Status
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Gene information
General information
TC003986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 29128387..29174874
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003986
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00647

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    vitellarium not present - Number of animals showing the phenotype: 3
    egg chamber not present - Number of animals showing the phenotype: 3
    tropharium size decreased - Number of animals showing the phenotype: 3
    tropharium shape rounded - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    remarks: later than schedule, larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003986 RA
      MGIRHLLVL FVVVLLVLV VCDCRQHYN RNRRRGGAK 
      DKALALHHQ ENEFHNKGK ICIGTNGRM SVPSNREHH 
      YRNLKDRYT NCTYVDGNL ELTWLQDEN LDLSFLQYI 
      REVTGYVLI SHVDIKRIV LPRLQIIRG RTLFKMNVR 
      NEEFALLVI LSKMYTLEL PALRDVLIG NVGVFNNYN 
      LCHFKTINW KEIITDPKS KYVFVYNFT SPERDCPPC 
      HKNCEKGCW GEGEENCQK FSKENCSPQ CYQGRCFGP 
      NPRECCHLF CAGGCTGPK QSDCIACRN FYDDGVCTQ 
      ECPPMKIYS PITYSWQDN PNGKYAYGA TCVKNCPEH 
      LLKDNGACV RSCPPDKKA HEGACVPCN GPCPKTCRV 
      DTFIHSGNI DTFKGCTVI EGNILILQN TFEGYQHFY 
      PNYTFGARY PRMHPDRLE VFSTLKEVT GHINVQAYH 
      SDFTNLSYF RNLEVIGGR SLSDYFTSL YIVKSSLKS 
      LELRSLKRL NAGTVAILE NTHLCFADN INWDKIRRS 
      HEHVVMISN NSDPRLCKG NNLVCDSEC SRDGCWGAG 
      PDQCLSCAH FQLENESRC IANCSVLPG IYQDGPNIC 
      KRCHEECGS SCTGPGADK CVTCKRFQD GPYCVPSCP 
      TNKYHENGQ CKSCHANCV GGCTGPTNI VGLGGCNSC 
      DRAIMNETD SMVYCLGSN ESCPDGYFN EYLGPQEKE 
      SLKSLAGSA LCRKCHPRC KICSGFGFH ELVCQKCTH 
      YKKDEQCED ECRSGDYAD EEVQECKPC DPECKGCTG 
      PGPENCISC ENFKVFDDG YVSENSSFR CVSSCPPEF 
      PHRVFPDNA FSYCSDKPL NLPISGFVD NNYTVVIAT 
      GITSAIIII IIIIILVTW FFRKKVKAE ESTLKMTMA 
      LTGLEDNEP LRQTNVSPN CKKLRTIKE TEIRRGDIL 
      GYGAFGTVY KGVWILEDE NNSKIPVAI KVLRDGSTS 
      SKEFLSEAF IMASVEHPN LLQLLGVCM TSEIMLITQ 
      LMPLGCLLD FVRKNRDRI GAKALLNWS TQIAKGMAY 
      LEEKRLVHR DLAARNVLV QTHSCVKIT DFGLAKLLD 
      VDEDEYKAA GGKMPIKWL ALECIQHRI FTHKSDVWA 
      FGVTIWELL TFGERPYDN VPARDVPEL LEKGERLPQ 
      PEGCSIDVY MILVKCWIL DAESRPSFK ELAEDFTKM 
      ARDPCRYLA ITADRFARN RNNAQDDRK LVHTLASTL 
      ETSASQSEC DEYLQPKSR APLRPVGWM SSVSPSPTP 
      DKYWKSASQ TNSDGSCNP QYQNHLNRK LLKYPPSTA 
      SDTLKMRDD MSSDEYDSG PSKAQLGSL KLDLPVDED 
      DYLMPSPQQ TQGASAYVD LIDSKNSDT SNQSVFRSL 
      DFYKSNIDN PEYLMNNEA APSQTVGLP QVPRSSEEE 
      SDHEYYNDF DRLKRELQP LQRNKDGAI V
    CDS sequence
    • >TC003986 RA
      atggggata cgccacctc ctggtgttg ttcgttgtg 
      gtgttgttg gttttggtg gtgtgcgat tgtagacag 
      cactacaac aggaacaga cgcagaggc ggcgccaag 
      gataaggcg ctggctctc catcaccaa gagaatgag 
      ttccacaac aagggaaag atttgcatt ggaacaaac 
      ggccgcatg tccgtcccc tcaaaccgc gagcaccac 
      taccgcaac ctgaaggac cgctacacc aactgcacc 
      tacgtggac ggcaacctg gagctgacc tggctccag 
      gacgagaac ctggacttg agcttcctc caatacatc 
      cgtgaagta acgggttac gtcctcatc tcgcacgtg 
      gacatcaag cgcatcgtc ttgccccgc ctccaaatc 
      atccgcggt cgcaccctc ttcaagatg aacgtccgc 
      aacgaggag ttcgccctc ttggtcatc ctctccaag 
      atgtacacc ttggagttg cccgccctc cgggatgtc 
      ctcatcgga aacgttgga gttttcaac aattacaac 
      ctttgccac ttcaagacc atcaactgg aaggaaatc 
      atcacggac cccaagagc aagtacgtc ttcgtctac 
      aacttcacc tcccccgaa cgcgactgt cccccatgt 
      cacaagaac tgtgagaaa gggtgttgg ggggaaggc 
      gaagaaaac tgccagaag ttttccaag gagaactgc 
      agtccacag tgttaccaa gggcggtgt ttcgggccg 
      aacccacgg gagtgctgc cacttgttt tgtgcggga 
      ggttgtacc ggacctaag caaagtgat tgtatcgcc 
      tgtcggaac ttttacgac gatggggtt tgcacacag 
      gagtgcccc cctatgaaa atttacagc cccattacg 
      tactcctgg caggataac cctaacggg aagtacgct 
      tacggtgcc acgtgtgtg aaaaactgc cctgaacat 
      ttactcaaa gacaatggg gcttgcgtc cggtcctgc 
      ccccctgac aagaaggcc catgaaggg gcgtgtgtc 
      ccttgcaac ggcccgtgc cccaaaacc tgccgcgtt 
      gatactttc atccattct gggaacatt gacaccttc 
      aagggttgt actgtgata gaaggcaat atcctgatt 
      ttgcaaaac accttcgag gggtaccaa cacttctac 
      cccaactac accttcggg gccaggtac ccccggatg 
      caccccgac cgcttggag gtctttagc accctgaag 
      gaggtcacg gggcatatc aacgttcaa gcgtaccat 
      tccgatttt accaatttg tcgtacttt agaaacttg 
      gaagtgatc ggtggtcgc tcactttca gactacttc 
      acttcgctg tatattgtt aaatcgtcg ctgaaatcg 
      ctcgagttg cggtcgttg aagcgactc aatgccggg 
      acggtcgcc attttggaa aacacacac ttgtgtttt 
      gctgataat atcaattgg gataaaatc aggaggagt 
      cacgagcat gtggttatg atttcgaat aatagtgat 
      ccacggttg tgcaagggg aacaatttg gtgtgtgat 
      agtgagtgt agtagggat gggtgttgg ggggctgga 
      cccgatcag tgtttgtcg tgtgcccac ttccagctc 
      gagaatgag agtcggtgt attgccaac tgctccgtt 
      ttgccaggg atttaccaa gacggtccc aacatttgc 
      aagcgctgc cacgaagaa tgcggctct tcgtgcaca 
      ggtccaggt gccgacaaa tgcgtcact tgcaagcgc 
      ttccaggat ggtccctac tgcgtacca tcctgtccc 
      actaacaag taccacgaa aacggtcag tgcaagagc 
      tgccacgcc aattgcgtc gggggatgc accggtccc 
      accaacatt gttggttta ggcggctgt aactcgtgc 
      gaccgtgct atcatgaac gaaaccgac tccatggtc 
      tattgcttg ggcagcaac gagtcctgt cctgacggc 
      tacttcaac gagtacttg gggccgcag gagaaggag 
      tcgctcaaa tcgctggcg ggctcagcg ctgtgccgg 
      aaatgccat ccaagatgc aagatctgt tccggcttc 
      ggtttccac gaactagtc tgccaaaaa tgcacccac 
      tacaagaaa gacgagcaa tgcgaagac gaatgtcgt 
      tccggtgac tacgcagac gaagaagtc caagaatgc 
      aaaccttgc gaccctgaa tgtaaaggc tgcactggt 
      ccaggtccc gaaaactgt atttcctgc gaaaatttt 
      aaagttttc gacgacgga tacgtttcc gaaaactct 
      tccttccgc tgcgtgtcc tcatgccca cctgaattc 
      ccacaccgg gttttcccc gacaatgcc ttctcctac 
      tgttccgac aaacccttg aacttgccc atctctggt 
      ttcgtcgac aataactac actgtggtg atcgccacg 
      ggaatcact agtgcgatc atcatcatc atcataatc 
      ataattttg gtgacttgg ttttttcgc aagaaggtc 
      aaggcggaa gagagcacc ttgaagatg accatggcg 
      ttgactggg ctcgaagac aacgaaccg ttgcgacaa 
      acgaacgtt tcgcccaat tgtaaaaaa ttgcgcacg 
      attaaggaa acggaaatc aggcggggg gatattttg 
      gggtatggc gccttcgga actgtctat aaaggggtc 
      tggattttg gaagatgag aacaattcg aaaatccca 
      gtcgcgatt aaagtcctc cgggacgga tcaaccagt 
      agcaaagaa tttttaagc gaggcgttt attatggca 
      agtgtggaa caccctaat ctccttcag ttgttgggt 
      gtttgtatg acttccgag atcatgctc ataacccag 
      ttgatgcct ttgggttgt ttgctcgat ttcgtccga 
      aaaaaccgc gaccgcatc ggcgctaaa gcccttctc 
      aactggtcc acgcaaatc gccaaagga atggcatac 
      ctggaggaa aaacgccta gtccaccgt gacctcgcc 
      gctcgcaac gtcctagtc caaacccac tcctgtgtc 
      aaaatcacc gacttcggc cttgccaag ctcctcgat 
      gtggacgaa gacgagtac aaagcagct ggtggtaaa 
      atgcccatc aaatggctg gccttggag tgcatccag 
      caccggatt ttcacgcac aagtcggac gtttgggcc 
      ttcggagtg accatttgg gagttgttg acttttggc 
      gaacgcccg tatgataat gtgccggcc agggacgtg 
      ccagagctg cttgagaag ggggagagg ttgccacag 
      cccgagggt tgtagtatc gatgtttac atgatcttg 
      gtcaagtgc tggattttg gacgccgaa agtaggccc 
      agttttaag gagttggcg gaggatttt accaaaatg 
      gcgagggat ccgtgccgg tatttggct attacggcc 
      gataggttc gccaggaat aggaacaac gcacaggat 
      gaccgtaag ctggtgcac acgcttgcc tccaccctc 
      gaaacctcg gccagccaa tcggagtgc gacgagtac 
      ctccagccc aagtcacgt gcgcctttg cgccccgtt 
      ggttggatg tcctccgtg tcgccctcc cccaccccc 
      gacaagtac tggaagtcg gcgtcgcag acaaattcg 
      gacggcagc tgcaacccg caataccag aaccacttg 
      aaccgcaag ttactcaag taccctcca agcaccgct 
      agcgacact ttgaagatg cgtgacgac atgtcttcc 
      gatgagtac gactccggt ccgtccaaa gctcagctg 
      ggaagtctc aaattggac ttaccggtg gacgaggac 
      gactatctc atgccgtcg ccgcagcag acgcaaggc 
      gcgtctgcc tacgtcgac ttgatcgac tcgaaaaac 
      agcgacact tcaaaccag agcgtgttc aggtctttg 
      gacttctac aagagcaat attgacaat cctgagtac 
      cttatgaac aacgaggcg gcgccgagt cagacggtg 
      ggcttgccc caggtgccg aggagctcc gaggaggag 
      tccgatcat gaatattac aatgacttc gaccgcctg 
      aagagggag ctgcagccg ctgcagcgg aacaaagac 
      ggcgcgatc gtctga
    mRNA sequence
    • >TC003986 RA
      ctaagaaac cggagatca gttcgttcg ttgtgcgca 
      catttttcg aattttcgc tgtgtgatg ttcgtttcg 
      gtgtctttg gtggtgcat cccagttgg ccgtgtttg 
      taggggtgg gggtagaag atggaagag agagtcagt 
      ttgttgttt ggcgcgagc tggtgagga gcttgcgcc 
      atgtgaggg acgaaaccg tcgacggcc atcatgggg 
      atacgccac ctcctggtg ttgttcgtt gtggtgttg 
      ttggttttg gtggtgtgc gattgtaga cagcactac 
      aacaggaac agacgcaga ggcggcgcc aaggataag 
      gcgctggct ctccatcac caagagaat gagttccac 
      aacaaggga aagatttgc attggaaca aacggccgc 
      atgtccgtc ccctcaaac cgcgagcac cactaccgc 
      aacctgaag gaccgctac accaactgc acctacgtg 
      gacggcaac ctggagctg acctggctc caggacgag 
      aacctggac ttgagcttc ctccaatac atccgtgaa 
      gtaacgggt tacgtcctc atctcgcac gtggacatc 
      aagcgcatc gtcttgccc cgcctccaa atcatccgc 
      ggtcgcacc ctcttcaag atgaacgtc cgcaacgag 
      gagttcgcc ctcttggtc atcctctcc aagatgtac 
      accttggag ttgcccgcc ctccgggat gtcctcatc 
      ggaaacgtt ggagttttc aacaattac aacctttgc 
      cacttcaag accatcaac tggaaggaa atcatcacg 
      gaccccaag agcaagtac gtcttcgtc tacaacttc 
      acctccccc gaacgcgac tgtccccca tgtcacaag 
      aactgtgag aaagggtgt tggggggaa ggcgaagaa 
      aactgccag aagttttcc aaggagaac tgcagtcca 
      cagtgttac caagggcgg tgtttcggg ccgaaccca 
      cgggagtgc tgccacttg ttttgtgcg ggaggttgt 
      accggacct aagcaaagt gattgtatc gcctgtcgg 
      aacttttac gacgatggg gtttgcaca caggagtgc 
      ccccctatg aaaatttac agccccatt acgtactcc 
      tggcaggat aaccctaac gggaagtac gcttacggt 
      gccacgtgt gtgaaaaac tgccctgaa catttactc 
      aaagacaat ggggcttgc gtccggtcc tgcccccct 
      gacaagaag gcccatgaa ggggcgtgt gtcccttgc 
      aacggcccg tgccccaaa acctgccgc gttgatact 
      ttcatccat tctgggaac attgacacc ttcaagggt 
      tgtactgtg atagaaggc aatatcctg attttgcaa 
      aacaccttc gaggggtac caacacttc taccccaac 
      tacaccttc ggggccagg tacccccgg atgcacccc 
      gaccgcttg gaggtcttt agcaccctg aaggaggtc 
      acggggcat atcaacgtt caagcgtac cattccgat 
      tttaccaat ttgtcgtac tttagaaac ttggaagtg 
      atcggtggt cgctcactt tcagactac ttcacttcg 
      ctgtatatt gttaaatcg tcgctgaaa tcgctcgag 
      ttgcggtcg ttgaagcga ctcaatgcc gggacggtc 
      gccattttg gaaaacaca cacttgtgt tttgctgat 
      aatatcaat tgggataaa atcaggagg agtcacgag 
      catgtggtt atgatttcg aataatagt gatccacgg 
      ttgtgcaag gggaacaat ttggtgtgt gatagtgag 
      tgtagtagg gatgggtgt tggggggct ggacccgat 
      cagtgtttg tcgtgtgcc cacttccag ctcgagaat 
      gagagtcgg tgtattgcc aactgctcc gttttgcca 
      gggatttac caagacggt cccaacatt tgcaagcgc 
      tgccacgaa gaatgcggc tcttcgtgc acaggtcca 
      ggtgccgac aaatgcgtc acttgcaag cgcttccag 
      gatggtccc tactgcgta ccatcctgt cccactaac 
      aagtaccac gaaaacggt cagtgcaag agctgccac 
      gccaattgc gtcggggga tgcaccggt cccaccaac 
      attgttggt ttaggcggc tgtaactcg tgcgaccgt 
      gctatcatg aacgaaacc gactccatg gtctattgc 
      ttgggcagc aacgagtcc tgtcctgac ggctacttc 
      aacgagtac ttggggccg caggagaag gagtcgctc 
      aaatcgctg gcgggctca gcgctgtgc cggaaatgc 
      catccaaga tgcaagatc tgttccggc ttcggtttc 
      cacgaacta gtctgccaa aaatgcacc cactacaag 
      aaagacgag caatgcgaa gacgaatgt cgttccggt 
      gactacgca gacgaagaa gtccaagaa tgcaaacct 
      tgcgaccct gaatgtaaa ggctgcact ggtccaggt 
      cccgaaaac tgtatttcc tgcgaaaat tttaaagtt 
      ttcgacgac ggatacgtt tccgaaaac tcttccttc 
      cgctgcgtg tcctcatgc ccacctgaa ttcccacac 
      cgggttttc cccgacaat gccttctcc tactgttcc 
      gacaaaccc ttgaacttg cccatctct ggtttcgtc 
      gacaataac tacactgtg gtgatcgcc acgggaatc 
      actagtgcg atcatcatc atcatcata atcataatt 
      ttggtgact tggtttttt cgcaagaag gtcaaggcg 
      gaagagagc accttgaag atgaccatg gcgttgact 
      gggctcgaa gacaacgaa ccgttgcga caaacgaac 
      gtttcgccc aattgtaaa aaattgcgc acgattaag 
      gaaacggaa atcaggcgg ggggatatt ttggggtat 
      ggcgccttc ggaactgtc tataaaggg gtctggatt 
      ttggaagat gagaacaat tcgaaaatc ccagtcgcg 
      attaaagtc ctccgggac ggatcaacc agtagcaaa 
      gaattttta agcgaggcg tttattatg gcaagtgtg 
      gaacaccct aatctcctt cagttgttg ggtgtttgt 
      atgacttcc gagatcatg ctcataacc cagttgatg 
      cctttgggt tgtttgctc gatttcgtc cgaaaaaac 
      cgcgaccgc atcggcgct aaagccctt ctcaactgg 
      tccacgcaa atcgccaaa ggaatggca tacctggag 
      gaaaaacgc ctagtccac cgtgacctc gccgctcgc 
      aacgtccta gtccaaacc cactcctgt gtcaaaatc 
      accgacttc ggccttgcc aagctcctc gatgtggac 
      gaagacgag tacaaagca gctggtggt aaaatgccc 
      atcaaatgg ctggccttg gagtgcatc cagcaccgg 
      attttcacg cacaagtcg gacgtttgg gccttcgga 
      gtgaccatt tgggagttg ttgactttt ggcgaacgc 
      ccgtatgat aatgtgccg gccagggac gtgccagag 
      ctgcttgag aagggggag aggttgcca cagcccgag 
      ggttgtagt atcgatgtt tacatgatc ttggtcaag 
      tgctggatt ttggacgcc gaaagtagg cccagtttt 
      aaggagttg gcggaggat tttaccaaa atggcgagg 
      gatccgtgc cggtatttg gctattacg gccgatagg 
      ttcgccagg aataggaac aacgcacag gatgaccgt 
      aagctggtg cacacgctt gcctccacc ctcgaaacc 
      tcggccagc caatcggag tgcgacgag tacctccag 
      cccaagtca cgtgcgcct ttgcgcccc gttggttgg 
      atgtcctcc gtgtcgccc tcccccacc cccgacaag 
      tactggaag tcggcgtcg cagacaaat tcggacggc 
      agctgcaac ccgcaatac cagaaccac ttgaaccgc 
      aagttactc aagtaccct ccaagcacc gctagcgac 
      actttgaag atgcgtgac gacatgtct tccgatgag 
      tacgactcc ggtccgtcc aaagctcag ctgggaagt 
      ctcaaattg gacttaccg gtggacgag gacgactat 
      ctcatgccg tcgccgcag cagacgcaa ggcgcgtct 
      gcctacgtc gacttgatc gactcgaaa aacagcgac 
      acttcaaac cagagcgtg ttcaggtct ttggacttc 
      tacaagagc aatattgac aatcctgag taccttatg 
      aacaacgag gcggcgccg agtcagacg gtgggcttg 
      ccccaggtg ccgaggagc tccgaggag gagtccgat 
      catgaatat tacaatgac ttcgaccgc ctgaagagg 
      gagctgcag ccgctgcag cggaacaaa gacggcgcg 
      atcgtctga acgcatgta caaaacggc cttttgtac 
      tttagaggg aaagggctg tgttccact aggagatac 
      ttaagcgta ggtttaagc gtgacataa tagcgtgcc 
      atcagggca tggtttgta aataatgtt gcaacaaat 
      gcgattttt attctcaaa tgtatataa atacctaaa 
      cgtattttt ctagtaggt tttaaggtt gattaacaa 
      gttgggcga gttgcggag cgccaatcc taaacgttc 
      ttgcccaaa cgcgactgt gataaaaac gaacttaca 
      tgtgaatat ggaaaaagt ttcatacac ttaaacccc 
      ttcatgcat tttgtaaac ccagttttt ttgttttgt 
      tttttgttt gtatggtat taaagcaat tcctctagt 
      tattagtta tggaattag aaagcttga aacgttcgg 
      tctcatgca aaatctcaa ttgtacata attttattt 
      atgtagaaa tagactatt tttgttaat atttgcact 
      aaacttgtg ccattcatg tcgacatgt gcctatgtt 
      atttttcca tgttactac gtttataca aaattttaa 
      taaatgcat atatttcat
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