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Gene information
General information
TC003905 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26495636..26504306
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003905
    iBeetle screen iB_00634

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt precocious - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003905 RA
      MLKIFLLAI VTTALAAPQ PPLQAAEAC DASKCKLPE 
      CRCASTNPP EGLDLEQIP QFVFLTFDD AVQITNYEI 
      YTELFYNKT NPDGCPVQA TFFLSHEYT DYTKVHELY 
      VNKQEIALH SITHQALTD YWRNLTLDG LQAEFGDEA 
      TLITHFANI PQEAFKGMR IPFLQLSGD NSFQFAKQL 
      GLTYDCSWP TQTFRKPGL WPYTLNYKS NQDCPIGPC 
      PQSSIPGVW VVPMIDWTD LSNNVCSMV DACVDIPDD 
      DADKLLQWF IDNFNVQYK GNKAPFGFY IHAAYFAVN 
      PVRLEAYKK FVDYLQGLN DVYLVSPSK AIEWIKNPV 
      PMGAEGWPA CPDVEDLGC TSQTCQLMK EDDPNPRYM 
      TFCGSECPA VYPWLGNPL GEK
    CDS sequence
    • >TC003905 RA
      atgttgaaa atatttctt ttggcgata gtcactacg 
      gccctcgcg gccccccag ccccctttg caagccgct 
      gaagcttgc gacgcttcc aagtgcaag ctgcctgaa 
      tgcaggtgt gcctccaca aacccccct gaagggctc 
      gacctcgag caaatcccc cagttcgtt ttccttaca 
      ttcgacgat gccgtccaa atcacaaat tacgagatc 
      tacaccgaa ctgttctac aacaagacc aatcctgac 
      ggttgtccc gtccaagca acattcttc ttgtcccac 
      gagtacacg gattacacc aaagtgcac gaattgtac 
      gtaaacaaa caggaaatc gctctgcat tcgatcacg 
      catcaggct ttgaccgat tattggagg aatctcact 
      ctggacgga cttcaagca gagttcggg gatgaagcg 
      actttgatt acacacttt gccaatatc ccacaggag 
      gccttcaag ggaatgagg atccccttt ttgcagttg 
      tcaggagat aacagcttc caatttgcc aaacagctc 
      ggtctgacc tacgactgc tcgtggccg acccagacc 
      ttccggaag cccggactt tggccctac acccttaac 
      tacaagtcg aaccaggac tgtcccatc ggcccatgc 
      ccgcaaagc tcgattccg ggcgtgtgg gtggtgccc 
      atgatcgac tggactgac ttgagcaac aacgtgtgc 
      tccatggtc gatgcttgt gtcgacatt cccgacgat 
      gacgccgac aaacttctc cagtggttt atcgacaat 
      ttcaacgtc caatacaaa ggcaataaa gcaccattt 
      gggttctac atccatgct gcttacttt gccgtaaat 
      ccggtgcgt cttgaggcg tataaaaaa ttcgtcgat 
      tatttgcaa ggtctgaat gatgtgtat cttgtgagt 
      ccgagtaaa gccatcgag tggatcaag aaccctgta 
      cctatgggg gccgaaggc tggcctgcg tgtcctgat 
      gtagaggat ttgggttgc acgtctcaa acttgccag 
      ttgatgaag gaggatgat ccgaatccg aggtatatg 
      acattctgt gggtccgaa tgcccagct gtgtatccc 
      tggcttggg aatccgttg ggggagaag taa
    mRNA sequence
    • >TC003905 RA
      gtgtttttc tctgaagta tttgcaata tgttgaaaa 
      tatttcttt tggcgatag tcactacgg ccctcgcgg 
      ccccccagc cccctttgc aagccgctg aagcttgcg 
      acgcttcca agtgcaagc tgcctgaat gcaggtgtg 
      cctccacaa acccccctg aagggctcg acctcgagc 
      aaatccccc agttcgttt tccttacat tcgacgatg 
      ccgtccaaa tcacaaatt acgagatct acaccgaac 
      tgttctaca acaagacca atcctgacg gttgtcccg 
      tccaagcaa cattcttct tgtcccacg agtacacgg 
      attacacca aagtgcacg aattgtacg taaacaaac 
      aggaaatcg ctctgcatt cgatcacgc atcaggctt 
      tgaccgatt attggagga atctcactc tggacggac 
      ttcaagcag agttcgggg atgaagcga ctttgatta 
      cacactttg ccaatatcc cacaggagg ccttcaagg 
      gaatgagga tcccctttt tgcagttgt caggagata 
      acagcttcc aatttgcca aacagctcg gtctgacct 
      acgactgct cgtggccga cccagacct tccggaagc 
      ccggacttt ggccctaca cccttaact acaagtcga 
      accaggact gtcccatcg gcccatgcc cgcaaagct 
      cgattccgg gcgtgtggg tggtgccca tgatcgact 
      ggactgact tgagcaaca acgtgtgct ccatggtcg 
      atgcttgtg tcgacattc ccgacgatg acgccgaca 
      aacttctcc agtggttta tcgacaatt tcaacgtcc 
      aatacaaag gcaataaag caccatttg ggttctaca 
      tccatgctg cttactttg ccgtaaatc cggtgcgtc 
      ttgaggcgt ataaaaaat tcgtcgatt atttgcaag 
      gtctgaatg atgtgtatc ttgtgagtc cgagtaaag 
      ccatcgagt ggatcaaga accctgtac ctatggggg 
      ccgaaggct ggcctgcgt gtcctgatg tagaggatt 
      tgggttgca cgtctcaaa cttgccagt tgatgaagg 
      aggatgatc cgaatccga ggtatatga cattctgtg 
      ggtccgaat gcccagctg tgtatccct ggcttggga 
      atccgttgg gggagaagt aaatatttt tcgtagatt 
      tgtcaaaat caattgaat aaacaaata atgttactg 
      atttttgta aaaatattt tcgtaaaaa aaaacatcc 
      aagtgttta tttattagc tttgtttag tgtgattct 
      atcggttcc acaacaaat aggtttctc caaagaata 
      aaaggaaaa gaaaagaac tgttacacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY