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Gene information
General information
TC003841 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid23: 351732..358670
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003841
    iBeetle screen iB_00628
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003841 RA
      MEESKRKGK DIPDNNHEG EKENNVENG HDNDQSDAL 
      PDAFGTKLI SNNVVACMP AEKYATHYN MNHKNRGLA 
      LIFNHEVFD IHNLKPRTG TDQDRKNLE DVLRKLGFE 
      VIVFQDLRY HDIERQIKQ AAKYDHTDS DCLCISVLT 
      HGDTGILYA RDTQYKPDL LWSYFAADR CPSLAGKPK 
      IFFLQACQG DKLDGGVIL SRTETDGHL EIDSYRIPA 
      QADFLVVYS TVKGYYSWR NTTRGSWFI QALCEELRN 
      KGFDYDLGT ILTFVSQRV AFDFESNCP DSPTMHQQK 
      QIPCVMSML TRLIQFTRV KEGLEENET LQKQEKRKN 
      TIKGLFTKG K
    • >TC003841 RB
      MEESKRKGK DIPDNNHEG EKENNVENG HDNDQSDAL 
      PDAFGTKLI SNNVVACMP AEKYATHYN MNHKNRGLA 
      LIFNHEVFD IHNLKPRTG TDQDRKNLE DVLRKLGFE 
      VIVFQDLRY HDIERQIKQ AAKYDHTDS DCLCISVLT 
      HGDTGILYA RDTQYKPDL LWSYFAADR CPSLAGKPK 
      IFFLQACQG DKLDGGVIL SRTETDGHL EIDSYRIPA 
      QADFLVVYS TVKGYYSWR NTTRGSWFI QALCEELRN 
      KGFDYDLGT ILTFVSQRV AFDFESNCP DSPTMHQQK 
      QIPCVMSML TRLIQFTRV KEG
    CDS sequence
    • >TC003841 RA
      atggaggag agtaaacgc aaagggaaa gacattcct 
      gataacaac cacgaaggt gaaaaggaa aataacgtg 
      gagaatggc catgacaac gaccaaagt gatgcttta 
      cctgatgcg ttcggtact aagctgatt tcgaataat 
      gtggtggcg tgtatgcca gctgaaaaa tacgccaca 
      cattacaat atgaatcat aagaaccgc ggtttggcc 
      ttaattttc aaccatgaa gttttcgat attcataat 
      ttgaaacca aggactggg actgatcaa gaccgaaaa 
      aatttggaa gatgttttg aggaaactt ggctttgag 
      gtcattgtc tttcaagat ttgagatat cacgatatc 
      gaacgacag atcaaacag gctgccaaa tatgatcac 
      accgattcc gattgcttg tgcatttcc gttctcact 
      catggcgac accggaatt ctctacgcc agagacaca 
      caatacaaa cctgatttg ctctggagt tattttgca 
      gccgatcgg tgcccctct ctagccggc aaaccaaaa 
      attttcttc ctgcaagct tgccaaggc gataaattg 
      gacggtggt gttatttta tcaagaact gaaactgat 
      ggtcacctg gaaattgat tcgtatcgc attccagca 
      caggctgat ttcctcgtg gtttattca actgtaaaa 
      ggttattat tcgtggcgc aacaccact cgaggctcg 
      tggtttatc caagccttg tgtgaagag ctgcgaaat 
      aaaggcttt gattacgat ttaggcaca atcctaaca 
      tttgtttcc caacgtgtg gctttcgat tttgagagt 
      aattgtccc gattcgcca acaatgcac caacaaaaa 
      cagattcct tgcgtgatg agtatgctc acaaggttg 
      atacagttc acaagggtg aaagaagga ttggaagaa 
      aatgaaacg ctccaaaaa caagaaaaa cgtaaaaat 
      actattaag ggattattt acaaagggt aaataa
    • >TC003841 RB
      atggaggag agtaaacgc aaagggaaa gacattcct 
      gataacaac cacgaaggt gaaaaggaa aataacgtg 
      gagaatggc catgacaac gaccaaagt gatgcttta 
      cctgatgcg ttcggtact aagctgatt tcgaataat 
      gtggtggcg tgtatgcca gctgaaaaa tacgccaca 
      cattacaat atgaatcat aagaaccgc ggtttggcc 
      ttaattttc aaccatgaa gttttcgat attcataat 
      ttgaaacca aggactggg actgatcaa gaccgaaaa 
      aatttggaa gatgttttg aggaaactt ggctttgag 
      gtcattgtc tttcaagat ttgagatat cacgatatc 
      gaacgacag atcaaacag gctgccaaa tatgatcac 
      accgattcc gattgcttg tgcatttcc gttctcact 
      catggcgac accggaatt ctctacgcc agagacaca 
      caatacaaa cctgatttg ctctggagt tattttgca 
      gccgatcgg tgcccctct ctagccggc aaaccaaaa 
      attttcttc ctgcaagct tgccaaggc gataaattg 
      gacggtggt gttatttta tcaagaact gaaactgat 
      ggtcacctg gaaattgat tcgtatcgc attccagca 
      caggctgat ttcctcgtg gtttattca actgtaaaa 
      ggttattat tcgtggcgc aacaccact cgaggctcg 
      tggtttatc caagccttg tgtgaagag ctgcgaaat 
      aaaggcttt gattacgat ttaggcaca atcctaaca 
      tttgtttcc caacgtgtg gctttcgat tttgagagt 
      aattgtccc gattcgcca acaatgcac caacaaaaa 
      cagattcct tgcgtgatg agtatgctc acaaggttg 
      atacagttc acaagggtg aaagaaggg tag
    mRNA sequence
    • >TC003841 RA
      ttattgttt tggcgtttg ttgtgaaca acgtgcgtg 
      cgtttgagt gtcttcaaa gaaaattgt gatttttca 
      acgatggag gagagtaaa cgcaaaggg aaagacatt 
      cctgataac aaccacgaa ggtgaaaag gaaaataac 
      gtggagaat ggccatgac aacgaccaa agtgatgct 
      ttacctgat gcgttcggt actaagctg atttcgaat 
      aatgtggtg gcgtgtatg ccagctgaa aaatacgcc 
      acacattac aatatgaat cataagaac cgcggtttg 
      gccttaatt ttcaaccat gaagttttc gatattcat 
      aatttgaaa ccaaggact gggactgat caagaccga 
      aaaaatttg gaagatgtt ttgaggaaa cttggcttt 
      gaggtcatt gtctttcaa gatttgaga tatcacgat 
      atcgaacga cagatcaaa caggctgcc aaatatgat 
      cacaccgat tccgattgc ttgtgcatt tccgttctc 
      actcatggc gacaccgga attctctac gccagagac 
      acacaatac aaacctgat ttgctctgg agttatttt 
      gcagccgat cggtgcccc tctctagcc ggcaaacca 
      aaaattttc ttcctgcaa gcttgccaa ggcgataaa 
      ttggacggt ggtgttatt ttatcaaga actgaaact 
      gatggtcac ctggaaatt gattcgtat cgcattcca 
      gcacaggct gatttcctc gtggtttat tcaactgta 
      aaaggttat tattcgtgg cgcaacacc actcgaggc 
      tcgtggttt atccaagcc ttgtgtgaa gagctgcga 
      aataaaggc tttgattac gatttaggc acaatccta 
      acatttgtt tcccaacgt gtggctttc gattttgag 
      agtaattgt cccgattcg ccaacaatg caccaacaa 
      aaacagatt ccttgcgtg atgagtatg ctcacaagg 
      ttgatacag ttcacaagg gtgaaagaa ggattggaa 
      gaaaatgaa acgctccaa aaacaagaa aaacgtaaa 
      aatactatt aagggatta tttacaaag ggtaaataa 
      atgcgtaat tacttaatt cgattaatt ttaacatat 
      ttgtgcaca ggtgtcttt caaattttt agcttttta 
      aattttatt actaaaagt aggtctaag aaatagtga 
      tgtttgttt tgtaggaaa ttttcactt tttagtaaa 
      ttattttgg ctgttgtat cattccaaa tgcacaagt 
      atgtgcagt gcaatcgtt tgattattt ttaagtaaa 
      tgttatgcg tagtaagtg ccaatttat gtgttgtgg 
      ctgtaaaaa ttagattgg ccacactgt atcattcag 
      tcagtgttg ccaacagga ttttaaggt cacaggcca 
      cttgatagt ttagttgta tatactttg aaccagttg 
      taagccaag caccaatga caacgtgtt ggcgtatgt 
      aattaaggc acatgagaa atatcacac tgtattaaa 
      tcgttagtt taatttttg catatttaa tatacgtgc 
      cattgtctt tcaaaaaag tgtcttatg gaagtcaag 
      tgtgttaac gattggaat ggataaact acagctgtg 
      taaacattg ccattttaa aaatattgt attactaca 
      taattagtt ggtgtattt ttacatgta aatgttttt 
      atattgtat acaccttta tgttttaca aaagaataa 
      aagatgtac attgtg
    • >TC003841 RB
      ttattgttt tggcgtttg ttgtgaaca acgtgcgtg 
      cgtttgagt gtcttcaaa gaaaattgt gatttttca 
      acgatggag gagagtaaa cgcaaaggg aaagacatt 
      cctgataac aaccacgaa ggtgaaaag gaaaataac 
      gtggagaat ggccatgac aacgaccaa agtgatgct 
      ttacctgat gcgttcggt actaagctg atttcgaat 
      aatgtggtg gcgtgtatg ccagctgaa aaatacgcc 
      acacattac aatatgaat cataagaac cgcggtttg 
      gccttaatt ttcaaccat gaagttttc gatattcat 
      aatttgaaa ccaaggact gggactgat caagaccga 
      aaaaatttg gaagatgtt ttgaggaaa cttggcttt 
      gaggtcatt gtctttcaa gatttgaga tatcacgat 
      atcgaacga cagatcaaa caggctgcc aaatatgat 
      cacaccgat tccgattgc ttgtgcatt tccgttctc 
      actcatggc gacaccgga attctctac gccagagac 
      acacaatac aaacctgat ttgctctgg agttatttt 
      gcagccgat cggtgcccc tctctagcc ggcaaacca 
      aaaattttc ttcctgcaa gcttgccaa ggcgataaa 
      ttggacggt ggtgttatt ttatcaaga actgaaact 
      gatggtcac ctggaaatt gattcgtat cgcattcca 
      gcacaggct gatttcctc gtggtttat tcaactgta 
      aaaggttat tattcgtgg cgcaacacc actcgaggc 
      tcgtggttt atccaagcc ttgtgtgaa gagctgcga 
      aataaaggc tttgattac gatttaggc acaatccta 
      acatttgtt tcccaacgt gtggctttc gattttgag 
      agtaattgt cccgattcg ccaacaatg caccaacaa 
      aaacagatt ccttgcgtg atgagtatg ctcacaagg 
      ttgatacag ttcacaagg gtgaaagaa gggtagggt 
      tctgtatta ccgacttta ccatagcat gtatttcta 
      tcaagtagc tgtattaag tttaatgtt gagctactt 
      gctcattcc atttatatt ccgatctct cttagtttt 
      gttttttgc tcaatgcgc gtctaaata gtctaaacg 
      atgtgttct tgaagactg ccaaacatg accttaaac 
      gtaattttt agattggaa gaaaatgaa acgctccaa 
      aaacaagaa aaacgtaaa aatactatt aagggatta 
      tttacaaag ggtaaataa atgcgtaat tacttaatt 
      cgattaatt ttaacatat ttgtgcaca ggtgtcttt 
      caaattttt agcttttta aattttatt actaaaagt 
      aggtctaag aaatagtga tgtttgttt tgtaggaaa 
      ttttcactt tttagtaaa ttattttgg ctgttgtat 
      cattccaaa tgcacaagt atgtgcagt gcaatcgtt 
      tgattattt ttaagtaaa tgttatgcg tagtaagtg 
      ccaatttat gtgttgtgg ctgtaaaaa ttagattgg 
      ccacactgt atcattcag tcagtgttg ccaacagga 
      ttttaaggt cacaggcca cttgatagt ttagttgta 
      tatactttg aaccagttg taagccaag caccaatga 
      caacgtgtt ggcgtatgt aattaaggc acatgagaa 
      atatcacac tgtattaaa tcgttagtt taatttttg 
      catatttaa tatacgtgc cattgtctt tcaaaaaag 
      tgtcttatg gaagtcaag tgtgttaac gattggaat 
      ggataaact acagctgtg taaacattg ccattttaa 
      aaatattgt attactaca taattagtt ggtgtattt 
      ttacatgta aatgttttt atattgtat acaccttta 
      tgttttaca aaagaataa aagatgtac attgtg