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Gene information
General information
TC032654
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003953,TC003952 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 27974806..27988658
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032654
    iBeetle screen iB_00643

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    iBeetle screen iB_02741

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_02741

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: % (includes death as pupa, adult)

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032654 RA
      MHTKDFQFK CDTCNKGFF YRKTLNTHI EFYHKGMRL 
VCEFCTREF KNNKQMNIH VRKHYDPDH VVTKFPCPE 
CSKVLSDKY SLKKHVEMI HRGEGGQYV CDICGKRLM 
TNSSWNNHK RIHAGEKNF VCTECGKAF TTGQAFKVH 
IRVHTKEKP YKCTECDKA FSQKGSLTL HLRYHTGER 
PYKCNVCKK QFITNTILK DHKCKGVNN TNAKTYPLD 
DVQDFYCDV CYKVFPSSI KLTKHRTRH RVKTLKCSL 
CPKMFLLPS ELKTHISVQ HEKQYNFFC DLCNYSTYK 
RWSLEVHKI REHTKEYPF KCEVCNKGF VSKVCLTKH 
KEVQHEGRR FTCAFCSKV FRDEGQFKV HTKRHDPNY 
VESRYTCTE CLKVFKTKV RWKDHVKMH FEGRNEYIC 
DICGKSVTT LQSLKDHKT IHTGERNFV CEECGKAFN 
KKQVLQIHK RVHTKEKPF KCTECEKAF SQRSSLIVH 
MRYHTGERP YKYQCEVCS KIFPSYRGF RSHQARHNK 
KHKCPFCEK RYFVPYLLK CHISSTHEN QKKYFCDLC 
DYATYRKPA LGVHKIREH TKEFPFKCE TCNKGFVYK 
LFYTRHNQV EHAGLRYTC EVCSKVFRT EKQFKIHVN 
QHDPNYVEA RYPCTLCLK VFKGKTRWK YHMKMHNEG 
KREYICDVC GKSMSSMQS LKEHRTIHT GEKKFVCET 
CAKAFSTKA LLKTHIRVH TKEKPFKCT ECERAFSQR 
SALVVHLRN KSDPNARFE CEVCHKIFS PWINYRRHT 
YRHRMKTLM CPYCDRIYR TPNEVESHI TRKHDPSQR 
VTCEVCNAS LKKLSLKQH LLSHTKDFP HKCDMCDRG 
FPSKCSLAI HKRAAHEER HLVCEYCAK TFSDNQQFR 
RHVEKHKPD YVTPAFTCD VCFKVLHVE RSFRKHMKR 
HRGEVDSYV CDTCGKSMS TLEGFKIHM RTHTGEKPF 
VCESCGKGF TSKTLLRTH VRVHTKERP YKCTECDKG 
FTQKGSLNV HMRYHTGER PHKCDFCNK NGIPYSDEV 
LGNAFECTI CSKRFLTKR KLGKHILRH REKKHKCPL 
CDRKFISPS EIKTHILYR HDKRKQYFC DHCTFGAPD 
NFTVKQHTI IHHTRDFPF KCDECTKGF ISRQNLAQH 
KKVDHEGVE FVCDLCSKR FKSQANLRR HVAKHDPDY 
TAPSFPCEV CGKVLGKKA SLEYHLKTH KGEASNYVC 
HLCGKSVTS SMSLKYHMD RHAGKRSFV CEVCGKAFT 
TKVLLTTHS RVHTKEKPF KCTECDKAF SQKGTLNIH 
KRVCSSKDL VRRSCPTCY KMVAVGYFT NHLKRHNEK 
THKCPKCDM MFYSNFEVK SHLSSRHDS TKKTFCNVC 
NKPFKNNNS LKVHMVSHT QNFPYKCEV CEKGFTHRY 
LMIDHKVSV HEGVRKVCP FCSKIFINR RLFEMHVAK 
HNSNYVAEA FTCQVCFKV MRTVSGYKI HMKRHKGEL 
RSFMCDICG KSLATREAF LNHQLMHSG EKNHICEFC 
GKAFITKAL LKTHLRVHT KEKPFKCSE CDRAFTQKS 
SLNVHMRYH TGERPYKCA VCSKQFVTK TILKGHACK 
GSGFPVSTF FENCDPLRD EAAGVREQL HPKSRYQYM 
KEYETFSTW RNSNYVDGV TEDILLRYV SDLMEKFAP 
NSVYTKISM LKTLLKKQE NIDISGYGK VFDLMKRKS 
KCYKANKAQ KLTAEEVTR YLLEAPDES FLLEKVITV 
FGIFGACRR EELVTLRAN NIEDTGSLI LVTIENSNN 
RSCRKFVIT EDETPFKGC ALYRKYAAL RPPGLESQR 
FFVDYRNGR CTKQLVGIN KISSIPKKI AKFLKLKNP 
ETYTGHSLR RSGTAVLAE NGVHLLTLK QFRKGRSEE 
FFEDSTSAL TGGTDVVKQ DDAFETVVK SELDSDSD
    CDS sequence
    • >TC032654 RA
      atgcacacc aaggacttc cagttcaag tgcgacact 
tgtaataaa ggttttttc tatcgtaaa actttaaac 
acccacatt gagttctac cataaggga atgcgactc 
gtgtgcgag ttttgcact agagagttt aagaataac 
aagcagatg aacatccat gtgagaaag cattatgat 
cccgaccat gtcgtaact aagtttccc tgtcccgag 
tgttcgaaa gttttgagt gataaatac agtttgaag 
aaacacgtg gaaatgata catcggggt gaaggaggg 
caatatgtg tgtgatatt tgcggaaaa agattgatg 
actaatagt agttggaac aatcataag aggatacat 
gctggggag aagaatttt gtgtgtaca gagtgtggt 
aaagctttt accactgga caggcgttc aaggtacat 
attagggta catacgaag gaaaaaccg tacaagtgt 
acggaatgc gataaggcg ttttcacag aagggtagt 
ttaaccctt catttgcgg taccatact ggtgaaaga 
ccgtacaag tgtaatgtg tgcaagaaa caatttatt 
accaatact attctgaaa gaccacaaa tgcaaaggt 
gttaataat acgaatgcg aaaacatac ccgcttgac 
gacgtccag gatttttat tgcgacgtt tgctacaaa 
gtgttccct tcttccatt aaactgaca aaacataga 
acacgacat cgggtaaaa accctcaaa tgttccttg 
tgccccaaa atgttcctt ttaccttcc gagttaaaa 
acgcatatt tcggtccaa cacgagaag caatacaat 
tttttctgc gacctttgc aactactca acttacaaa 
aggtggtca cttgaagtt cacaaaata cgcgagcac 
acgaaagag taccctttc aaatgcgaa gtttgcaac 
aaaggtttc gtgagtaaa gtgtgtctc actaaacat 
aaagaagtg caacacgaa ggtcgacgc ttcacttgt 
gccttttgc tcgaaagtg tttcgagac gaaggccag 
tttaaagtg cacactaaa cgacacgat cctaactac 
gttgaaagc aggtacact tgtacagaa tgcttgaaa 
gttttcaag actaaagtg cggtggaaa gaccacgtg 
aaaatgcat tttgagggt cgtaatgag tacatttgc 
gatatttgt ggcaaaagt gttactact ttacaaagc 
ttgaaagac cacaagacg atacacaca ggtgaaagg 
aactttgtt tgtgaagaa tgcgggaaa gcttttaac 
aagaagcaa gtgcttcag attcacaaa cgggttcat 
acgaaggaa aaaccgttc aaatgtaca gaatgtgag 
aaggcgttt tctcaaagg agtagtctt atcgtccat 
atgcggtac cacacgggg gaaaggccg tacaaatac 
cagtgcgaa gtctgctcc aaaattttt ccttcgtat 
cgtggattt cgctcacac caagcgcgc cacaacaag 
aaacacaaa tgccctttt tgcgaaaag cgttacttt 
gtcccctac ttactcaaa tgccacatt tcttcaacc 
cacgaaaac cagaaaaaa tatttttgc gacctttgc 
gattatgcg acttatcgc aaaccagcc ttgggagtc 
cacaagatt agagaacac acgaaagag tttcccttc 
aaatgcgag acttgcaac aagggcttt gtgtacaaa 
ttgttttac acaagacat aaccaagtc gaacacgca 
ggcttgaga tacacttgt gaagtttgc tcaaaagta 
ttcagaact gaaaagcaa ttcaaaatt catgtaaac 
caacacgat ccaaattat gttgaagct aggtaccct 
tgtactctg tgcttgaag gttttcaaa ggcaaaacg 
cggtggaaa taccacatg aaaatgcac aacgaaggg 
aaaagggag tacatttgc gatgtttgt ggcaagagt 
atgtcaagt atgcaaagt ttgaaggaa catcggact 
atacatacg ggcgaaaaa aaatttgtg tgcgagact 
tgtgccaaa gctttcagt accaaagcg ttactgaag 
actcacata agggttcac accaaggag aagcccttc 
aagtgcacg gaatgcgaa agggcgttc tctcaaagg 
agcgctctt gtggtacat ttaaggaac aagtcggat 
cctaatgcc cgttttgag tgcgaagtt tgccacaaa 
attttttct ccttggatc aactacagg agacatacc 
tacagacac agaatgaaa acactgatg tgtccttat 
tgtgaccgc atataccgg actccaaac gaagtcgaa 
agtcacata actcgcaaa catgaccct tctcagaga 
gtcacttgt gaggtgtgc aacgcttct ttgaaaaaa 
ctctcactt aagcaacac ctcttaagt cacaccaag 
gattttccg cacaaatgc gatatgtgt gatagaggt 
ttcccctct aaatgttcc ctagcgatc cacaagcga 
gcggctcat gaagaacgt catctggtg tgtgaatat 
tgcgccaaa accttctca gataaccaa caatttcgg 
cgacatgtc gagaagcac aaacctgat tacgtcact 
cctgccttc acatgtgat gtgtgcttc aaggtcttg 
cacgttgag agatctttc aggaagcat atgaaaagg 
cataggggt gaagttgat agttatgtg tgcgataca 
tgcggaaaa agtatgtcg actttggaa ggttttaaa 
attcatatg agaacacac accggagag aaaccattt 
gtttgtgag tcgtgtggt aagggtttt acttcgaaa 
accttgctt agaacacat gttagagtt cacaccaag 
gaacgcccg tataaatgt acagaatgc gataaaggt 
tttactcaa aagggcagt ttgaacgtc catatgcga 
taccacacc ggcgaaagg ccccacaag tgcgatttc 
tgcaacaag aatggcatt ccatactcg gatgaagtt 
ctcggaaac gcttttgag tgcaccatt tgttcaaaa 
agattcctc accaaacga aaactaggc aaacacata 
ttgcgccat cgagaaaaa aaacacaag tgtcctctt 
tgcgacaga aaatttata tctccttca gaaatcaag 
actcacatc ttatacaga cacgacaaa cgtaaacag 
tatttctgc gatcattgt actttcgga gctcctgat 
aacttcaca gttaaacaa cacacaatt atccatcac 
actagagat ttccccttt aagtgtgac gagtgcacc 
aaaggcttc atcagtaga caaaacctg gctcagcac 
aagaaagtc gaccacgaa ggtgtggaa tttgtgtgt 
gacttgtgc tcgaaaaga ttcaagagt caggcaaat 
ctacgaaga catgtcgct aaacacgat cctgattat 
accgctcct tcatttcca tgtgaagtg tgtggaaaa 
gttttgggc aagaaagct tccttagaa tatcatttg 
aaaacgcac aaaggcgag gctagtaat tatgtttgt 
cacttgtgc gggaaaagt gtcacctct tcaatgagt 
cttaagtac catatggat agacatgca gggaagaga 
tcgtttgtt tgtgaagtg tgtggtaag gcatttacc 
acgaaagta cttcttacc acacactca cgcgtacac 
acgaaggaa aaaccgttc aagtgtact gaatgtgac 
aaggcgttt tcccagaag ggtactctc aacatccac 
aagagggtg tgctcatct aaagattta gtccgccgt 
tcgtgtcct acctgttac aagatggtt gctgttggg 
tattttaca aaccatctt aaaagacat aacgaaaag 
actcacaaa tgtcccaaa tgtgacatg atgttttat 
tcgaacttt gaggtgaag tcgcattta tcaagtcga 
catgactct acgaagaaa accttttgt aacgtttgt 
aacaaaccg ttcaaaaac aataactcg ctaaaagta 
cacatggtc tctcatacg caaaatttc ccttacaaa 
tgcgaagtg tgcgaaaaa ggcttcact catagatac 
ctaatgatc gaccacaag gtgtcggtt cacgaagga 
gttcgaaaa gtgtgtcct ttttgctca aaaatattt 
ataaatcgg agattattt gaaatgcac gtggcgaag 
cacaattcc aactacgtg gctgaagct ttcacgtgt 
caagtgtgc ttcaaggtg atgcgtact gtttctggg 
tacaaaata cacatgaaa aggcataaa ggtgagctg 
cgaagcttc atgtgtgac atttgtggg aaaagcttg 
gccacaaga gaagctttt ctaaatcat cagttgatg 
cactctggt gagaagaac cacatttgc gagttttgt 
ggtaaggct ttcattacg aaagctttg ctgaaaacc 
cacttaagg gtgcatacg aaggagaag ccgttcaag 
tgttcagag tgtgataga gcgtttact cagaagagt 
tcacttaat gttcacatg cggtaccat accggggaa 
aggccttac aagtgtgcc gtgtgtagt aaacagttt 
gttaccaaa actattcta aagggccat gcctgtaaa 
ggctcaggg tttcctgtg tcaacattt ttcgaaaat 
tgcgatccc ttacgggat gaagcagct ggagtaaga 
gagcagtta caccctaaa tcaaggtat cagtatatg 
aaagaatac gagactttt tcgacgtgg cgtaacagt 
aattatgtt gatggtgta accgaggat atcttgcta 
cgttatgta tccgactta atggaaaag tttgcaccg 
aacagcgtc tacaccaaa atatccatg ttaaaaaca 
ttgttaaag aaacaagaa aatattgat atttctggt 
tatgggaaa gttttcgat ttgatgaag cgaaaatcg 
aaatgttac aaagcaaat aaagcacaa aagcttaca 
gcggaagaa gtgacgaga tatctactg gaagctcca 
gacgagagt ttcttgctt gaaaaagtt atcacagta 
tttggaatt tttggcgct tgtcgacgc gaggagctc 
gttactttg cgcgcgaat aatattgaa gacactggt 
agtcttata cttgtgaca atagaaaat tcaaataat 
cggtcttgc cgaaagttt gtaataact gaggacgag 
acacctttt aaaggttgt gctttgtac aggaaatac 
gcagcactt agacctccc gggttggag agtcagcgc 
ttttttgtg gactaccgg aacggtaga tgcacaaaa 
cagttagta ggtattaac aaaatttca agtattccg 
aaaaaaatc gccaagttc ttaaaactg aaaaatcct 
gaaacgtac acgggtcat tctttgagg agatctggg 
actgctgtg ttggccgaa aatggggtt cacctcctg 
actctgaaa cagtttcgt aaaggcagg tcagaggag 
ttttttgaa gacagtact tcagcactt acaggtggt 
actgacgtt gtaaaacaa gacgatgct tttgaaacc 
gtagttaag tctgaattg gatagcgat tcagactaa
    mRNA sequence
    • >TC032654 RA
      cgcccacat aactagaat gcacaccaa ggacttcca 
gttcaagtg cgacacttg taataaagg ttttttcta 
tcgtaaaac tttaaacac ccacattga gttctacca 
taagggaat gcgactcgt gtgcgagtt ttgcactag 
agagtttaa gaataacaa gcagatgaa catccatgt 
gagaaagca ttatgatcc cgaccatgt cgtaactaa 
gtttccctg tcccgagtg ttcgaaagt tttgagtga 
taaatacag tttgaagaa acacgtgga aatgataca 
tcggggtga aggagggca atatgtgtg tgatatttg 
cggaaaaag attgatgac taatagtag ttggaacaa 
tcataagag gatacatgc tggggagaa gaattttgt 
gtgtacaga gtgtggtaa agcttttac cactggaca 
ggcgttcaa ggtacatat tagggtaca tacgaagga 
aaaaccgta caagtgtac ggaatgcga taaggcgtt 
ttcacagaa gggtagttt aacccttca tttgcggta 
ccatactgg tgaaagacc gtacaagtg taatgtgtg 
caagaaaca atttattac caatactat tctgaaaga 
ccacaaatg caaaggtgt taataatac gaatgcgaa 
aacataccc gcttgacga cgtccagga tttttattg 
cgacgtttg ctacaaagt gttcccttc ttccattaa 
actgacaaa acatagaac acgacatcg ggtaaaaac 
cctcaaatg ttccttgtg ccccaaaat gttcctttt 
accttccga gttaaaaac gcatatttc ggtccaaca 
cgagaagca atacaattt tttctgcga cctttgcaa 
ctactcaac ttacaaaag gtggtcact tgaagttca 
caaaatacg cgagcacac gaaagagta ccctttcaa 
atgcgaagt ttgcaacaa aggtttcgt gagtaaagt 
gtgtctcac taaacataa agaagtgca acacgaagg 
tcgacgctt cacttgtgc cttttgctc gaaagtgtt 
tcgagacga aggccagtt taaagtgca cactaaacg 
acacgatcc taactacgt tgaaagcag gtacacttg 
tacagaatg cttgaaagt tttcaagac taaagtgcg 
gtggaaaga ccacgtgaa aatgcattt tgagggtcg 
taatgagta catttgcga tatttgtgg caaaagtgt 
tactacttt acaaagctt gaaagacca caagacgat 
acacacagg tgaaaggaa ctttgtttg tgaagaatg 
cgggaaagc ttttaacaa gaagcaagt gcttcagat 
tcacaaacg ggttcatac gaaggaaaa accgttcaa 
atgtacaga atgtgagaa ggcgttttc tcaaaggag 
tagtcttat cgtccatat gcggtacca cacggggga 
aaggccgta caaatacca gtgcgaagt ctgctccaa 
aatttttcc ttcgtatcg tggatttcg ctcacacca 
agcgcgcca caacaagaa acacaaatg ccctttttg 
cgaaaagcg ttactttgt cccctactt actcaaatg 
ccacatttc ttcaaccca cgaaaacca gaaaaaata 
tttttgcga cctttgcga ttatgcgac ttatcgcaa 
accagcctt gggagtcca caagattag agaacacac 
gaaagagtt tcccttcaa atgcgagac ttgcaacaa 
gggctttgt gtacaaatt gttttacac aagacataa 
ccaagtcga acacgcagg cttgagata cacttgtga 
agtttgctc aaaagtatt cagaactga aaagcaatt 
caaaattca tgtaaacca acacgatcc aaattatgt 
tgaagctag gtacccttg tactctgtg cttgaaggt 
tttcaaagg caaaacgcg gtggaaata ccacatgaa 
aatgcacaa cgaagggaa aagggagta catttgcga 
tgtttgtgg caagagtat gtcaagtat gcaaagttt 
gaaggaaca tcggactat acatacggg cgaaaaaaa 
atttgtgtg cgagacttg tgccaaagc tttcagtac 
caaagcgtt actgaagac tcacataag ggttcacac 
caaggagaa gcccttcaa gtgcacgga atgcgaaag 
ggcgttctc tcaaaggag cgctcttgt ggtacattt 
aaggaacaa gtcggatcc taatgcccg ttttgagtg 
cgaagtttg ccacaaaat tttttctcc ttggatcaa 
ctacaggag acataccta cagacacag aatgaaaac 
actgatgtg tccttattg tgaccgcat ataccggac 
tccaaacga agtcgaaag tcacataac tcgcaaaca 
tgacccttc tcagagagt cacttgtga ggtgtgcaa 
cgcttcttt gaaaaaact ctcacttaa gcaacacct 
cttaagtca caccaagga ttttccgca caaatgcga 
tatgtgtga tagaggttt cccctctaa atgttccct 
agcgatcca caagcgagc ggctcatga agaacgtca 
tctggtgtg tgaatattg cgccaaaac cttctcaga 
taaccaaca atttcggcg acatgtcga gaagcacaa 
acctgatta cgtcactcc tgccttcac atgtgatgt 
gtgcttcaa ggtcttgca cgttgagag atctttcag 
gaagcatat gaaaaggca taggggtga agttgatag 
ttatgtgtg cgatacatg cggaaaaag tatgtcgac 
tttggaagg ttttaaaat tcatatgag aacacacac 
cggagagaa accatttgt ttgtgagtc gtgtggtaa 
gggttttac ttcgaaaac cttgcttag aacacatgt 
tagagttca caccaagga acgcccgta taaatgtac 
agaatgcga taaaggttt tactcaaaa gggcagttt 
gaacgtcca tatgcgata ccacaccgg cgaaaggcc 
ccacaagtg cgatttctg caacaagaa tggcattcc 
atactcgga tgaagttct cggaaacgc ttttgagtg 
caccatttg ttcaaaaag attcctcac caaacgaaa 
actaggcaa acacatatt gcgccatcg agaaaaaaa 
acacaagtg tcctctttg cgacagaaa atttatatc 
tccttcaga aatcaagac tcacatctt atacagaca 
cgacaaacg taaacagta tttctgcga tcattgtac 
tttcggagc tcctgataa cttcacagt taaacaaca 
cacaattat ccatcacac tagagattt cccctttaa 
gtgtgacga gtgcaccaa aggcttcat cagtagaca 
aaacctggc tcagcacaa gaaagtcga ccacgaagg 
tgtggaatt tgtgtgtga cttgtgctc gaaaagatt 
caagagtca ggcaaatct acgaagaca tgtcgctaa 
acacgatcc tgattatac cgctccttc atttccatg 
tgaagtgtg tggaaaagt tttgggcaa gaaagcttc 
cttagaata tcatttgaa aacgcacaa aggcgaggc 
tagtaatta tgtttgtca cttgtgcgg gaaaagtgt 
cacctcttc aatgagtct taagtacca tatggatag 
acatgcagg gaagagatc gtttgtttg tgaagtgtg 
tggtaaggc atttaccac gaaagtact tcttaccac 
acactcacg cgtacacac gaaggaaaa accgttcaa 
gtgtactga atgtgacaa ggcgttttc ccagaaggg 
tactctcaa catccacaa gagggtgtg ctcatctaa 
agatttagt ccgccgttc gtgtcctac ctgttacaa 
gatggttgc tgttgggta ttttacaaa ccatcttaa 
aagacataa cgaaaagac tcacaaatg tcccaaatg 
tgacatgat gttttattc gaactttga ggtgaagtc 
gcatttatc aagtcgaca tgactctac gaagaaaac 
cttttgtaa cgtttgtaa caaaccgtt caaaaacaa 
taactcgct aaaagtaca catggtctc tcatacgca 
aaatttccc ttacaaatg cgaagtgtg cgaaaaagg 
cttcactca tagatacct aatgatcga ccacaaggt 
gtcggttca cgaaggagt tcgaaaagt gtgtccttt 
ttgctcaaa aatatttat aaatcggag attatttga 
aatgcacgt ggcgaagca caattccaa ctacgtggc 
tgaagcttt cacgtgtca agtgtgctt caaggtgat 
gcgtactgt ttctgggta caaaataca catgaaaag 
gcataaagg tgagctgcg aagcttcat gtgtgacat 
ttgtgggaa aagcttggc cacaagaga agcttttct 
aaatcatca gttgatgca ctctggtga gaagaacca 
catttgcga gttttgtgg taaggcttt cattacgaa 
agctttgct gaaaaccca cttaagggt gcatacgaa 
ggagaagcc gttcaagtg ttcagagtg tgatagagc 
gtttactca gaagagttc acttaatgt tcacatgcg 
gtaccatac cggggaaag gccttacaa gtgtgccgt 
gtgtagtaa acagtttgt taccaaaac tattctaaa 
gggccatgc ctgtaaagg ctcagggtt tcctgtgtc 
aacattttt cgaaaattg cgatccctt acgggatga 
agcagctgg agtaagaga gcagttaca ccctaaatc 
aaggtatca gtatatgaa agaatacga gactttttc 
gacgtggcg taacagtaa ttatgttga tggtgtaac 
cgaggatat cttgctacg ttatgtatc cgacttaat 
ggaaaagtt tgcaccgaa cagcgtcta caccaaaat 
atccatgtt aaaaacatt gttaaagaa acaagaaaa 
tattgatat ttctggtta tgggaaagt tttcgattt 
gatgaagcg aaaatcgaa atgttacaa agcaaataa 
agcacaaaa gcttacagc ggaagaagt gacgagata 
tctactgga agctccaga cgagagttt cttgcttga 
aaaagttat cacagtatt tggaatttt tggcgcttg 
tcgacgcga ggagctcgt tactttgcg cgcgaataa 
tattgaaga cactggtag tcttatact tgtgacaat 
agaaaattc aaataatcg gtcttgccg aaagtttgt 
aataactga ggacgagac accttttaa aggttgtgc 
tttgtacag gaaatacgc agcacttag acctcccgg 
gttggagag tcagcgctt ttttgtgga ctaccggaa 
cggtagatg cacaaaaca gttagtagg tattaacaa 
aatttcaag tattccgaa aaaaatcgc caagttctt 
aaaactgaa aaatcctga aacgtacac gggtcattc 
tttgaggag atctgggac tgctgtgtt ggccgaaaa 
tggggttca cctcctgac tctgaaaca gtttcgtaa 
aggcaggtc agaggagtt ttttgaaga cagtacttc 
agcacttac aggtggtac tgacgttgt aaaacaaga 
cgatgcttt tgaaaccgt agttaagtc tgaattgga 
tagcgattc agactaatt tgttgtaat ttgaataaa 
tgtattttt ttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY