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Gene information
General information
TC004088 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450468.1: 17..6259
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004088
    iBeetle screen iB_00661

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004088 RA
      MKDEEDRAV ENWKGEVKM STSTEIEKQ IFTAIESGD 
      AVLLRTALA SKPDVNILD ENAMTPLQH AAYKGNKDM 
      VQMLLDYGA DPNLCKHQH NYTALHFAG LSGNSDVCL 
      ALLVAGAFP EVTNAVNRT ASQMAAFVG HHKCVAIIN 
      NYIPKSEVD YYSRLQGAQ TEPYLPPFL SESFHKLII 
      QVNVHPVKI ALNINSFIG LSQHLAKIK KVLELMYDK 
      EMQRGAGNN EVMAFKLYY LAFIVGEVM TIQNKHQSP 
      DATLQTFCK KVLKPATDG ALEILDGLL RECIKHFPF 
      RECTLFRQM VTTLTGKDT PTALSVINS AINGQRGFV 
      DNVPICYTC GEEKPSKKC SQCKVAQYC DKTCQKLHW 
      CWHKKACPQ LAQEPLKCN NDTQIDPQE LSSEIQNLL 
      VTN
    • >TC004088 RB
      MSTSTEIEK QIFTAIESG DAVLLRTAL ASKPDVNIL 
      DENAMTPLQ HAAYKGNKD MVQMLLDYG ADPNLCKHQ 
      HNYTALHFA GLSGNSDVC LALLVAGAF PEVTNAVNR 
      TASQMAAFV GHHKCVAII NNYIPKSEV DYYSRLQGA 
      QTEPYLPPF LSESFHKLI IQVNVHPVK IALNINSFI 
      GLSQHLAKI KKVLELMYD KEMQRGAGN NEVMAFKLY 
      YLAFIVGEV MTIQNKHQS PDATLQTFC KKVLKPATD 
      GALEILDGL LRECIKHFP FRECTLFRQ MVTTLTGKD 
      TPTALSVIN SAINGQRGF VDNVPICYT CGEEKPSKK 
      CSQCKVAQY CDKTCQKLH WCWHKKACP QLAQEPLKC 
      NNDTQIDPQ ELSSEIQNL LVTN
    CDS sequence
    • >TC004088 RA
      atgaaagat gaagaagat agagcagtt gaaaactgg 
      aaaggggaa gtaaaaatg tccacaagc accgaaatt 
      gagaagcaa atcttcact gcgatcgaa tccggagac 
      gctgtttta ctccgaacc gcattagca tccaaacct 
      gacgtaaac atcctagat gaaaatgca atgacacca 
      ctgcagcat gctgcttat aagggcaac aaggacatg 
      gtccaaatg ctcctcgac tatggggca gacccaaac 
      ctttgcaaa caccaacac aattacaca gcgctacat 
      ttcgcgggc ctttcgggc aattccgac gtgtgtctg 
      gcactgctc gtggctggg gccttcccc gaagtcacg 
      aacgcagtg aatcggacg gcgtcacaa atggcggcc 
      tttgtcggc catcataag tgcgtcgcc attatcaat 
      aattacatc ccaaagtca gaagtagat tattatagt 
      aggctacaa ggcgctcaa acggagcca tatttgccg 
      ccatttttg tccgaaagc ttccacaaa ttaataata 
      caagtaaac gtacacccg gtcaaaatc gcgctaaac 
      atcaacagt tttatcggg ttatcgcaa cacttggcg 
      aaaatcaaa aaagtgcta gaattgatg tacgacaaa 
      gaaatgcaa agaggggcc ggtaataac gaagtgatg 
      gcgttcaaa ctatactac ctggcgttt atagtaggc 
      gaggttatg acgattcaa aacaaacac caatcgcct 
      gacgccacg ttgcaaacg ttttgcaaa aaagtgcta 
      aaaccggct acagatggc gctttagaa atactcgat 
      gggttgttg cgggagtgc attaaacat ttcccattt 
      cgggagtgt acacttttt cgccaaatg gttacaaca 
      ctaacgggg aaagacacc ccaacagct ctaagtgtt 
      atcaattcg gccataaat ggtcagaga gggttcgtt 
      gataacgtc ccgatttgt tacacttgt ggggaagag 
      aaacctagt aagaagtgc tcgcaatgt aaagtggcg 
      cagtattgt gataagacg tgccaaaag ttgcactgg 
      tgttggcac aaaaaggcg tgcccacag ttggctcaa 
      gagcccctc aaatgtaat aatgacaca caaattgat 
      cctcaggaa ctgtcttca gaaatacag aatctttta 
      gttactaat taa
    • >TC004088 RB
      atgtccaca agcaccgaa attgagaag caaatcttc 
      actgcgatc gaatccgga gacgctgtt ttactccga 
      accgcatta gcatccaaa cctgacgta aacatccta 
      gatgaaaat gcaatgaca ccactgcag catgctgct 
      tataagggc aacaaggac atggtccaa atgctcctc 
      gactatggg gcagaccca aacctttgc aaacaccaa 
      cacaattac acagcgcta catttcgcg ggcctttcg 
      ggcaattcc gacgtgtgt ctggcactg ctcgtggct 
      ggggccttc cccgaagtc acgaacgca gtgaatcgg 
      acggcgtca caaatggcg gcctttgtc ggccatcat 
      aagtgcgtc gccattatc aataattac atcccaaag 
      tcagaagta gattattat agtaggcta caaggcgct 
      caaacggag ccatatttg ccgccattt ttgtccgaa 
      agcttccac aaattaata atacaagta aacgtacac 
      ccggtcaaa atcgcgcta aacatcaac agttttatc 
      gggttatcg caacacttg gcgaaaatc aaaaaagtg 
      ctagaattg atgtacgac aaagaaatg caaagaggg 
      gccggtaat aacgaagtg atggcgttc aaactatac 
      tacctggcg tttatagta ggcgaggtt atgacgatt 
      caaaacaaa caccaatcg cctgacgcc acgttgcaa 
      acgttttgc aaaaaagtg ctaaaaccg gctacagat 
      ggcgcttta gaaatactc gatgggttg ttgcgggag 
      tgcattaaa catttccca tttcgggag tgtacactt 
      tttcgccaa atggttaca acactaacg gggaaagac 
      accccaaca gctctaagt gttatcaat tcggccata 
      aatggtcag agagggttc gttgataac gtcccgatt 
      tgttacact tgtggggaa gagaaacct agtaagaag 
      tgctcgcaa tgtaaagtg gcgcagtat tgtgataag 
      acgtgccaa aagttgcac tggtgttgg cacaaaaag 
      gcgtgccca cagttggct caagagccc ctcaaatgt 
      aataatgac acacaaatt gatcctcag gaactgtct 
      tcagaaata cagaatctt ttagttact aattaa
    mRNA sequence
    • >TC004088 RA
      gaccttctt tctttgtca gagaaaaca catccaaat 
      tccaaagaa gtggaaaca acaaacgat tacaacgac 
      gagacaatg aaagatgaa gaagataga gcagttgaa 
      aactggaaa ggggaagta aaaatgtcc acaagcacc 
      gaaattgag aagcaaatc ttcactgcg atcgaatcc 
      ggagacgct gttttactc cgaaccgca ttagcatcc 
      aaacctgac gtaaacatc ctagatgaa aatgcaatg 
      acaccactg cagcatgct gcttataag ggcaacaag 
      gacatggtc caaatgctc ctcgactat ggggcagac 
      ccaaacctt tgcaaacac caacacaat tacacagcg 
      ctacatttc gcgggcctt tcgggcaat tccgacgtg 
      tgtctggca ctgctcgtg gctggggcc ttccccgaa 
      gtcacgaac gcagtgaat cggacggcg tcacaaatg 
      gcggccttt gtcggccat cataagtgc gtcgccatt 
      atcaataat tacatccca aagtcagaa gtagattat 
      tatagtagg ctacaaggc gctcaaacg gagccatat 
      ttgccgcca tttttgtcc gaaagcttc cacaaatta 
      ataatacaa gtaaacgta cacccggtc aaaatcgcg 
      ctaaacatc aacagtttt atcgggtta tcgcaacac 
      ttggcgaaa atcaaaaaa gtgctagaa ttgatgtac 
      gacaaagaa atgcaaaga ggggccggt aataacgaa 
      gtgatggcg ttcaaacta tactacctg gcgtttata 
      gtaggcgag gttatgacg attcaaaac aaacaccaa 
      tcgcctgac gccacgttg caaacgttt tgcaaaaaa 
      gtgctaaaa ccggctaca gatggcgct ttagaaata 
      ctcgatggg ttgttgcgg gagtgcatt aaacatttc 
      ccatttcgg gagtgtaca ctttttcgc caaatggtt 
      acaacacta acggggaaa gacacccca acagctcta 
      agtgttatc aattcggcc ataaatggt cagagaggg 
      ttcgttgat aacgtcccg atttgttac acttgtggg 
      gaagagaaa cctagtaag aagtgctcg caatgtaaa 
      gtggcgcag tattgtgat aagacgtgc caaaagttg 
      cactggtgt tggcacaaa aaggcgtgc ccacagttg 
      gctcaagag cccctcaaa tgtaataat gacacacaa 
      attgatcct caggaactg tcttcagaa atacagaat 
      cttttagtt actaattaa attgtaatt tattagtgt 
      gttagtaaa taaagtgag cttaacaaa
    • >TC004088 RB
      gaccttctt tctttgtca gagaaaaca catccaaat 
      tccaaagaa gtggaaaca acaaacgat tacaacgac 
      gagacaatg aaagatgaa gaagataga gcagttgaa 
      aactggaaa ggggaagtg acatttcat ctttccccg 
      attttgcca accgtcatt cctgtcaaa atttttgtt 
      ccatgttta tcacccgca aaaatctca acacaaagt 
      aaaaatgtc cacaagcac cgaaattga gaagcaaat 
      cttcactgc gatcgaatc cggagacgc tgttttact 
      ccgaaccgc attagcatc caaacctga cgtaaacat 
      cctagatga aaatgcaat gacaccact gcagcatgc 
      tgcttataa gggcaacaa ggacatggt ccaaatgct 
      cctcgacta tggggcaga cccaaacct ttgcaaaca 
      ccaacacaa ttacacagc gctacattt cgcgggcct 
      ttcgggcaa ttccgacgt gtgtctggc actgctcgt 
      ggctggggc cttccccga agtcacgaa cgcagtgaa 
      tcggacggc gtcacaaat ggcggcctt tgtcggcca 
      tcataagtg cgtcgccat tatcaataa ttacatccc 
      aaagtcaga agtagatta ttatagtag gctacaagg 
      cgctcaaac ggagccata tttgccgcc atttttgtc 
      cgaaagctt ccacaaatt aataataca agtaaacgt 
      acacccggt caaaatcgc gctaaacat caacagttt 
      tatcgggtt atcgcaaca cttggcgaa aatcaaaaa 
      agtgctaga attgatgta cgacaaaga aatgcaaag 
      aggggccgg taataacga agtgatggc gttcaaact 
      atactacct ggcgtttat agtaggcga ggttatgac 
      gattcaaaa caaacacca atcgcctga cgccacgtt 
      gcaaacgtt ttgcaaaaa agtgctaaa accggctac 
      agatggcgc tttagaaat actcgatgg gttgttgcg 
      ggagtgcat taaacattt cccatttcg ggagtgtac 
      actttttcg ccaaatggt tacaacact aacggggaa 
      agacacccc aacagctct aagtgttat caattcggc 
      cataaatgg tcagagagg gttcgttga taacgtccc 
      gatttgtta cacttgtgg ggaagagaa acctagtaa 
      gaagtgctc gcaatgtaa agtggcgca gtattgtga 
      taagacgtg ccaaaagtt gcactggtg ttggcacaa 
      aaaggcgtg cccacagtt ggctcaaga gcccctcaa 
      atgtaataa tgacacaca aattgatcc tcaggaact 
      gtcttcaga aatacagaa tcttttagt tactaatta 
      aattgtaat ttattagtg tgttagtaa ataaagtga 
      gcttaacaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY