcttt taatcctcg 
      acagtgaca ccagagtgt cccagctag tcggcgaac 
      tcgaggttg caaacgaag ctaaagaga ccttgagtc 
      aacagtgcc aacaaatgg agacccagc tccacaacc 
      tcctggaag aaaaccaag ccttacttg ccgaaaacc 
      aggccctta aagacaaag aaagcaaag gttccgacc 
      tccgaaaac agaacgaag agctcaagg aggaacttt 
      tcaaagccg aagtgatgc gagacgact tcaaagcga 
      aaatcaccg aacaacaaa aacagatca aagccaatc 
      aggaaaaaa tcgccgaac tacaactag ccgcaaacg 
      acacttccc gcctgaaag acgaagtgg acgctctgt 
      cggaatccg ccgccaagg tccaagtcc ttgaagcaa 
      acctcgcat cgtacaaga aaaaactcg aaaagtacg 
      ccgacgcca agaaaacgc tccagaaac tggaagaga 
      agaacatgg aatacctgc agaaaatcc tccaacacg 
      aggaggaac tggcgaaag tgaccagct ggaaaagcc 
      agtgcgaaa cctacaaga aacaactca tggaaatgg 
      agcagaaac tggacgagg agacgcaac gcgccgaca 
      aagcccagt tcaactgcg agaagcttg agaacaagt 
      tggtcgctc tccagggtg aaagagagc ggataatcc 
      aagaacggg acgtcctgc gtgaggaaa tggacgagc 
      tgaagctgg gccacccga agaaggagg cgggagcag 
      ccatggcac aggaactca ccccaactg aaatgaaag 
      aacggctcc ggttcttag aaaaagaga ataagacac 
      tccggacgt ctctgcaag agcacgaag cgaaccaag 
      cccttctcg agagcgccc tagccaggg ttgagaaac 
      tgcaacaac agaagcggt cagccaacc agaccgttc 
      tgaagctgg aagcgcagc tcgaagagc tgaagaata 
      aagtggcga gtgatgagc cacaacacg ccgatagtt 
      tagtgaagg agtacaagc agaaaattg cgacgttac 
      aagaggctt tgacggtga aagaaagcg agcttcaga 
      cggcgcaag ccaagtaca ccaggaatc tggagaagg 
      cgagggaag tggcacaac aactggagc cgaaaagta 
      acggcggaa ttgattctt tgaataaca caaggcata 
      tgaaggaga tggaagagc gactgatgg ctgcggcgt 
      tttataagc tgggaatga cctgccaca gggaggcca 
      ttgacgaga ggttggccg ttttgagcg ccggccaag 
      gacagtcgt ttctggcga ggcaaaggc aaccaacgc 
      ccaggaaac aaatccaga ggtttaagt caaaataac 
      gtaatttac gttgtacta tttatcaat ctgctatat 
      tttaatcag tattttaat atattgttt atcgtattg 
      aaatacatt tgttgaaaa agt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY