Status
Please wait ...
Gene information
General information
TC004140 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1587008..1592203
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004140
    iBeetle screen iB_00666

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellarium elongated - Number of animals showing the phenotype: 4
    egg chamber not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    remarks - cuticles: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 9

    wings partially blistered - Number of animals showing the phenotype: 8
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004140 RA
      MAGTDRVSL SDALSNVDV LDELPLPDE QPCIEAQPC 
      SVVYQANFD TNFEDRNGF VTGIAKYIE EATVHASLN 
      ELLEEGQEH AVMLYTWRC CSRAIPQPK SNEQPNRVE 
      IYEKTVEVL APEVNKLLN FMYFQKKAI ERFFEEVKR 
      LCHTEKRKD FVSEAYLLT LGKFINMFA VLDELKNMK 
      SSVKNDYST YRRAAQFLK VMSDSQTLQ ESQNLSMFL 
      ATQNKIRDA VKENLERIS GYEELLADV VNICVHMFE 
      TKMYLTPSE KHMLVKVMG FGLFLMDSE LCNINKLDQ 
      KKKLRLDRI DRIFKNLEV VPLFGDMQI APFNYIKRS 
      KHFDPSKWP LSSSNTPSP QADLMVHLP QIRDDHVKY 
      ISELARYSN EVTTTYKES GTDAENKET AELALRGLQ 
      LLSEWTSVV TELYSWKLL HPTDHHQNK ECPQEAEEY 
      ERATRYNYS DEEKFALIE VIAMIKGLQ VLMARMETV 
      FTDAIRRNI YAELQDFVQ IILREPLRK AIKNKKDLI 
      RSIIMSVRE TCADWQRGV EPTQDPALR GKKDDNGFN 
      VKVPRRNVG PSSTQLYMV RTMLESLIA DKSGGKRTL 
      RKDIDGQYL MQIDQFHKT SFYWNYLLS FSESLQQCC 
      DLSQLWYRE FYLEMTMGR RINKCTVRH AHNEECNDL 
      ITMEKRIQF PIDMSMPWI LTDHILKTK EPSMMEYVL 
      YPLDLYNDS ALYALTIFR KQFLYDEVE AEVNLCFDQ 
      FVYKLSEQI FAYYKQLAA SIFLDKRFR VECAALGAY 
      LLPYPRANR YETLLKQRH VQLLGRSID LNKLITQRI 
      NTDMQKSLD LAISKFEGG DITGVMELE GLLQVNRLC 
      HKLLSKWLA LDDFDSMYR EANHNVLAP YGRITLHVF 
      WELNYDFLP NYVYNAATN RFTKYRGQI AFAGAVHRD 
      KPPQMSHHY MWGSKQLNL AYTTQYGQY SGFVGPQHF 
      HTMCKLLGY QGIAVVMEE LLKIVKSLI QGNLLQFTK 
      TLMEVMPKQ CKLPRYDYG SPGVLSYYH AQLNDIVQY 
      PDARTELFH NFREFGNII LFCLLMEQA LSQEEVCDL 
      LQAAPFQNI LPRPFCKEG EKPETKQKR LEAKYAALQ 
      IVPNIEKLG TAKQSLISK EGDLLTRER LCCGLSIFE 
      VVLNRLRTF LDDSVWVGP PPANGVMNV DECTEFHRL 
      WSALQFVYC IPVSENEYT VEEMFGEGL HWAGCTMIV 
      LLDQQRRFE ALDFCYHIL RVQRVDMKD EIVKGISLK 
      RMVDRIRRF QVLNSQIFA ILNKFLKSS ENDELSVEH 
      VRCFPPPVY PMNPQQHYH APEHLRQC
    CDS sequence
    • >TC004140 RA
      atggcagga actgatcga gtttcgctg agtgacgca 
      ttatccaac gttgatgta ctagacgaa cttcctttg 
      cctgatgaa caaccgtgc attgaggcc caaccttgc 
      tcagttgtc tatcaggcg aattttgat acaaatttc 
      gaagatcgg aacgggttt gtgacagga attgccaaa 
      tacattgaa gaggcaaca gtacacgca agtctcaac 
      gagttatta gaggaaggg caggagcat gctgtcatg 
      ctctacacg tggaggtgc tgctctaga gccattcct 
      cagcccaaa tctaacgaa caaccaaat cgggtcgaa 
      atttacgag aaaactgtc gaagtgctc gcccccgaa 
      gtgaacaaa ctcttgaac tttatgtat tttcaaaaa 
      aaagcaatc gagcggttt ttcgaagaa gtaaaacga 
      ttatgtcac accgaaaaa aggaaagac tttgtctca 
      gaagcctat ctcctcact ttgggaaag ttcataaac 
      atgtttgcc gtcttagac gagttaaaa aacatgaaa 
      tcaagtgtc aaaaacgac tattccaca tacagaaga 
      gcagcacaa tttttgaaa gtcatgtct gattcacaa 
      acacttcaa gaatcacaa aatttatcg atgttctta 
      gccacacaa aataaaatt agggatgct gttaaggag 
      aatctggaa cgaatcagt ggctatgag gaattgcta 
      gctgatgtt gtcaacatt tgcgtgcac atgtttgaa 
      acgaaaatg tacctcaca cccagcgaa aaacacatg 
      cttgttaaa gttatgggc ttcgggctt ttcctgatg 
      gacagcgag ttgtgcaat attaataag ctagaccag 
      aagaagaaa ttgaggctg gaccgcatt gaccggatt 
      ttcaaaaac ttggaggtt gtccctttg ttcggggac 
      atgcagatc gcccctttc aactacata aagcggagc 
      aagcatttc gacccaagc aaatggcct ttatcaagt 
      agcaatact ccctctccc caagccgat ttaatggta 
      catttgccc caaattcgt gacgatcat gttaagtac 
      ataagcgaa ttagcgcga tacagtaac gaagtcacg 
      acaacatac aaagaaagc ggtactgac gctgaaaat 
      aaagaaacg gccgaactt gcgctccgc ggattacaa 
      ttactgtct gaatggaca agtgttgta acggaactg 
      tattcatgg aagctttta cacccaact gaccaccat 
      cagaataaa gagtgccca caagaggcc gaggaatac 
      gaaagggcg acacggtat aattacagc gacgaggaa 
      aaattcgcg ctaattgaa gtcattgcg atgattaag 
      ggattacaa gtgctgatg gccagaatg gaaacggtg 
      ttcactgac gcaattcgg cggaatatt tacgccgaa 
      ttgcaagat ttcgtacag attattttg agggaaccg 
      ctgaggaaa gcaattaaa aataaaaag gatcttatc 
      agaagtatc attatgtcg gtgcgagaa acttgcgct 
      gattggcag agaggtgtc gagccgact caagacccg 
      gctttgagg ggcaaaaag gacgataac gggtttaat 
      gttaaagtg ccaaggaga aacgtgggt ccttcctcc 
      acccaactt tacatggtc aggacaatg ctggagtcg 
      ctaattgcc gataaatca ggcggcaaa agaaccctg 
      aggaaggac atagacggt caatattta atgcaaatt 
      gaccagttc cacaaaacc tccttctac tggaattat 
      cttctcagt ttcagtgaa tcgttgcag caatgttgc 
      gacctgtcg cagctgtgg tacagagag ttctacctc 
      gagatgacc atgggacgg agaataaac aaatgcacc 
      gtgcgtcac gctcataac gaagaatgt aacgatttg 
      atcacgatg gaaaaacgc atacaattc ccaatcgac 
      atgtccatg ccttggatt ttgactgat catattttg 
      aaaactaag gaaccctcg atgatggag tacgtcttg 
      tacccgctt gatttatac aatgatagc gcgctttac 
      gcactgaca atattccga aagcaattt ttatacgat 
      gaggtcgag gctgaagtt aatttatgt tttgatcag 
      tttgtttat aaactgagc gagcaaatt ttcgcgtac 
      tataaacaa ctggcggca agtatcttt cttgataag 
      cgcttcaga gtggagtgt gccgccttg ggggcttac 
      ctgctcccc tacccgcga gcaaatcgt tacgaaacc 
      ttgctaaaa cagcggcac gtccagtta ttagggcgg 
      tcgatcgat ttgaataag ttgatcact cagaggatc 
      aacaccgac atgcagaaa agtctcgat ttggcaata 
      agtaagttc gaaggcggt gacataacc ggggttatg 
      gaactggaa ggcctccta caagtgaat cggttgtgt 
      cataaattg ctgagcaag tggctcgca cttgacgat 
      ttcgattca atgtatcgt gaggctaat cacaacgtt 
      ttggcgccc tatggccgc atcaccttg catgtgttt 
      tgggagctg aactacgat ttcttgcct aattacgtc 
      tacaatgcc gcgacgaac agatttact aagtatcgg 
      gggcagatt gctttcgcg ggggctgtc cacagagat 
      aagccgccg caaatgtcg catcattac atgtggggg 
      agcaagcaa ctgaacctt gcctacacg actcagtat 
      ggccaatat tccgggttc gtcgggcct cagcatttc 
      cacacaatg tgcaaactc ttgggttat caaggcatt 
      gcagttgtg atggaggag ctcctcaaa attgtcaaa 
      agtctgatc caagggaac ctcctccaa ttcaccaaa 
      actctaatg gaggtcatg ccaaagcag tgcaaattg 
      ccacgttac gattacggc tcaccgggt gtgttaagt 
      tactaccac gcccaattg aacgacatt gtccaatac 
      cccgatgcc aggaccgag ttgttccac aatttccga 
      gaatttggg aacattatc ctgttttgt ttactgatg 
      gaacaggcc ttgtcacag gaggaagtc tgtgatttg 
      ttgcaagcg gcaccgttt cagaatatc ctcccacgg 
      ccgttttgc aaggaaggg gagaagccc gaaacgaag 
      cagaaacgg cttgaagcc aaatatgcc gccttgcag 
      attgtacca aatatagag aagttagga actgcaaag 
      caatctttg atatcgaag gagggcgat ttattgacc 
      cgtgagcgg ctgtgctgc ggtctctcg atttttgaa 
      gttgttttg aaccgatta aggaccttc ctggacgat 
      tcggtttgg gtcggaccg ccgcctgct aacggtgtc 
      atgaacgtt gacgaatgc accgagttc catcgcttg 
      tggtcggct ttacaattc gtctattgt attccagtt 
      agcgagaac gaatatact gtcgaggaa atgtttggc 
      gaaggtttg cattgggcg gggtgtaca atgatcgtg 
      cttctggac cagcaaagg cggtttgag gccctcgat 
      ttctgttac cacattctg agagtacaa agagttgac 
      atgaaggat gaaattgtg aaaggcatt agcttgaag 
      agaatggtg gacaggatc aggcgcttc caagtgctc 
      aattcgcag atctttgcg attttgaac aagtttttg 
      aagtcgagt gagaacgac gagttgtcg gttgaacat 
      gtacgttgt ttcccgcca ccggtctac ccaatgaat 
      ccgcaacag cactaccac gcacccgaa catctacgc 
      cagtgttga
    mRNA sequence
    • >TC004140 RA
      tagttttat atttgtcat ttgtttact tgtctaatg 
      ccgcaccta aaaatgtta aatatttct aaaatagca 
      caaaattag ttaagtttg ttgttgata acaaatagc 
      cacgttctg ggccatgtt tctaagaat tgattaaat 
      tattttctc tctggaaaa acagcaagt ggagtgctt 
      gaaatggca ggaactgat cgagtttcg ctgagtgac 
      gcattatcc aacgttgat gtactagac gaacttcct 
      ttgcctgat gaacaaccg tgcattgag gcccaacct 
      tgctcagtt gtctatcag gcgaatttt gatacaaat 
      ttcgaagat cggaacggg tttgtgaca ggaattgcc 
      aaatacatt gaagaggca acagtacac gcaagtctc 
      aacgagtta ttagaggaa gggcaggag catgctgtc 
      atgctctac acgtggagg tgctgctct agagccatt 
      cctcagccc aaatctaac gaacaacca aatcgggtc 
      gaaatttac gagaaaact gtcgaagtg ctcgccccc 
      gaagtgaac aaactcttg aactttatg tattttcaa 
      aaaaaagca atcgagcgg tttttcgaa gaagtaaaa 
      cgattatgt cacaccgaa aaaaggaaa gactttgtc 
      tcagaagcc tatctcctc actttggga aagttcata 
      aacatgttt gccgtctta gacgagtta aaaaacatg 
      aaatcaagt gtcaaaaac gactattcc acatacaga 
      agagcagca caatttttg aaagtcatg tctgattca 
      caaacactt caagaatca caaaattta tcgatgttc 
      ttagccaca caaaataaa attagggat gctgttaag 
      gagaatctg gaacgaatc agtggctat gaggaattg 
      ctagctgat gttgtcaac atttgcgtg cacatgttt 
      gaaacgaaa atgtacctc acacccagc gaaaaacac 
      atgcttgtt aaagttatg ggcttcggg cttttcctg 
      atggacagc gagttgtgc aatattaat aagctagac 
      cagaagaag aaattgagg ctggaccgc attgaccgg 
      attttcaaa aacttggag gttgtccct ttgttcggg 
      gacatgcag atcgcccct ttcaactac ataaagcgg 
      agcaagcat ttcgaccca agcaaatgg cctttatca 
      agtagcaat actccctct ccccaagcc gatttaatg 
      gtacatttg ccccaaatt cgtgacgat catgttaag 
      tacataagc gaattagcg cgatacagt aacgaagtc 
      acgacaaca tacaaagaa agcggtact gacgctgaa 
      aataaagaa acggccgaa cttgcgctc cgcggatta 
      caattactg tctgaatgg acaagtgtt gtaacggaa 
      ctgtattca tggaagctt ttacaccca actgaccac 
      catcagaat aaagagtgc ccacaagag gccgaggaa 
      tacgaaagg gcgacacgg tataattac agcgacgag 
      gaaaaattc gcgctaatt gaagtcatt gcgatgatt 
      aagggatta caagtgctg atggccaga atggaaacg 
      gtgttcact gacgcaatt cggcggaat atttacgcc 
      gaattgcaa gatttcgta cagattatt ttgagggaa 
      ccgctgagg aaagcaatt aaaaataaa aaggatctt 
      atcagaagt atcattatg tcggtgcga gaaacttgc 
      gctgattgg cagagaggt gtcgagccg actcaagac 
      ccggctttg aggggcaaa aaggacgat aacgggttt 
      aatgttaaa gtgccaagg agaaacgtg ggtccttcc 
      tccacccaa ctttacatg gtcaggaca atgctggag 
      tcgctaatt gccgataaa tcaggcggc aaaagaacc 
      ctgaggaag gacatagac ggtcaatat ttaatgcaa 
      attgaccag ttccacaaa acctccttc tactggaat 
      tatcttctc agtttcagt gaatcgttg cagcaatgt 
      tgcgacctg tcgcagctg tggtacaga gagttctac 
      ctcgagatg accatggga cggagaata aacaaatgc 
      accgtgcgt cacgctcat aacgaagaa tgtaacgat 
      ttgatcacg atggaaaaa cgcatacaa ttcccaatc 
      gacatgtcc atgccttgg attttgact gatcatatt 
      ttgaaaact aaggaaccc tcgatgatg gagtacgtc 
      ttgtacccg cttgattta tacaatgat agcgcgctt 
      tacgcactg acaatattc cgaaagcaa tttttatac 
      gatgaggtc gaggctgaa gttaattta tgttttgat 
      cagtttgtt tataaactg agcgagcaa attttcgcg 
      tactataaa caactggcg gcaagtatc tttcttgat 
      aagcgcttc agagtggag tgtgccgcc ttgggggct 
      tacctgctc ccctacccg cgagcaaat cgttacgaa 
      accttgcta aaacagcgg cacgtccag ttattaggg 
      cggtcgatc gatttgaat aagttgatc actcagagg 
      atcaacacc gacatgcag aaaagtctc gatttggca 
      ataagtaag ttcgaaggc ggtgacata accggggtt 
      atggaactg gaaggcctc ctacaagtg aatcggttg 
      tgtcataaa ttgctgagc aagtggctc gcacttgac 
      gatttcgat tcaatgtat cgtgaggct aatcacaac 
      gttttggcg ccctatggc cgcatcacc ttgcatgtg 
      ttttgggag ctgaactac gatttcttg cctaattac 
      gtctacaat gccgcgacg aacagattt actaagtat 
      cgggggcag attgctttc gcgggggct gtccacaga 
      gataagccg ccgcaaatg tcgcatcat tacatgtgg 
      gggagcaag caactgaac cttgcctac acgactcag 
      tatggccaa tattccggg ttcgtcggg cctcagcat 
      ttccacaca atgtgcaaa ctcttgggt tatcaaggc 
      attgcagtt gtgatggag gagctcctc aaaattgtc 
      aaaagtctg atccaaggg aacctcctc caattcacc 
      aaaactcta atggaggtc atgccaaag cagtgcaaa 
      ttgccacgt tacgattac ggctcaccg ggtgtgtta 
      agttactac cacgcccaa ttgaacgac attgtccaa 
      taccccgat gccaggacc gagttgttc cacaatttc 
      cgagaattt gggaacatt atcctgttt tgtttactg 
      atggaacag gccttgtca caggaggaa gtctgtgat 
      ttgttgcaa gcggcaccg tttcagaat atcctccca 
      cggccgttt tgcaaggaa ggggagaag cccgaaacg 
      aagcagaaa cggcttgaa gccaaatat gccgccttg 
      cagattgta ccaaatata gagaagtta ggaactgca 
      aagcaatct ttgatatcg aaggagggc gatttattg 
      acccgtgag cggctgtgc tgcggtctc tcgattttt 
      gaagttgtt ttgaaccga ttaaggacc ttcctggac 
      gattcggtt tgggtcgga ccgccgcct gctaacggt 
      gtcatgaac gttgacgaa tgcaccgag ttccatcgc 
      ttgtggtcg gctttacaa ttcgtctat tgtattcca 
      gttagcgag aacgaatat actgtcgag gaaatgttt 
      ggcgaaggt ttgcattgg gcggggtgt acaatgatc 
      gtgcttctg gaccagcaa aggcggttt gaggccctc 
      gatttctgt taccacatt ctgagagta caaagagtt 
      gacatgaag gatgaaatt gtgaaaggc attagcttg 
      aagagaatg gtggacagg atcaggcgc ttccaagtg 
      ctcaattcg cagatcttt gcgattttg aacaagttt 
      ttgaagtcg agtgagaac gacgagttg tcggttgaa 
      catgtacgt tgtttcccg ccaccggtc tacccaatg 
      aatccgcaa cagcactac cacgcaccc gaacatcta 
      cgccagtgt tgaagcacg tttatttta tacatttgt 
      tggaatcag ctttcaaca ttttataat cttttatat 
      ttattatag tcctactac atgtgtaaa tctcattac 
      aaatgtatc gcccaccct catttatta tcggcctta 
      ttggtattg ctttaattt ttcataata aaattttcg 
      atgcctg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY