General information | |||
TC004143 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 1621479..1627243 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC004143 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00667 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 4 (9 dpi)embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax fragmented - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC004143 RA
MLKFGSKHD SNIVYKCTV RLLEDTEIL ECEYKPHHK GKYLLEYVC QQLNLVEQD YLGLRYVDI QDQRHWLDL GKSICKQVK DLDPVLFSF RVKFYPPDP FRLKEEITR YQIFLQLKR DLLHGRLYC ATNEAAMLA ALILQGELG DYDPEIHVG NYMSELKIL LKQTETIEE KAMEIHQKQ LKGQSPSQV ENTFLKLAC QLDAYGVDP HPVKDHKGS QLYLGINYA GILTIQGSR KTHHFRWPD IQKINYEGK MFIIHLNYN DKKHTVGFK CPTGAACRH VWKCAVEQM LFFTLPASS DAPTVSGGG FFSWGTKFK YTGRTEKEI LEDSGSPTR EEPSIQRTS SLRRKASSV PATPSTPLQ PHLGYSSLP RSSHSDAGG SRMESSNST GLLSGLDGN PSQAYSDVA ALETVSEDQ EAMGASKLR TTVLKDDSM DRFSDYYFR DSFEHSSSE SQLIDSYRG YETTNPSHR SSRSQTPLS HSQVDGRIC LPNATHSVQ TNVKKTRKF NIVGAFIPT FIIVTIFVL IATVVMLET DCELFGSFR NIPEMVSLK YQFYEPIKQ YFRSKMSIF F
- >TC004143 RA
atgttaaag tttggctcc aaacatgac agtaatatt gtttacaaa tgcacggtc cgattattg gaagatacg gaaatccta gaatgtgaa tataagccc caccataag ggtaaatac ttgttggaa tatgtgtgt caacagttg aatttagta gaacaagac tacttaggg ctccgctat gttgatatt caagaccaa agacactgg ctggactta gggaaatca atctgtaaa caagtcaaa gatttggat ccggtcctg tttagtttt cgtgttaaa ttctaccct cctgatcca ttccgcctt aaggaagaa ataacacgt taccaaata tttttgcaa ttaaaacga gatctacta catgggcgc ttatattgc gccacgaac gaagccgca atgttggca gcacttatc ttacaaggt gaattgggc gattacgac cctgaaatt cacgttgga aattacatg agcgaatta aaaatacta cttaaacag actgaaacc atagaggag aaagcgatg gaaatccat cagaagcaa ctcaaaggc caaagcccc tcgcaagtg gaaaacacc ttcctcaaa ctggcctgc caactagac gcctacgga gttgacccg cacccagtg aaggaccat aaagggagt cagctctac ttgggcata aattatgcc ggaattttg acaattcaa ggtagtaga aaaacgcac cacttccgg tggcccgac atacagaaa atcaactac gagggaaaa atgttcata atccaccta aattacaat gataaaaag catacggtt ggttttaaa tgccccacg ggcgccgcc tgtcgccac gtttggaaa tgtgccgtt gaacaaatg ctatttttc actttgccg gcaagttcc gacgccccg actgtgagc ggcggtggg ttcttctca tggggtacg aagttcaaa tatacaggg cgtactgaa aaggaaatc ctggaagac agcggctcc ccaacacgg gaggagccc agcattcaa cgcacgtcc agtttgagg cgtaaagcg tccagtgta cctgcaact ccttcaacc ccccttcag ccccattta ggttatagt agtctgccg aggtcgagt cattcggac gccggaggc tcccgaatg gagtctagt aatagtaca gggttgctg tcagggtta gacgggaac ccgtcccaa gcttacagt gatgttgct gccttagaa actgtttcg gaagaccag gaggcgatg ggcgcctcc aaacttcga acaacagta ttgaaggat gattcgatg gatcgcttc agtgattat tatttccgg gactcgttc gaacattcg tcgtccgaa tcccagtta attgatagt taccgcggc tacgagact acgaatccc tctcatcgc tccagtcgt agccaaact ccgttatct cattctcag gttgatggt cgaatttgc ctgccaaat gctacacat agtgtacaa acaaatgtt aagaaaact agaaaattt aacatcgtg ggcgcgttc atacccacg ttcattata gtaacgata tttgtacta attgctacc gttgtaatg ttagaaact gattgtgaa ttatttggt agctttagg aacattccg gagatggtc tcgctaaaa taccaattc tatgaacca attaaacaa tattttcga agtaaaatg tcgatattt ttctga
- >TC004143 RA
tgtaaataa atgacgata cagacagat gtgctcgca gaaagagta tgtgaaggt attggcttt tttcaaggg gtgtacaat cggagtatg taatattga ttgcatttt tctggcgca gtacaaata aacgaatca ccctgtgta aaaaccagc ccttgtaac tttttacga aagttggcg aaattaatg gtggggcaa ttgctttta ttgggacat tttgtgatg aatgaaacg tagaaagtt ggcaatgaa gtgaactcg tgttgtgac tccgcccaa caggtgaac tatgcgcca aaatgggcg gggtttagc ggggaggag tattgcagt tgtgttgat tgttgtctt gttaaaaaa ataatttag gaattgttt tttcttggg atgatcgcc ggatttgtc ctttcaggc aaccgagtt gtctcgttg ttgtgggga gtttcgatg ttgttcatg cgcagctac gtcacgtag tattcaagc ttctgtcaa aaatatgtg tcaaatgct gtcttgtgt tatttacgt aaataattg tctgttgta aaataagat aataaattt tataaaaag ttgcgaatt ataaatgaa ctagacata tgtttaaag tggaaaatg ttaaagttt ggctccaaa catgacagt aatattgtt tacaaatgc acggtccga ttattggaa gatacggaa atcctagaa tgtgaatat aagccccac cataagggt aaatacttg ttggaatat gtgtgtcaa cagttgaat ttagtagaa caagactac ttagggctc cgctatgtt gatattcaa gaccaaaga cactggctg gacttaggg aaatcaatc tgtaaacaa gtcaaagat ttggatccg gtcctgttt agttttcgt gttaaattc taccctcct gatccattc cgccttaag gaagaaata acacgttac caaatattt ttgcaatta aaacgagat ctactacat gggcgctta tattgcgcc acgaacgaa gccgcaatg ttggcagca cttatctta caaggtgaa ttgggcgat tacgaccct gaaattcac gttggaaat tacatgagc gaattaaaa atactactt aaacagact gaaaccata gaggagaaa gcgatggaa atccatcag aagcaactc aaaggccaa agcccctcg caagtggaa aacaccttc ctcaaactg gcctgccaa ctagacgcc tacggagtt gacccgcac ccagtgaag gaccataaa gggagtcag ctctacttg ggcataaat tatgccgga attttgaca attcaaggt agtagaaaa acgcaccac ttccggtgg cccgacata cagaaaatc aactacgag ggaaaaatg ttcataatc cacctaaat tacaatgat aaaaagcat acggttggt tttaaatgc cccacgggc gccgcctgt cgccacgtt tggaaatgt gccgttgaa caaatgcta tttttcact ttgccggca agttccgac gccccgact gtgagcggc ggtgggttc ttctcatgg ggtacgaag ttcaaatat acagggcgt actgaaaag gaaatcctg gaagacagc ggctcccca acacgggag gagcccagc attcaacgc acgtccagt ttgaggcgt aaagcgtcc agtgtacct gcaactcct tcaaccccc cttcagccc catttaggt tatagtagt ctgccgagg tcgagtcat tcggacgcc ggaggctcc cgaatggag tctagtaat agtacaggg ttgctgtca gggttagac gggaacccg tcccaagct tacagtgat gttgctgcc ttagaaact gtttcggaa gaccaggag gcgatgggc gcctccaaa cttcgaaca acagtattg aaggatgat tcgatggat cgcttcagt gattattat ttccgggac tcgttcgaa cattcgtcg tccgaatcc cagttaatt gatagttac cgcggctac gagactacg aatccctct catcgctcc agtcgtagc caaactccg ttatctcat tctcaggtt gatggtcga atttgcctg ccaaatgct acacatagt gtacaaaca aatgttaag aaaactaga aaatttaac atcgtgggc gcgttcata cccacgttc attatagta acgatattt gtactaatt gctaccgtt gtaatgtta gaaactgat tgtgaatta tttggtagc tttaggaac attccggag atggtctcg ctaaaatac caattctat gaaccaatt aaacaatat tttcgaagt aaaatgtcg atatttttc tgattattt aagctctct catttgcat ttcgaattc agcttgaag aactctttg aagtagcaa cagattagt ttagttgca tagagtaag gaatccaat tattaaaat agttcctgc aatactaaa tcattagac taggttacg gtttcttta acgacaaaa tcagttttg tgttctaat ggctttaag caaaatgca ctgttttgt gatacattc gttgaacga actaaagtg taacattcc gttcagggc gcttcaacg ttattgtgg gcgttcctc attttttta atttattga acaaatgta caattttta tacaaaagt actcgggca gaaatattt tgttggcaa ttaaattta agaaatctg tgatctttt ctcaatctg ctgctatag taatagaaa taatcgtac aaagaagtg caatcaaaa tctattttc cgttgaatt tgtaactcg aatcataat tgctactta cgccaacaa tttgtgatt gtgttatac ttttgtgta cacagactt gcttagcga ggctatata attttgtat gtatgtatg atgatttaa ggggccaca gattcagaa gcacttagt tcatctatt tttatacgt tttattatt ttcaatttt atttttgtt atctttcta tgatgtaac tcatttcgc caaataaac ttaagtcaa ttaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
