Status
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Gene information
General information
TC004140 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1587008..1592203
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004140
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00666

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    vitellarium elongated - Number of animals showing the phenotype: 4
    egg chamber not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    remarks - cuticles: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 9

    wings partially blistered - Number of animals showing the phenotype: 8
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004140 RA
      MAGTDRVSL SDALSNVDV LDELPLPDE QPCIEAQPC 
SVVYQANFD TNFEDRNGF VTGIAKYIE EATVHASLN 
ELLEEGQEH AVMLYTWRC CSRAIPQPK SNEQPNRVE 
IYEKTVEVL APEVNKLLN FMYFQKKAI ERFFEEVKR 
LCHTEKRKD FVSEAYLLT LGKFINMFA VLDELKNMK 
SSVKNDYST YRRAAQFLK VMSDSQTLQ ESQNLSMFL 
ATQNKIRDA VKENLERIS GYEELLADV VNICVHMFE 
TKMYLTPSE KHMLVKVMG FGLFLMDSE LCNINKLDQ 
KKKLRLDRI DRIFKNLEV VPLFGDMQI APFNYIKRS 
KHFDPSKWP LSSSNTPSP QADLMVHLP QIRDDHVKY 
ISELARYSN EVTTTYKES GTDAENKET AELALRGLQ 
LLSEWTSVV TELYSWKLL HPTDHHQNK ECPQEAEEY 
ERATRYNYS DEEKFALIE VIAMIKGLQ VLMARMETV 
FTDAIRRNI YAELQDFVQ IILREPLRK AIKNKKDLI 
RSIIMSVRE TCADWQRGV EPTQDPALR GKKDDNGFN 
VKVPRRNVG PSSTQLYMV RTMLESLIA DKSGGKRTL 
RKDIDGQYL MQIDQFHKT SFYWNYLLS FSESLQQCC 
DLSQLWYRE FYLEMTMGR RINKCTVRH AHNEECNDL 
ITMEKRIQF PIDMSMPWI LTDHILKTK EPSMMEYVL 
YPLDLYNDS ALYALTIFR KQFLYDEVE AEVNLCFDQ 
FVYKLSEQI FAYYKQLAA SIFLDKRFR VECAALGAY 
LLPYPRANR YETLLKQRH VQLLGRSID LNKLITQRI 
NTDMQKSLD LAISKFEGG DITGVMELE GLLQVNRLC 
HKLLSKWLA LDDFDSMYR EANHNVLAP YGRITLHVF 
WELNYDFLP NYVYNAATN RFTKYRGQI AFAGAVHRD 
KPPQMSHHY MWGSKQLNL AYTTQYGQY SGFVGPQHF 
HTMCKLLGY QGIAVVMEE LLKIVKSLI QGNLLQFTK 
TLMEVMPKQ CKLPRYDYG SPGVLSYYH AQLNDIVQY 
PDARTELFH NFREFGNII LFCLLMEQA LSQEEVCDL 
LQAAPFQNI LPRPFCKEG EKPETKQKR LEAKYAALQ 
IVPNIEKLG TAKQSLISK EGDLLTRER LCCGLSIFE 
VVLNRLRTF LDDSVWVGP PPANGVMNV DECTEFHRL 
WSALQFVYC IPVSENEYT VEEMFGEGL HWAGCTMIV 
LLDQQRRFE ALDFCYHIL RVQRVDMKD EIVKGISLK 
RMVDRIRRF QVLNSQIFA ILNKFLKSS ENDELSVEH 
VRCFPPPVY PMNPQQHYH APEHLRQC
    CDS sequence
    • >TC004140 RA
      atggcagga actgatcga gtttcgctg agtgacgca 
ttatccaac gttgatgta ctagacgaa cttcctttg 
cctgatgaa caaccgtgc attgaggcc caaccttgc 
tcagttgtc tatcaggcg aattttgat acaaatttc 
gaagatcgg aacgggttt gtgacagga attgccaaa 
tacattgaa gaggcaaca gtacacgca agtctcaac 
gagttatta gaggaaggg caggagcat gctgtcatg 
ctctacacg tggaggtgc tgctctaga gccattcct 
cagcccaaa tctaacgaa caaccaaat cgggtcgaa 
atttacgag aaaactgtc gaagtgctc gcccccgaa 
gtgaacaaa ctcttgaac tttatgtat tttcaaaaa 
aaagcaatc gagcggttt ttcgaagaa gtaaaacga 
ttatgtcac accgaaaaa aggaaagac tttgtctca 
gaagcctat ctcctcact ttgggaaag ttcataaac 
atgtttgcc gtcttagac gagttaaaa aacatgaaa 
tcaagtgtc aaaaacgac tattccaca tacagaaga 
