Status
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Gene information
General information
TC003895 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26249940..26256327
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003895
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00632

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003895 RA
      MAGLTTLLP DVAKIVPKV SFSWYDYIL FCVMLGLSA 
      LIGVYFGCF GSKQRSADE YLMGNKKMK VFPISVSLV 
      ASHVSGITL LAVPADIYR YGASYWLLI IAAIFVTFT 
      TIYVYLPVF YKLQITSTY EYLERRFDN RNRMFASFL 
      FALGLFLYL PIVIYIPAL ALSAATGIN IHLITPVVC 
      GVCIFYTTL GGLKAVVWT DTLQFTITT GAVIAIFVI 
      GVRAGGGFG AIWSKALEG HRLDIFDFD PDPTKRDSF 
      WITSIGMSI GWLSMVGIH QSCVQKFLS VSTFQESKQ 
      CVIYFSIGI SIVSSFSVF TGLMMYARY ANCDPFTTG 
      TVQKNDQLL PYYVMDVAS YVPGLSGLF IAGIFCAAL 
      STLSACLNC LAGTMYEDF ISKIIGHVE EKTTTYILK 
      LLVVITGVV CTVLVFVIE HLGGILALA ISLAGVTSG 
      PLLGMFTLG VLFPKASSK GSLYGAIIA LLFASWIVT 
      MAQYYKAQG SIVDVPKPT SVDGCLANF TTLSPLTTL 
      NSTLSSLES VATEDITIE NSTISQLLS NFTQATENG 
      EKPKPFFLF RISHYYYTG VGALVTIIC GLLISYVLE 
      NDDPPVHRD LISPVVHFA LPNDDDMQK EKNKDYYSV 
      EKALHLVTT NWEKNEDDA KIIKS
    CDS sequence
    • >TC003895 RA
      atggctggt ttaacgaca cttcttcca gatgttgcc 
      aaaatcgta ccgaaagtg tcgttctct tggtacgac 
      tatatcctt ttttgtgtc atgttgggt ctgagtgct 
      ttgatcggg gtgtatttc gggtgtttt ggcagcaag 
      cagaggtca gcagatgag tatttaatg ggcaataag 
      aagatgaaa gtgttcccg atttccgta tcactggtt 
      gccagccat gtttctggg atcactctt ttggcggta 
      cctgcggac atctaccgc tacggagcc tcctactgg 
      cttctgata atcgcggct attttcgtc accttcacc 
      acaatctac gtctatctc cctgtcttc tacaaactg 
      caaataacc agcacttac gagtacttg gaacgaaga 
      tttgacaac agaaatcgg atgtttgcg tcatttctt 
      ttcgcccta ggattgttt ttgtaccta ccaattgtt 
      atctacatc ccggcttta gccttatca gcggctacc 
      ggaatcaac atccattta ataactccg gtagtttgc 
      ggagtttgc attttttac accactctc ggaggtttg 
      aaagccgta gtttggacg gacaccctc caattcacc 
      atcacaaca ggggctgtc attgcgatt tttgtgatt 
      ggggtcaga gcgggcggc ggatttggg gccatctgg 
      tccaaagcc ctggaaggg catcgcctg gatattttt 
      gactttgac ccggacccc accaaacgg gactcgttc 
      tggattacg tccatcggc atgtccata ggatggctc 
      tcgatggtg gggattcac caaagttgc gtccagaag 
      ttcctctcc gtctccaca ttccaggaa tcaaaacag 
      tgtgttatt tacttttcg attggaata tccatcgtg 
      tcgtcattt agtgtcttc actggcctt atgatgtac 
      gcacgctac gccaactgt gaccccttc acaaccgga 
      actgtgcag aaaaacgac caacttttg ccctactat 
      gttatggac gttgcatct tatgttccg ggactttcg 
      ggacttttc attgctggg attttctgc gccgctttg 
      agcacttta tccgcttgt cttaactgt ttggcaggg 
      accatgtat gaggatttt atttcgaaa ataattggg 
      catgtggaa gagaaaacc acgacttat attttgaaa 
      ttgttggtg gttataacc ggagttgtg tgtacagtc 
      ctagtgttt gttattgag catcttgga gggatttta 
      gccctggct ataagtctg gcaggggtt acctcaggg 
      cctttgttg ggcatgttc acactaggg gttttgttc 
      ccgaaagcg tcaagtaaa ggttctctg tacggagca 
      attattgca ctacttttt gcttcatgg attgtcaca 
      atggcccag tactacaaa gcacaagga agcatagtt 
      gatgtgcca aaaccgacg tctgtggat ggttgtttg 
      gctaatttt acaacactt tcacctctt accacactt 
      aattccact ttatcatca ctggaaagt gtggcaaca 
      gaagatatt acgatagaa aactcgact ataagtcaa 
      ttactaagc aattttact caagcgacc gaaaatgga 
      gaaaaaccg aaaccattt tttttattt cgaatttca 
      cattactac tacaccgga gttggagcg ttggttaca 
      ataatatgc ggtttattg ataagttat gtgttggaa 
      aatgacgat cctccggta catcgcgat ttaataagt 
      ccggttgtt cattttgcc cttccgaat gatgatgat 
      atgcaaaaa gagaaaaac aaggattat tacagtgtg 
      gaaaaagcg ctgcatctt gtgacgacc aactgggaa 
      aaaaacgaa gacgatgcg aaaataata aaatcatga
    mRNA sequence
    • >TC003895 RA
      cgataaata agataagtt gagtaaacc ttctgagag 
      tatttatat gtctaagaa gaagaaacg ccacttcta 
      gtcaatcag aagaagtcg cgatggctg gtttaacga 
      cacttcttc cagatgttg ccaaaatcg taccgaaag 
      tgtcgttct cttggtacg actatatcc ttttttgtg 
      tcatgttgg gtctgagtg ctttgatcg gggtgtatt 
      tcgggtgtt ttggcagca agcagaggt cagcagatg 
      agtatttaa tgggcaata agaagatga aagtgttcc 
      cgatttccg tatcactgg ttgccagcc atgtttctg 
      ggatcactc ttttggcgg tacctgcgg acatctacc 
      gctacggag cctcctact ggcttctga taatcgcgg 
      ctattttcg tcaccttca ccacaatct acgtctatc 
      tccctgtct tctacaaac tgcaaataa ccagcactt 
      acgagtact tggaacgaa gatttgaca acagaaatc 
      ggatgtttg cgtcatttc ttttcgccc taggattgt 
      ttttgtacc taccaattg ttatctaca tcccggctt 
      tagccttat cagcggcta ccggaatca acatccatt 
      taataactc cggtagttt gcggagttt gcatttttt 
      acaccactc tcggaggtt tgaaagccg tagtttgga 
      cggacaccc tccaattca ccatcacaa caggggctg 
      tcattgcga tttttgtga ttggggtca gagcgggcg 
      gcggatttg gggccatct ggtccaaag ccctggaag 
      ggcatcgcc tggatattt ttgactttg acccggacc 
      ccaccaaac gggactcgt tctggatta cgtccatcg 
      gcatgtcca taggatggc tctcgatgg tggggattc 
      accaaagtt gcgtccaga agttcctct ccgtctcca 
      cattccagg aatcaaaac agtgtgtta tttactttt 
      cgattggaa tatccatcg tgtcgtcat ttagtgtct 
      tcactggcc ttatgatgt acgcacgct acgccaact 
      gtgacccct tcacaaccg gaactgtgc agaaaaacg 
      accaacttt tgccctact atgttatgg acgttgcat 
      cttatgttc cgggacttt cgggacttt tcattgctg 
      ggattttct gcgccgctt tgagcactt tatccgctt 
      gtcttaact gtttggcag ggaccatgt atgaggatt 
      ttatttcga aaataattg ggcatgtgg aagagaaaa 
      ccacgactt atattttga aattgttgg tggttataa 
      ccggagttg tgtgtacag tcctagtgt ttgttattg 
      agcatcttg gagggattt tagccctgg ctataagtc 
      tggcagggg ttacctcag ggcctttgt tgggcatgt 
      tcacactag gggttttgt tcccgaaag cgtcaagta 
      aaggttctc tgtacggag caattattg cactacttt 
      ttgcttcat ggattgtca caatggccc agtactaca 
      aagcacaag gaagcatag ttgatgtgc caaaaccga 
      cgtctgtgg atggttgtt tggctaatt ttacaacac 
      tttcacctc ttaccacac ttaattcca ctttatcat 
      cactggaaa gtgtggcaa cagaagata ttacgatag 
      aaaactcga ctataagtc aattactaa gcaatttta 
      ctcaagcga ccgaaaatg gagaaaaac cgaaaccat 
      tttttttat ttcgaattt cacattact actacaccg 
      gagttggag cgttggtta caataatat gcggtttat 
      tgataagtt atgtgttgg aaaatgacg atcctccgg 
      tacatcgcg atttaataa gtccggttg ttcattttg 
      cccttccga atgatgatg atatgcaaa aagagaaaa 
      acaaggatt attacagtg tggaaaaag cgctgcatc 
      ttgtgacga ccaactggg aaaaaaacg aagacgatg 
      cgaaaataa taaaatcat gaattttgt atgaaataa 
      acagttatt ttagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY