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Gene information
General information
TC003971 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid29: 1277066..1329820
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003971
    iBeetle screen iB_00644
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 remarks: bad quality of preparation,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003971 RA
      MSTAVTNGH QAPSEDFAK HVFGAHERG HELLMGFTF 
      TLESILSQQ RLVSVILMM ILLAAELVE EDTKMCGKI 
      LTVLSWMIV VLTMPFSLF VCFKVVQEY ERAVIFRLG 
      RLLSGGAKG PGIFFILPC IDAYARVDL RTRTYDIPP 
      QEVLTKDSV TVSVDAVVY YRVSNATVS IANVENAHH 
      STRLLAQTT LRNIMGQRP LHEILSERE SISQHMKAL 
      LDEATDSWG INVERVEIK DVRLPIQLQ RAMAAEAEA 
      AREARAKVI AAEGEQKAS RALREASEV IGDSPAALQ 
      LRYLQTLNT ISAEKNSTI VFPLPIDML TYFLKAQEA 
      SSS
    • >TC003971 RB
      MSTAVTNGH QAPSEDFAK HVFGAHERG HELLMGFTF 
      TLESILSQQ RLVSVILMM ILLAAELVE EDTKMCGKI 
      LTVLSWMIV VLTMPFSLF VCFKVVQEY ERAVIFRLG 
      RLLSGGAKG PGIFFILPC IDAYARVDL RTRTYDIPP 
      QEVLTKDSV TVSVDAVVY YRVSNATVS IANVENAHH 
      STRLLAQTT LRNIMGQRP LHEILSERE SISQHMKAL 
      LDEATDSWG INVERVEIK DVRLPIQLQ RAMAAEAEA 
      AREARAKVI AAEGEQKAS RALREASEV IGDSPAALQ 
      LRYLQHGSL GRFSRKRGA RG
    CDS sequence
    • >TC003971 RA
      atgtcaaca gctgtaaca aatggacac caggcccct 
      tcggaggat tttgccaaa catgtattc ggagcgcat 
      gaacgtgga cacgaattg ttgatgggc ttcaccttc 
      actctcgag tcaatttta tcgcaacaa cgattggtt 
      tctgttatt ttgatgatg attttgttg gcagctgag 
      ctggtggag gaggacacg aaaatgtgc ggcaaaatc 
      ctaacagtc ctgtcctgg atgatcgtc gttttgacg 
      atgcccttc tccctcttc gtctgcttt aaggtcgtt 
      caagagtac gaaagggcc gtcattttt cgcttaggt 
      cgtctcttg tccggaggc gccaaaggg ccaggaatt 
      tttttcatc ttgccgtgt attgatgcc tacgccagg 
      gttgacctg cggaccagg acctacgac atccctcca 
      caagaggtt ctgaccaaa gacagcgtg accgtctcc 
      gtggacgcc gtggtctac taccgcgta tccaacgcc 
      accgtctcc atagccaac gtggagaac gctcaccac 
      tcgactcgc ctcctcgcc cagaccacc ctccgcaac 
      atcatgggc caacgtccc ctccacgag atcctcagc 
      gagcgcgag agcatctcc cagcacatg aaggccctc 
      ctcgacgag gccaccgac tcctggggc atcaacgtg 
      gaacgagtt gaaatcaaa gacgtgcga ctgcccatc 
      cagctgcag agggccatg gcagctgaa gctgaagct 
      gctcgtgag gcccgtgct aaagtcatc gccgccgag 
      ggcgagcag aaggcctcc agggccttg cgagaggcc 
      tcggaggtg attggggat tccccggct gcgctccag 
      ctcagatac ttgcagacg cttaacacg atctcagct 
      gagaagaac tcgacgatt gtgtttccg ctgcctatt 
      gatatgttg acgtacttc ttgaaggct caggaggcc 
      tcctcttcg taa
    • >TC003971 RB
      atgtcaaca gctgtaaca aatggacac caggcccct 
      tcggaggat tttgccaaa catgtattc ggagcgcat 
      gaacgtgga cacgaattg ttgatgggc ttcaccttc 
      actctcgag tcaatttta tcgcaacaa cgattggtt 
      tctgttatt ttgatgatg attttgttg gcagctgag 
      ctggtggag gaggacacg aaaatgtgc ggcaaaatc 
      ctaacagtc ctgtcctgg atgatcgtc gttttgacg 
      atgcccttc tccctcttc gtctgcttt aaggtcgtt 
      caagagtac gaaagggcc gtcattttt cgcttaggt 
      cgtctcttg tccggaggc gccaaaggg ccaggaatt 
      tttttcatc ttgccgtgt attgatgcc tacgccagg 
      gttgacctg cggaccagg acctacgac atccctcca 
      caagaggtt ctgaccaaa gacagcgtg accgtctcc 
      gtggacgcc gtggtctac taccgcgta tccaacgcc 
      accgtctcc atagccaac gtggagaac gctcaccac 
      tcgactcgc ctcctcgcc cagaccacc ctccgcaac 
      atcatgggc caacgtccc ctccacgag atcctcagc 
      gagcgcgag agcatctcc cagcacatg aaggccctc 
      ctcgacgag gccaccgac tcctggggc atcaacgtg 
      gaacgagtt gaaatcaaa gacgtgcga ctgcccatc 
      cagctgcag agggccatg gcagctgaa gctgaagct 
      gctcgtgag gcccgtgct aaagtcatc gccgccgag 
      ggcgagcag aaggcctcc agggccttg cgagaggcc 
      tcggaggtg attggggat tccccggct gcgctccag 
      ctcagatac ttgcagcat ggctcactt gggcgcttt 
      tctcgcaaa cggggggct aggggttaa
    mRNA sequence
    • >TC003971 RA
      acggtcctg aaaagcgca tttttcacg ctttaagcc 
      cttgtgtgt ttcctgaaa ccgcttttt tcaggaata 
      gtttcagtt gcatgtcaa gcgtgcgtt tgcgctcta 
      gtctttcaa gaaacagtg gcaaataca agtccggca 
      acgctcatc acattgaca catcaaagt gcgacgtta 
      tccttcagg agtcctgac gcccacttc gatgccaac 
      atcctgaaa acaatacaa aaattgcga ctctactca 
      ttgtcaaac cctgcaaca aggtggcgg caaccgacg 
      tgaaagtag cccctgata tgtcaacag ctgtaacaa 
      atggacacc aggcccctt cggaggatt ttgccaaac 
      atgtattcg gagcgcatg aacgtggac acgaattgt 
      tgatgggct tcaccttca ctctcgagt caattttat 
      cgcaacaac gattggttt ctgttattt tgatgatga 
      ttttgttgg cagctgagc tggtggagg aggacacga 
      aaatgtgcg gcaaaatcc taacagtcc tgtcctgga 
      tgatcgtcg ttttgacga tgcccttct ccctcttcg 
      tctgcttta aggtcgttc aagagtacg aaagggccg 
      tcatttttc gcttaggtc gtctcttgt ccggaggcg 
      ccaaagggc caggaattt ttttcatct tgccgtgta 
      ttgatgcct acgccaggg ttgacctgc ggaccagga 
      cctacgaca tccctccac aagaggttc tgaccaaag 
      acagcgtga ccgtctccg tggacgccg tggtctact 
      accgcgtat ccaacgcca ccgtctcca tagccaacg 
      tggagaacg ctcaccact cgactcgcc tcctcgccc 
      agaccaccc tccgcaaca tcatgggcc aacgtcccc 
      tccacgaga tcctcagcg agcgcgaga gcatctccc 
      agcacatga aggccctcc tcgacgagg ccaccgact 
      cctggggca tcaacgtgg aacgagttg aaatcaaag 
      acgtgcgac tgcccatcc agctgcaga gggccatgg 
      cagctgaag ctgaagctg ctcgtgagg cccgtgcta 
      aagtcatcg ccgccgagg gcgagcaga aggcctcca 
      gggccttgc gagaggcct cggaggtga ttggggatt 
      ccccggctg cgctccagc tcagatact tgcagacgc 
      ttaacacga tctcagctg agaagaact cgacgattg 
      tgtttccgc tgcctattg atatgttga cgtacttct 
      tgaaggctc aggaggcct cctcttcgt aatttcgtc 
      gagggatga tttttgcag atactcata cacgacgta 
      agttcaagt aatcacgtt ttcgggcac agtttacca 
      acctagttg ttgttttcg atgttctta ttgtataaa 
      aatgtacta taacgaagt taaataaag ttctattta 
      ctactttac ttccacgtt catttcgta cgcttcgat 
      ctaggatat aatcttcct tgtcccact gattagtgt 
      cacgtctta atgtctttg gtttttgtc agactttga 
      actcgatat cgacgggaa aaaattcga caattgtgt 
      tccccatcc cgcttgata ttgtctcat attttctca 
      agttcgaag aaggaatgg agaattttc taaaataga 
      gaggagtaa atgtgtcct tgcaccaac ggtgtcctt 
      gaaacctga cgatgtaaa tttatgttc aagcacggt 
      gttcacact atgatatgg ttgtgctag aattagtgc 
      caatattcg catgttagt tcatagtaa atgtaaacg 
      cactgtagg gatttgaaa ggtccgaga actaaaaag 
      tacacagtt caatgccaa aagtagtgc caaatttat 
      cactgtggt gcgataaat caaaagtgg agattacag 
      ggtcattga tagtgtgtc tctttagga actggttta 
      cataaatta atcaaacat attaaataa ggttgtgcc 
      taaaacgaa agttgattt ttattttag ttttgtaca 
      aaattggga acaaaatgc acaataatt acgtttttg 
      actttagtt taattatga tgtatttca taatcaaag 
      gagtgttag ttattgtta catagttaa aaaatgttg 
      gttataaaa atatttacc gttataagt gtgttaaaa 
      acaaatgaa tttaaaata gtctagtta aaccaagtg 
      gttgtatca tctgcagca agtgagttg gaatttttt 
      tgtttctgc ttttttcat cagaaaaat acaaaatac 
      gtcttctca aatcaagaa tgctagact aggtcggat 
      caggtcagg tcaggtcag gtcaggtta cctaaagtc 
      aaaagtgtt ttttttgga aaaataaaa aaacctggc 
      aaaaggctt agtggttct attttctag ttgagatat 
      actgttttt acaaagcca atgacatgt cttaaatag 
      accttgact aagtcaggt ctggtctaa gtacttttt 
      aagataaat tcagtttaa tatgtttta tattagaaa 
      ttttaatac agaaagttt tttgttttt gattttttc 
      gtaatttga ggaaaatac aaaatacgt tttttcaaa 
      tcaagaatg ctaaaccag gtttggtca ggtcaggtc 
      agatcttga ctgtataaa ctaagtaag tgtaagcaa 
      ttttataaa aaaaatttt ttaataatt ctaatattt 
      tacagttta tttttctct cagtcgttt ctctaagaa 
      aaaaacgaa aagagt
    • >TC003971 RB
      acggtcctg aaaagcgca tttttcacg ctttaagcc 
      cttgtgtgt ttcctgaaa ccgcttttt tcaggaata 
      gtttcagtt gcatgtcaa gcgtgcgtt tgcgctcta 
      gtctttcaa gaaacagtg gcaaataca agtccggca 
      acgctcatc acattgaca catcaaagt gcgacgtta 
      tccttcagg agtcctgac gcccacttc gatgccaac 
      atcctgaaa acaatacaa aaattgcga ctctactca 
      ttgtcaaac cctgcaaca aggtggcgg caaccgacg 
      tgaaagtag cccctgata tgtcaacag ctgtaacaa 
      atggacacc aggcccctt cggaggatt ttgccaaac 
      atgtattcg gagcgcatg aacgtggac acgaattgt 
      tgatgggct tcaccttca ctctcgagt caattttat 
      cgcaacaac gattggttt ctgttattt tgatgatga 
      ttttgttgg cagctgagc tggtggagg aggacacga 
      aaatgtgcg gcaaaatcc taacagtcc tgtcctgga 
      tgatcgtcg ttttgacga tgcccttct ccctcttcg 
      tctgcttta aggtcgttc aagagtacg aaagggccg 
      tcatttttc gcttaggtc gtctcttgt ccggaggcg 
      ccaaagggc caggaattt ttttcatct tgccgtgta 
      ttgatgcct acgccaggg ttgacctgc ggaccagga 
      cctacgaca tccctccac aagaggttc tgaccaaag 
      acagcgtga ccgtctccg tggacgccg tggtctact 
      accgcgtat ccaacgcca ccgtctcca tagccaacg 
      tggagaacg ctcaccact cgactcgcc tcctcgccc 
      agaccaccc tccgcaaca tcatgggcc aacgtcccc 
      tccacgaga tcctcagcg agcgcgaga gcatctccc 
      agcacatga aggccctcc tcgacgagg ccaccgact 
      cctggggca tcaacgtgg aacgagttg aaatcaaag 
      acgtgcgac tgcccatcc agctgcaga gggccatgg 
      cagctgaag ctgaagctg ctcgtgagg cccgtgcta 
      aagtcatcg ccgccgagg gcgagcaga aggcctcca 
      gggccttgc gagaggcct cggaggtga ttggggatt 
      ccccggctg cgctccagc tcagatact tgcagcatg 
      gctcacttg ggcgctttt ctcgcaaac ggggggcta 
      ggggttaag ggtgtctca acttttcgc ttgcgattt 
      gctttaaca agcgtaatg tttgccata acgagtgag 
      cgcggcaaa tttattaca tcactagtt acgtaataa 
      gtgacattt gctgacggg atttgttga aacaccctc 
      ttagtggct tatgttagg cgggagaaa aatgtactt 
      aacttagtt gggtattta gtttagtgg atagtttct 
      agggtggga agtataagg aagattata ttaatctag 
      ttgcttgta ctaattcgg ttcgattcc cactttgac 
      agataatcc gactgtttt atgtttgtc acgggtaac 
      taggatttc tttgacggc atcacgttg tttccctta 
      agtagattt tcctgtcca ctttacacg acattaaaa 
      acttagtcc atcataact aagcttaag cctcatttt 
      catgcaaga tcactcttt cgactcgga aaactttca 
      gtcgtttgt aataagcgt tgggggcaa atggaccgg 
      cttgaatgg tattgatga tggctggga tggaagaca 
      actatgaat tgcgatttg cccttttaa gggatgacg 
      aatggtaaa agggtgcgc gaaaattcg gggattagt 
      tctcgcatg ctgacaacg aaataaata gggaatttt 
      cagtgttat aattattat ttatccgag tacttcaag 
      aattaatct ttgttggca tcattgaac agatcaaac 
      ctcaagtag gttaaaaat aaattacga tttgccaga 
      aatagaagc agaagtcgt tgactgacc attttgaaa 
      aaaactcta caaatttcg gttgatttt tttttacac 
      aaattacgt tatcaattt ttcaatttc ataagcttt 
      aagttttca ttacgttta cgtaagaaa ctcaactct 
      gcttctaat agtgttcgc aaccaaaca acaaagtag 
      ttacctcaa gtaaacaca atctagaat ctctcttac 
      gtagctatg gtttctcca cttgtaatc agtggactt 
      ataatcagt tacattggc cggtattga gtttaatcc 
      gaaacaatt tccagtttt ggtgtcaga tattacaaa 
      ttaatattg aaatacaat catacaatg caactctct 
      atttgcgtt tgattcaac gtaatttcc tcgtcaacg 
      aaacgttta attttgcat tcctgttac agcgaggtt 
      aatcgtctg cgttaaaaa ttcatttta tcaaaagcc 
      atctcatct gtgcagatt tagacagtt actatttgc 
      gaatcgttc atgttcttt ctttggttt tggtttagt 
      ttaatgaaa atgagttag gatgtctga ctatgtgaa 
      ggatttttt taattaaaa atctagtca aaag