gcagcacaa tttttgaaa gtcatgtct gattcacaa 
acacttcaa gaatcacaa aatttatcg atgttctta 
gccacacaa aataaaatt agggatgct gttaaggag 
aatctggaa cgaatcagt ggctatgag gaattgcta 
gctgatgtt gtcaacatt tgcgtgcac atgtttgaa 
acgaaaatg tacctcaca cccagcgaa aaacacatg 
cttgttaaa gttatgggc ttcgggctt ttcctgatg 
gacagcgag ttgtgcaat attaataag ctagaccag 
aagaagaaa ttgaggctg gaccgcatt gaccggatt 
ttcaaaaac ttggaggtt gtccctttg ttcggggac 
atgcagatc gcccctttc aactacata aagcggagc 
aagcatttc gacccaagc aaatggcct ttatcaagt 
agcaatact ccctctccc caagccgat ttaatggta 
catttgccc caaattcgt gacgatcat gttaagtac 
ataagcgaa ttagcgcga tacagtaac gaagtcacg 
acaacatac aaagaaagc ggtactgac gctgaaaat 
aaagaaacg gccgaactt gcgctccgc ggattacaa 
ttactgtct gaatggaca agtgttgta acggaactg 
tattcatgg aagctttta cacccaact gaccaccat 
cagaataaa gagtgccca caagaggcc gaggaatac 
gaaagggcg acacggtat aattacagc gacgaggaa 
aaattcgcg ctaattgaa gtcattgcg atgattaag 
ggattacaa gtgctgatg gccagaatg gaaacggtg 
ttcactgac gcaattcgg cggaatatt tacgccgaa 
ttgcaagat ttcgtacag attattttg agggaaccg 
ctgaggaaa gcaattaaa aataaaaag gatcttatc 
agaagtatc attatgtcg gtgcgagaa acttgcgct 
gattggcag agaggtgtc gagccgact caagacccg 
gctttgagg ggcaaaaag gacgataac gggtttaat 
gttaaagtg ccaaggaga aacgtgggt ccttcctcc 
acccaactt tacatggtc aggacaatg ctggagtcg 
ctaattgcc gataaatca ggcggcaaa agaaccctg 
aggaaggac atagacggt caatattta atgcaaatt 
gaccagttc cacaaaacc tccttctac tggaattat 
cttctcagt ttcagtgaa tcgttgcag caatgttgc 
gacctgtcg cagctgtgg tacagagag ttctacctc 
gagatgacc atgggacgg agaataaac aaatgcacc 
gtgcgtcac gctcataac gaagaatgt aacgatttg 
atcacgatg gaaaaacgc atacaattc ccaatcgac 
atgtccatg ccttggatt ttgactgat catattttg 
aaaactaag gaaccctcg atgatggag tacgtcttg 
tacccgctt gatttatac aatgatagc gcgctttac 
gcactgaca atattccga aagcaattt ttatacgat 
gaggtcgag gctgaagtt aatttatgt tttgatcag 
tttgtttat aaactgagc gagcaaatt ttcgcgtac 
tataaacaa ctggcggca agtatcttt cttgataag 
cgcttcaga gtggagtgt gccgccttg ggggcttac 
ctgctcccc tacccgcga gcaaatcgt tacgaaacc 
ttgctaaaa cagcggcac gtccagtta ttagggcgg 
tcgatcgat ttgaataag ttgatcact cagaggatc 
aacaccgac atgcagaaa agtctcgat ttggcaata 
agtaagttc gaaggcggt gacataacc ggggttatg 
gaactggaa ggcctccta caagtgaat cggttgtgt 
cataaattg ctgagcaag tggctcgca cttgacgat 
ttcgattca atgtatcgt gaggctaat cacaacgtt 
ttggcgccc tatggccgc atcaccttg catgtgttt 
tgggagctg aactacgat ttcttgcct aattacgtc 
tacaatgcc gcgacgaac agatttact aagtatcgg 
gggcagatt gctttcgcg ggggctgtc cacagagat 
aagccgccg caaatgtcg catcattac atgtggggg 
agcaagcaa ctgaacctt gcctacacg actcagtat 
ggccaatat tccgggttc gtcgggcct cagcatttc 
cacacaatg tgcaaactc ttgggttat caaggcatt 
gcagttgtg atggaggag ctcctcaaa attgtcaaa 
agtctgatc caagggaac ctcctccaa ttcaccaaa 
actctaatg gaggtcatg ccaaagcag tgcaaattg 
ccacgttac gattacggc tcaccgggt gtgttaagt 
tactaccac gcccaattg aacgacatt gtccaatac 
cccgatgcc aggaccgag ttgttccac aatttccga 
gaatttggg aacattatc ctgttttgt ttactgatg 
gaacaggcc ttgtcacag gaggaagtc tgtgatttg 
ttgcaagcg gcaccgttt cagaatatc ctcccacgg 
ccgttttgc aaggaaggg gagaagccc gaaacgaag 
cagaaacgg cttgaagcc aaatatgcc gccttgcag 
attgtacca aatatagag aagttagga actgcaaag 
caatctttg atatcgaag gagggcgat ttattgacc 
cgtgagcgg ctgtgctgc ggtctctcg atttttgaa 
gttgttttg aaccgatta aggaccttc ctggacgat 
tcggtttgg gtcggaccg ccgcctgct aacggtgtc 
atgaacgtt gacgaatgc accgagttc catcgcttg 
tggtcggct ttacaattc gtctattgt attccagtt 
agcgagaac gaatatact gtcgaggaa atgtttggc 
gaaggtttg cattgggcg gggtgtaca atgatcgtg 
cttctggac cagcaaagg cggtttgag gccctcgat 
ttctgttac cacattctg agagtacaa agagttgac 
atgaaggat gaaattgtg aaaggcatt agcttgaag 
agaatggtg gacaggatc aggcgcttc caagtgctc 
aattcgcag atctttgcg attttgaac aagtttttg 
aagtcgagt gagaacgac gagttgtcg gttgaacat 
gtacgttgt ttcccgcca ccggtctac ccaatgaat 
ccgcaacag cactaccac gcacccgaa catctacgc 
cagtgttga
    mRNA sequence
    • >TC004140 RA
      tagttttat atttgtcat ttgtttact tgtctaatg 
ccgcaccta aaaatgtta aatatttct aaaatagca 
caaaattag ttaagtttg ttgttgata acaaatagc 
cacgttctg ggccatgtt tctaagaat tgattaaat 
tattttctc tctggaaaa acagcaagt ggagtgctt 
gaaatggca ggaactgat cgagtttcg ctgagtgac 
gcattatcc aacgttgat gtactagac gaacttcct 
ttgcctgat gaacaaccg tgcattgag gcccaacct 
tgctcagtt gtctatcag gcgaatttt gatacaaat 
ttcgaagat cggaacggg tttgtgaca ggaattgcc 
aaatacatt gaagaggca acagtacac gcaagtctc 
aacgagtta ttagaggaa gggcaggag catgctgtc 
atgctctac acgtggagg tgctgctct agagccatt 
cctcagccc aaatctaac gaacaacca aatcgggtc 
gaaatttac gagaaaact gtcgaagtg ctcgccccc 
gaagtgaac aaactcttg aactttatg tattttcaa 
aaaaaagca atcgagcgg tttttcgaa gaagtaaaa 
cgattatgt cacaccgaa aaaaggaaa gactttgtc 
tcagaagcc tatctcctc actttggga aagttcata 
aacatgttt gccgtctta gacgagtta aaaaacatg 
aaatcaagt gtcaaaaac gactattcc acatacaga 
agagcagca caatttttg aaagtcatg tctgattca 
caaacactt caagaatca caaaattta tcgatgttc 
ttagccaca caaaataaa attagggat gctgttaag 
gagaatctg gaacgaatc agtggctat gaggaattg 
ctagctgat gttgtcaac atttgcgtg cacatgttt 
gaaacgaaa atgtacctc acacccagc gaaaaacac 
atgcttgtt aaagttatg ggcttcggg cttttcctg 
atggacagc gagttgtgc aatattaat aagctagac 
cagaagaag aaattgagg ctggaccgc attgaccgg 
attttcaaa aacttggag gttgtccct ttgttcggg 
gacatgcag atcgcccct ttcaactac ataaagcgg 
agcaagcat ttcgaccca agcaaatgg cctttatca 
agtagcaat actccctct ccccaagcc gatttaatg 
gtacatttg ccccaaatt cgtgacgat catgttaag 
tacataagc gaattagcg cgatacagt aacgaagtc 
acgacaaca tacaaagaa agcggtact gacgctgaa 
aataaagaa acggccgaa cttgcgctc cgcggatta 
caattactg tctgaatgg acaagtgtt gtaacggaa 
ctgtattca tggaagctt ttacaccca actgaccac 
catcagaat aaagagtgc ccacaagag gccgaggaa 
tacgaaagg gcgacacgg tataattac agcgacgag 
gaaaaattc gcgctaatt gaagtcatt gcgatgatt 
aagggatta caagtgctg atggccaga atggaaacg 
gtgttcact gacgcaatt cggcggaat atttacgcc 
gaattgcaa gatttcgta cagattatt ttgagggaa 
ccgctgagg aaagcaatt aaaaataaa aaggatctt 
atcagaagt atcattatg tcggtgcga gaaacttgc 
gctgattgg cagagaggt gtcgagccg actcaagac 
ccggctttg aggggcaaa aaggacgat aacgggttt 
aatgttaaa gtgccaagg agaaacgtg ggtccttcc 
tccacccaa ctttacatg gtcaggaca atgctggag 
tcgctaatt gccgataaa tcaggcggc aaaagaacc 
ctgaggaag gacatagac ggtcaatat ttaatgcaa 
attgaccag ttccacaaa acctccttc tactggaat 
tatcttctc agtttcagt gaatcgttg cagcaatgt 
tgcgacctg tcgcagctg tggtacaga gagttctac 
ctcgagatg accatggga cggagaata aacaaatgc 
accgtgcgt cacgctcat aacgaagaa tgtaacgat 
ttgatcacg atggaaaaa cgcatacaa ttcccaatc 
gacatgtcc atgccttgg attttgact gatcatatt 
ttgaaaact aaggaaccc tcgatgatg gagtacgtc 
ttgtacccg cttgattta tacaatgat agcgcgctt 
tacgcactg acaatattc cgaaagcaa tttttatac 
gatgaggtc gaggctgaa gttaattta tgttttgat 
cagtttgtt tataaactg agcgagcaa attttcgcg 
tactataaa caactggcg gcaagtatc tttcttgat 
aagcgcttc agagtggag tgtgccgcc ttgggggct 
tacctgctc ccctacccg cgagcaaat cgttacgaa 
accttgcta aaacagcgg cacgtccag ttattaggg 
cggtcgatc gatttgaat aagttgatc actcagagg 
atcaacacc gacatgcag aaaagtctc gatttggca 
ataagtaag ttcgaaggc ggtgacata accggggtt 
atggaactg gaaggcctc ctacaagtg aatcggttg 
tgtcataaa ttgctgagc aagtggctc gcacttgac 
gatttcgat tcaatgtat cgtgaggct aatcacaac 
gttttggcg ccctatggc cgcatcacc ttgcatgtg 
ttttgggag ctgaactac gatttcttg cctaattac 
gtctacaat gccgcgacg aacagattt actaagtat 
cgggggcag attgctttc gcgggggct gtccacaga 
gataagccg ccgcaaatg tcgcatcat tacatgtgg 
gggagcaag caactgaac cttgcctac acgactcag 
tatggccaa tattccggg ttcgtcggg cctcagcat 
ttccacaca atgtgcaaa ctcttgggt tatcaaggc 
attgcagtt gtgatggag gagctcctc aaaattgtc 
aaaagtctg atccaaggg aacctcctc caattcacc 
aaaactcta atggaggtc atgccaaag cagtgcaaa 
ttgccacgt tacgattac ggctcaccg ggtgtgtta 
agttactac cacgcccaa ttgaacgac attgtccaa 
taccccgat gccaggacc gagttgttc cacaatttc 
cgagaattt gggaacatt atcctgttt tgtttactg 
atggaacag gccttgtca caggaggaa gtctgtgat 
ttgttgcaa gcggcaccg tttcagaat atcctccca 
cggccgttt tgcaaggaa ggggagaag cccgaaacg 
aagcagaaa cggcttgaa gccaaatat gccgccttg 
cagattgta ccaaatata gagaagtta ggaactgca 
aagcaatct ttgatatcg aaggagggc gatttattg 
acccgtgag cggctgtgc tgcggtctc tcgattttt 
gaagttgtt ttgaaccga ttaaggacc ttcctggac 
gattcggtt tgggtcgga ccgccgcct gctaacggt 
gtcatgaac gttgacgaa tgcaccgag ttccatcgc 
ttgtggtcg gctttacaa ttcgtctat tgtattcca 
gttagcgag aacgaatat actgtcgag gaaatgttt 
ggcgaaggt ttgcattgg gcggggtgt acaatgatc 
gtgcttctg gaccagcaa aggcggttt gaggccctc 
gatttctgt taccacatt ctgagagta caaagagtt 
gacatgaag gatgaaatt gtgaaaggc attagcttg 
aagagaatg gtggacagg atcaggcgc ttccaagtg 
ctcaattcg cagatcttt gcgattttg aacaagttt 
ttgaagtcg agtgagaac gacgagttg tcggttgaa 
catgtacgt tgtttcccg ccaccggtc tacccaatg 
aatccgcaa cagcactac cacgcaccc gaacatcta 
cgccagtgt tgaagcacg tttatttta tacatttgt 
tggaatcag ctttcaaca ttttataat cttttatat 
ttattatag tcctactac atgtgtaaa tctcattac 
aaatgtatc gcccaccct catttatta tcggcctta 
ttggtattg ctttaattt ttcataata aaattttcg 
atgcctg
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