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Gene information
General information
TC032654
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003953,TC003952
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid27: 137250..151102
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032654
    iBeetle screen iB_00643
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    iBeetle screen iB_02741
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_02741
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032654 RA
      MHTKDFQFK CDTCNKGFF YRKTLNTHI EFYHKGMRL 
      VCEFCTREF KNNKQMNIH VRKHYDPDH VVTKFPCPE 
      CSKVLSDKY SLKKHVEMI HRGEGGQYV CDICGKRLM 
      TNSSWNNHK RIHAGEKNF VCTECGKAF TTGQAFKVH 
      IRVHTKEKP YKCTECDKA FSQKGSLTL HLRYHTGER 
      PYKCNVCKK QFITNTILK DHKCKGVNN TNAKTYPLD 
      DVQDFYCDV CYKVFPSSI KLTKHRTRH RVKTLKCSL 
      CPKMFLLPS ELKTHISVQ HEKQYNFFC DLCNYSTYK 
      RWSLEVHKI REHTKEYPF KCEVCNKGF VSKVCLTKH 
      KEVQHEGRR FTCAFCSKV FRDEGQFKV HTKRHDPNY 
      VESRYTCTE CLKVFKTKV RWKDHVKMH FEGRNEYIC 
      DICGKSVTT LQSLKDHKT IHTGERNFV CEECGKAFN 
      KKQVLQIHK RVHTKEKPF KCTECEKAF SQRSSLIVH 
      MRYHTGERP YKYQCEVCS KIFPSYRGF RSHQARHNK 
      KHKCPFCEK RYFVPYLLK CHISSTHEN QKKYFCDLC 
      DYATYRKPA LGVHKIREH TKEFPFKCE TCNKGFVYK 
      LFYTRHNQV EHAGLRYTC EVCSKVFRT EKQFKIHVN 
      QHDPNYVEA RYPCTLCLK VFKGKTRWK YHMKMHNEG 
      KREYICDVC GKSMSSMQS LKEHRTIHT GEKKFVCET 
      CAKAFSTKA LLKTHIRVH TKEKPFKCT ECERAFSQR 
      SALVVHLRN KSDPNARFE CEVCHKIFS PWINYRRHT 
      YRHRMKTLM CPYCDRIYR TPNEVESHI TRKHDPSQR 
      VTCEVCNAS LKKLSLKQH LLSHTKDFP HKCDMCDRG 
      FPSKCSLAI HKRAAHEER HLVCEYCAK TFSDNQQFR 
      RHVEKHKPD YVTPAFTCD VCFKVLHVE RSFRKHMKR 
      HRGEVDSYV CDTCGKSMS TLEGFKIHM RTHTGEKPF 
      VCESCGKGF TSKTLLRTH VRVHTKERP YKCTECDKG 
      FTQKGSLNV HMRYHTGER PHKCDFCNK NGIPYSDEV 
      LGNAFECTI CSKRFLTKR KLGKHILRH REKKHKCPL 
      CDRKFISPS EIKTHILYR HDKRKQYFC DHCTFGAPD 
      NFTVKQHTI IHHTRDFPF KCDECTKGF ISRQNLAQH 
      KKVDHEGVE FVCDLCSKR FKSQANLRR HVAKHDPDY 
      TAPSFPCEV CGKVLGKKA SLEYHLKTH KGEASNYVC 
      HLCGKSVTS SMSLKYHMD RHAGKRSFV CEVCGKAFT 
      TKVLLTTHS RVHTKEKPF KCTECDKAF SQKGTLNIH 
      KRVCSSKDL VRRSCPTCY KMVAVGYFT NHLKRHNEK 
      THKCPKCDM MFYSNFEVK SHLSSRHDS TKKTFCNVC 
      NKPFKNNNS LKVHMVSHT QNFPYKCEV CEKGFTHRY 
      LMIDHKVSV HEGVRKVCP FCSKIFINR RLFEMHVAK 
      HNSNYVAEA FTCQVCFKV MRTVSGYKI HMKRHKGEL 
      RSFMCDICG KSLATREAF LNHQLMHSG EKNHICEFC 
      GKAFITKAL LKTHLRVHT KEKPFKCSE CDRAFTQKS 
      SLNVHMRYH TGERPYKCA VCSKQFVTK TILKGHACK 
      GSGFPVSTF FENCDPLRD EAAGVREQL HPKSRYQYM 
      KEYETFSTW RNSNYVDGV TEDILLRYV SDLMEKFAP 
      NSVYTKISM LKTLLKKQE NIDISGYGK VFDLMKRKS 
      KCYKANKAQ KLTAEEVTR YLLEAPDES FLLEKVITV 
      FGIFGACRR EELVTLRAN NIEDTGSLI LVTIENSNN 
      RSCRKFVIT EDETPFKGC ALYRKYAAL RPPGLESQR 
      FFVDYRNGR CTKQLVGIN KISSIPKKI AKFLKLKNP 
      ETYTGHSLR RSGTAVLAE NGVHLLTLK QFRKGRSEE 
      FFEDSTSAL TGGTDVVKQ DDAFETVVK SELDSDSD
    CDS sequence
    • >TC032654 RA
      atgcacacc aaggacttc cagttcaag tgcgacact 
      tgtaataaa ggttttttc tatcgtaaa actttaaac 
      acccacatt gagttctac cataaggga atgcgactc 
      gtgtgcgag ttttgcact agagagttt aagaataac 
      aagcagatg aacatccat gtgagaaag cattatgat 
      cccgaccat gtcgtaact aagtttccc tgtcccgag 
      tgttcgaaa gttttgagt gataaatac agtttgaag 
      aaacacgtg gaaatgata catcggggt gaaggaggg 
      caatatgtg tgtgatatt tgcggaaaa agattgatg 
      actaatagt agttggaac aatcataag aggatacat 
      gctggggag aagaatttt gtgtgtaca gagtgtggt 
      aaagctttt accactgga caggcgttc aaggtacat 
      attagggta catacgaag gaaaaaccg tacaagtgt 
      acggaatgc gataaggcg ttttcacag aagggtagt 
      ttaaccctt catttgcgg taccatact ggtgaaaga 
      ccgtacaag tgtaatgtg tgcaagaaa caatttatt 
      accaatact attctgaaa gaccacaaa tgcaaaggt 
      gttaataat acgaatgcg aaaacatac ccgcttgac 
      gacgtccag gatttttat tgcgacgtt tgctacaaa 
      gtgttccct tcttccatt aaactgaca aaacataga 
      acacgacat cgggtaaaa accctcaaa tgttccttg 
      tgccccaaa atgttcctt ttaccttcc gagttaaaa 
      acgcatatt tcggtccaa cacgagaag caatacaat 
      tttttctgc gacctttgc aactactca acttacaaa 
      aggtggtca cttgaagtt cacaaaata cgcgagcac 
      acgaaagag taccctttc aaatgcgaa gtttgcaac 
      aaaggtttc gtgagtaaa gtgtgtctc actaaacat 
      aaagaagtg caacacgaa ggtcgacgc ttcacttgt 
      gccttttgc tcgaaagtg tttcgagac gaaggccag 
      tttaaagtg cacactaaa cgacacgat cctaactac 
      gttgaaagc aggtacact tgtacagaa tgcttgaaa 
      gttttcaag actaaagtg cggtggaaa gaccacgtg 
      aaaatgcat tttgagggt cgtaatgag tacatttgc 
      gatatttgt ggcaaaagt gttactact ttacaaagc 
      ttgaaagac cacaagacg atacacaca ggtgaaagg 
      aactttgtt tgtgaagaa tgcgggaaa gcttttaac 
      aagaagcaa gtgcttcag attcacaaa cgggttcat 
      acgaaggaa aaaccgttc aaatgtaca gaatgtgag 
      aaggcgttt tctcaaagg agtagtctt atcgtccat 
      atgcggtac cacacgggg gaaaggccg tacaaatac 
      cagtgcgaa gtctgctcc aaaattttt ccttcgtat 
      cgtggattt cgctcacac caagcgcgc cacaacaag 
      aaacacaaa tgccctttt tgcgaaaag cgttacttt 
      gtcccctac ttactcaaa tgccacatt tcttcaacc 
      cacgaaaac cagaaaaaa tatttttgc gacctttgc 
      gattatgcg acttatcgc aaaccagcc ttgggagtc 
      cacaagatt agagaacac acgaaagag tttcccttc 
      aaatgcgag acttgcaac aagggcttt gtgtacaaa 
      ttgttttac acaagacat aaccaagtc gaacacgca 
      ggcttgaga tacacttgt gaagtttgc tcaaaagta 
      ttcagaact gaaaagcaa ttcaaaatt catgtaaac 
      caacacgat ccaaattat gttgaagct aggtaccct 
      tgtactctg tgcttgaag gttttcaaa ggcaaaacg 
      cggtggaaa taccacatg aaaatgcac aacgaaggg 
      aaaagggag tacatttgc gatgtttgt ggcaagagt 
      atgtcaagt atgcaaagt ttgaaggaa catcggact 
      atacatacg ggcgaaaaa aaatttgtg tgcgagact 
      tgtgccaaa gctttcagt accaaagcg ttactgaag 
      actcacata agggttcac accaaggag aagcccttc 
      aagtgcacg gaatgcgaa agggcgttc tctcaaagg 
      agcgctctt gtggtacat ttaaggaac aagtcggat 
      cctaatgcc cgttttgag tgcgaagtt tgccacaaa 
      attttttct ccttggatc aactacagg agacatacc 
      tacagacac agaatgaaa acactgatg tgtccttat 
      tgtgaccgc atataccgg actccaaac gaagtcgaa 
      agtcacata actcgcaaa catgaccct tctcagaga 
      gtcacttgt gaggtgtgc aacgcttct ttgaaaaaa 
      ctctcactt aagcaacac ctcttaagt cacaccaag 
      gattttccg cacaaatgc gatatgtgt gatagaggt 
      ttcccctct aaatgttcc ctagcgatc cacaagcga 
      gcggctcat gaagaacgt catctggtg tgtgaatat 
      tgcgccaaa accttctca gataaccaa caatttcgg 
      cgacatgtc gagaagcac aaacctgat tacgtcact 
      cctgccttc acatgtgat gtgtgcttc aaggtcttg 
      cacgttgag agatctttc aggaagcat atgaaaagg 
      cataggggt gaagttgat agttatgtg tgcgataca 
      tgcggaaaa agtatgtcg actttggaa ggttttaaa 
      attcatatg agaacacac accggagag aaaccattt 
      gtttgtgag tcgtgtggt aagggtttt acttcgaaa 
      accttgctt agaacacat gttagagtt cacaccaag 
      gaacgcccg tataaatgt acagaatgc gataaaggt 
      tttactcaa aagggcagt ttgaacgtc catatgcga 
      taccacacc ggcgaaagg ccccacaag tgcgatttc 
      tgcaacaag aatggcatt ccatactcg gatgaagtt 
      ctcggaaac gcttttgag tgcaccatt tgttcaaaa 
      agattcctc accaaacga aaactaggc aaacacata 
      ttgcgccat cgagaaaaa aaacacaag tgtcctctt 
      tgcgacaga aaatttata tctccttca gaaatcaag 
      actcacatc ttatacaga cacgacaaa cgtaaacag 
      tatttctgc gatcattgt actttcgga gctcctgat 
      aacttcaca gttaaacaa cacacaatt atccatcac 
      actagagat ttccccttt aagtgtgac gagtgcacc 
      aaaggcttc atcagtaga caaaacctg gctcagcac 
      aagaaagtc gaccacgaa ggtgtggaa tttgtgtgt 
      gacttgtgc tcgaaaaga ttcaagagt caggcaaat 
      ctacgaaga catgtcgct aaacacgat cctgattat 
      accgctcct tcatttcca tgtgaagtg tgtggaaaa 
      gttttgggc aagaaagct tccttagaa tatcatttg 
      aaaacgcac aaaggcgag gctagtaat tatgtttgt 
      cacttgtgc gggaaaagt gtcacctct tcaatgagt 
      cttaagtac catatggat agacatgca gggaagaga 
      tcgtttgtt tgtgaagtg tgtggtaag gcatttacc 
      acgaaagta cttcttacc acacactca cgcgtacac 
      acgaaggaa aaaccgttc aagtgtact gaatgtgac 
      aaggcgttt tcccagaag ggtactctc aacatccac 
      aagagggtg tgctcatct aaagattta gtccgccgt 
      tcgtgtcct acctgttac aagatggtt gctgttggg 
      tattttaca aaccatctt aaaagacat aacgaaaag 
      actcacaaa tgtcccaaa tgtgacatg atgttttat 
      tcgaacttt gaggtgaag tcgcattta tcaagtcga 
      catgactct acgaagaaa accttttgt aacgtttgt 
      aacaaaccg ttcaaaaac aataactcg ctaaaagta 
      cacatggtc tctcatacg caaaatttc ccttacaaa 
      tgcgaagtg tgcgaaaaa ggcttcact catagatac 
      ctaatgatc gaccacaag gtgtcggtt cacgaagga 
      gttcgaaaa gtgtgtcct ttttgctca aaaatattt 
      ataaatcgg agattattt gaaatgcac gtggcgaag 
      cacaattcc aactacgtg gctgaagct ttcacgtgt 
      caagtgtgc ttcaaggtg atgcgtact gtttctggg 
      tacaaaata cacatgaaa aggcataaa ggtgagctg 
      cgaagcttc atgtgtgac atttgtggg aaaagcttg 
      gccacaaga gaagctttt ctaaatcat cagttgatg 
      cactctggt gagaagaac cacatttgc gagttttgt 
      ggtaaggct ttcattacg aaagctttg ctgaaaacc 
      cacttaagg gtgcatacg aaggagaag ccgttcaag 
      tgttcagag tgtgataga gcgtttact cagaagagt 
      tcacttaat gttcacatg cggtaccat accggggaa 
      aggccttac aagtgtgcc gtgtgtagt aaacagttt 
      gttaccaaa actattcta aagggccat gcctgtaaa 
      ggctcaggg tttcctgtg tcaacattt ttcgaaaat 
      tgcgatccc ttacgggat gaagcagct ggagtaaga 
      gagcagtta caccctaaa tcaaggtat cagtatatg 
      aaagaatac gagactttt tcgacgtgg cgtaacagt 
      aattatgtt gatggtgta accgaggat atcttgcta 
      cgttatgta tccgactta atggaaaag tttgcaccg 
      aacagcgtc tacaccaaa atatccatg ttaaaaaca 
      ttgttaaag aaacaagaa aatattgat atttctggt 
      tatgggaaa gttttcgat ttgatgaag cgaaaatcg 
      aaatgttac aaagcaaat aaagcacaa aagcttaca 
      gcggaagaa gtgacgaga tatctactg gaagctcca 
      gacgagagt ttcttgctt gaaaaagtt atcacagta 
      tttggaatt tttggcgct tgtcgacgc gaggagctc 
      gttactttg cgcgcgaat aatattgaa gacactggt 
      agtcttata cttgtgaca atagaaaat tcaaataat 
      cggtcttgc cgaaagttt gtaataact gaggacgag 
      acacctttt aaaggttgt gctttgtac aggaaatac 
      gcagcactt agacctccc gggttggag agtcagcgc 
      ttttttgtg gactaccgg aacggtaga tgcacaaaa 
      cagttagta ggtattaac aaaatttca agtattccg 
      aaaaaaatc gccaagttc ttaaaactg aaaaatcct 
      gaaacgtac acgggtcat tctttgagg agatctggg 
      actgctgtg ttggccgaa aatggggtt cacctcctg 
      actctgaaa cagtttcgt aaaggcagg tcagaggag 
      ttttttgaa gacagtact tcagcactt acaggtggt 
      actgacgtt gtaaaacaa gacgatgct tttgaaacc 
      gtagttaag tctgaattg gatagcgat tcagactaa
    mRNA sequence
    • >TC032654 RA
      cgcccacat aactagaat gcacaccaa ggacttcca 
      gttcaagtg cgacacttg taataaagg ttttttcta 
      tcgtaaaac tttaaacac ccacattga gttctacca 
      taagggaat gcgactcgt gtgcgagtt ttgcactag 
      agagtttaa gaataacaa gcagatgaa catccatgt 
      gagaaagca ttatgatcc cgaccatgt cgtaactaa 
      gtttccctg tcccgagtg ttcgaaagt tttgagtga 
      taaatacag tttgaagaa acacgtgga aatgataca 
      tcggggtga aggagggca atatgtgtg tgatatttg 
      cggaaaaag attgatgac taatagtag ttggaacaa 
      tcataagag gatacatgc tggggagaa gaattttgt 
      gtgtacaga gtgtggtaa agcttttac cactggaca 
      ggcgttcaa ggtacatat tagggtaca tacgaagga 
      aaaaccgta caagtgtac ggaatgcga taaggcgtt 
      ttcacagaa gggtagttt aacccttca tttgcggta 
      ccatactgg tgaaagacc gtacaagtg taatgtgtg 
      caagaaaca atttattac caatactat tctgaaaga 
      ccacaaatg caaaggtgt taataatac gaatgcgaa 
      aacataccc gcttgacga cgtccagga tttttattg 
      cgacgtttg ctacaaagt gttcccttc ttccattaa 
      actgacaaa acatagaac acgacatcg ggtaaaaac 
      cctcaaatg ttccttgtg ccccaaaat gttcctttt 
      accttccga gttaaaaac gcatatttc ggtccaaca 
      cgagaagca atacaattt tttctgcga cctttgcaa 
      ctactcaac ttacaaaag gtggtcact tgaagttca 
      caaaatacg cgagcacac gaaagagta ccctttcaa 
      atgcgaagt ttgcaacaa aggtttcgt gagtaaagt 
      gtgtctcac taaacataa agaagtgca acacgaagg 
      tcgacgctt cacttgtgc cttttgctc gaaagtgtt 
      tcgagacga aggccagtt taaagtgca cactaaacg 
      acacgatcc taactacgt tgaaagcag gtacacttg 
      tacagaatg cttgaaagt tttcaagac taaagtgcg 
      gtggaaaga ccacgtgaa aatgcattt tgagggtcg 
      taatgagta catttgcga tatttgtgg caaaagtgt 
      tactacttt acaaagctt gaaagacca caagacgat 
      acacacagg tgaaaggaa ctttgtttg tgaagaatg 
      cgggaaagc ttttaacaa gaagcaagt gcttcagat 
      tcacaaacg ggttcatac gaaggaaaa accgttcaa 
      atgtacaga atgtgagaa ggcgttttc tcaaaggag 
      tagtcttat cgtccatat gcggtacca cacggggga 
      aaggccgta caaatacca gtgcgaagt ctgctccaa 
      aatttttcc ttcgtatcg tggatttcg ctcacacca 
      agcgcgcca caacaagaa acacaaatg ccctttttg 
      cgaaaagcg ttactttgt cccctactt actcaaatg 
      ccacatttc ttcaaccca cgaaaacca gaaaaaata 
      tttttgcga cctttgcga ttatgcgac ttatcgcaa 
      accagcctt gggagtcca caagattag agaacacac 
      gaaagagtt tcccttcaa atgcgagac ttgcaacaa 
      gggctttgt gtacaaatt gttttacac aagacataa 
      ccaagtcga acacgcagg cttgagata cacttgtga 
      agtttgctc aaaagtatt cagaactga aaagcaatt 
      caaaattca tgtaaacca acacgatcc aaattatgt 
      tgaagctag gtacccttg tactctgtg cttgaaggt 
      tttcaaagg caaaacgcg gtggaaata ccacatgaa 
      aatgcacaa cgaagggaa aagggagta catttgcga 
      tgtttgtgg caagagtat gtcaagtat gcaaagttt 
      gaaggaaca tcggactat acatacggg cgaaaaaaa 
      atttgtgtg cgagacttg tgccaaagc tttcagtac 
      caaagcgtt actgaagac tcacataag ggttcacac 
      caaggagaa gcccttcaa gtgcacgga atgcgaaag 
      ggcgttctc tcaaaggag cgctcttgt ggtacattt 
      aaggaacaa gtcggatcc taatgcccg ttttgagtg 
      cgaagtttg ccacaaaat tttttctcc ttggatcaa 
      ctacaggag acataccta cagacacag aatgaaaac 
      actgatgtg tccttattg tgaccgcat ataccggac 
      tccaaacga agtcgaaag tcacataac tcgcaaaca 
      tgacccttc tcagagagt cacttgtga ggtgtgcaa 
      cgcttcttt gaaaaaact ctcacttaa gcaacacct 
      cttaagtca caccaagga ttttccgca caaatgcga 
      tatgtgtga tagaggttt cccctctaa atgttccct 
      agcgatcca caagcgagc ggctcatga agaacgtca 
      tctggtgtg tgaatattg cgccaaaac cttctcaga 
      taaccaaca atttcggcg acatgtcga gaagcacaa 
      acctgatta cgtcactcc tgccttcac atgtgatgt 
      gtgcttcaa ggtcttgca cgttgagag atctttcag 
      gaagcatat gaaaaggca taggggtga agttgatag 
      ttatgtgtg cgatacatg cggaaaaag tatgtcgac 
      tttggaagg ttttaaaat tcatatgag aacacacac 
      cggagagaa accatttgt ttgtgagtc gtgtggtaa 
      gggttttac ttcgaaaac cttgcttag aacacatgt 
      tagagttca caccaagga acgcccgta taaatgtac 
      agaatgcga taaaggttt tactcaaaa gggcagttt 
      gaacgtcca tatgcgata ccacaccgg cgaaaggcc 
      ccacaagtg cgatttctg caacaagaa tggcattcc 
      atactcgga tgaagttct cggaaacgc ttttgagtg 
      caccatttg ttcaaaaag attcctcac caaacgaaa 
      actaggcaa acacatatt gcgccatcg agaaaaaaa 
      acacaagtg tcctctttg cgacagaaa atttatatc 
      tccttcaga aatcaagac tcacatctt atacagaca 
      cgacaaacg taaacagta tttctgcga tcattgtac 
      tttcggagc tcctgataa cttcacagt taaacaaca 
      cacaattat ccatcacac tagagattt cccctttaa 
      gtgtgacga gtgcaccaa aggcttcat cagtagaca 
      aaacctggc tcagcacaa gaaagtcga ccacgaagg 
      tgtggaatt tgtgtgtga cttgtgctc gaaaagatt 
      caagagtca ggcaaatct acgaagaca tgtcgctaa 
      acacgatcc tgattatac cgctccttc atttccatg 
      tgaagtgtg tggaaaagt tttgggcaa gaaagcttc 
      cttagaata tcatttgaa aacgcacaa aggcgaggc 
      tagtaatta tgtttgtca cttgtgcgg gaaaagtgt 
      cacctcttc aatgagtct taagtacca tatggatag 
      acatgcagg gaagagatc gtttgtttg tgaagtgtg 
      tggtaaggc atttaccac gaaagtact tcttaccac 
      acactcacg cgtacacac gaaggaaaa accgttcaa 
      gtgtactga atgtgacaa ggcgttttc ccagaaggg 
      tactctcaa catccacaa gagggtgtg ctcatctaa 
      agatttagt ccgccgttc gtgtcctac ctgttacaa 
      gatggttgc tgttgggta ttttacaaa ccatcttaa 
      aagacataa cgaaaagac tcacaaatg tcccaaatg 
      tgacatgat gttttattc gaactttga ggtgaagtc 
      gcatttatc aagtcgaca tgactctac gaagaaaac 
      cttttgtaa cgtttgtaa caaaccgtt caaaaacaa 
      taactcgct aaaagtaca catggtctc tcatacgca 
      aaatttccc ttacaaatg cgaagtgtg cgaaaaagg 
      cttcactca tagatacct aatgatcga ccacaaggt 
      gtcggttca cgaaggagt tcgaaaagt gtgtccttt 
      ttgctcaaa aatatttat aaatcggag attatttga 
      aatgcacgt ggcgaagca caattccaa ctacgtggc 
      tgaagcttt cacgtgtca agtgtgctt caaggtgat 
      gcgtactgt ttctgggta caaaataca catgaaaag 
      gcataaagg tgagctgcg aagcttcat gtgtgacat 
      ttgtgggaa aagcttggc cacaagaga agcttttct 
      aaatcatca gttgatgca ctctggtga gaagaacca 
      catttgcga gttttgtgg taaggcttt cattacgaa 
      agctttgct gaaaaccca cttaagggt gcatacgaa 
      ggagaagcc gttcaagtg ttcagagtg tgatagagc 
      gtttactca gaagagttc acttaatgt tcacatgcg 
      gtaccatac cggggaaag gccttacaa gtgtgccgt 
      gtgtagtaa acagtttgt taccaaaac tattctaaa 
      gggccatgc ctgtaaagg ctcagggtt tcctgtgtc 
      aacattttt cgaaaattg cgatccctt acgggatga 
      agcagctgg agtaagaga gcagttaca ccctaaatc 
      aaggtatca gtatatgaa agaatacga gactttttc 
      gacgtggcg taacagtaa ttatgttga tggtgtaac 
      cgaggatat cttgctacg ttatgtatc cgacttaat 
      ggaaaagtt tgcaccgaa cagcgtcta caccaaaat 
      atccatgtt aaaaacatt gttaaagaa acaagaaaa 
      tattgatat ttctggtta tgggaaagt tttcgattt 
      gatgaagcg aaaatcgaa atgttacaa agcaaataa 
      agcacaaaa gcttacagc ggaagaagt gacgagata 
      tctactgga agctccaga cgagagttt cttgcttga 
      aaaagttat cacagtatt tggaatttt tggcgcttg 
      tcgacgcga ggagctcgt tactttgcg cgcgaataa 
      tattgaaga cactggtag tcttatact tgtgacaat 
      agaaaattc aaataatcg gtcttgccg aaagtttgt 
      aataactga ggacgagac accttttaa aggttgtgc 
      tttgtacag gaaatacgc agcacttag acctcccgg 
      gttggagag tcagcgctt ttttgtgga ctaccggaa 
      cggtagatg cacaaaaca gttagtagg tattaacaa 
      aatttcaag tattccgaa aaaaatcgc caagttctt 
      aaaactgaa aaatcctga aacgtacac gggtcattc 
      tttgaggag atctgggac tgctgtgtt ggccgaaaa 
      tggggttca cctcctgac tctgaaaca gtttcgtaa 
      aggcaggtc agaggagtt ttttgaaga cagtacttc 
      agcacttac aggtggtac tgacgttgt aaaacaaga 
      cgatgcttt tgaaaccgt agttaagtc tgaattgga 
      tagcgattc agactaatt tgttgtaat ttgaataaa 
      tgtattttt ttaaa