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Gene information
General information
TC004000 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28793275..28798026
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004000
    iBeetle screen iB_00649

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004000 RA
      MALRIIGHL LKAPRHLLV RRYTSGGVG CGQQNKDDD 
      PCKAHLNKG LVLGIYTNP DDPLDPGKL TPTGERYNR 
      LVCNRLIDL LRYAGPPPT KGQVRIFYN LENEISSVA 
      VVGLGKECQ GYDVFEQMD EGKESIRIA AAAGCRALQ 
      DLRATKIFV ESFGHAESS AEGAALGVW LFQEKKQKE 
      HQLLIPQLE LWDDCDWTG WQIGLQKAA AQNLARQLM 
      DTSANYMTP TSFAQNAVE VLCKSGVNV EVKVRGWAE 
      TQKMFAFLA VAQGSCEPP IFLELSYYG AARDERPVV 
      LIGKGITYN SGGLCLKPC GKQEKMRGD MGGAACVVA 
      ACRAVAGLQ LPLNIRALI PLCENMPGC AAMRPGDIV 
      KAMNGKSIK IESTDTAGR LCLADALVY AQNFWPRLI 
      VDVGTMTSD MKKSLGKAA SGVFSNSEA LWEYMLAAS 
      MHTGDRVWR FPLWEHFTH LVARHHSAV DVKTYGRGF 
      KPRDGEACR VAAFLNEFV PCGDWLHMD TYGVMYSNG 
      LDYPYLRRG MSGRPTRTL VEFLSQLVC NRE
    CDS sequence
    • >TC004000 RA
      atggcactt cgcataatc gggcacctc ctcaaggcc 
      ccgcgccac ctcctggtg cgccgctac acgtcggga 
      ggcgtgggc tgcggccag cagaacaag gacgacgac 
      ccctgcaag gcccacctg aacaagggc ctcgtcctc 
      ggcatctac accaacccg gacgacccc ctggacccc 
      ggcaagctc acccccacc ggcgagcgc tacaaccgc 
      ctggtctgc aaccgcctc atcgacctc ctgcgctac 
      gccggcccc ccgcccacc aagggccaa gtgcgcatc 
      ttctacaac ctggagaac gagatcagc tcggtggcc 
      gtcgtcggc ctgggcaag gagtgccag ggctacgac 
      gtcttcgag cagatggac gagggcaag gagtccatc 
      cgcatagcg gcggccgcc ggctgccgc gccctccag 
      gacctgcgc gccaccaaa atcttcgtc gaaagcttc 
      ggacacgcg gagagctcc gccgaaggc gccgccctc 
      ggggtgtgg ctcttccag gagaagaag caaaaggag 
      caccaactc ttgatcccc cagctggag ctatgggac 
      gactgtgat tggacgggc tggcagatc gggctccag 
      aaagccgcc gcccagaac ctcgcccgc caattgatg 
      gacacctcg gccaactac atgacgccc acttcgttc 
      gcccaaaac gccgtcgag gttctctgc aaatcgggc 
      gtgaacgtc gaggtgaaa gtccgcggg tgggcggag 
      actcagaag atgttcgcc ttcctggca gtggcgcaa 
      ggctcatgc gaaccgccc attttcctc gagttgagc 
      tactacggg gcggcgcgt gacgagcgc cccgtcgtt 
      ctgattggc aaaggcatc acgtacaac agcggtggg 
      ctgtgcctg aagccgtgc ggcaagcag gagaagatg 
      cggggggac atgggcggg gccgcgtgc gtggtcgcc 
      gcatgccgg gcggtggcc ggcctccag ctccccctc 
      aacatccgt gccctcatc cccctctgc gagaacatg 
      cccgggtgc gcggccatg cggccgggg gacatcgtc 
      aaggccatg aacggcaaa agcatcaaa atcgagtcg 
      acggacacg gcggggcgg ctgtgtcta gccgacgcc 
      ctcgtctac gcccagaac ttctggcca cgcctcatc 
      gtggacgtg gggacgatg acgagcgac atgaagaag 
      tcgctgggg aaggcggcc agtggcgtt ttcagcaac 
      tcggaggcg ctgtgggag tacatgctg gcggcgtcg 
      atgcatacg ggggacagg gtgtggagg ttccccctg 
      tgggagcat tttacgcac ttggtggcg aggcaccat 
      tcggcagtt gatgtcaag acttatggg cggggcttc 
      aagccgagg gatggggag gcgtgccga gtggcggcg 
      tttttgaac gagtttgtc ccgtgtggc gattggctg 
      catatggat acctatggg gtgatgtac agcaacgga 
      ttggattat ccgtatttg aggcggggc atgagtgga 
      aggcccacg aggacgctt gtggagttc ttatcgcag 
      cttgtttgc aaccgtgaa taa
    mRNA sequence
    • >TC004000 RA
      aaggattcc aaaattttc aaaaaaact atacgattg 
      tgtttttcc gaaattctg gtcacttct gtcgtcaca 
      ttagcgtcg aaatattgt tttgccaaa aatcaaaat 
      aaattgtgt cgcttttta caaaagtga ttaatttca 
      ttttgcgaa ataaaggaa cctgtaatt ctattcagt 
      gttgccaat ttaactttt tcaggccag tctaaccaa 
      aaattagaa atttctggc ttgaaaatt ttgctgcac 
      agtttcgat tagaatttt acgatcaat tttcaaaaa 
      taggaattt ttagatgtg tacaaattt gtgtacatc 
      ggggaaaaa tcgacggtt agctcggtt tcaataagt 
      ccaataaat aaatacatt ggaaaaaaa aatggaaaa 
      acggtcgtt tcttcctac ttcaggcct aatttggaa 
      cttgttgaa aaatgttca aataaaggc caaacaaat 
      taatactca aattagatt gaaataacg taaaaattt 
      gaataaaaa ccgcgtctt acgttcaaa tttagctaa 
      agacaaata agaatctct aactaaaat tctagtttt 
      aagaaatct gtcaaaatt aattgcaaa aaatttcca 
      gttttttaa aataagcgg ttttggaat gtggtaaat 
      aacatgttt gacgtaaaa tgatccgga aaatccaaa 
      tttatgtaa atttcacgg aaaaattga agtgatttt 
      tgtgagaga tggccaaga atttaattt tcagaaaaa 
      ggtggttaa tagaaaaaa caatgttta tttgcaatt 
      taatgaacc tgaagatgg acatttgat tattgaccg 
      aaacatgta gcttgtgag taaaattat gtagtttca 
      attcgactg tggttttta tcactactt tttgtcatt 
      ttatcactg atttttctt atgtaaaag aaatttaaa 
      tttaaaacc gaaaaatct ggagtcaaa ttttttttc 
      taattttta aaacattcg tttaagaaa tataatgaa 
      tgacttttg ttttgtaaa ataaccgga aattttaag 
      atttgtata aatctatcc ggaaaaatt aaccaaata 
      atctcggaa cgtccgtaa ttcaaataa tatctataa 
      aatatacga aagttttaa gtaaatgga tttaaataa 
      tcagattat agccgaatt tcagcgaat tttattttt 
      tcttataat acaagaaat atgaaaagt ttatgagaa 
      tgagaaaaa ggcgaaatt acaataaaa tttcctatt 
      ttttaaatt acgctgttt gaaaacgtg tcaaatgac 
      atttcttta ttaagatga accaaggac tccgaaact 
      gtaaaaaat ttcagaaaa cataaacat attattata 
      attgagttt caggttaat tcaaatctt tttaaaaaa 
      atcatacaa actcctaaa aacttggaa atatttacg 
      cgtacttaa gtatttttt tataaccgg aaaaatccg 
      gagttaaaa aatattttc taatttttc aacatgcgt 
      tttagaaat gtacagtaa acgattttt cttatataa 
      aataggcaa taaattctt gtacaaaat ttattaaaa 
      atgaagtag tttcggaac ttcaagtga ataaattca 
      tacttaaaa cggaaattt aaaccaaaa aattcaaat 
      ttgtgaaaa ttttcgcca aaaatttaa tcaaataat 
      ccgggaatg aagagaatt ttacttaaa tttgtcaaa 
      tataaagtt ttacgtgga ttaattaaa aaaatccaa 
      ttataaccg gatttcacc gattttttt tataatttt 
      agtaatgat taaataatt aaataataa cgtaaacct 
      aaggaacac caaatgtga aaatttctc attttccga 
      aataacacg acaagttcc aggttaatt taaacccga 
      aacaacttt tgagtgatt tttattata tccgaaaaa 
      tttggagcg aaaaatatt ttacaattt ttaaatgat 
      gctgtttag aaataatat tgtattaaa taatttttc 
      ttaaataaa ataggccga aaaatgcag ttccagaaa 
      tcagatttt tgtaaaaat gtttgaaaa taaacactt 
      aaaaaaatt tgtaaagaa atttctttt tataattat 
      aaattttta taattataa taaaagctc aagtggggg 
      cttcaaata atccggatt atattcaaa tttcaattt 
      ttttttttg gaacaaata gtttatcag aattcatta 
      gtttatttt gtaaaaaac tagaactac gggatttga 
      aaatgtggc aaatgacaa tgaaccaaa aaattcgaa 
      gttgtaaaa attttccag ataaaataa acacttcta 
      gaaaaatcc tggaaaatt caaaacttt attgttgaa 
      gaacattaa aataaacgt taaaaacta acttaaatt 
      tttgtgctt attgaaacc gagatgact ctagtttaa 
      aaattaacc actgaaact gtataaatt agtattgtg 
      cgaagtttc aataggaaa gtcggtaaa aaaaatcag 
      tattatgca tattgtaga ggtagcaat actatacat 
      atcttaaat cgacttgag aagctattt tattattag 
      cctgtacaa accgtacga aacccgtgc agaaaacga 
      gaataataa taatgttaa accactgtt atttatgca 
      tgatgtatt aaaaaaaat ctcaataaa atccgttaa 
      ccaagccgc cgttttgat tttcccccc gctttcaat 
      cgccaacgt aaccaaata aacaaaaaa atcattcga 
      aaattttct gacacaaat cctcccaaa cccaaaaat 
      tgtgagtaa tgaagcaaa cctccgacc agtaagtaa 
      gaaaatggc acttcgcat aatcgggca cctcctcaa 
      ggccccgcg ccacctcct ggtgcgccg ctacacgtc 
      gggaggcgt gggctgcgg ccagcagaa caaggacga 
      cgacccctg caaggccca cctgaacaa gggcctcgt 
      cctcggcat ctacaccaa cccggacga ccccctgga 
      ccccggcaa gctcacccc caccggcga gcgctacaa 
      ccgcctggt ctgcaaccg cctcatcga cctcctgcg 
      ctacgccgg ccccccgcc caccaaggg ccaagtgcg 
      catcttcta caacctgga gaacgagat cagctcggt 
      ggccgtcgt cggcctggg caaggagtg ccagggcta 
      cgacgtctt cgagcagat ggacgaggg caaggagtc 
      catccgcat agcggcggc cgccggctg ccgcgccct 
      ccaggacct gcgcgccac caaaatctt cgtcgaaag 
      cttcggaca cgcggagag ctccgccga aggcgccgc 
      cctcggggt gtggctctt ccaggagaa gaagcaaaa 
      ggagcacca actcttgat cccccagct ggagctatg 
      ggacgactg tgattggac gggctggca gatcgggct 
      ccagaaagc cgccgccca gaacctcgc ccgccaatt 
      gatggacac ctcggccaa ctacatgac gcccacttc 
      gttcgccca aaacgccgt cgaggttct ctgcaaatc 
      gggcgtgaa cgtcgaggt gaaagtccg cgggtgggc 
      ggagactca gaagatgtt cgccttcct ggcagtggc 
      gcaaggctc atgcgaacc gcccatttt cctcgagtt 
      gagctacta cggggcggc gcgtgacga gcgccccgt 
      cgttctgat tggcaaagg catcacgta caacagcgg 
      tgggctgtg cctgaagcc gtgcggcaa gcaggagaa 
      gatgcgggg ggacatggg cggggccgc gtgcgtggt 
      cgccgcatg ccgggcggt ggccggcct ccagctccc 
      cctcaacat ccgtgccct catccccct ctgcgagaa 
      catgcccgg gtgcgcggc catgcggcc gggggacat 
      cgtcaaggc catgaacgg caaaagcat caaaatcga 
      gtcgacgga cacggcggg gcggctgtg tctagccga 
      cgccctcgt ctacgccca gaacttctg gccacgcct 
      catcgtgga cgtggggac gatgacgag cgacatgaa 
      gaagtcgct ggggaaggc ggccagtgg cgttttcag 
      caactcgga ggcgctgtg ggagtacat gctggcggc 
      gtcgatgca tacggggga cagggtgtg gaggttccc 
      cctgtggga gcattttac gcacttggt ggcgaggca 
      ccattcggc agttgatgt caagactta tgggcgggg 
      cttcaagcc gagggatgg ggaggcgtg ccgagtggc 
      ggcgttttt gaacgagtt tgtcccgtg tggcgattg 
      gctgcatat ggataccta tggggtgat gtacagcaa 
      cggattgga ttatccgta tttgaggcg gggcatgag 
      tggaaggcc cacgaggac gcttgtgga gttcttatc 
      gcagcttgt ttgcaaccg tgaataaat tttcaatgt 
      atgatattt acgagattt tttgttttt ttatttggt 
      atatgaact gcttcaact cacctgtaa tgtaattta 
      caataaacc attaaagat att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY