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Gene information
General information
TC004121 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 1478194..1482593
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004121
    iBeetle screen iB_00664
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    Fertility
    egg production: number <50% of expected -
    vitellarium not present - Number of animals showing the phenotype: 3

    tropharium size decreased - Number of animals showing the phenotype: 3
    tropharium shape rounded - Number of animals showing the phenotype: 3
    egg chamber not present - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004121 RA
      MALVHRKFV MSAILSRFA RAYSTPVQE KDIRKSVFI 
      SQSKDIFTN LALEDWLYK NLDFTNHHV LMLWQNDPC 
      VVIGRHQNP WLEANVPAL GALTDQGVA LARRNSGGG 
      TVFHDQGNL NMTFFTSRN HYNRRYNLE VITRAIFRE 
      YGLKLEITP RDDLTLRNC KVSGTAAKL GRPSAYHHC 
      TLLVNTNKV HLSEALQKS DVGIETNAT KSTKSKILN 
      LCEENPKIK VPALYKVVG WEYLRTHAL SVKDGGMEL 
      ANQQKGFQM VNPTEKWFP GIEEIRDNL QGWQWRFGK 
      TPKFTISRS FTVPEHLIS QDVPDDLKV TMVVEGGKI 
      SDVNLYVPP GLVANGFSG NVNVITSLI GHKFSEEAL 
      DNLEWSLGA LGSDKDKFV TDCVKQVMQ SV
    CDS sequence
    • >TC004121 RA
      atggcacta gtgcaccgc aagttcgtc atgagtgcg 
      atcctctcg cgattcgcg cgcgcttac tccacccca 
      gtccaggaa aaagacatt cgaaaatca gtatttatc 
      tcgcagtcc aaagacatt ttcactaac ctagccctc 
      gaggactgg ctgtacaaa aacctcgac tttaccaac 
      caccatgtg ctaatgctg tggcagaac gacccctgc 
      gtggtcatc ggcaggcac cagaacccc tggctggag 
      gccaacgtg ccggccctc ggggccctc acggaccag 
      ggggtcgcc ctcgcccga cgcaacagc ggcgggggc 
      acggtcttc cacgaccaa ggcaacctc aatatgacc 
      ttcttcacc tccaggaat cattacaac aggaggtac 
      aacctcgaa gtcatcacc agggccatc ttcagggag 
      tacggcctc aaactcgag atcaccccc agagatgac 
      ctcacttta cgcaactgc aaagtgtcg ggcaccgcc 
      gcgaagctg gggcgcccc agtgcgtac caccactgc 
      accctgttg gtgaacacc aacaaggtg cacctgagc 
      gaggcgctg cagaagtcg gacgtggga atcgaaacc 
      aacgcgacc aagtccacc aaatcgaag atcctcaac 
      ttgtgtgag gagaacccg aagatcaaa gtgcccgct 
      ttgtacaaa gtggtgggt tgggagtac ttgcgcacc 
      cacgcgttg tcggtgaaa gacggcggc atggagctg 
      gccaaccag cagaagggc ttccagatg gtgaacccg 
      accgagaag tggttccca gggattgag gagattcgg 
      gacaatttg caaggctgg cagtggcgg ttcgggaaa 
      acccccaaa tttacgatc agccgctcg ttcaccgtc 
      cccgagcat ttgatcagc caggatgtg cctgatgat 
      ttgaaggtc accatggtg gtggaaggg gggaaaatc 
      agcgatgtg aacctgtat gttccaccc gggttggtg 
      gcaaacggt ttcagtggg aatgttaat gttataact 
      agtctcatt gggcacaaa ttttccgag gaagcctta 
      gataatctg gagtggtcg ctcggggcg cttggtagt 
      gataaggat aaattcgtt actgattgt gtaaagcaa 
      gtcatgcag tcggtatga
    mRNA sequence
    • >TC004121 RA
      ggggaacat aaacatcaa caggaaatt cgaaactaa 
      aaatatgaa acaaatggc aaatgaatg attttgtca 
      ttagcaacg attgcaaag gtcgtctgc agtgattat 
      aaatactgg aattgaatt gttctagat ttgattgtt 
      ttgtgttgg tgtcagggc cgtaattcc atacccgag 
      tggattcat ttatgttaa aatctgtct ggaattaat 
      ggtgagcga taactggta attccgtgt taatttaga 
      taataagtt tgttgacct ttgactgaa cctcgccag 
      gtttgggta atcactaat tacacctat caggttggg 
      aaaattcgg cctattttt aacgcgtgt cgagcgcgg 
      tggtaatca cgaattgga gagtgaacg ccgagaaaa 
      atcgggcga tagcggttt tgccgattt tacgtaaag 
      tgtcaatca catggtaac aaacgaaag gataggagt 
      taatctggg acttgtttt tctttgtct cccgataaa 
      gcatgaccc ctgtgaaga taaatgacc ccaagagcc 
      cggtttata tagaacgca aagggagga atgctgagt 
      actactaga gattgtctt ggtcttcga gtccgtctc 
      aacgccctg caccaaacc tggaagtgg ctgcaccag 
      accatcacc gtccacgag tacctcatc gccctgaca 
      gcgtcccat aaaaacgtt tttatgaaa tacggcccc 
      acctgttag agacattcg gcacaacac ctaaacctg 
      gcaaaatgg cactagtgc accgcaagt tcgtcatga 
      gtgcgatcc tctcgcgat tcgcgcgcg cttactcca 
      ccccagtcc aggaaaaag acattcgaa aatcagtat 
      ttatctcgc agtccaaag acattttca ctaacctag 
      ccctcgagg actggctgt acaaaaacc tcgacttta 
      ccaaccacc atgtgctaa tgctgtggc agaacgacc 
      cctgcgtgg tcatcggca ggcaccaga acccctggc 
      tggaggcca acgtgccgg ccctcgggg ccctcacgg 
      accaggggg tcgccctcg cccgacgca acagcggcg 
      ggggcacgg tcttccacg accaaggca acctcaata 
      tgaccttct tcacctcca ggaatcatt acaacagga 
      ggtacaacc tcgaagtca tcaccaggg ccatcttca 
      gggagtacg gcctcaaac tcgagatca cccccagag 
      atgacctca ctttacgca actgcaaag tgtcgggca 
      ccgccgcga agctggggc gccccagtg cgtaccacc 
      actgcaccc tgttggtga acaccaaca aggtgcacc 
      tgagcgagg cgctgcaga agtcggacg tgggaatcg 
      aaaccaacg cgaccaagt ccaccaaat cgaagatcc 
      tcaacttgt gtgaggaga acccgaaga tcaaagtgc 
      ccgctttgt acaaagtgg tgggttggg agtacttgc 
      gcacccacg cgttgtcgg tgaaagacg gcggcatgg 
      agctggcca accagcaga agggcttcc agatggtga 
      acccgaccg agaagtggt tcccaggga ttgaggaga 
      ttcgggaca atttgcaag gctggcagt ggcggttcg 
      ggaaaaccc ccaaattta cgatcagcc gctcgttca 
      ccgtccccg agcatttga tcagccagg atgtgcctg 
      atgatttga aggtcacca tggtggtgg aagggggga 
      aaatcagcg atgtgaacc tgtatgttc cacccgggt 
      tggtggcaa acggtttca gtgggaatg ttaatgtta 
      taactagtc tcattgggc acaaatttt ccgaggaag 
      ccttagata atctggagt ggtcgctcg gggcgcttg 
      gtagtgata aggataaat tcgttactg attgtgtaa 
      agcaagtca tgcagtcgg tatgacaga aagatgatt 
      aatctctcg gtccgaata tgttcctaa tattttcct 
      cagctagtg ccattttat tgtttttgt tttaattaa 
      attatcatc acattattt ttaactggt agataattg 
      tgttatttt ctgatattc gttatattt agattaagt 
      tcaaaaccg tttcgaacg gtcaacaag aattaatca 
      tatcattgt taacttaca ttattaata attgactaa 
      tttttttcg tttcttatt gtaatggag cggagacag 
      cctgaattt tgtaaataa acttagttt agtattttc 
      tttagttag ccagctttt tccttaatt tagtgcttg 
      tttaatttt ctgcatttt gcgttaaga tgttgtgcc 
      ttcaaatac atagtttta ataatccta gtgataagt 
      tgtgtaata gatgtttgg ataataaaa atatttttt 
      gaac