| General information | |||
| TC004004 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 28614463..28652151 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004004 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00651 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC004004 RA
MGCLREQYL FNVFEAIIS TLESKLQRI ENLDKAVEH LMRRVEGLD NRVNNNIDK TESVIAKLS LLDSKLFNF DSKKPDGSN DGNVGRRLV ALDQKVSDI DNKLNVLKS QLDNNLLQG EDINAEASE KKPVSMNVV EITKALNTE VINHVTKEL GQLREATGS VDRKLQFHI TLVSENLGK VLTMMTDIH HAIVDDITS HYNRTTTPA PEPKNNKID ALVQQIRPI ISVSEKMDE VWDVVVGTK SSVDDLVPK SDELLTQTQ RQERAIGEI HNDLRTKTN KIIANLDKV EKRLKKQED DVATLAQRP VPAELLLDP TIDRLVEYD PSRYVDEYS TDTTLPATF TTLPPPTTA TQASTLNQS STTPVSSST QATTEKRAE KRKSGLIFP SVKNKPSPA NTTFATEVL ANVKDVKGY SCVDLLNAG MRDSGVYYL QIRGTTYWF LKVYCEQEI ADGGWTVIQ RRDDFGDPR ENFNRDWND YKNGFGDPA KEFWLGNEN IYMLTNNEE YILRVELED FEGNKRFAQ YSHFKIFSE GEYYKLEID GYEGNAGDS LNDPWYGSN NSPFSTYNR DNDRSSLNC ASMLKGGWW WKSCGRGLN GLYLNDPQD LTARQGIVW FRWRGWDYT LKKAMMMIK PKGPVTTT
- >TC004004 RA
atgggttgt ttaagagag caatacctc ttcaacgta ttcgaagcc ataatctca accctcgaa tccaaactc cagcggatc gaaaacttg gacaaagcc gtcgagcat ttgatgcga agggtggaa ggtttggac aatcgtgtt aacaacaac attgacaaa actgaatca gtgatcgca aaactgtcg ctccttgac tccaaactg ttcaatttc gattcgaaa aaaccagat gggtcaaat gatggcaac gtgggtcga cgattggtc gcccttgac cagaaagtg agtgacatt gacaataaa ctgaacgtt ttgaagagc cagctcgac aacaacttg ctccaaggc gaagacatc aatgctgaa gcaagcgag aagaaacca gtgagcatg aacgtggtg gaaatcacc aaggcgttg aacacggaa gtgataaac cacgtgacc aaagagttg ggccaattg agggaagcc acgggaagt gttgaccgg aaactgcaa tttcatatc actttagtc tcggagaat ttgggcaaa gtgttgact atgatgact gatattcac cacgccata gttgatgat attacaagt cattataat agaaccaca actccggcc ccggaaccc aaaaacaac aaaatcgac gctttggtg caacaaatc cgcccgatt atctccgtg tcggagaaa atggacgag gtttgggac gttgttgtt gggactaaa tcgtcggtt gacgatttg gtgccgaaa tctgacgaa ctgctgacg cagacgcag cgacaggag cgagcgatc ggcgaaatc cacaacgat ttgaggact aagaccaat aaaattatc gcgaatttg gataaggtt gagaagagg ttgaagaag caggaggat gatgtggcc acgttggcc cagagacct gtcccagct gagttgtta ttggatccg actattgat cgtttggtt gagtatgat cccagtcgg tatgttgat gagtattca actgatacg actcttccg gcgactttc accactttg ccgcccccg actaccgcc actcaagcg tcaacgttg aatcagagt agtacaact ccggtgagt tcttcaact caggccaca acagagaaa agggcggag aagaggaaa agtggcttg attttcccg agtgtgaaa aataagcct tcgccggcg aataccacg tttgcgacc gaagttttg gccaatgtc aaggatgtt aaggggtat tcttgcgtg gacctgctg aacgctgga atgcgtgat tccggtgtt tactacttg caaatccgg ggaaccacc tactggttc ttgaaggtg tattgcgag caagaaatc gccgatggt ggatggacg gtgatccag cgcagagac gacttcggc gaccctcga gagaacttc aacagggac tggaacgac tacaagaac ggcttcggc gacccggcc aaggagttc tggctcggc aacgaaaac atctacatg ctgaccaac aacgaggag tacatcctg cgcgtggag ctcgaggac tttgaagga aataaaaga tttgcccaa tactcgcac ttcaagata ttctcagaa ggggaatat tacaagttg gagatcgat ggttacgaa ggcaatgct ggggattcg ctgaacgat ccgtggtat ggaagcaac aacagtccg ttctcaaca tacaacaga gacaacgat aggtcgagc ctcaactgc gcctccatg ttgaagggc ggctggtgg tggaagtcc tgtggacgg ggccttaac ggcctctac ctcaacgat ccgcaagat cttacagca cggcaaggt atagtctgg ttccggtgg agaggctgg gactacacg ctgaagaag gccatgatg atgatcaaa ccgaaagga ccagttaca acaacataa
- >TC004004 RA
gtataaaaa aattaacct taataaaaa aacttattt cctcaaaaa agtaaataa ataaaaaat aaaaaatgg atacaatat ttaattttt acgaagata taaaaataa atgaaacat gggttgttt aagagagca atacctctt caacgtatt cgaagccat aatctcaac cctcgaatc caaactcca gcggatcga aaacttgga caaagccgt cgagcattt gatgcgaag ggtggaagg tttggacaa tcgtgttaa caacaacat tgacaaaac tgaatcagt gatcgcaaa actgtcgct ccttgactc caaactgtt caatttcga ttcgaaaaa accagatgg gtcaaatga tggcaacgt gggtcgacg attggtcgc ccttgacca gaaagtgag tgacattga caataaact gaacgtttt gaagagcca gctcgacaa caacttgct ccaaggcga agacatcaa tgctgaagc aagcgagaa gaaaccagt gagcatgaa cgtggtgga aatcaccaa ggcgttgaa cacggaagt gataaacca cgtgaccaa agagttggg ccaattgag ggaagccac gggaagtgt tgaccggaa actgcaatt tcatatcac tttagtctc ggagaattt gggcaaagt gttgactat gatgactga tattcacca cgccatagt tgatgatat tacaagtca ttataatag aaccacaac tccggcccc ggaacccaa aaacaacaa aatcgacgc tttggtgca acaaatccg cccgattat ctccgtgtc ggagaaaat ggacgaggt ttgggacgt tgttgttgg gactaaatc gtcggttga cgatttggt gccgaaatc tgacgaact gctgacgca gacgcagcg acaggagcg agcgatcgg cgaaatcca caacgattt gaggactaa gaccaataa aattatcgc gaatttgga taaggttga gaagaggtt gaagaagca ggaggatga tgtggccac gttggccca gagacctgt cccagctga gttgttatt ggatccgac tattgatcg tttggttga gtatgatcc cagtcggta tgttgatga gtattcaac tgatacgac tcttccggc gactttcac cactttgcc gcccccgac taccgccac tcaagcgtc aacgttgaa tcagagtag tacaactcc ggtgagttc ttcaactca ggccacaac agagaaaag ggcggagaa gaggaaaag tggcttgat tttcccgag tgtgaaaaa taagccttc gccggcgaa taccacgtt tgcgaccga agttttggc caatgtcaa ggatgttaa ggggtattc ttgcgtgga cctgctgaa cgctggaat gcgtgattc cggtgttta ctacttgca aatccgggg aaccaccta ctggttctt gaaggtgta ttgcgagca agaaatcgc cgatggtgg atggacggt gatccagcg cagagacga cttcggcga ccctcgaga gaacttcaa cagggactg gaacgacta caagaacgg cttcggcga cccggccaa ggagttctg gctcggcaa cgaaaacat ctacatgct gaccaacaa cgaggagta catcctgcg cgtggagct cgaggactt tgaaggaaa taaaagatt tgcccaata ctcgcactt caagatatt ctcagaagg ggaatatta caagttgga gatcgatgg ttacgaagg caatgctgg ggattcgct gaacgatcc gtggtatgg aagcaacaa cagtccgtt ctcaacata caacagaga caacgatag gtcgagcct caactgcgc ctccatgtt gaagggcgg ctggtggtg gaagtcctg tggacgggg ccttaacgg cctctacct caacgatcc gcaagatct tacagcacg gcaaggtat agtctggtt ccggtggag aggctggga ctacacgct gaagaaggc catgatgat gatcaaacc gaaaggacc agttacaac aacataaaa agtggaaga aaactagcc aatttataa tcaccggtc catttaatc ataaaattc atcaatcat aaatcaata tttatttcg acacatccg acacttacc tattttaaa ttacctcat agtatagta tactgaaaa tgatttttc acagaaata ttgcctaga acggttcta tcgaccaac gaattttcc ccagttgta tgtaaatat aaatgatag aattgttcc taatgtagg ttaagattg tacatttgc gctcccatc ttagttttg taaggaggt gtatttgca tgtagttac actaataca tacacagag acgcacaca catttcctg cacgatttt tgcaaaaac taacattta ctaatagcg aggcaaaat gtgtttaaa atttccgct gattataaa ttgggaaaa cacgaaaat gttgaggaa atttaagac tgtgtcgct tatcggagg aaagtttcg tcgataagt ttgtattaa tgtaaggta ttttgttgt gaggctaat gggacatga aatgcaatg ttcgtatta ggcaggtta aaatcaaat cttaccgta ggtcttgta ccttaagcg ttttacatg gttgttgta aatattcta tttcagagt tgtataaat taaatatat tttaatata ataaattat gttaagtat t
