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Gene information
General information
TC003826 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 2532436..2533896
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003826
    iBeetle screen iB_00623
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003826 RA
      MIKSGTFKS LLRRHKNVL HFCQLSSAA QPQSDPKSH 
      SFRTDENDP LKHTLDHVG QYYRVPQNE CKVYLYGGL 
      PKSYEIQTK TFNETCLMV RKPSVDIIN CLKGLDYTK 
      PAVRFVLFG KKGSGKSLT MAHVLHYAQ KAGFLLVHV 
      PWLGNWMRN PKELSSSET REGFYDLNL DAAAWLVHF 
      KSQNEHLLS APELKTTRE HVWSKREST PKDSSLLEL 
      IDHGINRLK YASECVLAL AEELKQLTK AGNCKTLVA 
      IDGFNAFFY PKTRVFKEK KEVVPPNKI TLTEAFLSL 
      TKFDWNNAA IVVTVDEIA IAEEDHKSH FPRYLLGKE 
      GFEHLDPFV PVFVDAYSP KELTSCMDY YRERKWVRY 
      FPQQDEELA SLSAYNPYK LMKLCISL
    CDS sequence
    • >TC003826 RA
      atgatcaaa tccggcacg ttcaagtcc ctgttgaga 
      cgccacaaa aacgttttg cacttttgc caattgtct 
      tcagccgcc caaccacag tcagacccc aaaagccac 
      agttttcgt accgatgag aacgacccc cttaaacac 
      acccttgat catgtgggg cagtactac agagtcccc 
      caaaatgag tgcaaagta tatctatac gggggttta 
      cccaaatcg tacgaaatc caaacaaag accttcaat 
      gaaacttgt ttaatggtg aggaaacct tcagtcgat 
      attataaat tgtttgaaa gggcttgat tacacaaaa 
      cccgccgta cgttttgtc ctatttggt aaaaaagga 
      agtggcaag agtttgaca atggcacat gtgttacac 
      tacgcccaa aaggccggt tttttgctg gtccatgtc 
      ccttggctg ggcaattgg atgcggaac cctaaggaa 
      ctgagcagc tctgagact agagaaggg ttttatgat 
      ttaaatctg gacgcggct gcgtggttg gtgcacttt 
      aagtcgcag aatgagcat ttgctgagc gcccctgag 
      cttaaaacg actcgggag catgtttgg agtaaacgt 
      gagagtaca ccaaaagat tctagtttg cttgaattg 
      attgatcat ggcattaat cggctgaaa tatgcctca 
      gagtgtgtt cttgcccta gctgaagaa ttgaaacaa 
      ttgactaaa gctggaaat tgtaaaact ctagtggca 
      attgatggt ttcaatgcg tttttctac ccaaaaact 
      cgtgttttt aaggagaag aaggaagtg gttccgcca 
      aataaaatc acactgact gaggctttt ctcagtttg 
      accaaattt gattggaat aatgcggca atcgttgtc 
      acagttgat gaaattgcc attgctgag gaagatcac 
      aagtcgcat tttccaagg tatctcttg ggtaaggaa 
      ggatttgaa catttggac ccttttgtg cccgttttt 
      gttgacgct tattcacca aaagagctc acaagttgt 
      atggattat tacagagaa aggaagtgg gttaggtat 
      ttcccacaa caggatgaa gaattggcg tcactaagt 
      gcatataat ccttataaa ttaatgaag ttatgcata 
      tcgctttaa
    mRNA sequence
    • >TC003826 RA
      tgtttcaac attgttctt tcgaatcaa acattcaac 
      tctcgaaat aattttatt tatgaaaca caattgaag 
      taaaacgaa ctttttcac cgtcttatc tgctacgaa 
      actaaaatt aaaatgatc aaatccggc acgttcaag 
      tccctgttg agacgccac aaaaacgtt ttgcacttt 
      tgccaattg tcttcagcc gcccaacca cagtcagac 
      cccaaaagc cacagtttt cgtaccgat gagaacgac 
      ccccttaaa cacaccctt gatcatgtg gggcagtac 
      tacagagtc ccccaaaat gagtgcaaa gtatatcta 
      tacgggggt ttacccaaa tcgtacgaa atccaaaca 
      aagaccttc aatgaaact tgtttaatg gtgaggaaa 
      ccttcagtc gatattata aattgtttg aaagggctt 
      gattacaca aaacccgcc gtacgtttt gtcctattt 
      ggtaaaaaa ggaagtggc aagagtttg acaatggca 
      catgtgtta cactacgcc caaaaggcc ggttttttg 
      ctggtccat gtcccttgg ctgggcaat tggatgcgg 
      aaccctaag gaactgagc agctctgag actagagaa 
      gggttttat gatttaaat ctggacgcg gctgcgtgg 
      ttggtgcac tttaagtcg cagaatgag catttgctg 
      agcgcccct gagcttaaa acgactcgg gagcatgtt 
      tggagtaaa cgtgagagt acaccaaaa gattctagt 
      ttgcttgaa ttgattgat catggcatt aatcggctg 
      aaatatgcc tcagagtgt gttcttgcc ctagctgaa 
      gaattgaaa caattgact aaagctgga aattgtaaa 
      actctagtg gcaattgat ggtttcaat gcgtttttc 
      tacccaaaa actcgtgtt tttaaggag aagaaggaa 
      gtggttccg ccaaataaa atcacactg actgaggct 
      tttctcagt ttgaccaaa tttgattgg aataatgcg 
      gcaatcgtt gtcacagtt gatgaaatt gccattgct 
      gaggaagat cacaagtcg cattttcca aggtatctc 
      ttgggtaag gaaggattt gaacatttg gaccctttt 
      gtgcccgtt tttgttgac gcttattca ccaaaagag 
      ctcacaagt tgtatggat tattacaga gaaaggaag 
      tgggttagg tatttccca caacaggat gaagaattg 
      gcgtcacta agtgcatat aatccttat aaattaatg 
      aagttatgc atatcgctt taaatgtaa cttaattag 
      agtaaataa aattgaatt acagta