General information | |||
TC032654
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 27974806..27988658 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC032654 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00643 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02741 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (9 dpi)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02741 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: % (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC032654 RA
MHTKDFQFK CDTCNKGFF YRKTLNTHI EFYHKGMRL VCEFCTREF KNNKQMNIH VRKHYDPDH VVTKFPCPE CSKVLSDKY SLKKHVEMI HRGEGGQYV CDICGKRLM TNSSWNNHK RIHAGEKNF VCTECGKAF TTGQAFKVH IRVHTKEKP YKCTECDKA FSQKGSLTL HLRYHTGER PYKCNVCKK QFITNTILK DHKCKGVNN TNAKTYPLD DVQDFYCDV CYKVFPSSI KLTKHRTRH RVKTLKCSL CPKMFLLPS ELKTHISVQ HEKQYNFFC DLCNYSTYK RWSLEVHKI REHTKEYPF KCEVCNKGF VSKVCLTKH KEVQHEGRR FTCAFCSKV FRDEGQFKV HTKRHDPNY VESRYTCTE CLKVFKTKV RWKDHVKMH FEGRNEYIC DICGKSVTT LQSLKDHKT IHTGERNFV CEECGKAFN KKQVLQIHK RVHTKEKPF KCTECEKAF SQRSSLIVH MRYHTGERP YKYQCEVCS KIFPSYRGF RSHQARHNK KHKCPFCEK RYFVPYLLK CHISSTHEN QKKYFCDLC DYATYRKPA LGVHKIREH TKEFPFKCE TCNKGFVYK LFYTRHNQV EHAGLRYTC EVCSKVFRT EKQFKIHVN QHDPNYVEA RYPCTLCLK VFKGKTRWK YHMKMHNEG KREYICDVC GKSMSSMQS LKEHRTIHT GEKKFVCET CAKAFSTKA LLKTHIRVH TKEKPFKCT ECERAFSQR SALVVHLRN KSDPNARFE CEVCHKIFS PWINYRRHT YRHRMKTLM CPYCDRIYR TPNEVESHI TRKHDPSQR VTCEVCNAS LKKLSLKQH LLSHTKDFP HKCDMCDRG FPSKCSLAI HKRAAHEER HLVCEYCAK TFSDNQQFR RHVEKHKPD YVTPAFTCD VCFKVLHVE RSFRKHMKR HRGEVDSYV CDTCGKSMS TLEGFKIHM RTHTGEKPF VCESCGKGF TSKTLLRTH VRVHTKERP YKCTECDKG FTQKGSLNV HMRYHTGER PHKCDFCNK NGIPYSDEV LGNAFECTI CSKRFLTKR KLGKHILRH REKKHKCPL CDRKFISPS EIKTHILYR HDKRKQYFC DHCTFGAPD NFTVKQHTI IHHTRDFPF KCDECTKGF ISRQNLAQH KKVDHEGVE FVCDLCSKR FKSQANLRR HVAKHDPDY TAPSFPCEV CGKVLGKKA SLEYHLKTH KGEASNYVC HLCGKSVTS SMSLKYHMD RHAGKRSFV CEVCGKAFT TKVLLTTHS RVHTKEKPF KCTECDKAF SQKGTLNIH KRVCSSKDL VRRSCPTCY KMVAVGYFT NHLKRHNEK THKCPKCDM MFYSNFEVK SHLSSRHDS TKKTFCNVC NKPFKNNNS LKVHMVSHT QNFPYKCEV CEKGFTHRY LMIDHKVSV HEGVRKVCP FCSKIFINR RLFEMHVAK HNSNYVAEA FTCQVCFKV MRTVSGYKI HMKRHKGEL RSFMCDICG KSLATREAF LNHQLMHSG EKNHICEFC GKAFITKAL LKTHLRVHT KEKPFKCSE CDRAFTQKS SLNVHMRYH TGERPYKCA VCSKQFVTK TILKGHACK GSGFPVSTF FENCDPLRD EAAGVREQL HPKSRYQYM KEYETFSTW RNSNYVDGV TEDILLRYV SDLMEKFAP NSVYTKISM LKTLLKKQE NIDISGYGK VFDLMKRKS KCYKANKAQ KLTAEEVTR YLLEAPDES FLLEKVITV FGIFGACRR EELVTLRAN NIEDTGSLI LVTIENSNN RSCRKFVIT EDETPFKGC ALYRKYAAL RPPGLESQR FFVDYRNGR CTKQLVGIN KISSIPKKI AKFLKLKNP ETYTGHSLR RSGTAVLAE NGVHLLTLK QFRKGRSEE FFEDSTSAL TGGTDVVKQ DDAFETVVK SELDSDSD
- >TC032654 RA
atgcacacc aaggacttc cagttcaag tgcgacact tgtaataaa ggttttttc tatcgtaaa actttaaac acccacatt gagttctac cataaggga atgcgactc gtgtgcgag ttttgcact agagagttt aagaataac aagcagatg aacatccat gtgagaaag cattatgat cccgaccat gtcgtaact aagtttccc tgtcccgag tgttcgaaa gttttgagt gataaatac agtttgaag aaacacgtg gaaatgata catcggggt gaaggaggg caatatgtg tgtgatatt tgcggaaaa agattgatg actaatagt agttggaac aatcataag aggatacat gctggggag aagaatttt gtgtgtaca gagtgtggt aaagctttt accactgga caggcgttc aaggtacat attagggta catacgaag gaaaaaccg tacaagtgt acggaatgc gataaggcg ttttcacag aagggtagt ttaaccctt catttgcgg taccatact ggtgaaaga ccgtacaag tgtaatgtg tgcaagaaa caatttatt accaatact attctgaaa gaccacaaa tgcaaaggt gttaataat acgaatgcg aaaacatac ccgcttgac gacgtccag gatttttat tgcgacgtt tgctacaaa gtgttccct tcttccatt aaactgaca aaacataga acacgacat cgggtaaaa accctcaaa tgttccttg tgccccaaa atgttcctt ttaccttcc gagttaaaa acgcatatt tcggtccaa cacgagaag caatacaat tttttctgc gacctttgc aactactca acttacaaa aggtggtca cttgaagtt cacaaaata cgcgagcac acgaaagag taccctttc aaatgcgaa gtttgcaac aaaggtttc gtgagtaaa gtgtgtctc actaaacat aaagaagtg caacacgaa ggtcgacgc ttcacttgt gccttttgc tcgaaagtg tttcgagac gaaggccag tttaaagtg cacactaaa cgacacgat cctaactac gttgaaagc aggtacact tgtacagaa tgcttgaaa gttttcaag actaaagtg cggtggaaa gaccacgtg aaaatgcat tttgagggt cgtaatgag tacatttgc gatatttgt ggcaaaagt gttactact ttacaaagc ttgaaagac cacaagacg atacacaca ggtgaaagg aactttgtt tgtgaagaa tgcgggaaa gcttttaac aagaagcaa gtgcttcag attcacaaa cgggttcat acgaaggaa aaaccgttc aaatgtaca gaatgtgag aaggcgttt tctcaaagg agtagtctt atcgtccat atgcggtac cacacgggg gaaaggccg tacaaatac cagtgcgaa gtctgctcc aaaattttt ccttcgtat cgtggattt cgctcacac caagcgcgc cacaacaag aaacacaaa tgccctttt tgcgaaaag cgttacttt gtcccctac ttactcaaa tgccacatt tcttcaacc cacgaaaac cagaaaaaa tatttttgc gacctttgc gattatgcg acttatcgc aaaccagcc ttgggagtc cacaagatt agagaacac acgaaagag tttcccttc aaatgcgag acttgcaac aagggcttt gtgtacaaa ttgttttac acaagacat aaccaagtc gaacacgca ggcttgaga tacacttgt gaagtttgc tcaaaagta ttcagaact gaaaagcaa ttcaaaatt catgtaaac caacacgat ccaaattat gttgaagct aggtaccct tgtactctg tgcttgaag gttttcaaa ggcaaaacg cggtggaaa taccacatg aaaatgcac aacgaaggg aaaagggag tacatttgc gatgtttgt ggcaagagt atgtcaagt atgcaaagt ttgaaggaa catcggact atacatacg ggcgaaaaa aaatttgtg tgcgagact tgtgccaaa gctttcagt accaaagcg ttactgaag actcacata agggttcac accaaggag aagcccttc aagtgcacg gaatgcgaa agggcgttc tctcaaagg agcgctctt gtggtacat ttaaggaac aagtcggat cctaatgcc cgttttgag tgcgaagtt tgccacaaa attttttct ccttggatc aactacagg agacatacc tacagacac agaatgaaa acactgatg tgtccttat tgtgaccgc atataccgg actccaaac gaagtcgaa agtcacata actcgcaaa catgaccct tctcagaga gtcacttgt gaggtgtgc aacgcttct ttgaaaaaa ctctcactt aagcaacac ctcttaagt cacaccaag gattttccg cacaaatgc gatatgtgt gatagaggt ttcccctct aaatgttcc ctagcgatc cacaagcga gcggctcat gaagaacgt catctggtg tgtgaatat tgcgccaaa accttctca gataaccaa caatttcgg cgacatgtc gagaagcac aaacctgat tacgtcact cctgccttc acatgtgat gtgtgcttc aaggtcttg cacgttgag agatctttc aggaagcat atgaaaagg cataggggt gaagttgat agttatgtg tgcgataca tgcggaaaa agtatgtcg actttggaa ggttttaaa attcatatg agaacacac accggagag aaaccattt gtttgtgag tcgtgtggt aagggtttt acttcgaaa accttgctt agaacacat gttagagtt cacaccaag gaacgcccg tataaatgt acagaatgc gataaaggt tttactcaa aagggcagt ttgaacgtc catatgcga taccacacc ggcgaaagg ccccacaag tgcgatttc tgcaacaag aatggcatt ccatactcg gatgaagtt ctcggaaac gcttttgag tgcaccatt tgttcaaaa agattcctc accaaacga aaactaggc aaacacata ttgcgccat cgagaaaaa aaacacaag tgtcctctt tgcgacaga aaatttata tctccttca gaaatcaag actcacatc ttatacaga cacgacaaa cgtaaacag tatttctgc gatcattgt actttcgga gctcctgat aacttcaca gttaaacaa cacacaatt atccatcac actagagat ttccccttt aagtgtgac gagtgcacc aaaggcttc atcagtaga caaaacctg gctcagcac aagaaagtc gaccacgaa ggtgtggaa tttgtgtgt gacttgtgc tcgaaaaga ttcaagagt caggcaaat ctacgaaga catgtcgct aaacacgat cctgattat accgctcct tcatttcca tgtgaagtg tgtggaaaa gttttgggc aagaaagct tccttagaa tatcatttg aaaacgcac aaaggcgag gctagtaat tatgtttgt cacttgtgc gggaaaagt gtcacctct tcaatgagt cttaagtac catatggat agacatgca gggaagaga tcgtttgtt tgtgaagtg tgtggtaag gcatttacc acgaaagta cttcttacc acacactca cgcgtacac acgaaggaa aaaccgttc aagtgtact gaatgtgac aaggcgttt tcccagaag ggtactctc aacatccac aagagggtg tgctcatct aaagattta gtccgccgt tcgtgtcct acctgttac aagatggtt gctgttggg tattttaca aaccatctt aaaagacat aacgaaaag actcacaaa tgtcccaaa tgtgacatg atgttttat tcgaacttt gaggtgaag tcgcattta tcaagtcga catgactct acgaagaaa accttttgt aacgtttgt aacaaaccg ttcaaaaac aataactcg ctaaaagta cacatggtc tctcatacg caaaatttc ccttacaaa tgcgaagtg tgcgaaaaa ggcttcact catagatac ctaatgatc gaccacaag gtgtcggtt cacgaagga gttcgaaaa gtgtgtcct ttttgctca aaaatattt ataaatcgg agattattt gaaatgcac gtggcgaag cacaattcc aactacgtg gctgaagct ttcacgtgt caagtgtgc ttcaaggtg atgcgtact gtttctggg tacaaaata cacatgaaa aggcataaa ggtgagctg cgaagcttc atgtgtgac atttgtggg aaaagcttg gccacaaga gaagctttt ctaaatcat cagttgatg cactctggt gagaagaac cacatttgc gagttttgt ggtaaggct ttcattacg aaagctttg ctgaaaacc cacttaagg gtgcatacg aaggagaag ccgttcaag tgttcagag tgtgataga gcgtttact cagaagagt tcacttaat gttcacatg cggtaccat accggggaa aggccttac aagtgtgcc gtgtgtagt aaacagttt gttaccaaa actattcta aagggccat gcctgtaaa ggctcaggg tttcctgtg tcaacattt ttcgaaaat tgcgatccc ttacgggat gaagcagct ggagtaaga gagcagtta caccctaaa tcaaggtat cagtatatg aaagaatac gagactttt tcgacgtgg cgtaacagt aattatgtt gatggtgta accgaggat atcttgcta cgttatgta tccgactta atggaaaag tttgcaccg aacagcgtc tacaccaaa atatccatg ttaaaaaca ttgttaaag aaacaagaa aatattgat atttctggt tatgggaaa gttttcgat ttgatgaag cgaaaatcg aaatgttac aaagcaaat aaagcacaa aagcttaca gcggaagaa gtgacgaga tatctactg gaagctcca gacgagagt ttcttgctt gaaaaagtt atcacagta tttggaatt tttggcgct tgtcgacgc gaggagctc gttactttg cgcgcgaat aatattgaa gacactggt agtcttata cttgtgaca atagaaaat tcaaataat cggtcttgc cgaaagttt gtaataact gaggacgag acacctttt aaaggttgt gctttgtac aggaaatac gcagcactt agacctccc gggttggag agtcagcgc ttttttgtg gactaccgg aacggtaga tgcacaaaa cagttagta ggtattaac aaaatttca agtattccg aaaaaaatc gccaagttc ttaaaactg aaaaatcct gaaacgtac acgggtcat tctttgagg agatctggg actgctgtg ttggccgaa aatggggtt cacctcctg actctgaaa cagtttcgt aaaggcagg tcagaggag ttttttgaa gacagtact tcagcactt acaggtggt actgacgtt gtaaaacaa gacgatgct tttgaaacc gtagttaag tctgaattg gatagcgat tcagactaa
- >TC032654 RA
cgcccacat aactagaat gcacaccaa ggacttcca gttcaagtg cgacacttg taataaagg ttttttcta tcgtaaaac tttaaacac ccacattga gttctacca taagggaat gcgactcgt gtgcgagtt ttgcactag agagtttaa gaataacaa gcagatgaa catccatgt gagaaagca ttatgatcc cgaccatgt cgtaactaa gtttccctg tcccgagtg ttcgaaagt tttgagtga taaatacag tttgaagaa acacgtgga aatgataca tcggggtga aggagggca atatgtgtg tgatatttg cggaaaaag attgatgac taatagtag ttggaacaa tcataagag gatacatgc tggggagaa gaattttgt gtgtacaga gtgtggtaa agcttttac cactggaca ggcgttcaa ggtacatat tagggtaca tacgaagga aaaaccgta caagtgtac ggaatgcga taaggcgtt ttcacagaa gggtagttt aacccttca tttgcggta ccatactgg tgaaagacc gtacaagtg taatgtgtg caagaaaca atttattac caatactat tctgaaaga ccacaaatg caaaggtgt taataatac gaatgcgaa aacataccc gcttgacga cgtccagga tttttattg cgacgtttg ctacaaagt gttcccttc ttccattaa actgacaaa acatagaac acgacatcg ggtaaaaac cctcaaatg ttccttgtg ccccaaaat gttcctttt accttccga gttaaaaac gcatatttc ggtccaaca cgagaagca atacaattt tttctgcga cctttgcaa ctactcaac ttacaaaag gtggtcact tgaagttca caaaatacg cgagcacac gaaagagta ccctttcaa atgcgaagt ttgcaacaa aggtttcgt gagtaaagt gtgtctcac taaacataa agaagtgca acacgaagg tcgacgctt cacttgtgc cttttgctc gaaagtgtt tcgagacga aggccagtt taaagtgca cactaaacg acacgatcc taactacgt tgaaagcag gtacacttg tacagaatg cttgaaagt tttcaagac taaagtgcg gtggaaaga ccacgtgaa aatgcattt tgagggtcg taatgagta catttgcga tatttgtgg caaaagtgt tactacttt acaaagctt gaaagacca caagacgat acacacagg tgaaaggaa ctttgtttg tgaagaatg cgggaaagc ttttaacaa gaagcaagt gcttcagat tcacaaacg ggttcatac gaaggaaaa accgttcaa atgtacaga atgtgagaa ggcgttttc tcaaaggag tagtcttat cgtccatat gcggtacca cacggggga aaggccgta caaatacca gtgcgaagt ctgctccaa aatttttcc ttcgtatcg tggatttcg ctcacacca agcgcgcca caacaagaa acacaaatg ccctttttg cgaaaagcg ttactttgt cccctactt actcaaatg ccacatttc ttcaaccca cgaaaacca gaaaaaata tttttgcga cctttgcga ttatgcgac ttatcgcaa accagcctt gggagtcca caagattag agaacacac gaaagagtt tcccttcaa atgcgagac ttgcaacaa gggctttgt gtacaaatt gttttacac aagacataa ccaagtcga acacgcagg cttgagata cacttgtga agtttgctc aaaagtatt cagaactga aaagcaatt caaaattca tgtaaacca acacgatcc aaattatgt tgaagctag gtacccttg tactctgtg cttgaaggt tttcaaagg caaaacgcg gtggaaata ccacatgaa aatgcacaa cgaagggaa aagggagta catttgcga tgtttgtgg caagagtat gtcaagtat gcaaagttt gaaggaaca tcggactat acatacggg cgaaaaaaa atttgtgtg cgagacttg tgccaaagc tttcagtac caaagcgtt actgaagac tcacataag ggttcacac caaggagaa gcccttcaa gtgcacgga atgcgaaag ggcgttctc tcaaaggag cgctcttgt ggtacattt aaggaacaa gtcggatcc taatgcccg ttttgagtg cgaagtttg ccacaaaat tttttctcc ttggatcaa ctacaggag acataccta cagacacag aatgaaaac actgatgtg tccttattg tgaccgcat ataccggac tccaaacga agtcgaaag tcacataac tcgcaaaca tgacccttc tcagagagt cacttgtga ggtgtgcaa cgcttcttt gaaaaaact ctcacttaa gcaacacct cttaagtca caccaagga ttttccgca caaatgcga tatgtgtga tagaggttt cccctctaa atgttccct agcgatcca caagcgagc ggctcatga agaacgtca tctggtgtg tgaatattg cgccaaaac cttctcaga taaccaaca atttcggcg acatgtcga gaagcacaa acctgatta cgtcactcc tgccttcac atgtgatgt gtgcttcaa ggtcttgca cgttgagag atctttcag gaagcatat gaaaaggca taggggtga agttgatag ttatgtgtg cgatacatg cggaaaaag tatgtcgac tttggaagg ttttaaaat tcatatgag aacacacac cggagagaa accatttgt ttgtgagtc gtgtggtaa gggttttac ttcgaaaac cttgcttag aacacatgt tagagttca caccaagga acgcccgta taaatgtac agaatgcga taaaggttt tactcaaaa gggcagttt gaacgtcca tatgcgata ccacaccgg cgaaaggcc ccacaagtg cgatttctg caacaagaa tggcattcc atactcgga tgaagttct cggaaacgc ttttgagtg caccatttg ttcaaaaag attcctcac caaacgaaa actaggcaa acacatatt gcgccatcg agaaaaaaa acacaagtg tcctctttg cgacagaaa atttatatc tccttcaga aatcaagac tcacatctt atacagaca cgacaaacg taaacagta tttctgcga tcattgtac tttcggagc tcctgataa cttcacagt taaacaaca cacaattat ccatcacac tagagattt cccctttaa gtgtgacga gtgcaccaa aggcttcat cagtagaca aaacctggc tcagcacaa gaaagtcga ccacgaagg tgtggaatt tgtgtgtga cttgtgctc gaaaagatt caagagtca ggcaaatct acgaagaca tgtcgctaa acacgatcc tgattatac cgctccttc atttccatg tgaagtgtg tggaaaagt tttgggcaa gaaagcttc cttagaata tcatttgaa aacgcacaa aggcgaggc tagtaatta tgtttgtca cttgtgcgg gaaaagtgt cacctcttc aatgagtct taagtacca tatggatag acatgcagg gaagagatc gtttgtttg tgaagtgtg tggtaaggc atttaccac gaaagtact tcttaccac acactcacg cgtacacac gaaggaaaa accgttcaa gtgtactga atgtgacaa ggcgttttc ccagaaggg tactctcaa catccacaa gagggtgtg ctcatctaa agatttagt ccgccgttc gtgtcctac ctgttacaa gatggttgc tgttgggta ttttacaaa ccatcttaa aagacataa cgaaaagac tcacaaatg tcccaaatg tgacatgat gttttattc gaactttga ggtgaagtc gcatttatc aagtcgaca tgactctac gaagaaaac cttttgtaa cgtttgtaa caaaccgtt caaaaacaa taactcgct aaaagtaca catggtctc tcatacgca aaatttccc ttacaaatg cgaagtgtg cgaaaaagg cttcactca tagatacct aatgatcga ccacaaggt gtcggttca cgaaggagt tcgaaaagt gtgtccttt ttgctcaaa aatatttat aaatcggag attatttga aatgcacgt ggcgaagca caattccaa ctacgtggc tgaagcttt cacgtgtca agtgtgctt caaggtgat gcgtactgt ttctgggta caaaataca catgaaaag gcataaagg tgagctgcg aagcttcat gtgtgacat ttgtgggaa aagcttggc cacaagaga agcttttct aaatcatca gttgatgca ctctggtga gaagaacca catttgcga gttttgtgg taaggcttt cattacgaa agctttgct gaaaaccca cttaagggt gcatacgaa ggagaagcc gttcaagtg ttcagagtg tgatagagc gtttactca gaagagttc acttaatgt tcacatgcg gtaccatac cggggaaag gccttacaa gtgtgccgt gtgtagtaa acagtttgt taccaaaac tattctaaa gggccatgc ctgtaaagg ctcagggtt tcctgtgtc aacattttt cgaaaattg cgatccctt acgggatga agcagctgg agtaagaga gcagttaca ccctaaatc aaggtatca gtatatgaa agaatacga gactttttc gacgtggcg taacagtaa ttatgttga tggtgtaac cgaggatat cttgctacg ttatgtatc cgacttaat ggaaaagtt tgcaccgaa cagcgtcta caccaaaat atccatgtt aaaaacatt gttaaagaa acaagaaaa tattgatat ttctggtta tgggaaagt tttcgattt gatgaagcg aaaatcgaa atgttacaa agcaaataa agcacaaaa gcttacagc ggaagaagt gacgagata tctactgga agctccaga cgagagttt cttgcttga aaaagttat cacagtatt tggaatttt tggcgcttg tcgacgcga ggagctcgt tactttgcg cgcgaataa tattgaaga cactggtag tcttatact tgtgacaat agaaaattc aaataatcg gtcttgccg aaagtttgt aataactga ggacgagac accttttaa aggttgtgc tttgtacag gaaatacgc agcacttag acctcccgg gttggagag tcagcgctt ttttgtgga ctaccggaa cggtagatg cacaaaaca gttagtagg tattaacaa aatttcaag tattccgaa aaaaatcgc caagttctt aaaactgaa aaatcctga aacgtacac gggtcattc tttgaggag atctgggac tgctgtgtt ggccgaaaa tggggttca cctcctgac tctgaaaca gtttcgtaa aggcaggtc agaggagtt ttttgaaga cagtacttc agcacttac aggtggtac tgacgttgt aaaacaaga cgatgcttt tgaaaccgt agttaagtc tgaattgga tagcgattc agactaatt tgttgtaat ttgaataaa tgtattttt ttaaa
