Status
Please wait ...
Gene information
General information
TC003922 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid26: 634328..648245
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003922
    iBeetle screen iB_00639
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003922 RA
      MAGTKDLLL SEISSPVKD SESKVTVVG VGQVGMACV 
      FSILAQNVS HDVVLIDCV EDKLKGELL DLQHGSLFL 
      KNPRINASS DYAASAGSR LCIVTAGVR QREGESRLD 
      LVQRNTDVL KNIIPKLVK YSPNTVLLI VSNPVDILT 
      YVAWRLSGL PKHRVLGSG TNLDSSRFR FLMSQKLGV 
      APSSCHGWI IGEHGDSSV AVWSGVNVA GVRLREINP 
      SMGTQQDPE RWDQVHKEV VNSAYEIIR LKGYTNWAI 
      GLSVASLAQ SILNNSHNV HAVSVNVKG LHGITQEVY 
      LSLPSVLGE NGVRSIVNQ CLTEDEKQR LLKSATTLD 
      DIQKTINL
    CDS sequence
    • >TC003922 RA
      atggccgga accaaagat ctcctcctt tccgaaatt 
      tcgtcacca gtgaaagac agtgaatcg aaagtcact 
      gtcgttggt gttggccaa gtcggaatg gcttgtgtt 
      ttcagtatt ttggcacaa aatgtatca cacgatgtt 
      gtgcttatt gactgcgtg gaggacaaa ctcaaggga 
      gagctgttg gatcttcaa cacggttca ctttttctc 
      aaaaatcca agaatcaat gctagttca gactatgca 
      gcctctgct ggttctcgc ctttgcatt gtaacagct 
      ggagtccgc caacgtgag ggcgaatcc cgtctggat 
      ttagtccaa cgcaacact gacgtcctc aaaaatatt 
      atcccgaaa ctagtcaaa tactcgcca aacacagtt 
      ttactcatc gtaagcaac cctgtcgat atcctcacc 
      tacgtcgct tggagactt agcggatta ccaaagcac 
      agggttctg ggttcagga accaatctg gattcgtcc 
      agattcaga tttttgatg tcgcaaaag ctgggagtg 
      gctccgagc agttgccat ggctggatt ataggcgaa 
      catggcgat tccagcgtg gcggtgtgg tccggtgtg 
      aatgtagcc ggtgtccgc ttacgcgaa attaatccg 
      agtatgggg acgcaacag gatccggaa cgttgggac 
      caggtgcac aaggaagtg gttaacagt gcctatgag 
      attatcagg ctgaagggt tacaccaat tgggcgatt 
      ggactgagt gttgcctca ttggcgcaa agtatactc 
      aacaattcg cacaatgtt catgctgtt tctgttaat 
      gttaagggc cttcacggc attactcag gaagtctac 
      ttatcgctt ccatcagtt ttgggcgaa aacggtgtt 
      cggtcaatt gtaaatcaa tgtttgacc gaagatgag 
      aaacaacgc ttgttaaaa tcagcgaca acacttgac 
      gatattcaa aaaaccatt aatttgtag
    mRNA sequence
    • >TC003922 RA
      gggctccta ttttcattg tacttctgc aaattgtct 
      tcgacaggg gacatgcgc atcgaccaa attttagtc 
      cgtataaga ggtcacctt gctccagtc tgccgctaa 
      gttttaagc ttgtaaagt ggtcaagtt gtttttacc 
      gacatggcc ggaaccaaa gatctcctc ctttccgaa 
      atttcgtca ccagtgaaa gacagtgaa tcgaaagtc 
      actgtcgtt ggtgttggc caagtcgga atggcttgt 
      gttttcagt attttggca caaaatgta tcacacgat 
      gttgtgctt attgactgc gtggaggac aaactcaag 
      ggagagctg ttggatctt caacacggt tcacttttt 
      ctcaaaaat ccaagaatc aatgctagt tcagactat 
      gcagcctct gctggttct cgcctttgc attgtaaca 
      gctggagtc cgccaacgt gagggcgaa tcccgtctg 
      gatttagtc caacgcaac actgacgtc ctcaaaaat 
      attatcccg aaactagtc aaatactcg ccaaacaca 
      gttttactc atcgtaagc aaccctgtc gatatcctc 
      acctacgtc gcttggaga cttagcgga ttaccaaag 
      cacagggtt ctgggttca ggaaccaat ctggattcg 
      tccagattc agatttttg atgtcgcaa aagctggga 
      gtggctccg agcagttgc catggctgg attataggc 
      gaacatggc gattccagc gtggcggtg tggtccggt 
      gtgaatgta gccggtgtc cgcttacgc gaaattaat 
      ccgagtatg gggacgcaa caggatccg gaacgttgg 
      gaccaggtg cacaaggaa gtggttaac agtgcctat 
      gagattatc aggctgaag ggttacacc aattgggcg 
      attggactg agtgttgcc tcattggcg caaagtata 
      ctcaacaat tcgcacaat gttcatgct gtttctgtt 
      aatgttaag ggccttcac ggcattact caggaagtc 
      tacttatcg cttccatca gttttgggc gaaaacggt 
      gttcggtca attgtaaat caatgtttg accgaagat 
      gagaaacaa cgcttgtta aaatcagcg acaacactt 
      gacgatatt caaaaaacc attaatttg tagccacat 
      aaccaacaa ctaactttt ttgttagat tagtttaga 
      tcgccctag ttttgctgt atttttagt tgtaagcgt 
      ttgattggg tggttcttt aggggcacg tggggatga 
      atattgaca cgtttttgt cgctgcctt gcgtgttgg 
      gcctgaatg gcgtcaatc aagtggtga aaataaatt 
      tgcaaccgg atgaactaa agttgttta aagcttcgg 
      ccgaagcga tggaagccg ttcagaaga aaattgtat 
      tttaatatt tctgatgtg tgaataaat ttcggtgtt 
      tttc