Status
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Gene information
General information
TC004103 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452207.1: 39910..43361
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004103
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00662

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    iBeetle screen iB_06671

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    mature egg not present - Number of animals showing the phenotype: 3
    previtellogenic egg chamber amount increased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 3

    follicular epithelium potentially anterior amount decreased - Number of animals showing the phenotype: 2
    previtellogenic egg chamber position irregular - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004103 RA
      MGKKRNEFS HITVEEKKV ITVAEIVQL LIKAQDEKR 
DVNLNALKA RISAKYGLG TSPRLVDII AAVPMNYRK 
TLVPKLLAK PIRTASGIA VVAVMCKPH RCPHINMTG 
NICVYCPGG PDSDFEYST QSYTGYEPT SMRAIRARY 
DPYLQTRHR VEQLKQLGH SVDKVEFIV MGGTFMSLP 
EDYRDYFIR NLHDALSGH KSSSVEEAV KYSERSNTK 
CIGITIETR PDYCLQKHL SDMLKYGCT RLEIGVQSV 
YEDVARDTN RGHTVKAVC ETFNLAKDA GYKVVSHMM 
PDLPNVDLE RDIEQFIEF FENPNFRAD GLKIYPTLV 
IRGTGLYEL WKTGRYKSY PPSILVDLI ANILALVPP 
WTRVYRVQR DIPMPLVSS GVEHGNLRE LALQRMKDL 
GLRCRDVRT REVGITEIH EKVRPNDVE LIRRDYYAN 
GGWETFLSY EDPEQDILI GLLRLRKCS KATTYRPEL 
LGECSIVRE LHVYGSVVP VSGRNIKKF QHQGYGTLL 
MQKAEDIAI KEHKSVKIA VISGVGTRN YYRKLGYEL 
EGPYMVKSL KPEFYKSVF SCVDGEPLH KTTEYYERK 
KWTLDDSEW LEQFDK
    CDS sequence
    • >TC004103 RA
      atggggaag aaacgaaac gaattttcc catattact 
gttgaagag aaaaaagta ataacagtg gctgaaatt 
gtgcaattg ctcataaaa gcccaagat gaaaaacgc 
gacgttaat cttaacgct ctcaaggcg cgaatttcg 
gcaaaatat gggcttggg acttcgccc cgtcttgtt 
gacattata gccgcagtt ccgatgaac tatcgcaag 
actttagta ccgaaacta ttagctaaa ccgatcagg 
actgccagt gggattgca gttgtggca gtgatgtgt 
aagccgcac agatgcccc catattaac atgactggc 
aatatttgt gtatattgt cccggtggt cccgattct 
gatttcgaa tattcgact cagagttac acaggctat 
gaaccaacg tcaatgagg gccataagg gccagatat 
gatccctat ttacaaact agacacaga gtcgagcaa 
ttgaaacaa ttaggacat tctgtagat aaagttgaa 
tttattgtg atggggggc acgtttatg agccttccg 
gaggactac agggattat ttcattaga aatttgcac 
gatgcttta tctggacat aaaagtagt tcagttgag 
gaagctgtc aagtattct gaaagatcc aacactaag 
tgtattgga attacgatt gagacaagg ccggattat 
tgcttgcaa aagcatttg tctgatatg ttgaaatat 
gggtgtaca aggcttgaa ataggtgtc cagtcggtt 
tatgaagac gtggccagg gataccaac cgtggtcac 
actgttaaa gcagtttgt gaaacattt aatttagct 
aaggatgca ggctataaa gtggtatca catatgatg 
cctgatttg ccaaatgtt gatctagag cgtgacatt 
gagcagttt attgagttt ttcgaaaat ccgaatttt 
agagctgac ggtttgaaa atttatcct actttggta 
attcgaggg actggtctc tacgaattg tggaaaacc 
ggacgttat aagagttat cctccttcg attcttgtt 
gatttgatt gcaaatatt ttggctttg gtgccgcct 
tggaccagg gtgtataga gtgcaacgc gatataccc 
atgcctctt gttagttca ggggtcgag catggaaac 
ctccgcgaa ttagccctc caaagaatg aaagatcta 
ggtctgaga tgtcgtgac gtgcgaact cgcgaagtc 
ggaataaca gaaatccat gaaaaagtg cgccccaac 
gatgttgaa ttaattaga agagattat tacgcaaat 
ggaggttgg gagacgttc ttaagttac gaagatccc 
gaacaagac attctaatc ggcctctta cgtttgcgt 
aaatgctcc aaagcaacg acttatcgt cccgaactt 
ttgggtgaa tgttcaata gttcgtgaa cttcacgtt 
tatggaagt gtggttcct gtgagtggt cgtaacatt 
aagaaattc caacatcaa ggttatggc actctccta 
atgcaaaaa gccgaagat attgcaata aaagagcac 
aaatctgtt aaaattgcg gttatttct ggagtcggg 
actaggaat tattacaga aaattgggg tacgagctt 
gaggggcct tacatggtc aagtcacta aagccagaa 
ttttacaaa agtgtcttc agttgtgtt gatggtgag 
ccattgcat aaaactaca gagtattat gaacgaaaa 
aagtggacg cttgatgat agtgaatgg ttggagcag 
tttgataaa taa
    mRNA sequence
    • >TC004103 RA
      acatttaat atttccaca taaccttac tttcacgtt 
tggcaaatg tgcttgtag aattaatta ttttttcat 
tacacgatg gggaagaaa cgaaacgaa ttttcccat 
attactgtt gaagagaaa aaagtaata acagtggct 
gaaattgtg caattgctc ataaaagcc caagatgaa 
aaacgcgac gttaatctt aacgctctc aaggcgcga 
atttcggca aaatatggg cttgggact tcgccccgt 
cttgttgac attatagcc gcagttccg atgaactat 
cgcaagact ttagtaccg aaactatta gctaaaccg 
atcaggact gccagtggg attgcagtt gtggcagtg 
atgtgtaag ccgcacaga tgcccccat attaacatg 
actggcaat atttgtgta tattgtccc ggtggtccc 
gattctgat ttcgaatat tcgactcag agttacaca 
ggctatgaa ccaacgtca atgagggcc ataagggcc 
agatatgat ccctattta caaactaga cacagagtc 
gagcaattg aaacaatta ggacattct gtagataaa 
gttgaattt attgtgatg gggggcacg tttatgagc 
cttccggag gactacagg gattatttc attagaaat 
ttgcacgat gctttatct ggacataaa agtagttca 
gttgaggaa gctgtcaag tattctgaa agatccaac 
actaagtgt attggaatt acgattgag acaaggccg 
gattattgc ttgcaaaag catttgtct gatatgttg 
aaatatggg tgtacaagg cttgaaata ggtgtccag 
tcggtttat gaagacgtg gccagggat accaaccgt 
ggtcacact gttaaagca gtttgtgaa acatttaat 
ttagctaag gatgcaggc tataaagtg gtatcacat 
atgatgcct gatttgcca aatgttgat ctagagcgt 
gacattgag cagtttatt gagtttttc gaaaatccg 
aattttaga gctgacggt ttgaaaatt tatcctact 
ttggtaatt cgagggact ggtctctac gaattgtgg 
aaaaccgga cgttataag agttatcct ccttcgatt 
cttgttgat ttgattgca aatattttg gctttggtg 
ccgccttgg accagggtg tatagagtg caacgcgat 
atacccatg cctcttgtt agttcaggg gtcgagcat 
ggaaacctc cgcgaatta gccctccaa agaatgaaa 
gatctaggt ctgagatgt cgtgacgtg cgaactcgc 
gaagtcgga ataacagaa atccatgaa aaagtgcgc 
cccaacgat gttgaatta attagaaga gattattac 
gcaaatgga ggttgggag acgttctta agttacgaa 
gatcccgaa caagacatt ctaatcggc ctcttacgt 
ttgcgtaaa tgctccaaa gcaacgact tatcgtccc 
gaacttttg ggtgaatgt tcaatagtt cgtgaactt 
cacgtttat ggaagtgtg gttcctgtg agtggtcgt 
aacattaag aaattccaa catcaaggt tatggcact 
ctcctaatg caaaaagcc gaagatatt gcaataaaa 
gagcacaaa tctgttaaa attgcggtt atttctgga 
gtcgggact aggaattat tacagaaaa ttggggtac 
gagcttgag gggccttac atggtcaag tcactaaag 
ccagaattt tacaaaagt gtcttcagt tgtgttgat 
ggtgagcca ttgcataaa actacagag tattatgaa 
cgaaaaaag tggacgctt gatgatagt gaatggttg 
gagcagttt gataaataa ataaaataa acgaagtgt 
tacaaaaga ttggggtgt ccttttatt tgtttccta 
atttttaat aaaactttc gtgtgccaa gtgtccaaa 
cggtggagc ttgtcatag tctgcgacc attttgtta 
aacgtttct tcgtccgcc tcttcaaca cattcaatg 
agttttttc aaaaactca aattgagcg gtgttttca 
aagtcgggg tatttctct aaatatgat tcaagcgca 
agttttgca ttaagtgag tccacacac aagtggcac 
agaccagct ttacacaaa ttgtctttg acattgtaa 
tgcaaaatt ggagttttc aaatcaaaa aacgccact 
tcttgaaaa atgtttacc gctttgtta taatcgtga 
aactctgaa gacgcgtat tctgcaact ttaaggagg 
cactttttg gccgacatg aaattgtcc tcaactttg 
taataatcg gctgcttgt tcgtaatgt tgaatcgcg 
ttttcgagt tccaaattt tgttcgtag atttctcca 
atgttgttg tgcagtttc gcggccgtg ataaagcgg 
cccaaatcg gtgtaaatc tgtattgct ttgagtagg 
tattttaca gcgccgttt gtgtcgtat ttatctaaa 
catttggct gcaaaaaca aaatcgttg gctgcttca 
gaccggttg ttttgctcc aagtttaaa tcgccagcc 
attcggaac gctgtgctt gcctgtagc cagtttttc 
gcaagtttg aataaactt cccgctttc tggtaacat 
tcgattgcg tcttcggtg cggtttcgg gaagagaaa 
aaaagtgat tggaaaaaa cctttgggg gacagtttt 
ttctccgct tcggatacc agctggcgg gcgcgttct 
tccgcgttg gtcatgttg attaacatt tgagcgaac 
tcatgacag ctgatttat tgacatttg tcaaaagag 
ctctaagtt tttttttaa tgaatcggg ggcagaaca 
ttaaaactc tgtcgaaaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY