Status
Please wait ...
Gene information
General information
TC003902 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 1040609..1045293
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003902
    iBeetle screen iB_00633
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    elytra partially blistered - Number of animals showing the phenotype: 4

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003902 RA
      MSVPNDQEN YSKAVEKNP KSGNRAALS NLENILPQN 
      AFNIRIKKT IKTTATETK IRSASASEA TTQVKFAYK 
      KENITERKN GEFNIDEVF VEGEYHYLT YLKPEENRP 
      KTKSEDLGE KIDRTLPMV RVTAPTCER KLEVKKPAK 
      KQTLRKFSN RRKQANLEE LFRNCLYEE DYFDVNFRH 
      MLLEEGNYV YRKQFLKKS IDENYRMLV VNWLVYAHQ 
      VLELSENTF FVAVRMFDY VVASQKTAG NKYILTAIT 
      STWIANKMF DVRQIHISK LFALCNGKF SNQQFLSTE 
      REILKVFKF DINISEPSS FLFYFVKLE NLELDSKIC 
      YSALFLLEI FALLLDFSS LPTSFLAAV ALYLVLVKY 
      ELDTKLHLE VAETYCLDK ESFVRKSEQ LALCVEHLL 
      KEKNASSEI VAKYSTEMR GQVARNFVS
    CDS sequence
    • >TC003902 RA
      atgtctgtt ccaaatgac caagaaaat tactcaaaa 
      gccgtcgaa aaaaatccc aaatcggga aaccgtgcc 
      gccctctcc aacctcgaa aacatcctc cctcagaac 
      gcatttaac atccgcatt aagaaaaca attaagaca 
      actgcaacc gaaacgaaa attcgctca gcttcggcc 
      agtgaagcc accacccag gtgaagttc gcctacaag 
      aaagaaaac atcaccgag agaaaaaac ggggagttt 
      aacatcgac gaagtcttc gtcgagggg gagtaccac 
      tacctcact tacctcaag cctgaggaa aataggcca 
      aagacaaag tctgaagat ttgggcgag aaaattgat 
      cgaacttta ccgatggtg agagttacg gcaccgact 
      tgtgagagg aagctcgag gtgaagaaa ccagccaag 
      aagcagacg ctcaggaaa ttcagcaac aggcgcaaa 
      caggccaat ttggaagaa ctgtttcgg aactgtttg 
      tacgaggag gactatttt gatgtgaac ttccggcat 
      atgctttta gaagagggc aactacgtc taccgcaaa 
      caatttttg aaaaaatcc atcgacgag aattacaga 
      atgctcgtc gtcaattgg ctggtctac gcccatcaa 
      gtgttggaa ttgtccgaa aacacgttc ttcgttgct 
      gtccgaatg ttcgattat gttgtagca tctcagaaa 
      acggctgga aataaatac attttaacc gctatcacg 
      agcacttgg atcgctaac aaaatgttc gatgttcgt 
      caaatacat atctccaaa ctgttcgcc ttgtgcaac 
      ggcaagttt tccaatcag cagtttcta tcaacggag 
      cgggaaatc ttgaaagtg ttcaaattc gatattaac 
      atctctgaa cccagttcg tttctgttt tatttcgtc 
      aagttggag aatttagag ctggactca aaaatttgc 
      tacagtgcg ttgtttctc ctcgaaatt tttgcttta 
      cttttggac ttttcctct ctgccgacg tcgtttctc 
      gctgctgtg gccttgtat ctagttttg gtaaaatac 
      gagcttgac accaaattg catctggaa gtggcggaa 
      acatactgt cttgacaaa gaatcgttt gtacggaag 
      tccgagcaa cttgcactt tgtgttgag catttgttg 
      aaggaaaag aatgcgagc agcgaaatt gtggccaaa 
      tattcgacc gaaatgagg ggtcaagtc gctcgtaat 
      tttgtgtct taa
    mRNA sequence
    • >TC003902 RA
      ttcttcgac taaagatac gatctagaa agaacattt 
      ttgccagtg gaccacaag cagtgtaac aaagtgcaa 
      aatgtctgt tccaaatga ccaagaaaa ttactcaaa 
      agccgtcga aaaaaatcc caaatcggg aaaccgtgc 
      cgccctctc caacctcga aaacatcct ccctcagaa 
      cgcatttaa catccgcat taagaaaac aattaagac 
      aactgcaac cgaaacgaa aattcgctc agcttcggc 
      cagtgaagc caccaccca ggtgaagtt cgcctacaa 
      gaaagaaaa catcaccga gagaaaaaa cggggagtt 
      taacatcga cgaagtctt cgtcgaggg ggagtacca 
      ctacctcac ttacctcaa gcctgagga aaataggcc 
      aaagacaaa gtctgaaga tttgggcga gaaaattga 
      tcgaacttt accgatggt gagagttac ggcaccgac 
      ttgtgagag gaagctcga ggtgaagaa accagccaa 
      gaagcagac gctcaggaa attcagcaa caggcgcaa 
      acaggccaa tttggaaga actgtttcg gaactgttt 
      gtacgagga ggactattt tgatgtgaa cttccggca 
      tatgctttt agaagaggg caactacgt ctaccgcaa 
      acaattttt gaaaaaatc catcgacga gaattacag 
      aatgctcgt cgtcaattg gctggtcta cgcccatca 
      agtgttgga attgtccga aaacacgtt cttcgttgc 
      tgtccgaat gttcgatta tgttgtagc atctcagaa 
      aacggctgg aaataaata cattttaac cgctatcac 
      gagcacttg gatcgctaa caaaatgtt cgatgttcg 
      tcaaataca tatctccaa actgttcgc cttgtgcaa 
      cggcaagtt ttccaatca gcagtttct atcaacgga 
      gcgggaaat cttgaaagt gttcaaatt cgatattaa 
      catctctga acccagttc gtttctgtt ttatttcgt 
      caagttgga gaatttaga gctggactc aaaaatttg 
      ctacagtgc gttgtttct cctcgaaat ttttgcttt 
      acttttgga cttttcctc tctgccgac gtcgtttct 
      cgctgctgt ggccttgta tctagtttt ggtaaaata 
      cgagcttga caccaaatt gcatctgga agtggcgga 
      aacatactg tcttgacaa agaatcgtt tgtacggaa 
      gtccgagca acttgcact ttgtgttga gcatttgtt 
      gaaggaaaa gaatgcgag cagcgaaat tgtggccaa 
      atattcgac cgaaatgag gggtcaagt cgctcgtaa 
      ttttgtgtc ttaataatt gtttgtttt aattttaga 
      ccgtgctta attattgtg atgttttac ccaatgaat 
      tttacataa aatgctgga tcagttcat acttgcctt 
      a