| General information | |||
| TC003916 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 26756382..26788866 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003916 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00638 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)Days post injection
Fertility
mature egg not present -
Number of animals showing the phenotype: 2
egg chamber mostly not present - Number of animals showing the phenotype: 3
egg chamber mostly not present - Number of animals showing the phenotype: 3
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: < 20
embryo/egg not developed/not fertilized -
Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (16 dpi)
Days post injection
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
Days post injection
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
Days post injection
Pupal morphology
Adult morphology
- >TC003916 RA
MQDQIVVDG QNHLSSGTN SGLPITGSP IVLKMNDVP QLSSVGLLE LAHREYQAG DYENAERHC MQLWRQDQT NTGVLLLLS SIHFQCRRL DKSAHFSTL AIKQNPLLA EAYSNLGNV YKERSQLQE ALDNYRHAV RLKPDFIDG YINLAAALV AAGDMEQAV QAYITALQY NPDLYCVRS DLGNLLKAL GRLDEAKAC YLKAIETRP DFAVAWSNL GCVFNAQGE IWLAIHHFE KAVGLDPNF LDAYINLGN VLKEARIFD RAVAAYLRA LNLSPNNAV VHGNLACVY YEQGLIDLA IDTYRRAIE LQPNFPDAY CNLANALKE KGQVAEAEE CYNTALRLC PSHADSLNN LANIKREQG YIEEATRLY LKALEVFPE FAAAHSNLA SVLQQQGKL NEALMHYKE AIRIQPTFA DAYSNMGNT LKEMQDVSG ALQCYTRAI QINPAFADA HSNLASIHK DSGNIPEAI QSYRTALKL KPDFPDAYC NLAHCLQIV CDWTDYDAR MKKLVSIVA DQLEKNRLP SVHPHHSML YPLSHEFRK AIAARHANL CLEKIHVLH KHPYKFTTE LKGRLRVGY VSSDFGNHP TSHLMQSVP GLHDRSRVE IFCYALSQD DGTTFRSKI SREAEHFID LSQIPCNGK AADRIHADG IHILVNMNG YTKGARNEI FALKPAPVQ VMWLGYPGT SGASFMDYL VTDAVTSPV ELASQYSEK LAYMPYTYF VGDHRQMFP HLKERLIVS DKNNSTNSN LADNVAIIN ATDLSPLVE STEVKEIKE VVKAAKMVE ISLKVAELP TTTPIENMI ASGQVQTSL NGVVVQNGL ATTQTNNKA ATGEEVPQN IVITTRQQY GLPDDAVVY CNFNQLYKI DPMTLHMWV NILKAVPNS VLWLLRFPA VGEPNLQAT AQQLGLPPG RILFSNVAA KEEHVRRGQ LADVCLDTP LCNGHTTSM DVLWTGTPV VTFPGETLA SRVAASQLA TLGCPELIA RSRQEYQEI AIKLGTDKE YLKAMRHKV WAARTTSPL FDCKQYAQG LELLYCKMW ERFAAGLKP DHITDTTAK K
- >TC003916 RB
MRSLRFGDF FLSQPACYL KAIETRPDF AVAWSNLGC VFNAQGEIW LAIHHFEKA VGLDPNFLD AYINLGNVL KEARIFDRA VAAYLRALN LSPNNAVVH GNLACVYYE QGLIDLAID TYRRAIELQ PNFPDAYCN LANALKEKG QVAEAEECY NTALRLCPS HADSLNNLA NIKREQGYI EEATRLYLK ALEVFPEFA AAHSNLASV LQQQGKLNE ALMHYKEAI RIQPTFADA YSNMGNTLK EMQDVSGAL QCYTRAIQI NPAFADAHS NLASIHKDS GNIPEAIQS YRTALKLKP DFPDAYCNL AHCLQIVCD WTDYDARMK KLVSIVADQ LEKNRLPSV HPHHSMLYP LSHEFRKAI AARHANLCL EKIHVLHKH PYKFTTELK GRLRVGYVS SDFGNHPTS HLMQSVPGL HDRSRVEIF CYALSQDDG TTFRSKISR EAEHFIDLS QIPCNGKAA DRIHADGIH ILVNMNGYT KGARNEIFA LKPAPVQVM WLGYPGTSG ASFMDYLVT DAVTSPVEL ASQYSEKLA YMPYTYFVG DHRQMFPHL KERLIVSDK NNSTNSNLA DNVAIINAT DLSPLVEST EVKEIKEVV KAAKMVEIS LKVAELPTT TPIENMIAS GQVQTSLNG VVVQNGLAT TQTNNKAAT GEEVPQNIV ITTRQQYGL PDDAVVYCN FNQLYKIDP MTLHMWVNI LKAVPNSVL WLLRFPAVG EPNLQATAQ QLGLPPGRI LFSNVAAKE EHVRRGQLA DVCLDTPLC NGHTTSMDV LWTGTPVVT FPGETLASR VAASQLATL GCPELIARS RQEYQEIAI KLGTDKEYL KAMRHKVWA ARTTSPLFD CKQYAQGLE LLYCKMWER FAAGLKPDH ITDTTAKK
- >TC003916 RC
MQDQIVVDG QNHLSSGTN SGLPITGSP IVLKMNDVP QLSSVGLLE LAHREYQAG DYENAERHC MQLWRQDQT NTGVLLLLS SIHFQCRRL DKSAHFSTL AIKQNPLLA EAYSNLGNV YKERSQLQE ALDNYRHAV RLKPDFIDG YINLAAALV AAGDMEQAV QAYITALQY NPDLYCVRS DLGNLLKAL GRLDEAKNT ISIYSNLFQ SPKLKNQVK YKFYKA
- >TC003916 RA
atgcaggac cagattgtg gtcgatggt cagaaccat ctcagttct ggaacaaac agcgggttg cccatcacc ggtagtccc attgtctta aagatgaat gatgttccg cagctttcg tccgttggg cttcttgaa cttgcccat cgtgagtac caagctggg gattatgaa aatgcagag aggcattgt atgcagttg tggagacag gatcagacc aatacgggg gttttactg cttttgtca agcatacat ttccagtgc aggcgttta gacaaatcc gcacatttc agcactttg gcaatcaaa caaaatcca ctcctcgcc gaagcttac agtaatttg ggcaacgtg tacaaagaa agaagtcag ctgcaagaa gcactcgat aattatcga catgcagtg cgtcttaaa ccagatttt attgatgga tacatcaac ttagctgca gctcttgtt gctgcagga gatatggag caagcagtt caagcatac attaccgca ttgcagtac aatccagat ctctactgt gttcgtagc gatttgggt aacctccta aaagcatta ggtcgttta gacgaggcc aaggcgtgt tatttgaaa gctatcgag actagaccg gatttcgcc gtcgcttgg agcaatctt ggatgcgtt tttaacgct caaggggag atctggctc gcgatacat catttcgaa aaagcagtc ggtttggat cccaatttc ttggatgcc tacatcaat ctgggcaat gtgctgaaa gaagcgcga attttcgat cgggcggta gcggcgtat ttgcgggcc ctaaacctc tcgcccaac aatgccgtc gtccacggc aacctggcc tgtgtctac tacgaacag ggtctcatt gacttggcc atcgacacg tatcgaagg gccatcgag ttgcagccc aacttcccc gacgcctac tgcaacctg gcgaacgcc ctcaaggaa aagggccag gtggcggag gccgaagag tgctacaac acagcgctg cgcttgtgc ccttcacat gccgactca ctcaataac ttggccaat atcaaacgc gaacagggt tacatcgag gaggcgact cgactctac ctaaaagca ctggaagtg ttcccagag tttgcagcc gctcacagc aacctcgcc tccgtgtta caacaacag ggtaaacta aacgaagcg ctaatgcat tacaaggag gcgatccgg atccagccg accttcgcc gacgcctac tccaacatg gggaacacc ctgaaggag atgcaggac gtttcgggc gccctccag tgctacact cgggcgatt cagatcaat ccagctttt gccgacgcc cacagcaac ctagcgagc atccacaag gactcggga aacattcca gaagcgata cagtcgtac cgaacggcc ttgaaactc aagccggac tttccagac gcctactgc aacctcgcc cattgtctt caaatcgtc tgtgattgg acggattat gacgccagg atgaagaaa ttggtcagt attgttgcc gaccagctc gagaaaaac cgactacct tcggtccac ccccaccac tcaatgctc tacccccta tcacacgag ttccgtaaa gccatagcg gcacgtcat gccaactta tgcctggaa aaaatccac gtgttgcac aaacacccc tacaaattc accacagag ttaaagggg cggctgaga gtcggttac gtgagcagt gattttggg aaccaccca acctcacac ttgatgcag tcggttccg gggttgcac gaccgctcc cgagtcgag attttctgc tatgcgttg agtcaagac gatgggacg acgttcagg agtaaaata tcgcgcgaa gctgagcat tttatcgat ctatcgcag attccctgc aatgggaag gcggccgat cgcattcat gccgatggt attcatatt ttggtcaat atgaatggg tataccaaa ggcgcccgt aatgagatt ttcgcgctg aagccggcg ccggttcag gtcatgtgg ttggggtat cccgggacc agtggggcg agtttcatg gactatttg gtgacggat gcggtgacg tcgccggtt gagttggcc agtcagtat agcgagaaa ctggcctat atgccttat acttatttt gtgggggat catagacag atgtttccg catcttaag gagagactc atcgtgagt gataagaat aacagtaca aattcgaat ttggccgat aatgtggcc attattaat gcgacggat ttgtcgccg ttggttgaa agtacggag gttaaggaa atcaaggag gtcgtcaag gctgcaaaa atggtggaa atcagtcta aaagtggcc gaactcccc acaacaacc cccattgaa aacatgatc gcctctggc caggtccag acttcactc aacggcgta gtggtccaa aacggcctc gccaccacc caaaccaac aacaaagcc gccaccggt gaagaagtc ccccagaac atcgtaatc acgacccga caacaatac ggactccct gacgacgcc gtcgtctat tgcaatttc aaccaattg tacaaaatc gatccgatg acattgcac atgtgggtc aacatatta aaggctgtg cccaattcc gttctctgg ctgttgcgt ttcccggcc gttggcgag cccaattta caagcaaca gcacagcaa ttggggctt cccccaggc aggattctg ttcagtaat gtggcggct aaagaggag catgtgagg aggggacag ttggcggat gtgtgtttg gatacgccc ttgtgtaat ggacatacc accagtatg gatgttttg tggacgggg acgccggtc gtgaccttc ccgggggaa accctggct tcaagggtg gctgccagt cagttggct acacttggg tgtccggaa ttgatagca cggtccagg caggagtat caagaaatt gctatcaag ctaggaact gataaggaa tatttgaag gcgatgagg cacaaagtt tgggcagct cgcaccaca tcgccgctg tttgactgc aaacagtac gcccaaggt ctggaattg ttgtactgc aaaatgtgg gaacgattc gctgcaggg ctcaagcca gatcatata actgacaca accgcaaaa aaatag
- >TC003916 RB
atgcgttca ttgcgtttt ggcgatttt tttctgtcc caacctgcg tgttatttg aaagctatc gagactaga ccggatttc gccgtcgct tggagcaat cttggatgc gtttttaac gctcaaggg gagatctgg ctcgcgata catcatttc gaaaaagca gtcggtttg gatcccaat ttcttggat gcctacatc aatctgggc aatgtgctg aaagaagcg cgaattttc gatcgggcg gtagcggcg tatttgcgg gccctaaac ctctcgccc aacaatgcc gtcgtccac ggcaacctg gcctgtgtc tactacgaa cagggtctc attgacttg gccatcgac acgtatcga agggccatc gagttgcag cccaacttc cccgacgcc tactgcaac ctggcgaac gccctcaag gaaaagggc caggtggcg gaggccgaa gagtgctac aacacagcg ctgcgcttg tgcccttca catgccgac tcactcaat aacttggcc aatatcaaa cgcgaacag ggttacatc gaggaggcg actcgactc tacctaaaa gcactggaa gtgttccca gagtttgca gccgctcac agcaacctc gcctccgtg ttacaacaa cagggtaaa ctaaacgaa gcgctaatg cattacaag gaggcgatc cggatccag ccgaccttc gccgacgcc tactccaac atggggaac accctgaag gagatgcag gacgtttcg ggcgccctc cagtgctac actcgggcg attcagatc aatccagct tttgccgac gcccacagc aacctagcg agcatccac aaggactcg ggaaacatt ccagaagcg atacagtcg taccgaacg gccttgaaa ctcaagccg gactttcca gacgcctac tgcaacctc gcccattgt cttcaaatc gtctgtgat tggacggat tatgacgcc aggatgaag aaattggtc agtattgtt gccgaccag ctcgagaaa aaccgacta ccttcggtc cacccccac cactcaatg ctctacccc ctatcacac gagttccgt aaagccata gcggcacgt catgccaac ttatgcctg gaaaaaatc cacgtgttg cacaaacac ccctacaaa ttcaccaca gagttaaag gggcggctg agagtcggt tacgtgagc agtgatttt gggaaccac ccaacctca cacttgatg cagtcggtt ccggggttg cacgaccgc tcccgagtc gagattttc tgctatgcg ttgagtcaa gacgatggg acgacgttc aggagtaaa atatcgcgc gaagctgag cattttatc gatctatcg cagattccc tgcaatggg aaggcggcc gatcgcatt catgccgat ggtattcat attttggtc aatatgaat gggtatacc aaaggcgcc cgtaatgag attttcgcg ctgaagccg gcgccggtt caggtcatg tggttgggg tatcccggg accagtggg gcgagtttc atggactat ttggtgacg gatgcggtg acgtcgccg gttgagttg gccagtcag tatagcgag aaactggcc tatatgcct tatacttat tttgtgggg gatcataga cagatgttt ccgcatctt aaggagaga ctcatcgtg agtgataag aataacagt acaaattcg aatttggcc gataatgtg gccattatt aatgcgacg gatttgtcg ccgttggtt gaaagtacg gaggttaag gaaatcaag gaggtcgtc aaggctgca aaaatggtg gaaatcagt ctaaaagtg gccgaactc cccacaaca acccccatt gaaaacatg atcgcctct ggccaggtc cagacttca ctcaacggc gtagtggtc caaaacggc ctcgccacc acccaaacc aacaacaaa gccgccacc ggtgaagaa gtcccccag aacatcgta atcacgacc cgacaacaa tacggactc cctgacgac gccgtcgtc tattgcaat ttcaaccaa ttgtacaaa atcgatccg atgacattg cacatgtgg gtcaacata ttaaaggct gtgcccaat tccgttctc tggctgttg cgtttcccg gccgttggc gagcccaat ttacaagca acagcacag caattgggg cttccccca ggcaggatt ctgttcagt aatgtggcg gctaaagag gagcatgtg aggagggga cagttggcg gatgtgtgt ttggatacg cccttgtgt aatggacat accaccagt atggatgtt ttgtggacg gggacgccg gtcgtgacc ttcccgggg gaaaccctg gcttcaagg gtggctgcc agtcagttg gctacactt gggtgtccg gaattgata gcacggtcc aggcaggag tatcaagaa attgctatc aagctagga actgataag gaatatttg aaggcgatg aggcacaaa gtttgggca gctcgcacc acatcgccg ctgtttgac tgcaaacag tacgcccaa ggtctggaa ttgttgtac tgcaaaatg tgggaacga ttcgctgca gggctcaag ccagatcat ataactgac acaaccgca aaaaaatag
- >TC003916 RC
atgcaggac cagattgtg gtcgatggt cagaaccat ctcagttct ggaacaaac agcgggttg cccatcacc ggtagtccc attgtctta aagatgaat gatgttccg cagctttcg tccgttggg cttcttgaa cttgcccat cgtgagtac caagctggg gattatgaa aatgcagag aggcattgt atgcagttg tggagacag gatcagacc aatacgggg gttttactg cttttgtca agcatacat ttccagtgc aggcgttta gacaaatcc gcacatttc agcactttg gcaatcaaa caaaatcca ctcctcgcc gaagcttac agtaatttg ggcaacgtg tacaaagaa agaagtcag ctgcaagaa gcactcgat aattatcga catgcagtg cgtcttaaa ccagatttt attgatgga tacatcaac ttagctgca gctcttgtt gctgcagga gatatggag caagcagtt caagcatac attaccgca ttgcagtac aatccagat ctctactgt gttcgtagc gatttgggt aacctccta aaagcatta ggtcgttta gacgaggcc aagaatact atttccatc tactccaat ttatttcaa tctccaaaa ttaaaaaat caagtaaaa tacaaattt tacaaggca taa
- >TC003916 RA
tcattatca ttatttatt aatgtcagc aatttttat tgtctaata tactacaga gcctaccga tcgcacctc atcgggcgt gaaattgat aagacgcct tgtggtttt ttgtgctcc gaagctcgt cacagcccg ggaatagtg caaatggtc ccatctggt aaatcccat tgaatcgca aaaaatcgt tgtgggccc cttcgcacc cattgacga ccaaatcca ggtaaataa cggaccatg gaatggaaa tttccactc gcttccacc aatcacacg ccagctcca gcagctcaa agaccaatc aggtcgcag ctgcgcttt gaccacagt tcttcagtt gtctctctt cagcggcgg ccatttgag gccatcaga gaatcgatt gttgtaatt tagagcgtt ttttggtga aaaactgtg gtgaaagtg tgcagaaat aggtcagat agggtgtat gttggtgta acggcgtgc tttaccatg caggaccag attgtggtc gatggtcag aaccatctc agttctgga acaaacagc gggttgccc atcaccggt agtcccatt gtcttaaag atgaatgat gttccgcag ctttcgtcc gttgggctt cttgaactt gcccatcgt gagtaccaa gctggggat tatgaaaat gcagagagg cattgtatg cagttgtgg agacaggat cagaccaat acgggggtt ttactgctt ttgtcaagc atacatttc cagtgcagg cgtttagac aaatccgca catttcagc actttggca atcaaacaa aatccactc ctcgccgaa gcttacagt aatttgggc aacgtgtac aaagaaaga agtcagctg caagaagca ctcgataat tatcgacat gcagtgcgt cttaaacca gattttatt gatggatac atcaactta gctgcagct cttgttgct gcaggagat atggagcaa gcagttcaa gcatacatt accgcattg cagtacaat ccagatctc tactgtgtt cgtagcgat ttgggtaac ctcctaaaa gcattaggt cgtttagac gaggccaag gcgtgttat ttgaaagct atcgagact agaccggat ttcgccgtc gcttggagc aatcttgga tgcgttttt aacgctcaa ggggagatc tggctcgcg atacatcat ttcgaaaaa gcagtcggt ttggatccc aatttcttg gatgcctac atcaatctg ggcaatgtg ctgaaagaa gcgcgaatt ttcgatcgg gcggtagcg gcgtatttg cgggcccta aacctctcg cccaacaat gccgtcgtc cacggcaac ctggcctgt gtctactac gaacagggt ctcattgac ttggccatc gacacgtat cgaagggcc atcgagttg cagcccaac ttccccgac gcctactgc aacctggcg aacgccctc aaggaaaag ggccaggtg gcggaggcc gaagagtgc tacaacaca gcgctgcgc ttgtgccct tcacatgcc gactcactc aataacttg gccaatatc aaacgcgaa cagggttac atcgaggag gcgactcga ctctaccta aaagcactg gaagtgttc ccagagttt gcagccgct cacagcaac ctcgcctcc gtgttacaa caacagggt aaactaaac gaagcgcta atgcattac aaggaggcg atccggatc cagccgacc ttcgccgac gcctactcc aacatgggg aacaccctg aaggagatg caggacgtt tcgggcgcc ctccagtgc tacactcgg gcgattcag atcaatcca gcttttgcc gacgcccac agcaaccta gcgagcatc cacaaggac tcgggaaac attccagaa gcgatacag tcgtaccga acggccttg aaactcaag ccggacttt ccagacgcc tactgcaac ctcgcccat tgtcttcaa atcgtctgt gattggacg gattatgac gccaggatg aagaaattg gtcagtatt gttgccgac cagctcgag aaaaaccga ctaccttcg gtccacccc caccactca atgctctac cccctatca cacgagttc cgtaaagcc atagcggca cgtcatgcc aacttatgc ctggaaaaa atccacgtg ttgcacaaa cacccctac aaattcacc acagagtta aaggggcgg ctgagagtc ggttacgtg agcagtgat tttgggaac cacccaacc tcacacttg atgcagtcg gttccgggg ttgcacgac cgctcccga gtcgagatt ttctgctat gcgttgagt caagacgat gggacgacg ttcaggagt aaaatatcg cgcgaagct gagcatttt atcgatcta tcgcagatt ccctgcaat gggaaggcg gccgatcgc attcatgcc gatggtatt catattttg gtcaatatg aatgggtat accaaaggc gcccgtaat gagattttc gcgctgaag ccggcgccg gttcaggtc atgtggttg gggtatccc gggaccagt ggggcgagt ttcatggac tatttggtg acggatgcg gtgacgtcg ccggttgag ttggccagt cagtatagc gagaaactg gcctatatg ccttatact tattttgtg ggggatcat agacagatg tttccgcat cttaaggag agactcatc gtgagtgat aagaataac agtacaaat tcgaatttg gccgataat gtggccatt attaatgcg acggatttg tcgccgttg gttgaaagt acggaggtt aaggaaatc aaggaggtc gtcaaggct gcaaaaatg gtggaaatc agtctaaaa gtggccgaa ctccccaca acaaccccc attgaaaac atgatcgcc tctggccag gtccagact tcactcaac ggcgtagtg gtccaaaac ggcctcgcc accacccaa accaacaac aaagccgcc accggtgaa gaagtcccc cagaacatc gtaatcacg acccgacaa caatacgga ctccctgac gacgccgtc gtctattgc aatttcaac caattgtac aaaatcgat ccgatgaca ttgcacatg tgggtcaac atattaaag gctgtgccc aattccgtt ctctggctg ttgcgtttc ccggccgtt ggcgagccc aatttacaa gcaacagca cagcaattg gggcttccc ccaggcagg attctgttc agtaatgtg gcggctaaa gaggagcat gtgaggagg ggacagttg gcggatgtg tgtttggat acgcccttg tgtaatgga cataccacc agtatggat gttttgtgg acggggacg ccggtcgtg accttcccg ggggaaacc ctggcttca agggtggct gccagtcag ttggctaca cttgggtgt ccggaattg atagcacgg tccaggcag gagtatcaa gaaattgct atcaagcta ggaactgat aaggaatat ttgaaggcg atgaggcac aaagtttgg gcagctcgc accacatcg ccgctgttt gactgcaaa cagtacgcc caaggtctg gaattgttg tactgcaaa atgtgggaa cgattcgct gcagggctc aagccagat catataact gacacaacc gcaaaaaaa tagaaacaa attgatttt tgtgggact gatatttac tcatctaaa ttattttgt aatttctag cttttggac agagttgtg tgaagactc cagatcatg atgttaatt tagttcaag ttgtatgca gttattatt gagttgtga aatgtgaca taagatcgc tgtgttgta ttataatat atttcaaca agttca
- >TC003916 RB
tttgagcta aaatctctg gtcttttgc tagattttg ttaatatgt gatgggaaa tcgacgaaa atggcgatt tttgggcgt tttacaaag tatttcact cagaagtgt aattaattc gagaaattt tttaaaaat aaatcgaaa gaacactaa atttggtct aattttaac cttgttcga gcgtttaag cacattttt acgctgaaa acccgggag gtaattgtt taacgtata ttataaaaa atataattg aaaaatggt tgcattggg actattttt taaaatgct ttaccgctg aataccttt ttaagaaaa aacatttac gtgaaattt taaacacag aattgagtt taaacacag agaagctca ataattccg gcaatttca ttaaaaatg caagttttg tgaaatgca attattttg agtaaaaat aaatcgaaa agttaaaaa ttgattaga catttgtgt tattaaacg atgttgcgt tgtattttg taataattt ttagcaaaa aattcagtt cttggcgaa aattttcga aacactgaa ttgggttgc aggtgatat tatttatcg ttgatgtgt tttattttg tacattttt atgcagaaa ctaaataaa aaaatcgaa tttcgtcga aaatgcggc tgtttttgt cattccttt gcactttaa aaagtttct tgagtttaa attaattat agttttttt tgagcattt taatacatt ttttagcca attatttta cggagcatt gtcaaaaat aagctgaaa actcacaaa aagttgttt tggtacgtt tttatgcat aaactaatt aatttcgcg agattttgt aaaaaatgg tgcagtttt tgtcattta ttgcacttc ttactagta gttttaaaa tgaattgca ttagcttca tttgacatc aggttaaaa agtgccgaa gtttgtctt aatgttact tctttatta cttttttta ttataattt ttctttctc tgggtactg cgaaattat catcaataa aattttctg tttataaaa cgtcaaaag aatctggaa ataaatcag ttgtttcgc gaataaagt tatcatgag ctgttttgc gtgaattta atttcaagc tacatttgc tgtaatagc tttttctag aggaatgct tgtaaatta agctaaatt tttgcattt aaatagggc gttagggag catttatta aaaaaagtt taatattaa aataacaac tttcggagg tttttgatt tttgtttat taataacga agatattta ccgggaacg tctcagttg agacactgt tttttaatt ttgttgctt ttgttattt taattatcg caaaaaaga gtttcacca aaatatcgg tatcaatgt ttttttttt cctaaagaa gcttattta catcaacta gacagtact ttaaggaaa attcgaact ctgaaattg gaagcataa ataaataat cagttagtt caatttttt ttgctcatt tagctgaga atttttacc agttttctt cgcactgtt gtatcttat gccttggga agatgaagc ggtaggcgt ataaaaata aatatttca ggagagttg atatttcct ttgggtacg cggagtgtc tttggattc aggtgcgat caaatggga acaaaatct gcttttgtg tcagttttg ttccaaatg gttgtgagc cagctttca gggacaccc gtttcagtg aaaatgacg gtgaacatt gtaaataag ttgtttctt ttaagttta ggcccggtg gcattgagt ttctggttt agagagtgg gaatagctt tggtgattt tctgtttgt ggccgccta taattgaca aatgagaat gctgactaa tggtattac tgttgctgt acaataata caggtatgt ggctggtgt cccgtgcgc ctccctgga atcacactg gtaaaacta tgggcttag tctcaggta atgtacgca agcgaacaa gatgtttct ttgttttta ggtattgga gtgaggcct ctctgtgcg tcaattgct tgcttgcgt aatgagttt tcttctttt tttcgaccg tttctatgt cgattttaa ggtgcgtaa gttgtgcgc tggcgtatt ttttcatct cagtatgcg ttcattgcg ttttggcga tttttttct gtcccaacc tgcgtgtta tttgaaagc tatcgagac tagaccgga tttcgccgt cgcttggag caatcttgg atgcgtttt taacgctca aggggagat ctggctcgc gatacatca tttcgaaaa agcagtcgg tttggatcc caatttctt ggatgccta catcaatct gggcaatgt gctgaaaga agcgcgaat tttcgatcg ggcggtagc ggcgtattt gcgggccct aaacctctc gcccaacaa tgccgtcgt ccacggcaa cctggcctg tgtctacta cgaacaggg tctcattga cttggccat cgacacgta tcgaagggc catcgagtt gcagcccaa cttccccga cgcctactg caacctggc gaacgccct caaggaaaa gggccaggt ggcggaggc cgaagagtg ctacaacac agcgctgcg cttgtgccc ttcacatgc cgactcact caataactt ggccaatat caaacgcga acagggtta catcgagga ggcgactcg actctacct aaaagcact ggaagtgtt cccagagtt tgcagccgc tcacagcaa cctcgcctc cgtgttaca acaacaggg taaactaaa cgaagcgct aatgcatta caaggaggc gatccggat ccagccgac cttcgccga cgcctactc caacatggg gaacaccct gaaggagat gcaggacgt ttcgggcgc cctccagtg ctacactcg ggcgattca gatcaatcc agcttttgc cgacgccca cagcaacct agcgagcat ccacaagga ctcgggaaa cattccaga agcgataca gtcgtaccg aacggcctt gaaactcaa gccggactt tccagacgc ctactgcaa cctcgccca ttgtcttca aatcgtctg tgattggac ggattatga cgccaggat gaagaaatt ggtcagtat tgttgccga ccagctcga gaaaaaccg actaccttc ggtccaccc ccaccactc aatgctcta ccccctatc acacgagtt ccgtaaagc catagcggc acgtcatgc caacttatg cctggaaaa aatccacgt gttgcacaa acaccccta caaattcac cacagagtt aaaggggcg gctgagagt cggttacgt gagcagtga ttttgggaa ccacccaac ctcacactt gatgcagtc ggttccggg gttgcacga ccgctcccg agtcgagat tttctgcta tgcgttgag tcaagacga tgggacgac gttcaggag taaaatatc gcgcgaagc tgagcattt tatcgatct atcgcagat tccctgcaa tgggaaggc ggccgatcg cattcatgc cgatggtat tcatatttt ggtcaatat gaatgggta taccaaagg cgcccgtaa tgagatttt cgcgctgaa gccggcgcc ggttcaggt catgtggtt ggggtatcc cgggaccag tggggcgag tttcatgga ctatttggt gacggatgc ggtgacgtc gccggttga gttggccag tcagtatag cgagaaact ggcctatat gccttatac ttattttgt gggggatca tagacagat gtttccgca tcttaagga gagactcat cgtgagtga taagaataa cagtacaaa ttcgaattt ggccgataa tgtggccat tattaatgc gacggattt gtcgccgtt ggttgaaag tacggaggt taaggaaat caaggaggt cgtcaaggc tgcaaaaat ggtggaaat cagtctaaa agtggccga actccccac aacaacccc cattgaaaa catgatcgc ctctggcca ggtccagac ttcactcaa cggcgtagt ggtccaaaa cggcctcgc caccaccca aaccaacaa caaagccgc caccggtga agaagtccc ccagaacat cgtaatcac gacccgaca acaatacgg actccctga cgacgccgt cgtctattg caatttcaa ccaattgta caaaatcga tccgatgac attgcacat gtgggtcaa catattaaa ggctgtgcc caattccgt tctctggct gttgcgttt cccggccgt tggcgagcc caatttaca agcaacagc acagcaatt ggggcttcc cccaggcag gattctgtt cagtaatgt ggcggctaa agaggagca tgtgaggag gggacagtt ggcggatgt gtgtttgga tacgccctt gtgtaatgg acataccac cagtatgga tgttttgtg gacggggac gccggtcgt gaccttccc gggggaaac cctggcttc aagggtggc tgccagtca gttggctac acttgggtg tccggaatt gatagcacg gtccaggca ggagtatca agaaattgc tatcaagct aggaactga taaggaata tttgaaggc gatgaggca caaagtttg ggcagctcg caccacatc gccgctgtt tgactgcaa acagtacgc ccaaggtct ggaattgtt gtactgcaa aatgtggga acgattcgc tgcagggct caagccaga tcatataac tgacacaac cgcaaaaaa atagaaaca aattgattt ttgtgggac tgatattta ctcatctaa attattttg taatttcta gcttttgga cagagttgt gtgaagact ccagatcat gatgttaat ttagttcaa gttgtatgc agttattat tgagttgtg aaatgtgac ataagatcg ctgtgttgt attataata tatttcaac aagttca
- >TC003916 RC
tcattatca ttatttatt aatgtcagc aatttttat tgtctaata tactacaga gcctaccga tcgcacctc atcgggcgt gaaattgat aagacgcct tgtggtttt ttgtgctcc gaagctcgt cacagcccg ggaatagtg caaatggtc ccatctggt aaatcccat tgaatcgca aaaaatcgt tgtgggccc cttcgcacc cattgacga ccaaatcca ggtaaataa cggaccatg gaatggaaa tttccactc gcttccacc aatcacacg ccagctcca gcagctcaa agaccaatc aggtcgcag ctgcgcttt gaccacagt tcttcagtt gtctctctt cagcggcgg ccatttgag gccatcaga gaatcgatt gttgtaatt tagagcgtt ttttggtga aaaactgtg gtgaaagtg tgcagaaat aggtcagat agggtgtat gttggtgta acggcgtgc tttaccatg caggaccag attgtggtc gatggtcag aaccatctc agttctgga acaaacagc gggttgccc atcaccggt agtcccatt gtcttaaag atgaatgat gttccgcag ctttcgtcc gttgggctt cttgaactt gcccatcgt gagtaccaa gctggggat tatgaaaat gcagagagg cattgtatg cagttgtgg agacaggat cagaccaat acgggggtt ttactgctt ttgtcaagc atacatttc cagtgcagg cgtttagac aaatccgca catttcagc actttggca atcaaacaa aatccactc ctcgccgaa gcttacagt aatttgggc aacgtgtac aaagaaaga agtcagctg caagaagca ctcgataat tatcgacat gcagtgcgt cttaaacca gattttatt gatggatac atcaactta gctgcagct cttgttgct gcaggagat atggagcaa gcagttcaa gcatacatt accgcattg cagtacaat ccagatctc tactgtgtt cgtagcgat ttgggtaac ctcctaaaa gcattaggt cgtttagac gaggccaag aatactatt tccatctac tccaattta tttcaatct ccaaaatta aaaaatcaa gtaaaatac aaattttac aaggcataa tataaaaaa cccaggctg atattttcc caagtgcgc attgcgtag agtttcacc ttgtgcgtc ttactaatg tgttaaatt tttacacca agagtgaaa aattagcac atagcattt gcgtaattc catttgaaa cacaccgtt ttatgagta gttttggct actagcgat ttttctggc ctgagatga atgcgttat cagtgcaag cttgcgaag attttttta attatgttt ttatcacac ttgaacagt ttgttactt ttgacgcag tcattttat ttttctcta aattctgag acagttatt agttttgaa ggtaaaaat cgtcaattg tttatttaa aaactaata actgtcgta ttttcaggt gccctgcgt agatttttt ttcagttct tcctatttt ttcttttgt ttactcagt cattgtaac agaaaatat cagcctaaa acaaaatat aaaaaatgg atatgaatt ataaaaagt aaagcagct tatctgtag atactgcaa tcatttcaa ttgaacaac aacctatta ttagtcact tccacgcaa ttattttta attaaagtt cagttttat tttttaaag tcatgaatg ttttatttt taggtaatt attaaattc caatacagt ttaatctac aga
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