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Gene information
General information
TC032573
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003839,TC003840
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid23: 341048..345400
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032573
    iBeetle screen iB_00627
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032573 RA
      MSSQNLDFH NKIAKLCIE HFDSLPKTG KPKENEWTI 
      LSCIVLEDS EKYEVVALG TGTKCIGQD KMSRDGDIL 
      NDSHAEVIC RRAFLRFIY DSILSQHTF LYEPEKQIF 
      TFKPNIKFH FFSTHVPCG DAAIFAIQN VEDFGDLIQ 
      VSVQNSLKR TAEECDSDS GSETKRIKL TDNIFRTGA 
      KCLKTDVKQ DLKLQGSEY HVTGVVRTK PGRGSPTLS 
      VSCSDKLAK WCHLGLQGA LLGFLLNKP IYFSSFTIA 
      GGTPFNEEA MRRALFDRL GTPLLEEPY HRSDVTLGQ 
      ADVKFLFEK FGNKNPCPS SIIWSKGST QCEVAIDGR 
      RQGVTKKHL KTVGALKIC KLKLFETLV KIVDQFKIK 
      IENVDDLSK LTYQEAKLC AKDYGRAWT SLKNYFNTK 
      SLHKKMDCK SLKVQLPSG KYMPLIGFG TFQIRGRNL 
      IRDVLNHAL AAGYRQIDT AAVYGNEQD IGIALKELL 
      PKYKLTRED IFITSKLSP SDQGDKALQ ALKTSIQNL 
      DCTYLDLYL IHWPGAHGI PGSHPNNPK LRAASWSSL 
      SQGVQQGLV RDIGVSNYT TRHLTELLA TNPSVKPAV 
      NQVEWHPHC HQSDLKEFC KKEGILLQA YSSLGGSNN 
      PNLISDPIV CEIAKKLNK SPAQVLLRW ALQQDIGII 
      PKARSKEHI EANIDLNFA IPQEDMSRL SNIKTQEKY 
      AWDPKTIQ
    CDS sequence
    • >TC032573 RA
      atgtcatca caaaatttg gacttccac aacaaaata 
      gcgaaactt tgcattgaa cattttgac tccttgcca 
      aaaacagga aagcccaag gaaaatgaa tggacgata 
      ttgtcttgt attgtctta gaagacagc gaaaagtac 
      gaagtagtg gcattagga acgggaaca aagtgcata 
      ggacaagac aagatgtct cgagatggg gacattctt 
      aacgactcc catgctgaa gttatctgc cgtcgagcc 
      ttcctccga tttatctac gacagtatt ttgtcccag 
      catacgttt ttgtatgaa ccggaaaaa caaattttt 
      acttttaaa cccaacata aaattccac tttttctca 
      acccacgtt ccctgtggg gatgctgcg attttcgca 
      atacaaaat gtggaagat tttggcgat ttgattcaa 
      gtttcggtc caaaattcg ctaaaaagg acggcagaa 
      gaatgtgat agtgatagt ggtagtgaa acaaaaaga 
      attaaatta acagacaat atttttaga actggggca 
      aaatgtttg aagacggat gtgaaacaa gacttgaaa 
      ttgcaaggg tcagagtat cacgtgacg ggggttgtg 
      cgcactaaa ccaggaagg ggctcccca actttgtcc 
      gtttcttgc agcgataaa ctggcaaag tggtgtcat 
      ttggggctc cagggggcc ttgttgggt tttctgtta 
      aacaaacct atttatttt agtagtttt acgattgct 
      gggggtact ccttttaat gaagaggca atgagaagg 
      gcgctgttt gacagatta gggaccccc cttctggaa 
      gaaccttat cacagaagt gatgttacc ttaggacag 
      gccgatgtc aaatttttg tttgaaaaa tttggtaac 
      aaaaatcct tgcccttcc agtattatt tggtcaaaa 
      ggatccacg caatgtgag gttgccatt gatggcaga 
      agacaagga gtgacaaag aaacaccta aaaacagtt 
      ggtgctcta aaaatttgc aagttaaaa ttgtttgaa 
      actttggta aaaattgtt gaccaattt aaaatcaaa 
      atagaaaat gttgatgat ttaagtaag ctaacgtat 
      caagaagca aaattgtgt gcaaaagat tatggcaga 
      gcttggact agtcttaaa aattatttt aacacgaaa 
      agcttgcac aagaagatg gactgtaaa tcactgaaa 
      gtccaactt cccagtgga aaatacatg cctttaatt 
      ggatttggc actttccaa atccggggc cgcaattta 
      ataagagac gtcctaaac catgccctt gctgccggt 
      tacagacaa atagacaca gcagctgtg tatggcaat 
      gaacaagac attggaatc gccctaaaa gaactcttg 
      cccaagtat aaactaaca agggaagac attttcata 
      acatcaaag ctttcccca agtgaccaa ggcgataaa 
      gccctccag gccctcaaa acctcaatc caaaacctg 
      gattgcaca tacctagac ctctaccta atccactgg 
      cctggggca catggcatt ccaggcagc cacccaaac 
      aaccccaaa ctccgggca gcaagctgg agttctctt 
      agccaaggt gtccaacaa ggactagtg cgagacatt 
      ggagtctca aactacacc acacgacac ctgaccgag 
      ctcctggcc actaacccc tcagttaaa cccgcagtt 
      aatcaggtg gagtggcac ccacattgt caccaaagc 
      gacctgaag gagttttgc aaaaaagag gggatttta 
      ctccaagct tattcctca ctcgggggc tcaaataac 
      cccaacttg atctcagat cccatcgtg tgcgaaatt 
      gccaaaaag ctgaacaag agcccagct caggttttg 
      ctacgttgg gccctccaa caagacatt ggcattatt 
      ccaaaagct aggtccaag gagcacatt gaggcaaat 
      attgatctt aactttgcc attcctcag gaagatatg 
      agcaggctg agtaacatt aaaacgcaa gaaaagtac 
      gcttgggac ccgaaaact attcaataa
    mRNA sequence
    • >TC032573 RA
      ctttacttc taaacaact atttaaatt gtctttata 
      aatgttgcg tgtgtagtt taatcaatg aaatcacta 
      attccccac aagtgagcc accatgtca tcacaaaat 
      ttggacttc cacaacaaa atagcgaaa ctttgcatt 
      gaacatttt gactccttg ccaaaaaca ggaaagccc 
      aaggaaaat gaatggacg atattgtct tgtattgtc 
      ttagaagac agcgaaaag tacgaagta gtggcatta 
      ggaacggga acaaagtgc ataggacaa gacaagatg 
      tctcgagat ggggacatt cttaacgac tcccatgct 
      gaagttatc tgccgtcga gccttcctc cgatttatc 
      tacgacagt attttgtcc cagcatacg tttttgtat 
      gaaccggaa aaacaaatt tttactttt aaacccaac 
      ataaaattc cactttttc tcaacccac gttccctgt 
      ggggatgct gcgattttc gcaatacaa aatgtggaa 
      gattttggc gatttgatt caagtttcg gtccaaaat 
      tcgctaaaa aggacggca gaagaatgt gatagtgat 
      agtggtagt gaaacaaaa agaattaaa ttaacagac 
      aatattttt agaactggg gcaaaatgt ttgaagacg 
      gatgtgaaa caagacttg aaattgcaa gggtcagag 
      tatcacgtg acgggggtt gtgcgcact aaaccagga 
      aggggctcc ccaactttg tccgtttct tgcagcgat 
      aaactggca aagtggtgt catttgggg ctccagggg 
      gccttgttg ggttttctg ttaaacaaa cctatttat 
      tttagtagt tttacgatt gctgggggt actcctttt 
      aatgaagag gcaatgaga agggcgctg tttgacaga 
      ttagggacc ccccttctg gaagaacct tatcacaga 
      agtgatgtt accttagga caggccgat gtcaaattt 
      ttgtttgaa aaatttggt aacaaaaat ccttgccct 
      tccagtatt atttggtca aaaggatcc acgcaatgt 
      gaggttgcc attgatggc agaagacaa ggagtgaca 
      aagaaacac ctaaaaaca gttggtgct ctaaaaatt 
      tgcaagtta aaattgttt gaaactttg gtaaaaatt 
      gttgaccaa tttaaaatc aaaatagaa aatgttgat 
      gatttaagt aagctaacg tatcaagaa gcaaaattg 
      tgtgcaaaa gattatggc agagcttgg actagtctt 
      aaaaattat tttaacacg aaaagcttg cacaagaag 
      atggactgt aaatcactg aaagtccaa cttcccagt 
      ggaaaatac atgccttta attggattt ggcactttc 
      caaatccgg ggccgcaat ttaataaga gacgtccta 
      aaccatgcc cttgctgcc ggttacaga caaatagac 
      acagcagct gtgtatggc aatgaacaa gacattgga 
      atcgcccta aaagaactc ttgcccaag tataaacta 
      acaagggaa gacattttc ataacatca aagctttcc 
      ccaagtgac caaggcgat aaagccctc caggccctc 
      aaaacctca atccaaaac ctggattgc acataccta 
      gacctctac ctaatccac tggcctggg gcacatggc 
      attccaggc agccaccca aacaacccc aaactccgg 
      gcagcaagc tggagttct cttagccaa ggtgtccaa 
      caaggacta gtgcgagac attggagtc tcaaactac 
      accacacga cacctgacc gagctcctg gccactaac 
      ccctcagtt aaacccgca gttaatcag gtggagtgg 
      cacccacat tgtcaccaa agcgacctg aaggagttt 
      tgcaaaaaa gaggggatt ttactccaa gcttattcc 
      tcactcggg ggctcaaat aaccccaac ttgatctca 
      gatcccatc gtgtgcgaa attgccaaa aagctgaac 
      aagagccca gctcaggtt ttgctacgt tgggccctc 
      caacaagac attggcatt attccaaaa gctaggtcc 
      aaggagcac attgaggca aatattgat cttaacttt 
      gccattcct caggaagat atgagcagg ctgagtaac 
      attaaaacg caagaaaag tacgcttgg gacccgaaa 
      actattcaa taattttta tttttaata aagttgttt 
      aataaag