Status
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Gene information
General information
TC003823 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 21948258..22115865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003823
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00622

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003823 RA
      MKEFVLITC MLFFQHQLK PLHAAPPIE ACPHDSVLV 
      EDTCKCNYE SCSKPPCPY ELTIAVNGT DVPGTCCPS 
      YTCEGCPND TLIDGKCPC APDATLDSK NECKCVDPH 
      KKLHNGECI CDQDLCELP QLCDVNYVS VTEQNGCCV 
      TTKCIKCPD DSFATSNKN GEIEDKCVC YQCPNKECG 
      PSEKVVIVQ HGKGFPGSC CDMYRCEKD ETEKKCLVD 
      GVWYGEGDI WNTTNTQVC KCQDGLSFC WNSKDEEIF 
      RPCHKEGKM YKHDESWML DSCTNCTCF NGETKCIAH 
      TCEVSQGHI DKPECQALN CSKICVDGY KLNKRGCPI 
      CKCNSSYKY DDILHKYNI TKNELREIL EDYFAHNRT 
      ISTTTTVDP VGSASGDSL EDDERFTTP VTELDENSQ 
      LVTWIVPTI ILALLVGTL VILGFRWWC CSKKGRKET 
      KTARSTTYE RIKLTKPTM FNNNYIGNN NKDEQNNVK 
      NIKGSV
    CDS sequence
    • >TC003823 RA
      atgaaggaa tttgttcta atcacctgc atgctattt 
      tttcaacat caacttaaa ccgctacac gccgctcca 
      cctatcgaa gcctgcccg cacgacagc gtcctcgtc 
      gaagacacc tgcaagtgc aactacgag tcgtgctcc 
      aaaccccca tgtccttac gaactcacc atcgccgtt 
      aacggaacg gacgttccc ggcacctgc tgtccgtcc 
      tatacctgc gagggttgc cccaatgac accctcatc 
      gacggcaaa tgcccttgc gcccccgac gccacactc 
      gactccaaa aacgagtgc aaatgcgtc gaccctcac 
      aaaaaactc cacaacggg gaatgtatt tgcgaccag 
      gatttgtgc gagctaccg cagctctgc gacgtgaac 
      tacgtctcc gtcacggag cagaacgga tgctgcgtt 
      acgacgaag tgtattaag tgtcctgac gacagcttc 
      gctacgagt aataagaac ggggagatt gaggataaa 
      tgcgtgtgt tatcagtgc ccgaataag gagtgcggg 
      ccttcggaa aaagttgtg atagttcaa catggaaag 
      gggtttccg ggaagttgc tgtgatatg tataggtgt 
      gagaaagac gagacggag aagaagtgt ctcgtggac 
      ggcgtatgg tatggagaa ggtgacatt tggaacaca 
      acgaacacg caagtgtgt aaatgtcag gatggtctt 
      tccttctgc tggaactca aaagacgag gagattttc 
      cgaccttgc cacaaagaa ggcaaaatg tacaaacac 
      gacgagtct tggatgctt gatagttgc actaactgt 
      acttgtttc aatggagaa actaaatgt atagcacac 
      acttgtgaa gtgtcgcaa ggacatatc gacaaacct 
      gaatgccaa gcgttgaac tgcagcaag atttgcgtg 
      gatggatat aaattgaat aagagaggt tgtccgatt 
      tgcaaatgc aattcgtcg tataagtac gacgatatt 
      ttgcacaaa tataatatc actaagaat gaattgagg 
      gagattctt gaggattat ttcgcacac aatcgaact 
      atttcgact acgacgacg gtggatccg gttgggtca 
      gccagtggt gacagtttg gaggatgac gagcggttc 
      acaactcct gtaactgaa ttagatgaa aactcccaa 
      ctagttaca tggattgtg ccaacaata attttggcg 
      ctattggtg ggaacgcta gtaatactt ggatttcgt 
      tggtggtgt tgttcgaag aaaggaaga aaagaaacg 
      aaaacagcc cgatctacc acttacgag cgaataaaa 
      ttaacgaaa cctacgatg tttaacaac aactatatc 
      ggcaataat aacaaagac gaacagaat aatgtgaaa 
      aatattaaa ggtagtgtg tga
    mRNA sequence
    • >TC003823 RA
      gtcgtgttg ttgccttgt tttagtcgc taatcgcgg 
      tgtatgctg tacggtcat ctaccaggt gttgcttct 
      aacctcaaa cttttacgc gcttgacag tttttccaa 
      attgtcttt taccgcttt tacgtcgag cgtaattat 
      gtccaaaat acgcattga cagactgat ttcgtcgat 
      ttttgtgaa cttgttttg cgcgtaatt ctcccagtg 
      gtgccttta tgactgttt cttcgccga tacgcaaaa 
      tgaaggaat ttgttctaa tcacctgca tgctatttt 
      ttcaacatc aacttaaac cgctacacg ccgctccac 
      ctatcgaag cctgcccgc acgacagcg tcctcgtcg 
      aagacacct gcaagtgca actacgagt cgtgctcca 
      aacccccat gtccttacg aactcacca tcgccgtta 
      acggaacgg acgttcccg gcacctgct gtccgtcct 
      atacctgcg agggttgcc ccaatgaca ccctcatcg 
      acggcaaat gcccttgcg cccccgacg ccacactcg 
      actccaaaa acgagtgca aatgcgtcg accctcaca 
      aaaaactcc acaacgggg aatgtattt gcgaccagg 
      atttgtgcg agctaccgc agctctgcg acgtgaact 
      acgtctccg tcacggagc agaacggat gctgcgtta 
      cgacgaagt gtattaagt gtcctgacg acagcttcg 
      ctacgagta ataagaacg gggagattg aggataaat 
      gcgtgtgtt atcagtgcc cgaataagg agtgcgggc 
      cttcggaaa aagttgtga tagttcaac atggaaagg 
      ggtttccgg gaagttgct gtgatatgt ataggtgtg 
      agaaagacg agacggaga agaagtgtc tcgtggacg 
      gcgtatggt atggagaag gtgacattt ggaacacaa 
      cgaacacgc aagtgtgta aatgtcagg atggtcttt 
      ccttctgct ggaactcaa aagacgagg agattttcc 
      gaccttgcc acaaagaag gcaaaatgt acaaacacg 
      acgagtctt ggatgcttg atagttgca ctaactgta 
      cttgtttca atggagaaa ctaaatgta tagcacaca 
      cttgtgaag tgtcgcaag gacatatcg acaaacctg 
      aatgccaag cgttgaact gcagcaaga tttgcgtgg 
      atggatata aattgaata agagaggtt gtccgattt 
      gcaaatgca attcgtcgt ataagtacg acgatattt 
      tgcacaaat ataatatca ctaagaatg aattgaggg 
      agattcttg aggattatt tcgcacaca atcgaacta 
      tttcgacta cgacgacgg tggatccgg ttgggtcag 
      ccagtggtg acagtttgg aggatgacg agcggttca 
      caactcctg taactgaat tagatgaaa actcccaac 
      tagttacat ggattgtgc caacaataa ttttggcgc 
      tattggtgg gaacgctag taatacttg gatttcgtt 
      ggtggtgtt gttcgaaga aaggaagaa aagaaacga 
      aaacagccc gatctacca cttacgagc gaataaaat 
      taacgaaac ctacgatgt ttaacaaca actatatcg 
      gcaataata acaaagacg aacagaata atgtgaaaa 
      atattaaag gtagtgtgt gaacagtag ctttaatcg 
      ttatttatc gtttccttt aatatttaa ttgtgataa 
      tttttgcaa caagacttt gttgtgtag atgaaaata 
      actgaagta ttttaagtg ggtatttct gataaaatc 
      atatgcatc tacgtgtag ctctattta ttactagtt 
      tgtattagt gctcattta aaacgattg tttccgttt 
      cattttgtc acagttttg tggatttgt aaaagaact 
      gattattgt atggattat acatgaaat agattgttt 
      cttgccgcc atacattgg aaaaacttg gtagaaaat 
      accacaagt taggtaggt atagagctg aatctgtga 
      ttttataaa aaaaattag gttgtcttt ttccatttt 
      ctctaacat tgcaaaatt ttgttacat cgttttgtt 
      atctattga tttgttaaa tttgcattt aacttatgt 
      gcagctaaa ctgtaattt ccaatggat ggcatctac 
      agttcggtg ttaacggtt ttaaaaata atgatgtac 
      agaatttca attttttaa atctacgac tacagttgg 
      attgttata aaattagaa attttatgt aacataaat 
      ttaagttgt aagtaatat tgttcctat atttaagaa 
      ataagaata tttttctag gtaatttat tttcgtagc 
      aaaatttat ttttcagta ttttgaaag tatttttgt 
      aaagtattt taatattta ctttttatg tatgataga 
      gtcggttat aggaagtgc tgaggatgg aaaaataat 
      gtttcatga tgacaaaat tttaatcgt tagacttta 
      attgtagtt cctttgtta attttagta taactcaat 
      gcaatatgt atatttaat gtggtaggg atagcttga 
      aatgctctt cacacacaa acacaacga attcgtgtg 
      taacacata cgcctaaat gtacctatt taatttttt 
      gttactaga attgctatt aattaggca taactttta 
      cgttaaata atattttat ttataccta tttttaaac 
      catgtaaat tattattat gtataaagt tccaataaa 
      aatctacct ttttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY