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Gene information
General information
TC004146 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1666569..1673537
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004146
    iBeetle screen iB_00668

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004146 RA
      MAKVAYSPL TGFKHSAQN VSRIVLRSL PHNCKNYLC 
      NFNNLPIKG IDLNDILKE FTQKEVRHF FPFINKQIL 
      KLFRNNTDS RDGSFYYEM ITGFCTFED FQAEIWPYY 
      YPHLEEFQD GEFHQSLLK YWIPITVKH YKADFYDFI 
      AIKDADIYF KAHTLLEIK KRGFYKPQF NPDIMHFFN 
      WEKTSSVAL RMVCTFVSK KHHPEQSAV QMVQFYLNN 
      YSSFEFPAD VLKSLKIFI SQIFIVSHL TLAKSEQDL 
      SPQFLNFVY RFCLRRVSD PETCEVSAQ LLKVLWDIA 
      CGSSADLVT CYKIDLDCV YEVGGNFYF RQLQRSGFW 
      GDRFFNSEL TNKFAFLFG HKFYFCPKL VTLIKERYL 
      GVVECDFDL ATLLVQVNE LVCLQSSET IRIYTDLVV 
      AYRKLRKKP FSREIEKLC SIFNDTVEN FDFEKMFTS 
      VLILDMLIV TKSYDNIDL ERVIDRSLC CISKIINTK 
      LDTGLNAKE TKIFEAFIN NTVECLVTS ENDWPNFDK 
      ESIFTNLSK IITDSNLKR PVSLSVGAI QTLGSNRTL 
      KSLYHNKQS GKIRRNPET RLVIHALCL SDKNLTKPF 
      LVSSLNFLL EVLSNPLSP DLALSSSLH SLEVLVSDN 
      SIHQLTLPF AVPIIQNCI QLFNRTNWI VRNADLQLL 
      KSLIERFLG VSLDENIRP KTIEDLFIL FPTLVPHFY 
      AVLSQSELS DSAVITLLF FTESQIVNG SLIGQSLEG 
      DFDKFRHLF VNIMQKYSN NLGFLAIKA FTSLCALEN 
      IPAVISEIV SHIQRHFHG MRRNVLANL IKLLRELHQ 
      KYKQGCVQT DGIVIDEAI SGLIAYLKQ FDSFYFNLL 
      SLRLHSVDE IAVTLNREI NYENRIWLN NHVPHLLRN 
      VSPDRLAIF LDRVLQTPE FLQVRVLSI IVDRISEID 
      VAPIMQILV LKLGELPDC SKFLIMCYA KTILLCSEI 
      AELNCDLIA GIRQDFGTD SIYKMFIYV LVLSHCDNK 
      SDSDARFVT KCVGKYVDA IGDDEILGD LASTLTHLY 
      KCSSAGDRY NVMKMAFYL LLSKPTALE ICKFISLLT 
      KFHSSSVLF NFASLCDLA KLSTWVGDR TVALKLLGE 
      FYCYVNDLP EEEASDTYY LIENDLSLP KRYVKAMLV 
      SQVSEKLDL CVGLNRTSV RMFILQLLI YQLMALGTW 
      AELLSDVTI EKMFFQFQG AVNYFLGIA DVPNIGHDV 
      TFNLYTRKN PQVAQILPP SYPSLIPLS NFNPNLNTY 
      FVIHGYTDH KHREIIARL RLVLNNAED SNVIVVDWS 
      RLAAFIYFD AVNHTVPVG TYVGEFLSL LESNFIDLK 
      TVHLIGHSL GAHISGIAG AFVGGRVRR ITGLDPAGP 
      LFELIEERN ESLSLDKTD ALFVDVIHT DADEFGVTK 
      PIGDADFYP NEGTSPQPG CTNPLTVVS CSHIRSVEF 
      YTESVVNDY PFEARHCNV TRGDYTCLE EEPVHMGHY 
      LSLEARGVY FLETNAEPP FAKRYG
    CDS sequence
    • >TC004146 RA
      atggcaaaa gtggcgtat tctcctctg actggtttc 
      aaacacagc gctcaaaat gtcagtagg attgtatta 
      aggtcattg ccgcacaat tgtaagaat tatttgtgc 
      aattttaac aaccttcca attaaaggc atcgatttg 
      aacgatatt ttaaaagaa ttcacgcaa aaagaggtc 
      cgtcatttt ttccctttc attaacaag caaattttg 
      aaactcttt cgtaataac acggacagt cgtgacggc 
      tcgttttat tacgaaatg ataacaggc ttttgcaca 
      ttcgaggat ttccaagcg gaaatatgg ccctactat 
      tacccccat ttggaagaa ttccaagac ggggagttc 
      catcaaagt ttattaaaa tactggatt ccgataacc 
      gtgaagcat tacaaagcc gacttttac gattttatc 
      gcaatcaaa gacgctgat atttatttc aaagctcat 
      acgctgcta gaaatcaaa aaaaggggg ttttacaaa 
      ccgcagttc aaccccgac attatgcat ttttttaac 
      tgggaaaaa acaagctcg gtggcgctc cgcatggtt 
      tgcacgttc gtctcgaaa aaacatcac cccgaacaa 
      agtgcagta caaatggta cagttctac ctcaataat 
      tactcctcg tttgagttt cctgccgac gtcttaaaa 
      tcgctaaaa atatttatt tcgcaaata tttatagtc 
      tcccattta actcttgcc aaatctgag caagattta 
      agcccgcag ttcttgaat tttgtttac cgcttttgt 
      ttacgccgg gtttctgat ccagaaacg tgcgaggtt 
      tcggcccaa cttctgaaa gttctatgg gacattgcc 
      tgcggttcc tctgctgat ttagtaacg tgttataaa 
      attgatctg gactgcgtt tacgaagtc gggggcaat 
      ttctatttc cgacaatta caaagaagt ggcttttgg 
      ggcgatcgt ttttttaat tctgaactg accaacaag 
      tttgcgttc ctttttggc cacaagttt tatttttgc 
      cccaaacta gtcacactg attaaagaa cgttatttg 
      ggtgttgtg gagtgtgac tttgactta gcgacactt 
      ctggttcaa gtgaacgag ttagtgtgt ttgcaaagc 
      agtgaaacc attcgtatt tacaccgat ttggtggtt 
      gcttatcgc aaattaagg aaaaagccg ttttcacgt 
      gaaattgaa aaattatgt tcaattttc aacgacacg 
      gtggaaaat ttcgatttt gagaaaatg tttacttcg 
      gttctaatt ttggatatg cttattgta actaaaagt 
      tacgataat atcgacctg gagcgtgta attgacagg 
      agtttatgt tgcatttcg aaaattata aatacgaaa 
      cttgatacc ggcttgaac gctaaggaa acaaagatt 
      ttcgaagct ttcatcaac aacactgtc gaatgtttg 
      gtcacgtca gagaatgac tggccgaac tttgataag 
      gagagtatt ttcacaaat ctttcgaaa ataatcact 
      gatagtaac ttgaagcgg cccgtgtcg ttatcagtt 
      ggcgccatc caaactctg ggctcgaac cggacgcta 
      aagtcgctt tatcataat aaacaaagt ggaaaaatt 
      agacgaaat ccggaaacg agactcgtc attcacgct 
      ttgtgtctc tcggataag aatctaact aagccgttc 
      ctggtctca tctttaaat tttctgctt gaagtgctt 
      tctaaccca ttatcgccc gatttggcg ttatcctcg 
      tcgctgcac agcctcgaa gtgttagtt tccgacaat 
      tccatccac cagctcact cttcccttc gccgtgcca 
      ataatacaa aactgcatc caactgttc aatagaaca 
      aactggata gtgagaaac gccgacttg cagctgctg 
      aagtcgctg attgagcgc tttctcggg gtttccctt 
      gacgagaac atccggcca aagacaatc gaagacttg 
      tttatttta ttccccacc cttgtcccg cacttttac 
      gcggttttg tcccaaagc gagctcagt gatagcgct 
      gttatcaca ctcttgttt ttcacggaa tcgcaaatt 
      gtcaatggc tcgctcatt gggcagtcg ctcgagggc 
      gacttcgac aagtttaga cacttattc gtcaacatc 
      atgcagaag tattcgaac aatttgggt tttttggca 
      atcaaggct ttcacctcg ttgtgcgcc cttgaaaac 
      ataccggct gtcatctcg gagattgtc tcccacatc 
      cagagacac ttccacggg atgcgacga aacgtcctg 
      gcgaattta atcaaactc ctccgggag ttgcaccaa 
      aagtacaaa cagggctgt gtccagacg gacggaatt 
      gttattgac gaagccata agtggttta atcgcttat 
      ttaaaacaa tttgattcg ttttatttt aatttgttg 
      tccttgagg ctgcactcc gtcgatgaa atagcagtg 
      acactaaat cgggaaatt aattacgaa aaccgcatt 
      tggttaaat aaccacgtc ccgcacctc ttacgaaat 
      gtcagccca gacagatta gcaattttc ctggaccgt 
      gttttacaa acacctgag tttttacaa gtccgggtt 
      ttatcgatc atagttgat cgaattagc gagattgac 
      gttgcgcca ataatgcag attttggtt ctgaagttg 
      ggggaatta cccgattgt agcaaattc ctaattatg 
      tgctacgca aaaaccatt ttgttgtgt tccgagata 
      gcggagctg aattgcgac ctaatcgcc gggattaga 
      caggatttt gggaccgac tctatttac aaaatgttt 
      atttatgtt cttgtcttg tcacattgc gacaataag 
      tccgattcg gacgcgcgg tttgtcact aaatgtgtg 
      gggaaatac gttgatgca ataggggat gatgaaatt 
      ttgggcgac ttagcgtcc actcttaca cacttgtat 
      aaatgtagc tcggccggt gacaggtat aatgtgatg 
      aagatggcc ttctacttg ttgttaagc aaaccgacg 
      gctttggaa atttgtaaa tttatttcc ctattgacg 
      aaatttcac tcatcctct gtcctgttt aactttgcg 
      agcttgtgc gacttggcg aagttatca acgtgggta 
      ggcgataga acagtagct ttgaaacta ctgggggag 
      ttctattgc tacgttaac gatttgccc gaggaagag 
      gcttccgat acttactat ttgatcgaa aatgatttg 
      tcgctgcct aaacgatac gttaaggcg atgctagtt 
      agccaagtg agcgaaaaa ctcgatttg tgtgttggg 
      ttgaaccgg acaagtgtc agaatgttt attttacaa 
      ttactgata taccaatta atggcacta ggcacttgg 
      gccgaactg ctatcagac gtgacaatc gaaaaaatg 
      tttttccag tttcagggc gctgtcaat tacttttta 
      gggatcgct gatgttccc aacattggt cacgacgtt 
      acgttcaat ctctacacg agaaagaac ccgcaagtt 
      gcccagatc ttgcccccc agctacccc tctcttatc 
      cccttgtcc aacttcaac cccaacttg aacacctat 
      tttgtcatt catgggtac acagaccac aaacatcgg 
      gagattatt gctcgattg agacttgtg ttgaacaac 
      gccgaagac tcgaacgta attgtggtt gattggagc 
      cgtttggcg gcgtttatc tacttcgac gctgtgaac 
      cacaccgtt cctgtggga acatacgtt ggcgagttc 
      ctgtccctc ctggaatcg aattttatc gatctgaag 
      accgtgcat ttaatcggc cacagcctg ggggcgcac 
      atttcgggc atagctggc gcttttgtg ggaggccgc 
      gtgcggaga atcacggga ctggacccc gcaggcccc 
      ctgttcgag ctgattgag gagcgcaac gagtctctt 
      tcgcttgac aaaacggac gcgcttttc gtggacgtt 
      atccacacc gatgccgac gagttcggt gtaactaaa 
      ccgatcgga gacgctgat ttttaccca aatgagggg 
      acttcgccc cagccgggg tgcacgaac cccctgact 
      gttgtgtct tgtagccat ataagatcg gtggagttt 
      tacaccgag tcggttgtt aacgactat ccttttgag 
      gcaaggcac tgtaatgtt actcgaggc gactacacg 
      tgcttggag gaagaaccg gtccacatg ggtcattac 
      ctatcactt gaggcccga ggggtgtac ttcctggag 
      accaacgcc gagcccccc tttgctaaa cgttatggc 
      taa
    mRNA sequence
    • >TC004146 RA
      agttacctg ttgaaacac ttacagata tagaatcca 
      tttcaaagt tactgtttc atttagaag cgcaacttt 
      ttcaaataa attttgaca atgaatttg gtaacttgt 
      tacataacc tcaaaaatg acaactaca tcgtcttgt 
      caatagata aaaaaaaat cggttaaag tctttgtac 
      gtgagaagt ttatattca agatacttt tgtttgttt 
      cactgaagt acaggaagg ctttattca ctcagtcta 
      catcttcga taacgtttc ttcaattta ttttggaag 
      aaattctcg acactattg tcattgtca gaccccagt 
      ctttacatt tagttaaac tgctcgaga tggttttga 
      aaaaaaggg gcgatgtct tgactccta ccttatcag 
      tcttggttc taaaaacga tggcaaaag tggcgtatt 
      ctcctctga ctggtttca aacacagcg ctcaaaatg 
      tcagtagga ttgtattaa ggtcattgc cgcacaatt 
      gtaagaatt atttgtgca attttaaca accttccaa 
      ttaaaggca tcgatttga acgatattt taaaagaat 
      tcacgcaaa aagaggtcc gtcattttt tccctttca 
      ttaacaagc aaattttga aactctttc gtaataaca 
      cggacagtc gtgacggct cgttttatt acgaaatga 
      taacaggct tttgcacat tcgaggatt tccaagcgg 
      aaatatggc cctactatt acccccatt tggaagaat 
      tccaagacg gggagttcc atcaaagtt tattaaaat 
      actggattc cgataaccg tgaagcatt acaaagccg 
      acttttacg attttatcg caatcaaag acgctgata 
      tttatttca aagctcata cgctgctag aaatcaaaa 
      aaagggggt tttacaaac cgcagttca accccgaca 
      ttatgcatt tttttaact gggaaaaaa caagctcgg 
      tggcgctcc gcatggttt gcacgttcg tctcgaaaa 
      aacatcacc ccgaacaaa gtgcagtac aaatggtac 
      agttctacc tcaataatt actcctcgt ttgagtttc 
      ctgccgacg tcttaaaat cgctaaaaa tatttattt 
      cgcaaatat ttatagtct cccatttaa ctcttgcca 
      aatctgagc aagatttaa gcccgcagt tcttgaatt 
      ttgtttacc gcttttgtt tacgccggg tttctgatc 
      cagaaacgt gcgaggttt cggcccaac ttctgaaag 
      ttctatggg acattgcct gcggttcct ctgctgatt 
      tagtaacgt gttataaaa ttgatctgg actgcgttt 
      acgaagtcg ggggcaatt tctatttcc gacaattac 
      aaagaagtg gcttttggg gcgatcgtt tttttaatt 
      ctgaactga ccaacaagt ttgcgttcc tttttggcc 
      acaagtttt atttttgcc ccaaactag tcacactga 
      ttaaagaac gttatttgg gtgttgtgg agtgtgact 
      ttgacttag cgacacttc tggttcaag tgaacgagt 
      tagtgtgtt tgcaaagca gtgaaacca ttcgtattt 
      acaccgatt tggtggttg cttatcgca aattaagga 
      aaaagccgt tttcacgtg aaattgaaa aattatgtt 
      caattttca acgacacgg tggaaaatt tcgattttg 
      agaaaatgt ttacttcgg ttctaattt tggatatgc 
      ttattgtaa ctaaaagtt acgataata tcgacctgg 
      agcgtgtaa ttgacagga gtttatgtt gcatttcga 
      aaattataa atacgaaac ttgataccg gcttgaacg 
      ctaaggaaa caaagattt tcgaagctt tcatcaaca 
      acactgtcg aatgtttgg tcacgtcag agaatgact 
      ggccgaact ttgataagg agagtattt tcacaaatc 
      tttcgaaaa taatcactg atagtaact tgaagcggc 
      ccgtgtcgt tatcagttg gcgccatcc aaactctgg 
      gctcgaacc ggacgctaa agtcgcttt atcataata 
      aacaaagtg gaaaaatta gacgaaatc cggaaacga 
      gactcgtca ttcacgctt tgtgtctct cggataaga 
      atctaacta agccgttcc tggtctcat ctttaaatt 
      ttctgcttg aagtgcttt ctaacccat tatcgcccg 
      atttggcgt tatcctcgt cgctgcaca gcctcgaag 
      tgttagttt ccgacaatt ccatccacc agctcactc 
      ttcccttcg ccgtgccaa taatacaaa actgcatcc 
      aactgttca atagaacaa actggatag tgagaaacg 
      ccgacttgc agctgctga agtcgctga ttgagcgct 
      ttctcgggg tttcccttg acgagaaca tccggccaa 
      agacaatcg aagacttgt ttattttat tccccaccc 
      ttgtcccgc acttttacg cggttttgt cccaaagcg 
      agctcagtg atagcgctg ttatcacac tcttgtttt 
      tcacggaat cgcaaattg tcaatggct cgctcattg 
      ggcagtcgc tcgagggcg acttcgaca agtttagac 
      acttattcg tcaacatca tgcagaagt attcgaaca 
      atttgggtt ttttggcaa tcaaggctt tcacctcgt 
      tgtgcgccc ttgaaaaca taccggctg tcatctcgg 
      agattgtct cccacatcc agagacact tccacggga 
      tgcgacgaa acgtcctgg cgaatttaa tcaaactcc 
      tccgggagt tgcaccaaa agtacaaac agggctgtg 
      tccagacgg acggaattg ttattgacg aagccataa 
      gtggtttaa tcgcttatt taaaacaat ttgattcgt 
      tttatttta atttgttgt ccttgaggc tgcactccg 
      tcgatgaaa tagcagtga cactaaatc gggaaatta 
      attacgaaa accgcattt ggttaaata accacgtcc 
      cgcacctct tacgaaatg tcagcccag acagattag 
      caattttcc tggaccgtg ttttacaaa cacctgagt 
      ttttacaag tccgggttt tatcgatca tagttgatc 
      gaattagcg agattgacg ttgcgccaa taatgcaga 
      ttttggttc tgaagttgg gggaattac ccgattgta 
      gcaaattcc taattatgt gctacgcaa aaaccattt 
      tgttgtgtt ccgagatag cggagctga attgcgacc 
      taatcgccg ggattagac aggattttg ggaccgact 
      ctatttaca aaatgttta tttatgttc ttgtcttgt 
      cacattgcg acaataagt ccgattcgg acgcgcggt 
      ttgtcacta aatgtgtgg ggaaatacg ttgatgcaa 
      taggggatg atgaaattt tgggcgact tagcgtcca 
      ctcttacac acttgtata aatgtagct cggccggtg 
      acaggtata atgtgatga agatggcct tctacttgt 
      tgttaagca aaccgacgg ctttggaaa tttgtaaat 
      ttatttccc tattgacga aatttcact catcctctg 
      tcctgttta actttgcga gcttgtgcg acttggcga 
      agttatcaa cgtgggtag gcgatagaa cagtagctt 
      tgaaactac tgggggagt tctattgct acgttaacg 
      atttgcccg aggaagagg cttccgata cttactatt 
      tgatcgaaa atgatttgt cgctgccta aacgatacg 
      ttaaggcga tgctagtta gccaagtga gcgaaaaac 
      tcgatttgt gtgttgggt tgaaccgga caagtgtca 
      gaatgttta ttttacaat tactgatat accaattaa 
      tggcactag gcacttggg ccgaactgc tatcagacg 
      tgacaatcg aaaaaatgt ttttccagt ttcagggcg 
      ctgtcaatt actttttag ggatcgctg atgttccca 
      acattggtc acgacgtta cgttcaatc tctacacga 
      gaaagaacc cgcaagttg cccagatct tgcccccca 
      gctacccct ctcttatcc ccttgtcca acttcaacc 
      ccaacttga acacctatt ttgtcattc atgggtaca 
      cagaccaca aacatcggg agattattg ctcgattga 
      gacttgtgt tgaacaacg ccgaagact cgaacgtaa 
      ttgtggttg attggagcc gtttggcgg cgtttatct 
      acttcgacg ctgtgaacc acaccgttc ctgtgggaa 
      catacgttg gcgagttcc tgtccctcc tggaatcga 
      attttatcg atctgaaga ccgtgcatt taatcggcc 
      acagcctgg gggcgcaca tttcgggca tagctggcg 
      cttttgtgg gaggccgcg tgcggagaa tcacgggac 
      tggaccccg caggccccc tgttcgagc tgattgagg 
      agcgcaacg agtctcttt cgcttgaca aaacggacg 
      cgcttttcg tggacgtta tccacaccg atgccgacg 
      agttcggtg taactaaac cgatcggag acgctgatt 
      tttacccaa atgagggga cttcgcccc agccggggt 
      gcacgaacc ccctgactg ttgtgtctt gtagccata 
      taagatcgg tggagtttt acaccgagt cggttgtta 
      acgactatc cttttgagg caaggcact gtaatgtta 
      ctcgaggcg actacacgt gcttggagg aagaaccgg 
      tccacatgg gtcattacc tatcacttg aggcccgag 
      gggtgtact tcctggaga ccaacgccg agcccccct 
      ttgctaaac gttatggct aagattgta gagcttaaa 
      taaatgcgt gcgcgttga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY