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Gene information
General information
TC003916 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid26: 251022..283506
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003916
    iBeetle screen iB_00638
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 2

    egg chamber mostly not present - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    antenna orientation bent - Number of animals showing the phenotype: 8

    wings orientation irregular - Number of animals showing the phenotype: 8

    Adult morphology
    antenna bent - Number of animals showing the phenotype: 6


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003916 RA
      MQDQIVVDG QNHLSSGTN SGLPITGSP IVLKMNDVP 
      QLSSVGLLE LAHREYQAG DYENAERHC MQLWRQDQT 
      NTGVLLLLS SIHFQCRRL DKSAHFSTL AIKQNPLLA 
      EAYSNLGNV YKERSQLQE ALDNYRHAV RLKPDFIDG 
      YINLAAALV AAGDMEQAV QAYITALQY NPDLYCVRS 
      DLGNLLKAL GRLDEAKAC YLKAIETRP DFAVAWSNL 
      GCVFNAQGE IWLAIHHFE KAVGLDPNF LDAYINLGN 
      VLKEARIFD RAVAAYLRA LNLSPNNAV VHGNLACVY 
      YEQGLIDLA IDTYRRAIE LQPNFPDAY CNLANALKE 
      KGQVAEAEE CYNTALRLC PSHADSLNN LANIKREQG 
      YIEEATRLY LKALEVFPE FAAAHSNLA SVLQQQGKL 
      NEALMHYKE AIRIQPTFA DAYSNMGNT LKEMQDVSG 
      ALQCYTRAI QINPAFADA HSNLASIHK DSGNIPEAI 
      QSYRTALKL KPDFPDAYC NLAHCLQIV CDWTDYDAR 
      MKKLVSIVA DQLEKNRLP SVHPHHSML YPLSHEFRK 
      AIAARHANL CLEKIHVLH KHPYKFTTE LKGRLRVGY 
      VSSDFGNHP TSHLMQSVP GLHDRSRVE IFCYALSQD 
      DGTTFRSKI SREAEHFID LSQIPCNGK AADRIHADG 
      IHILVNMNG YTKGARNEI FALKPAPVQ VMWLGYPGT 
      SGASFMDYL VTDAVTSPV ELASQYSEK LAYMPYTYF 
      VGDHRQMFP HLKERLIVS DKNNSTNSN LADNVAIIN 
      ATDLSPLVE STEVKEIKE VVKAAKMVE ISLKVAELP 
      TTTPIENMI ASGQVQTSL NGVVVQNGL ATTQTNNKA 
      ATGEEVPQN IVITTRQQY GLPDDAVVY CNFNQLYKI 
      DPMTLHMWV NILKAVPNS VLWLLRFPA VGEPNLQAT 
      AQQLGLPPG RILFSNVAA KEEHVRRGQ LADVCLDTP 
      LCNGHTTSM DVLWTGTPV VTFPGETLA SRVAASQLA 
      TLGCPELIA RSRQEYQEI AIKLGTDKE YLKAMRHKV 
      WAARTTSPL FDCKQYAQG LELLYCKMW ERFAAGLKP 
      DHITDTTAK K
    • >TC003916 RB
      MRSLRFGDF FLSQPACYL KAIETRPDF AVAWSNLGC 
      VFNAQGEIW LAIHHFEKA VGLDPNFLD AYINLGNVL 
      KEARIFDRA VAAYLRALN LSPNNAVVH GNLACVYYE 
      QGLIDLAID TYRRAIELQ PNFPDAYCN LANALKEKG 
      QVAEAEECY NTALRLCPS HADSLNNLA NIKREQGYI 
      EEATRLYLK ALEVFPEFA AAHSNLASV LQQQGKLNE 
      ALMHYKEAI RIQPTFADA YSNMGNTLK EMQDVSGAL 
      QCYTRAIQI NPAFADAHS NLASIHKDS GNIPEAIQS 
      YRTALKLKP DFPDAYCNL AHCLQIVCD WTDYDARMK 
      KLVSIVADQ LEKNRLPSV HPHHSMLYP LSHEFRKAI 
      AARHANLCL EKIHVLHKH PYKFTTELK GRLRVGYVS 
      SDFGNHPTS HLMQSVPGL HDRSRVEIF CYALSQDDG 
      TTFRSKISR EAEHFIDLS QIPCNGKAA DRIHADGIH 
      ILVNMNGYT KGARNEIFA LKPAPVQVM WLGYPGTSG 
      ASFMDYLVT DAVTSPVEL ASQYSEKLA YMPYTYFVG 
      DHRQMFPHL KERLIVSDK NNSTNSNLA DNVAIINAT 
      DLSPLVEST EVKEIKEVV KAAKMVEIS LKVAELPTT 
      TPIENMIAS GQVQTSLNG VVVQNGLAT TQTNNKAAT 
      GEEVPQNIV ITTRQQYGL PDDAVVYCN FNQLYKIDP 
      MTLHMWVNI LKAVPNSVL WLLRFPAVG EPNLQATAQ 
      QLGLPPGRI LFSNVAAKE EHVRRGQLA DVCLDTPLC 
      NGHTTSMDV LWTGTPVVT FPGETLASR VAASQLATL 
      GCPELIARS RQEYQEIAI KLGTDKEYL KAMRHKVWA 
      ARTTSPLFD CKQYAQGLE LLYCKMWER FAAGLKPDH 
      ITDTTAKK
    • >TC003916 RC
      MQDQIVVDG QNHLSSGTN SGLPITGSP IVLKMNDVP 
      QLSSVGLLE LAHREYQAG DYENAERHC MQLWRQDQT 
      NTGVLLLLS SIHFQCRRL DKSAHFSTL AIKQNPLLA 
      EAYSNLGNV YKERSQLQE ALDNYRHAV RLKPDFIDG 
      YINLAAALV AAGDMEQAV QAYITALQY NPDLYCVRS 
      DLGNLLKAL GRLDEAKNT ISIYSNLFQ SPKLKNQVK 
      YKFYKA
    CDS sequence
    • >TC003916 RA
      atgcaggac cagattgtg gtcgatggt cagaaccat 
      ctcagttct ggaacaaac agcgggttg cccatcacc 
      ggtagtccc attgtctta aagatgaat gatgttccg 
      cagctttcg tccgttggg cttcttgaa cttgcccat 
      cgtgagtac caagctggg gattatgaa aatgcagag 
      aggcattgt atgcagttg tggagacag gatcagacc 
      aatacgggg gttttactg cttttgtca agcatacat 
      ttccagtgc aggcgttta gacaaatcc gcacatttc 
      agcactttg gcaatcaaa caaaatcca ctcctcgcc 
      gaagcttac agtaatttg ggcaacgtg tacaaagaa 
      agaagtcag ctgcaagaa gcactcgat aattatcga 
      catgcagtg cgtcttaaa ccagatttt attgatgga 
      tacatcaac ttagctgca gctcttgtt gctgcagga 
      gatatggag caagcagtt caagcatac attaccgca 
      ttgcagtac aatccagat ctctactgt gttcgtagc 
      gatttgggt aacctccta aaagcatta ggtcgttta 
      gacgaggcc aaggcgtgt tatttgaaa gctatcgag 
      actagaccg gatttcgcc gtcgcttgg agcaatctt 
      ggatgcgtt tttaacgct caaggggag atctggctc 
      gcgatacat catttcgaa aaagcagtc ggtttggat 
      cccaatttc ttggatgcc tacatcaat ctgggcaat 
      gtgctgaaa gaagcgcga attttcgat cgggcggta 
      gcggcgtat ttgcgggcc ctaaacctc tcgcccaac 
      aatgccgtc gtccacggc aacctggcc tgtgtctac 
      tacgaacag ggtctcatt gacttggcc atcgacacg 
      tatcgaagg gccatcgag ttgcagccc aacttcccc 
      gacgcctac tgcaacctg gcgaacgcc ctcaaggaa 
      aagggccag gtggcggag gccgaagag tgctacaac 
      acagcgctg cgcttgtgc ccttcacat gccgactca 
      ctcaataac ttggccaat atcaaacgc gaacagggt 
      tacatcgag gaggcgact cgactctac ctaaaagca 
      ctggaagtg ttcccagag tttgcagcc gctcacagc 
      aacctcgcc tccgtgtta caacaacag ggtaaacta 
      aacgaagcg ctaatgcat tacaaggag gcgatccgg 
      atccagccg accttcgcc gacgcctac tccaacatg 
      gggaacacc ctgaaggag atgcaggac gtttcgggc 
      gccctccag tgctacact cgggcgatt cagatcaat 
      ccagctttt gccgacgcc cacagcaac ctagcgagc 
      atccacaag gactcggga aacattcca gaagcgata 
      cagtcgtac cgaacggcc ttgaaactc aagccggac 
      tttccagac gcctactgc aacctcgcc cattgtctt 
      caaatcgtc tgtgattgg acggattat gacgccagg 
      atgaagaaa ttggtcagt attgttgcc gaccagctc 
      gagaaaaac cgactacct tcggtccac ccccaccac 
      tcaatgctc tacccccta tcacacgag ttccgtaaa 
      gccatagcg gcacgtcat gccaactta tgcctggaa 
      aaaatccac gtgttgcac aaacacccc tacaaattc 
      accacagag ttaaagggg cggctgaga gtcggttac 
      gtgagcagt gattttggg aaccaccca acctcacac 
      ttgatgcag tcggttccg gggttgcac gaccgctcc 
      cgagtcgag attttctgc tatgcgttg agtcaagac 
      gatgggacg acgttcagg agtaaaata tcgcgcgaa 
      gctgagcat tttatcgat ctatcgcag attccctgc 
      aatgggaag gcggccgat cgcattcat gccgatggt 
      attcatatt ttggtcaat atgaatggg tataccaaa 
      ggcgcccgt aatgagatt ttcgcgctg aagccggcg 
      ccggttcag gtcatgtgg ttggggtat cccgggacc 
      agtggggcg agtttcatg gactatttg gtgacggat 
      gcggtgacg tcgccggtt gagttggcc agtcagtat 
      agcgagaaa ctggcctat atgccttat acttatttt 
      gtgggggat catagacag atgtttccg catcttaag 
      gagagactc atcgtgagt gataagaat aacagtaca 
      aattcgaat ttggccgat aatgtggcc attattaat 
      gcgacggat ttgtcgccg ttggttgaa agtacggag 
      gttaaggaa atcaaggag gtcgtcaag gctgcaaaa 
      atggtggaa atcagtcta aaagtggcc gaactcccc 
      acaacaacc cccattgaa aacatgatc gcctctggc 
      caggtccag acttcactc aacggcgta gtggtccaa 
      aacggcctc gccaccacc caaaccaac aacaaagcc 
      gccaccggt gaagaagtc ccccagaac atcgtaatc 
      acgacccga caacaatac ggactccct gacgacgcc 
      gtcgtctat tgcaatttc aaccaattg tacaaaatc 
      gatccgatg acattgcac atgtgggtc aacatatta 
      aaggctgtg cccaattcc gttctctgg ctgttgcgt 
      ttcccggcc gttggcgag cccaattta caagcaaca 
      gcacagcaa ttggggctt cccccaggc aggattctg 
      ttcagtaat gtggcggct aaagaggag catgtgagg 
      aggggacag ttggcggat gtgtgtttg gatacgccc 
      ttgtgtaat ggacatacc accagtatg gatgttttg 
      tggacgggg acgccggtc gtgaccttc ccgggggaa 
      accctggct tcaagggtg gctgccagt cagttggct 
      acacttggg tgtccggaa ttgatagca cggtccagg 
      caggagtat caagaaatt gctatcaag ctaggaact 
      gataaggaa tatttgaag gcgatgagg cacaaagtt 
      tgggcagct cgcaccaca tcgccgctg tttgactgc 
      aaacagtac gcccaaggt ctggaattg ttgtactgc 
      aaaatgtgg gaacgattc gctgcaggg ctcaagcca 
      gatcatata actgacaca accgcaaaa aaatag
    • >TC003916 RB
      atgcgttca ttgcgtttt ggcgatttt tttctgtcc 
      caacctgcg tgttatttg aaagctatc gagactaga 
      ccggatttc gccgtcgct tggagcaat cttggatgc 
      gtttttaac gctcaaggg gagatctgg ctcgcgata 
      catcatttc gaaaaagca gtcggtttg gatcccaat 
      ttcttggat gcctacatc aatctgggc aatgtgctg 
      aaagaagcg cgaattttc gatcgggcg gtagcggcg 
      tatttgcgg gccctaaac ctctcgccc aacaatgcc 
      gtcgtccac ggcaacctg gcctgtgtc tactacgaa 
      cagggtctc attgacttg gccatcgac acgtatcga 
      agggccatc gagttgcag cccaacttc cccgacgcc 
      tactgcaac ctggcgaac gccctcaag gaaaagggc 
      caggtggcg gaggccgaa gagtgctac aacacagcg 
      ctgcgcttg tgcccttca catgccgac tcactcaat 
      aacttggcc aatatcaaa cgcgaacag ggttacatc 
      gaggaggcg actcgactc tacctaaaa gcactggaa 
      gtgttccca gagtttgca gccgctcac agcaacctc 
      gcctccgtg ttacaacaa cagggtaaa ctaaacgaa 
      gcgctaatg cattacaag gaggcgatc cggatccag 
      ccgaccttc gccgacgcc tactccaac atggggaac 
      accctgaag gagatgcag gacgtttcg ggcgccctc 
      cagtgctac actcgggcg attcagatc aatccagct 
      tttgccgac gcccacagc aacctagcg agcatccac 
      aaggactcg ggaaacatt ccagaagcg atacagtcg 
      taccgaacg gccttgaaa ctcaagccg gactttcca 
      gacgcctac tgcaacctc gcccattgt cttcaaatc 
      gtctgtgat tggacggat tatgacgcc aggatgaag 
      aaattggtc agtattgtt gccgaccag ctcgagaaa 
      aaccgacta ccttcggtc cacccccac cactcaatg 
      ctctacccc ctatcacac gagttccgt aaagccata 
      gcggcacgt catgccaac ttatgcctg gaaaaaatc 
      cacgtgttg cacaaacac ccctacaaa ttcaccaca 
      gagttaaag gggcggctg agagtcggt tacgtgagc 
      agtgatttt gggaaccac ccaacctca cacttgatg 
      cagtcggtt ccggggttg cacgaccgc tcccgagtc 
      gagattttc tgctatgcg ttgagtcaa gacgatggg 
      acgacgttc aggagtaaa atatcgcgc gaagctgag 
      cattttatc gatctatcg cagattccc tgcaatggg 
      aaggcggcc gatcgcatt catgccgat ggtattcat 
      attttggtc aatatgaat gggtatacc aaaggcgcc 
      cgtaatgag attttcgcg ctgaagccg gcgccggtt 
      caggtcatg tggttgggg tatcccggg accagtggg 
      gcgagtttc atggactat ttggtgacg gatgcggtg 
      acgtcgccg gttgagttg gccagtcag tatagcgag 
      aaactggcc tatatgcct tatacttat tttgtgggg 
      gatcataga cagatgttt ccgcatctt aaggagaga 
      ctcatcgtg agtgataag aataacagt acaaattcg 
      aatttggcc gataatgtg gccattatt aatgcgacg 
      gatttgtcg ccgttggtt gaaagtacg gaggttaag 
      gaaatcaag gaggtcgtc aaggctgca aaaatggtg 
      gaaatcagt ctaaaagtg gccgaactc cccacaaca 
      acccccatt gaaaacatg atcgcctct ggccaggtc 
      cagacttca ctcaacggc gtagtggtc caaaacggc 
      ctcgccacc acccaaacc aacaacaaa gccgccacc 
      ggtgaagaa gtcccccag aacatcgta atcacgacc 
      cgacaacaa tacggactc cctgacgac gccgtcgtc 
      tattgcaat ttcaaccaa ttgtacaaa atcgatccg 
      atgacattg cacatgtgg gtcaacata ttaaaggct 
      gtgcccaat tccgttctc tggctgttg cgtttcccg 
      gccgttggc gagcccaat ttacaagca acagcacag 
      caattgggg cttccccca ggcaggatt ctgttcagt 
      aatgtggcg gctaaagag gagcatgtg aggagggga 
      cagttggcg gatgtgtgt ttggatacg cccttgtgt 
      aatggacat accaccagt atggatgtt ttgtggacg 
      gggacgccg gtcgtgacc ttcccgggg gaaaccctg 
      gcttcaagg gtggctgcc agtcagttg gctacactt 
      gggtgtccg gaattgata gcacggtcc aggcaggag 
      tatcaagaa attgctatc aagctagga actgataag 
      gaatatttg aaggcgatg aggcacaaa gtttgggca 
      gctcgcacc acatcgccg ctgtttgac tgcaaacag 
      tacgcccaa ggtctggaa ttgttgtac tgcaaaatg 
      tgggaacga ttcgctgca gggctcaag ccagatcat 
      ataactgac acaaccgca aaaaaatag
    • >TC003916 RC
      atgcaggac cagattgtg gtcgatggt cagaaccat 
      ctcagttct ggaacaaac agcgggttg cccatcacc 
      ggtagtccc attgtctta aagatgaat gatgttccg 
      cagctttcg tccgttggg cttcttgaa cttgcccat 
      cgtgagtac caagctggg gattatgaa aatgcagag 
      aggcattgt atgcagttg tggagacag gatcagacc 
      aatacgggg gttttactg cttttgtca agcatacat 
      ttccagtgc aggcgttta gacaaatcc gcacatttc 
      agcactttg gcaatcaaa caaaatcca ctcctcgcc 
      gaagcttac agtaatttg ggcaacgtg tacaaagaa 
      agaagtcag ctgcaagaa gcactcgat aattatcga 
      catgcagtg cgtcttaaa ccagatttt attgatgga 
      tacatcaac ttagctgca gctcttgtt gctgcagga 
      gatatggag caagcagtt caagcatac attaccgca 
      ttgcagtac aatccagat ctctactgt gttcgtagc 
      gatttgggt aacctccta aaagcatta ggtcgttta 
      gacgaggcc aagaatact atttccatc tactccaat 
      ttatttcaa tctccaaaa ttaaaaaat caagtaaaa 
      tacaaattt tacaaggca taa
    mRNA sequence
    • >TC003916 RA
      tcattatca ttatttatt aatgtcagc aatttttat 
      tgtctaata tactacaga gcctaccga tcgcacctc 
      atcgggcgt gaaattgat aagacgcct tgtggtttt 
      ttgtgctcc gaagctcgt cacagcccg ggaatagtg 
      caaatggtc ccatctggt aaatcccat tgaatcgca 
      aaaaatcgt tgtgggccc cttcgcacc cattgacga 
      ccaaatcca ggtaaataa cggaccatg gaatggaaa 
      tttccactc gcttccacc aatcacacg ccagctcca 
      gcagctcaa agaccaatc aggtcgcag ctgcgcttt 
      gaccacagt tcttcagtt gtctctctt cagcggcgg 
      ccatttgag gccatcaga gaatcgatt gttgtaatt 
      tagagcgtt ttttggtga aaaactgtg gtgaaagtg 
      tgcagaaat aggtcagat agggtgtat gttggtgta 
      acggcgtgc tttaccatg caggaccag attgtggtc 
      gatggtcag aaccatctc agttctgga acaaacagc 
      gggttgccc atcaccggt agtcccatt gtcttaaag 
      atgaatgat gttccgcag ctttcgtcc gttgggctt 
      cttgaactt gcccatcgt gagtaccaa gctggggat 
      tatgaaaat gcagagagg cattgtatg cagttgtgg 
      agacaggat cagaccaat acgggggtt ttactgctt 
      ttgtcaagc atacatttc cagtgcagg cgtttagac 
      aaatccgca catttcagc actttggca atcaaacaa 
      aatccactc ctcgccgaa gcttacagt aatttgggc 
      aacgtgtac aaagaaaga agtcagctg caagaagca 
      ctcgataat tatcgacat gcagtgcgt cttaaacca 
      gattttatt gatggatac atcaactta gctgcagct 
      cttgttgct gcaggagat atggagcaa gcagttcaa 
      gcatacatt accgcattg cagtacaat ccagatctc 
      tactgtgtt cgtagcgat ttgggtaac ctcctaaaa 
      gcattaggt cgtttagac gaggccaag gcgtgttat 
      ttgaaagct atcgagact agaccggat ttcgccgtc 
      gcttggagc aatcttgga tgcgttttt aacgctcaa 
      ggggagatc tggctcgcg atacatcat ttcgaaaaa 
      gcagtcggt ttggatccc aatttcttg gatgcctac 
      atcaatctg ggcaatgtg ctgaaagaa gcgcgaatt 
      ttcgatcgg gcggtagcg gcgtatttg cgggcccta 
      aacctctcg cccaacaat gccgtcgtc cacggcaac 
      ctggcctgt gtctactac gaacagggt ctcattgac 
      ttggccatc gacacgtat cgaagggcc atcgagttg 
      cagcccaac ttccccgac gcctactgc aacctggcg 
      aacgccctc aaggaaaag ggccaggtg gcggaggcc 
      gaagagtgc tacaacaca gcgctgcgc ttgtgccct 
      tcacatgcc gactcactc aataacttg gccaatatc 
      aaacgcgaa cagggttac atcgaggag gcgactcga 
      ctctaccta aaagcactg gaagtgttc ccagagttt 
      gcagccgct cacagcaac ctcgcctcc gtgttacaa 
      caacagggt aaactaaac gaagcgcta atgcattac 
      aaggaggcg atccggatc cagccgacc ttcgccgac 
      gcctactcc aacatgggg aacaccctg aaggagatg 
      caggacgtt tcgggcgcc ctccagtgc tacactcgg 
      gcgattcag atcaatcca gcttttgcc gacgcccac 
      agcaaccta gcgagcatc cacaaggac tcgggaaac 
      attccagaa gcgatacag tcgtaccga acggccttg 
      aaactcaag ccggacttt ccagacgcc tactgcaac 
      ctcgcccat tgtcttcaa atcgtctgt gattggacg 
      gattatgac gccaggatg aagaaattg gtcagtatt 
      gttgccgac cagctcgag aaaaaccga ctaccttcg 
      gtccacccc caccactca atgctctac cccctatca 
      cacgagttc cgtaaagcc atagcggca cgtcatgcc 
      aacttatgc ctggaaaaa atccacgtg ttgcacaaa 
      cacccctac aaattcacc acagagtta aaggggcgg 
      ctgagagtc ggttacgtg agcagtgat tttgggaac 
      cacccaacc tcacacttg atgcagtcg gttccgggg 
      ttgcacgac cgctcccga gtcgagatt ttctgctat 
      gcgttgagt caagacgat gggacgacg ttcaggagt 
      aaaatatcg cgcgaagct gagcatttt atcgatcta 
      tcgcagatt ccctgcaat gggaaggcg gccgatcgc 
      attcatgcc gatggtatt catattttg gtcaatatg 
      aatgggtat accaaaggc gcccgtaat gagattttc 
      gcgctgaag ccggcgccg gttcaggtc atgtggttg 
      gggtatccc gggaccagt ggggcgagt ttcatggac 
      tatttggtg acggatgcg gtgacgtcg ccggttgag 
      ttggccagt cagtatagc gagaaactg gcctatatg 
      ccttatact tattttgtg ggggatcat agacagatg 
      tttccgcat cttaaggag agactcatc gtgagtgat 
      aagaataac agtacaaat tcgaatttg gccgataat 
      gtggccatt attaatgcg acggatttg tcgccgttg 
      gttgaaagt acggaggtt aaggaaatc aaggaggtc 
      gtcaaggct gcaaaaatg gtggaaatc agtctaaaa 
      gtggccgaa ctccccaca acaaccccc attgaaaac 
      atgatcgcc tctggccag gtccagact tcactcaac 
      ggcgtagtg gtccaaaac ggcctcgcc accacccaa 
      accaacaac aaagccgcc accggtgaa gaagtcccc 
      cagaacatc gtaatcacg acccgacaa caatacgga 
      ctccctgac gacgccgtc gtctattgc aatttcaac 
      caattgtac aaaatcgat ccgatgaca ttgcacatg 
      tgggtcaac atattaaag gctgtgccc aattccgtt 
      ctctggctg ttgcgtttc ccggccgtt ggcgagccc 
      aatttacaa gcaacagca cagcaattg gggcttccc 
      ccaggcagg attctgttc agtaatgtg gcggctaaa 
      gaggagcat gtgaggagg ggacagttg gcggatgtg 
      tgtttggat acgcccttg tgtaatgga cataccacc 
      agtatggat gttttgtgg acggggacg ccggtcgtg 
      accttcccg ggggaaacc ctggcttca agggtggct 
      gccagtcag ttggctaca cttgggtgt ccggaattg 
      atagcacgg tccaggcag gagtatcaa gaaattgct 
      atcaagcta ggaactgat aaggaatat ttgaaggcg 
      atgaggcac aaagtttgg gcagctcgc accacatcg 
      ccgctgttt gactgcaaa cagtacgcc caaggtctg 
      gaattgttg tactgcaaa atgtgggaa cgattcgct 
      gcagggctc aagccagat catataact gacacaacc 
      gcaaaaaaa tagaaacaa attgatttt tgtgggact 
      gatatttac tcatctaaa ttattttgt aatttctag 
      cttttggac agagttgtg tgaagactc cagatcatg 
      atgttaatt tagttcaag ttgtatgca gttattatt 
      gagttgtga aatgtgaca taagatcgc tgtgttgta 
      ttataatat atttcaaca agttca
    • >TC003916 RB
      tttgagcta aaatctctg gtcttttgc tagattttg 
      ttaatatgt gatgggaaa tcgacgaaa atggcgatt 
      tttgggcgt tttacaaag tatttcact cagaagtgt 
      aattaattc gagaaattt tttaaaaat aaatcgaaa 
      gaacactaa atttggtct aattttaac cttgttcga 
      gcgtttaag cacattttt acgctgaaa acccgggag 
      gtaattgtt taacgtata ttataaaaa atataattg 
      aaaaatggt tgcattggg actattttt taaaatgct 
      ttaccgctg aataccttt ttaagaaaa aacatttac 
      gtgaaattt taaacacag aattgagtt taaacacag 
      agaagctca ataattccg gcaatttca ttaaaaatg 
      caagttttg tgaaatgca attattttg agtaaaaat 
      aaatcgaaa agttaaaaa ttgattaga catttgtgt 
      tattaaacg atgttgcgt tgtattttg taataattt 
      ttagcaaaa aattcagtt cttggcgaa aattttcga 
      aacactgaa ttgggttgc aggtgatat tatttatcg 
      ttgatgtgt tttattttg tacattttt atgcagaaa 
      ctaaataaa aaaatcgaa tttcgtcga aaatgcggc 
      tgtttttgt cattccttt gcactttaa aaagtttct 
      tgagtttaa attaattat agttttttt tgagcattt 
      taatacatt ttttagcca attatttta cggagcatt 
      gtcaaaaat aagctgaaa actcacaaa aagttgttt 
      tggtacgtt tttatgcat aaactaatt aatttcgcg 
      agattttgt aaaaaatgg tgcagtttt tgtcattta 
      ttgcacttc ttactagta gttttaaaa tgaattgca 
      ttagcttca tttgacatc aggttaaaa agtgccgaa 
      gtttgtctt aatgttact tctttatta cttttttta 
      ttataattt ttctttctc tgggtactg cgaaattat 
      catcaataa aattttctg tttataaaa cgtcaaaag 
      aatctggaa ataaatcag ttgtttcgc gaataaagt 
      tatcatgag ctgttttgc gtgaattta atttcaagc 
      tacatttgc tgtaatagc tttttctag aggaatgct 
      tgtaaatta agctaaatt tttgcattt aaatagggc 
      gttagggag catttatta aaaaaagtt taatattaa 
      aataacaac tttcggagg tttttgatt tttgtttat 
      taataacga agatattta ccgggaacg tctcagttg 
      agacactgt tttttaatt ttgttgctt ttgttattt 
      taattatcg caaaaaaga gtttcacca aaatatcgg 
      tatcaatgt ttttttttt cctaaagaa gcttattta 
      catcaacta gacagtact ttaaggaaa attcgaact 
      ctgaaattg gaagcataa ataaataat cagttagtt 
      caatttttt ttgctcatt tagctgaga atttttacc 
      agttttctt cgcactgtt gtatcttat gccttggga 
      agatgaagc ggtaggcgt ataaaaata aatatttca 
      ggagagttg atatttcct ttgggtacg cggagtgtc 
      tttggattc aggtgcgat caaatggga acaaaatct 
      gcttttgtg tcagttttg ttccaaatg gttgtgagc 
      cagctttca gggacaccc gtttcagtg aaaatgacg 
      gtgaacatt gtaaataag ttgtttctt ttaagttta 
      ggcccggtg gcattgagt ttctggttt agagagtgg 
      gaatagctt tggtgattt tctgtttgt ggccgccta 
      taattgaca aatgagaat gctgactaa tggtattac 
      tgttgctgt acaataata caggtatgt ggctggtgt 
      cccgtgcgc ctccctgga atcacactg gtaaaacta 
      tgggcttag tctcaggta atgtacgca agcgaacaa 
      gatgtttct ttgttttta ggtattgga gtgaggcct 
      ctctgtgcg tcaattgct tgcttgcgt aatgagttt 
      tcttctttt tttcgaccg tttctatgt cgattttaa 
      ggtgcgtaa gttgtgcgc tggcgtatt ttttcatct 
      cagtatgcg ttcattgcg ttttggcga tttttttct 
      gtcccaacc tgcgtgtta tttgaaagc tatcgagac 
      tagaccgga tttcgccgt cgcttggag caatcttgg 
      atgcgtttt taacgctca aggggagat ctggctcgc 
      gatacatca tttcgaaaa agcagtcgg tttggatcc 
      caatttctt ggatgccta catcaatct gggcaatgt 
      gctgaaaga agcgcgaat tttcgatcg ggcggtagc 
      ggcgtattt gcgggccct aaacctctc gcccaacaa 
      tgccgtcgt ccacggcaa cctggcctg tgtctacta 
      cgaacaggg tctcattga cttggccat cgacacgta 
      tcgaagggc catcgagtt gcagcccaa cttccccga 
      cgcctactg caacctggc gaacgccct caaggaaaa 
      gggccaggt ggcggaggc cgaagagtg ctacaacac 
      agcgctgcg cttgtgccc ttcacatgc cgactcact 
      caataactt ggccaatat caaacgcga acagggtta 
      catcgagga ggcgactcg actctacct aaaagcact 
      ggaagtgtt cccagagtt tgcagccgc tcacagcaa 
      cctcgcctc cgtgttaca acaacaggg taaactaaa 
      cgaagcgct aatgcatta caaggaggc gatccggat 
      ccagccgac cttcgccga cgcctactc caacatggg 
      gaacaccct gaaggagat gcaggacgt ttcgggcgc 
      cctccagtg ctacactcg ggcgattca gatcaatcc 
      agcttttgc cgacgccca cagcaacct agcgagcat 
      ccacaagga ctcgggaaa cattccaga agcgataca 
      gtcgtaccg aacggcctt gaaactcaa gccggactt 
      tccagacgc ctactgcaa cctcgccca ttgtcttca 
      aatcgtctg tgattggac ggattatga cgccaggat 
      gaagaaatt ggtcagtat tgttgccga ccagctcga 
      gaaaaaccg actaccttc ggtccaccc ccaccactc 
      aatgctcta ccccctatc acacgagtt ccgtaaagc 
      catagcggc acgtcatgc caacttatg cctggaaaa 
      aatccacgt gttgcacaa acaccccta caaattcac 
      cacagagtt aaaggggcg gctgagagt cggttacgt 
      gagcagtga ttttgggaa ccacccaac ctcacactt 
      gatgcagtc ggttccggg gttgcacga ccgctcccg 
      agtcgagat tttctgcta tgcgttgag tcaagacga 
      tgggacgac gttcaggag taaaatatc gcgcgaagc 
      tgagcattt tatcgatct atcgcagat tccctgcaa 
      tgggaaggc ggccgatcg cattcatgc cgatggtat 
      tcatatttt ggtcaatat gaatgggta taccaaagg 
      cgcccgtaa tgagatttt cgcgctgaa gccggcgcc 
      ggttcaggt catgtggtt ggggtatcc cgggaccag 
      tggggcgag tttcatgga ctatttggt gacggatgc 
      ggtgacgtc gccggttga gttggccag tcagtatag 
      cgagaaact ggcctatat gccttatac ttattttgt 
      gggggatca tagacagat gtttccgca tcttaagga 
      gagactcat cgtgagtga taagaataa cagtacaaa 
      ttcgaattt ggccgataa tgtggccat tattaatgc 
      gacggattt gtcgccgtt ggttgaaag tacggaggt 
      taaggaaat caaggaggt cgtcaaggc tgcaaaaat 
      ggtggaaat cagtctaaa agtggccga actccccac 
      aacaacccc cattgaaaa catgatcgc ctctggcca 
      ggtccagac ttcactcaa cggcgtagt ggtccaaaa 
      cggcctcgc caccaccca aaccaacaa caaagccgc 
      caccggtga agaagtccc ccagaacat cgtaatcac 
      gacccgaca acaatacgg actccctga cgacgccgt 
      cgtctattg caatttcaa ccaattgta caaaatcga 
      tccgatgac attgcacat gtgggtcaa catattaaa 
      ggctgtgcc caattccgt tctctggct gttgcgttt 
      cccggccgt tggcgagcc caatttaca agcaacagc 
      acagcaatt ggggcttcc cccaggcag gattctgtt 
      cagtaatgt ggcggctaa agaggagca tgtgaggag 
      gggacagtt ggcggatgt gtgtttgga tacgccctt 
      gtgtaatgg acataccac cagtatgga tgttttgtg 
      gacggggac gccggtcgt gaccttccc gggggaaac 
      cctggcttc aagggtggc tgccagtca gttggctac 
      acttgggtg tccggaatt gatagcacg gtccaggca 
      ggagtatca agaaattgc tatcaagct aggaactga 
      taaggaata tttgaaggc gatgaggca caaagtttg 
      ggcagctcg caccacatc gccgctgtt tgactgcaa 
      acagtacgc ccaaggtct ggaattgtt gtactgcaa 
      aatgtggga acgattcgc tgcagggct caagccaga 
      tcatataac tgacacaac cgcaaaaaa atagaaaca 
      aattgattt ttgtgggac tgatattta ctcatctaa 
      attattttg taatttcta gcttttgga cagagttgt 
      gtgaagact ccagatcat gatgttaat ttagttcaa 
      gttgtatgc agttattat tgagttgtg aaatgtgac 
      ataagatcg ctgtgttgt attataata tatttcaac 
      aagttca
    • >TC003916 RC
      tcattatca ttatttatt aatgtcagc aatttttat 
      tgtctaata tactacaga gcctaccga tcgcacctc 
      atcgggcgt gaaattgat aagacgcct tgtggtttt 
      ttgtgctcc gaagctcgt cacagcccg ggaatagtg 
      caaatggtc ccatctggt aaatcccat tgaatcgca 
      aaaaatcgt tgtgggccc cttcgcacc cattgacga 
      ccaaatcca ggtaaataa cggaccatg gaatggaaa 
      tttccactc gcttccacc aatcacacg ccagctcca 
      gcagctcaa agaccaatc aggtcgcag ctgcgcttt 
      gaccacagt tcttcagtt gtctctctt cagcggcgg 
      ccatttgag gccatcaga gaatcgatt gttgtaatt 
      tagagcgtt ttttggtga aaaactgtg gtgaaagtg 
      tgcagaaat aggtcagat agggtgtat gttggtgta 
      acggcgtgc tttaccatg caggaccag attgtggtc 
      gatggtcag aaccatctc agttctgga acaaacagc 
      gggttgccc atcaccggt agtcccatt gtcttaaag 
      atgaatgat gttccgcag ctttcgtcc gttgggctt 
      cttgaactt gcccatcgt gagtaccaa gctggggat 
      tatgaaaat gcagagagg cattgtatg cagttgtgg 
      agacaggat cagaccaat acgggggtt ttactgctt 
      ttgtcaagc atacatttc cagtgcagg cgtttagac 
      aaatccgca catttcagc actttggca atcaaacaa 
      aatccactc ctcgccgaa gcttacagt aatttgggc 
      aacgtgtac aaagaaaga agtcagctg caagaagca 
      ctcgataat tatcgacat gcagtgcgt cttaaacca 
      gattttatt gatggatac atcaactta gctgcagct 
      cttgttgct gcaggagat atggagcaa gcagttcaa 
      gcatacatt accgcattg cagtacaat ccagatctc 
      tactgtgtt cgtagcgat ttgggtaac ctcctaaaa 
      gcattaggt cgtttagac gaggccaag aatactatt 
      tccatctac tccaattta tttcaatct ccaaaatta 
      aaaaatcaa gtaaaatac aaattttac aaggcataa 
      tataaaaaa cccaggctg atattttcc caagtgcgc 
      attgcgtag agtttcacc ttgtgcgtc ttactaatg 
      tgttaaatt tttacacca agagtgaaa aattagcac 
      atagcattt gcgtaattc catttgaaa cacaccgtt 
      ttatgagta gttttggct actagcgat ttttctggc 
      ctgagatga atgcgttat cagtgcaag cttgcgaag 
      attttttta attatgttt ttatcacac ttgaacagt 
      ttgttactt ttgacgcag tcattttat ttttctcta 
      aattctgag acagttatt agttttgaa ggtaaaaat 
      cgtcaattg tttatttaa aaactaata actgtcgta 
      ttttcaggt gccctgcgt agatttttt ttcagttct 
      tcctatttt ttcttttgt ttactcagt cattgtaac 
      agaaaatat cagcctaaa acaaaatat aaaaaatgg 
      atatgaatt ataaaaagt aaagcagct tatctgtag 
      atactgcaa tcatttcaa ttgaacaac aacctatta 
      ttagtcact tccacgcaa ttattttta attaaagtt 
      cagttttat tttttaaag tcatgaatg ttttatttt 
      taggtaatt attaaattc caatacagt ttaatctac 
      aga