Status
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Gene information
General information
TC003870 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 23875868..23995874
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003870
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00631

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    remarks: animals lost, adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003870 RA
      MAVVNFAMS PLRRRNCFL VLAFVVFIP FCAFVLFSV 
      PDITSEQML IQRLAELQV KLQYLDSMY RARQEDLQQ 
      LTQHIDQIG IPNDNTTTF SFPTPELRP EIKQLIKNM 
      SGMHAATGL NPPIMLRLP SSYHFLPHL LDDPSSLRL 
      AYLMSKGRN GVSVVMGVP TVRREKQSY LMDTLQNLI 
      DGMNSAEAN DSLIVVFVA ETDLEYVLQ VAKEIEMRF 
      PMQVDSGLI EVLSPSPAY YPNLDKLRI TLGDPPERV 
      RWRSKQNLD FAFLMSYSQ PKGTFYLQL EDDILAKPN 
      YVTEMKKFA IEKIARKEP WFVLDFCQL GFIGKMFKS 
      AELPWLIQF FQMFYNDKP VDWLLDHLI FTKVCNWDK 
      NSNCKRDKA KMWVHYKPS LFQHIGTHS SLKGKVQKL 
      KDKQFGKVA LFFPHSNPD AEVVSGIKH YKQYTLERA 
      YLGETFFWG LLPSPGDQL IFKFSSPIW IKRFFFRSG 
      NAEHPSDKF YNTTVEVLP AEANQYGNS LNVTSDGYI 
      VVGKFDSVG IATGVPDES IGKIQALRL HVHSESDNW 
      AILSEIHIQ DELATR
    CDS sequence
    • >TC003870 RA
      atggcagtg gttaacttc gccatgtcc cctctacga 
      cgtcgcaac tgcttcctg gtgctcgca tttgtcgtc 
      ttcataccc ttctgcgcc ttcgtgctc ttctccgtg 
      ccagacata acatcggag caaatgttg atccagcgt 
      ctggccgag ctgcaagtg aagctccaa tacctggac 
      tccatgtac agagccaga caggaggac ctgcagcag 
      ctgactcag cacatcgac cagatcggt attcccaac 
      gacaacacg accactttc agctttcca acgcctgaa 
      cttcggcct gaaatcaaa cagctaatc aagaacatg 
      tcgggaatg catgccgca acagggctg aacccgccc 
      attatgctc cggttgccc tcttcctat cacttcctt 
      ccgcatctg ctcgacgac ccatcgagc ctacgactg 
      gcctatctg atgtccaag ggcaggaat ggggtgtcg 
      gtggtcatg ggagtgccc acggtgaga agggaaaag 
      cagagctat ctgatggac acgttgcag aatctgatt 
      gatgggatg aacagcgct gaggctaat gattcttta 
      atcgttgtt tttgttgcc gagactgat ctggaatac 
      gttcttcaa gtcgccaaa gaaattgaa atgaggttt 
      ccaatgcag gtggattcc ggtttaatc gaagtgctt 
      tcgccgtct ccggcctac tacccgaat ttagataag 
      ttgcggatc acgttaggg gatcccccg gaacgagtc 
      aggtggcga tctaagcaa aacttggat ttcgccttc 
      ctcatgtcc tactcgcag cctaaagga acgttttat 
      ctacaactt gaagacgat atcctcgcc aaacccaat 
      tacgttacc gaaatgaaa aagttcgcc atcgaaaaa 
      attgccagg aaggaaccg tggttcgtc ttggatttt 
      tgccagctt ggttttatt gggaagatg ttcaaaagt 
      gcggaactt ccgtggctc atccagttt tttcagatg 
      ttctacaac gataagccg gtcgactgg cttttggac 
      caccttatt ttcaccaag gtgtgcaat tgggacaag 
      aacagtaac tgtaagcgg gataaggcc aaaatgtgg 
      gtgcactac aaaccttcg cttttccaa catattgga 
      acacattcc tccctcaag ggaaaagtt cagaagctt 
      aaggacaaa cagttcggt aaagtggct cttttcttt 
      cctcacagc aaccctgat gcggaagtc gtctcagga 
      ataaagcat tacaaacag tacacgttg gagcgcgcg 
      tatttaggc gagacgttc ttctggggg ctgcttccg 
      tccccggga gaccagctc atatttaaa ttctcaagc 
      cccatttgg ataaaaagg ttcttcttc aggagcggt 
      aacgcggag catccttct gataagttc tataatacc 
      acggtggag gtgttaccc gctgaagcg aatcaatac 
      ggcaattcc ttgaacgtg acaagtgat ggctatatt 
      gttgtagga aaatttgac agcgtgggt atagccaca 
      ggcgttcca gacgaaagc ataggaaaa atccaagcg 
      ctgcgcctt catgtacat agtgaaagc gacaactgg 
      gccatcctg agtgaaatc cacatccag gacgagttg 
      gctaccagg tga
    mRNA sequence
    • >TC003870 RA
      ccttacata agtacgatc tgctatgcc gtagaaatt 
      cagtcactg actgctccg acggtcgtc aactccaag 
      tgcagctcg agccggtct tttcctagt tttctccgc 
      gcgatcaaa acattactc ctgagggct cctctggtg 
      atgagaggg ccgctgtgt gtgtgacag tgggacatg 
      gcagtggtt aacttcgcc atgtcccct ctacgacgt 
      cgcaactgc ttcctggtg ctcgcattt gtcgtcttc 
      atacccttc tgcgccttc gtgctcttc tccgtgcca 
      gacataaca tcggagcaa atgttgatc cagcgtctg 
      gccgagctg caagtgaag ctccaatac ctggactcc 
      atgtacaga gccagacag gaggacctg cagcagctg 
      actcagcac atcgaccag atcggtatt cccaacgac 
      aacacgacc actttcagc tttccaacg cctgaactt 
      cggcctgaa atcaaacag ctaatcaag aacatgtcg 
      ggaatgcat gccgcaaca gggctgaac ccgcccatt 
      atgctccgg ttgccctct tcctatcac ttccttccg 
      catctgctc gacgaccca tcgagccta cgactggcc 
      tatctgatg tccaagggc aggaatggg gtgtcggtg 
      gtcatggga gtgcccacg gtgagaagg gaaaagcag 
      agctatctg atggacacg ttgcagaat ctgattgat 
      gggatgaac agcgctgag gctaatgat tctttaatc 
      gttgttttt gttgccgag actgatctg gaatacgtt 
      cttcaagtc gccaaagaa attgaaatg aggtttcca 
      atgcaggtg gattccggt ttaatcgaa gtgctttcg 
      ccgtctccg gcctactac ccgaattta gataagttg 
      cggatcacg ttaggggat cccccggaa cgagtcagg 
      tggcgatct aagcaaaac ttggatttc gccttcctc 
      atgtcctac tcgcagcct aaaggaacg ttttatcta 
      caacttgaa gacgatatc ctcgccaaa cccaattac 
      gttaccgaa atgaaaaag ttcgccatc gaaaaaatt 
      gccaggaag gaaccgtgg ttcgtcttg gatttttgc 
      cagcttggt tttattggg aagatgttc aaaagtgcg 
      gaacttccg tggctcatc cagtttttt cagatgttc 
      tacaacgat aagccggtc gactggctt ttggaccac 
      cttattttc accaaggtg tgcaattgg gacaagaac 
      agtaactgt aagcgggat aaggccaaa atgtgggtg 
      cactacaaa ccttcgctt ttccaacat attggaaca 
      cattcctcc ctcaaggga aaagttcag aagcttaag 
      gacaaacag ttcggtaaa gtggctctt ttctttcct 
      cacagcaac cctgatgcg gaagtcgtc tcaggaata 
      aagcattac aaacagtac acgttggag cgcgcgtat 
      ttaggcgag acgttcttc tgggggctg cttccgtcc 
      ccgggagac cagctcata tttaaattc tcaagcccc 
      atttggata aaaaggttc ttcttcagg agcggtaac 
      gcggagcat ccttctgat aagttctat aataccacg 
      gtggaggtg ttacccgct gaagcgaat caatacggc 
      aattccttg aacgtgaca agtgatggc tatattgtt 
      gtaggaaaa tttgacagc gtgggtata gccacaggc 
      gttccagac gaaagcata ggaaaaatc caagcgctg 
      cgccttcat gtacatagt gaaagcgac aactgggcc 
      atcctgagt gaaatccac atccaggac gagttggct 
      accaggtga ccagagatg ggcggccgg gcatctctt 
      ttttaccct cacatccgt catcgctac atgctcgac 
      cgctcaccg tctgtgcac cctctgtga tatccgagt 
      cgacaaccc ccaaacacc cgcgtgcaa tatctgctt 
      atcgcaacc atggcaaca ctatcacag caattaccc 
      aatcttgat tgtttcgca ttgttccca ccgtgactt 
      aactaaagc cgtattcag cagattata agcttacta 
      cccccagct ttgaccaca attcgattg ttacccaag 
      gcctaatta attctcatt cgtcgacaa tcaatcacg 
      cattttact aataattgg cctagatct gctggatat 
      ttggacgtc ctggaagcc aaactcttt tagatctga 
      tttttgcca tcgagattt aactagtcc aagtctgga 
      ttttttgta aatagatat ttttccatg atttttaat 
      gctcagtat ttgtcaaac gcggacgct gtacacaac 
      acttgcttt tgcagcgaa aaactaatt ccccttctg 
      tcaatactt ttttaacag cggacgtgt ttcgacaca 
      aatctcagt taattccct caaataaac gttctacac 
      atacatgta aacagtgtt gatgtctga ttaaaaata 
      tctaaatgt taataattg tattcaagt gcgtgatat 
      taatttgac caatttttt acccgcatt ggttgcaga 
      agtaaagtc cggatttta gatataatt gttcaaaat 
      aaaatacaa gcttcagaa tgcgaccat tcaagtttg 
      aacaattat ttgagtggc ctgtttcac aaatatttt 
      atgcaacac taattaatg gagttttta aattttctc 
      taccggaaa ttccacaaa ttgctacca attataata 
      aggataata atgtttaaa cagtcaatg aataacaag 
      tgtgaaaat ccactgatt ggtaaaatt aattacata 
      attaaacgt tgttttaaa agaacaata tttcaattt 
      tctaattaa tttaatacc taattaaca tgatataaa 
      aaatcaaca acataaatt caaaataaa ttaaattat 
      tttagaaat ttaatgagt cattcagtt tcattggca 
      attttaaaa tagtcttat gaaaattat ccaatagaa 
      aaaataatc aaataaacg caatgaagt aatgattaa 
      tgaaccaat aataaacaa ctttgtcga ggaaaaaaa 
      ctaggaatg tttacttct acgttttta atttattta 
      tttcgagaa aggtgtctt aatttaatt gtttcactg 
      tttgttatg ttttatttt gttattcat tttattggt 
      atagtagtt tatcttttt ttttatttc tattatcaa 
      atctgtata agtccaaaa cagcttaag tgcaatttt 
      aatttatta ttatttata agagttgga aaatagact 
      gagcgactc gtgaaaatt tgattttct ttatgaaaa 
      ttggcttgt tgttagcat tctctacaa tatttagtt 
      tttgtttac ttttttgtt atgcgaaag gtaataaat 
      atctaactt ataaaaatc tgaaaactg acagcagcg 
      gtttttatt aaactaatt atccacata attatttaa 
      ccgttgtct acatatttt gtattgttt ttttgttat 
      gaaaaaggc cttaaaaat tatatatca agatagttt 
      aatttaatt taggacgaa ctcttaaag gaaagcaaa 
      cttcattcc ggagtattt taataatct actaaattc 
      acattttta tatttaaat tcgtgaaga tttagattt 
      ttttatgaa aattggctt atccccttc tcttcatat 
      ttaattttt gtttacttt tttaattat gaaaaggtc 
      tcaaaaatt aaacaccaa gatattttc agttcattt 
      agaactacg taatttata cctaataat tgattatgt 
      taagtgtaa atgtgatag tttatgtgg caataccga 
      acatagaga ggtcatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY