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Gene information
General information
TC004103 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2079: 39911..43362
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004103
    iBeetle screen iB_00662
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    iBeetle screen iB_06671
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 3
    previtellogenic egg chamber amount increased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber not present - Number of animals showing the phenotype: 3

    follicular epithelium potentially anterior amount decreased - Number of animals showing the phenotype: 2
    previtellogenic egg chamber position irregular - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004103 RA
      MGKKRNEFS HITVEEKKV ITVAEIVQL LIKAQDEKR 
      DVNLNALKA RISAKYGLG TSPRLVDII AAVPMNYRK 
      TLVPKLLAK PIRTASGIA VVAVMCKPH RCPHINMTG 
      NICVYCPGG PDSDFEYST QSYTGYEPT SMRAIRARY 
      DPYLQTRHR VEQLKQLGH SVDKVEFIV MGGTFMSLP 
      EDYRDYFIR NLHDALSGH KSSSVEEAV KYSERSNTK 
      CIGITIETR PDYCLQKHL SDMLKYGCT RLEIGVQSV 
      YEDVARDTN RGHTVKAVC ETFNLAKDA GYKVVSHMM 
      PDLPNVDLE RDIEQFIEF FENPNFRAD GLKIYPTLV 
      IRGTGLYEL WKTGRYKSY PPSILVDLI ANILALVPP 
      WTRVYRVQR DIPMPLVSS GVEHGNLRE LALQRMKDL 
      GLRCRDVRT REVGITEIH EKVRPNDVE LIRRDYYAN 
      GGWETFLSY EDPEQDILI GLLRLRKCS KATTYRPEL 
      LGECSIVRE LHVYGSVVP VSGRNIKKF QHQGYGTLL 
      MQKAEDIAI KEHKSVKIA VISGVGTRN YYRKLGYEL 
      EGPYMVKSL KPEFYKSVF SCVDGEPLH KTTEYYERK 
      KWTLDDSEW LEQFDK
    CDS sequence
    • >TC004103 RA
      atggggaag aaacgaaac gaattttcc catattact 
      gttgaagag aaaaaagta ataacagtg gctgaaatt 
      gtgcaattg ctcataaaa gcccaagat gaaaaacgc 
      gacgttaat cttaacgct ctcaaggcg cgaatttcg 
      gcaaaatat gggcttggg acttcgccc cgtcttgtt 
      gacattata gccgcagtt ccgatgaac tatcgcaag 
      actttagta ccgaaacta ttagctaaa ccgatcagg 
      actgccagt gggattgca gttgtggca gtgatgtgt 
      aagccgcac agatgcccc catattaac atgactggc 
      aatatttgt gtatattgt cccggtggt cccgattct 
      gatttcgaa tattcgact cagagttac acaggctat 
      gaaccaacg tcaatgagg gccataagg gccagatat 
      gatccctat ttacaaact agacacaga gtcgagcaa 
      ttgaaacaa ttaggacat tctgtagat aaagttgaa 
      tttattgtg atggggggc acgtttatg agccttccg 
      gaggactac agggattat ttcattaga aatttgcac 
      gatgcttta tctggacat aaaagtagt tcagttgag 
      gaagctgtc aagtattct gaaagatcc aacactaag 
      tgtattgga attacgatt gagacaagg ccggattat 
      tgcttgcaa aagcatttg tctgatatg ttgaaatat 
      gggtgtaca aggcttgaa ataggtgtc cagtcggtt 
      tatgaagac gtggccagg gataccaac cgtggtcac 
      actgttaaa gcagtttgt gaaacattt aatttagct 
      aaggatgca ggctataaa gtggtatca catatgatg 
      cctgatttg ccaaatgtt gatctagag cgtgacatt 
      gagcagttt attgagttt ttcgaaaat ccgaatttt 
      agagctgac ggtttgaaa atttatcct actttggta 
      attcgaggg actggtctc tacgaattg tggaaaacc 
      ggacgttat aagagttat cctccttcg attcttgtt 
      gatttgatt gcaaatatt ttggctttg gtgccgcct 
      tggaccagg gtgtataga gtgcaacgc gatataccc 
      atgcctctt gttagttca ggggtcgag catggaaac 
      ctccgcgaa ttagccctc caaagaatg aaagatcta 
      ggtctgaga tgtcgtgac gtgcgaact cgcgaagtc 
      ggaataaca gaaatccat gaaaaagtg cgccccaac 
      gatgttgaa ttaattaga agagattat tacgcaaat 
      ggaggttgg gagacgttc ttaagttac gaagatccc 
      gaacaagac attctaatc ggcctctta cgtttgcgt 
      aaatgctcc aaagcaacg acttatcgt cccgaactt 
      ttgggtgaa tgttcaata gttcgtgaa cttcacgtt 
      tatggaagt gtggttcct gtgagtggt cgtaacatt 
      aagaaattc caacatcaa ggttatggc actctccta 
      atgcaaaaa gccgaagat attgcaata aaagagcac 
      aaatctgtt aaaattgcg gttatttct ggagtcggg 
      actaggaat tattacaga aaattgggg tacgagctt 
      gaggggcct tacatggtc aagtcacta aagccagaa 
      ttttacaaa agtgtcttc agttgtgtt gatggtgag 
      ccattgcat aaaactaca gagtattat gaacgaaaa 
      aagtggacg cttgatgat agtgaatgg ttggagcag 
      tttgataaa taa
    mRNA sequence
    • >TC004103 RA
      acatttaat atttccaca taaccttac tttcacgtt 
      tggcaaatg tgcttgtag aattaatta ttttttcat 
      tacacgatg gggaagaaa cgaaacgaa ttttcccat 
      attactgtt gaagagaaa aaagtaata acagtggct 
      gaaattgtg caattgctc ataaaagcc caagatgaa 
      aaacgcgac gttaatctt aacgctctc aaggcgcga 
      atttcggca aaatatggg cttgggact tcgccccgt 
      cttgttgac attatagcc gcagttccg atgaactat 
      cgcaagact ttagtaccg aaactatta gctaaaccg 
      atcaggact gccagtggg attgcagtt gtggcagtg 
      atgtgtaag ccgcacaga tgcccccat attaacatg 
      actggcaat atttgtgta tattgtccc ggtggtccc 
      gattctgat ttcgaatat tcgactcag agttacaca 
      ggctatgaa ccaacgtca atgagggcc ataagggcc 
      agatatgat ccctattta caaactaga cacagagtc 
      gagcaattg aaacaatta ggacattct gtagataaa 
      gttgaattt attgtgatg gggggcacg tttatgagc 
      cttccggag gactacagg gattatttc attagaaat 
      ttgcacgat gctttatct ggacataaa agtagttca 
      gttgaggaa gctgtcaag tattctgaa agatccaac 
      actaagtgt attggaatt acgattgag acaaggccg 
      gattattgc ttgcaaaag catttgtct gatatgttg 
      aaatatggg tgtacaagg cttgaaata ggtgtccag 
      tcggtttat gaagacgtg gccagggat accaaccgt 
      ggtcacact gttaaagca gtttgtgaa acatttaat 
      ttagctaag gatgcaggc tataaagtg gtatcacat 
      atgatgcct gatttgcca aatgttgat ctagagcgt 
      gacattgag cagtttatt gagtttttc gaaaatccg 
      aattttaga gctgacggt ttgaaaatt tatcctact 
      ttggtaatt cgagggact ggtctctac gaattgtgg 
      aaaaccgga cgttataag agttatcct ccttcgatt 
      cttgttgat ttgattgca aatattttg gctttggtg 
      ccgccttgg accagggtg tatagagtg caacgcgat 
      atacccatg cctcttgtt agttcaggg gtcgagcat 
      ggaaacctc cgcgaatta gccctccaa agaatgaaa 
      gatctaggt ctgagatgt cgtgacgtg cgaactcgc 
      gaagtcgga ataacagaa atccatgaa aaagtgcgc 
      cccaacgat gttgaatta attagaaga gattattac 
      gcaaatgga ggttgggag acgttctta agttacgaa 
      gatcccgaa caagacatt ctaatcggc ctcttacgt 
      ttgcgtaaa tgctccaaa gcaacgact tatcgtccc 
      gaacttttg ggtgaatgt tcaatagtt cgtgaactt 
      cacgtttat ggaagtgtg gttcctgtg agtggtcgt 
      aacattaag aaattccaa catcaaggt tatggcact 
      ctcctaatg caaaaagcc gaagatatt gcaataaaa 
      gagcacaaa tctgttaaa attgcggtt atttctgga 
      gtcgggact aggaattat tacagaaaa ttggggtac 
      gagcttgag gggccttac atggtcaag tcactaaag 
      ccagaattt tacaaaagt gtcttcagt tgtgttgat 
      ggtgagcca ttgcataaa actacagag tattatgaa 
      cgaaaaaag tggacgctt gatgatagt gaatggttg 
      gagcagttt gataaataa ataaaataa acgaagtgt 
      tacaaaaga ttggggtgt ccttttatt tgtttccta 
      atttttaat aaaactttc gtgtgccaa gtgtccaaa 
      cggtggagc ttgtcatag tctgcgacc attttgtta 
      aacgtttct tcgtccgcc tcttcaaca cattcaatg 
      agttttttc aaaaactca aattgagcg gtgttttca 
      aagtcgggg tatttctct aaatatgat tcaagcgca 
      agttttgca ttaagtgag tccacacac aagtggcac 
      agaccagct ttacacaaa ttgtctttg acattgtaa 
      tgcaaaatt ggagttttc aaatcaaaa aacgccact 
      tcttgaaaa atgtttacc gctttgtta taatcgtga 
      aactctgaa gacgcgtat tctgcaact ttaaggagg 
      cactttttg gccgacatg aaattgtcc tcaactttg 
      taataatcg gctgcttgt tcgtaatgt tgaatcgcg 
      ttttcgagt tccaaattt tgttcgtag atttctcca 
      atgttgttg tgcagtttc gcggccgtg ataaagcgg 
      cccaaatcg gtgtaaatc tgtattgct ttgagtagg 
      tattttaca gcgccgttt gtgtcgtat ttatctaaa 
      catttggct gcaaaaaca aaatcgttg gctgcttca 
      gaccggttg ttttgctcc aagtttaaa tcgccagcc 
      attcggaac gctgtgctt gcctgtagc cagtttttc 
      gcaagtttg aataaactt cccgctttc tggtaacat 
      tcgattgcg tcttcggtg cggtttcgg gaagagaaa 
      aaaagtgat tggaaaaaa cctttgggg gacagtttt 
      ttctccgct tcggatacc agctggcgg gcgcgttct 
      tccgcgttg gtcatgttg attaacatt tgagcgaac 
      tcatgacag ctgatttat tgacatttg tcaaaagag 
      ctctaagtt tttttttaa tgaatcggg ggcagaaca 
      ttaaaactc tgtcgaaaa c