Status
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Gene information
General information
TC003818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 21523351..21536326
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003818
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00621

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003818 RA
      MWSSQPVNN GTQNNKENV TQKNDTKIP TLGMTSAIS 
      TAPPKPSDL LKTQELEEA LKPFGVFES EQELNHRMV 
      ILGKLYSLV KQWIKDVSI SKNMPESVA ENVGGKIYT 
      FGSYRLGVH NRGADIDAL CVAPRHISR NDFFGSFYE 
      LLKKQPEVT DLRAVEEAF VPVIKMNFD GIEIDMLFA 
      RLLLKEIPD SMDLRDDLL LKNLDQKCV RSLNGCRVT 
      DEILRLVPN VDNFRLALR AIKLWAKRH GIYSNALGY 
      LGGVSWAML VARTCQLYP NAAPATLVH KFFLVFSQW 
      KWPQPVLLK QPSNVNLGF AVWDPRVNI QDRYHLMPI 
      ITPAYPQQN STFNVSGST RQIIMEEFK LGLQLTDDI 
      MLSKQTWDK LFEPPLFFM KYKHFIVLL VSAESPEDH 
      LEWCGLVES KFRLLIGTL ERNQHITLA HINPESFSL 
      LESQRESNT HCSMWFIGL EFAKSENLN VNLTFDIQQ 
      FTETVQNHA LNISMLKEG MKLEARHVK RKQLYQYLS 
      PSLLKRERK TSITVKSQS NGTDSKKRL SDPGNSDSD 
      NPNKKIRLS EEMHSTYDD SSNNSTSLN CNEDSNSNI 
      SLTEEAHSV SQSAPSHTS STPSTQEAV CT
    CDS sequence
    • >TC003818 RA
      atgtggtca tctcaacct gttaataat ggtactcaa 
      aacaataaa gaaaatgtt acacaaaaa aatgacact 
      aaaatacct accctgggt atgacctca gctatatcc 
      acagctccc cctaaacca tctgatctt ttaaagacc 
      caggaatta gaagaagcg ctaaaacca ttcggtgtt 
      ttcgaaagc gaacaagag ctgaaccat cgaatggtt 
      attttaggt aaattgtac tctttagtt aagcagtgg 
      atcaaagat gtatcaatc tcgaaaaac atgccagaa 
      tctgtggct gaaaatgtg ggcgggaaa atatacacc 
      tttggcagt tatagatta ggggttcat aacagggga 
      gctgacatt gacgcactt tgtgtagca ccaagacat 
      atttctaga aacgatttt tttggttcg ttttacgaa 
      ttgcttaaa aaacaacca gaagtcact gatttaagg 
      gccgttgaa gaagccttt gttccagta attaaaatg 
      aattttgat ggcatagag atagatatg ttgttcgct 
      agattactg cttaaagaa atcccggac tcgatggac 
      ctccgggac gatcttctg ttgaagaac ttggaccag 
      aaatgtgtt cgaagcttg aacggttgc agagtaacc 
      gacgaaatt ctgcgtctc gtccccaat gtagataat 
      tttagatta gcgcttagg gcgattaaa ttatgggcc 
      aaaagacat gggatttat agtaacgct ttgggatat 
      cttggtggc gtttcgtgg gcgatgctc gtggcgcga 
      acttgtcaa ttgtatcca aatgcagct cctgcgact 
      ctagttcat aaatttttt cttgtcttt tctcagtgg 
      aaatggcct cagccagta ttactcaag cagccttca 
      aatgtcaat cttgggttt gccgtgtgg gatccacgg 
      gtaaatatt caagacaga tatcatttg atgccaatc 
      ataacccca gcctaccca caacaaaac tcgacattc 
      aatgtatca ggatcgacg cgacagata ataatggag 
      gaatttaaa ttgggtcta caacttacg gacgatata 
      atgttgagc aaacaaacg tgggataaa cttttcgaa 
      ccaccgtta ttttttatg aagtataag cattttatt 
      gttttatta gttagtgct gaatcgcct gaagatcat 
      ttggaatgg tgtggttta gtggagagt aaattccga 
      ttgcttata ggaacattg gagaggaat cagcacatt 
      acactggca cacataaac ccggaaagt ttcagtctc 
      ttggaatcg caacgcgaa tcaaatacg cattgctcc 
      atgtggttc ataggttta gaatttgcg aaatcagaa 
      aatctaaac gtaaacctt acattcgac attcaacaa 
      ttcaccgaa actgtccaa aatcacgct ttaaacatc 
      agtatgctc aaagagggc atgaaactt gaagcgcgt 
      catgtcaaa agaaaacaa ctctaccag tatctatcg 
      cccagttta ttgaaacgc gaacgcaaa acgagcatc 
      accgtcaaa tcgcagtcc aatggaacg gacagcaaa 
      aagcgcctc tcagacccc ggaaacagc gacagcgat 
      aatcccaat aagaaaatt cgattatct gaggaaatg 
      cattcgacg tacgacgac agttcgaat aattcaaca 
      agcttgaat tgcaacgag gactcaaac agcaacatt 
      agcctaacg gaggaagct catagcgtg tcacaaagc 
      gccccctca cacacatcc agtacgcca tcaacacag 
      gaagctgtc tgtacctga
    mRNA sequence
    • >TC003818 RA
      ctgtcagtc attttgtgc aaaatttgt gatgagtgt 
      cagtgtgat agtgtagaa aacacgtta tcattattt 
      ttataatgt ttttttcac cgatttaaa cccaaatac 
      tataatttg ttactaatt tcttagtgt tagtgttcc 
      tatttaaga tttagtata aagtaagaa tgtggtcat 
      ctcaacctg ttaataatg gtactcaaa acaataaag 
      aaaatgtta cacaaaaaa atgacacta aaataccta 
      ccctgggta tgacctcag ctatatcca cagctcccc 
      ctaaaccat ctgatcttt taaagaccc aggaattag 
      aagaagcgc taaaaccat tcggtgttt tcgaaagcg 
      aacaagagc tgaaccatc gaatggtta ttttaggta 
      aattgtact ctttagtta agcagtgga tcaaagatg 
      tatcaatct cgaaaaaca tgccagaat ctgtggctg 
      aaaatgtgg gcgggaaaa tatacacct ttggcagtt 
      atagattag gggttcata acaggggag ctgacattg 
      acgcacttt gtgtagcac caagacata tttctagaa 
      acgattttt ttggttcgt tttacgaat tgcttaaaa 
      aacaaccag aagtcactg atttaaggg ccgttgaag 
      aagcctttg ttccagtaa ttaaaatga attttgatg 
      gcatagaga tagatatgt tgttcgcta gattactgc 
      ttaaagaaa tcccggact cgatggacc tccgggacg 
      atcttctgt tgaagaact tggaccaga aatgtgttc 
      gaagcttga acggttgca gagtaaccg acgaaattc 
      tgcgtctcg tccccaatg tagataatt ttagattag 
      cgcttaggg cgattaaat tatgggcca aaagacatg 
      ggatttata gtaacgctt tgggatatc ttggtggcg 
      tttcgtggg cgatgctcg tggcgcgaa cttgtcaat 
      tgtatccaa atgcagctc ctgcgactc tagttcata 
      aattttttc ttgtctttt ctcagtgga aatggcctc 
      agccagtat tactcaagc agccttcaa atgtcaatc 
      ttgggtttg ccgtgtggg atccacggg taaatattc 
      aagacagat atcatttga tgccaatca taaccccag 
      cctacccac aacaaaact cgacattca atgtatcag 
      gatcgacgc gacagataa taatggagg aatttaaat 
      tgggtctac aacttacgg acgatataa tgttgagca 
      aacaaacgt gggataaac ttttcgaac caccgttat 
      tttttatga agtataagc attttattg ttttattag 
      ttagtgctg aatcgcctg aagatcatt tggaatggt 
      gtggtttag tggagagta aattccgat tgcttatag 
      gaacattgg agaggaatc agcacatta cactggcac 
      acataaacc cggaaagtt tcagtctct tggaatcgc 
      aacgcgaat caaatacgc attgctcca tgtggttca 
      taggtttag aatttgcga aatcagaaa atctaaacg 
      taaacctta cattcgaca ttcaacaat tcaccgaaa 
      ctgtccaaa atcacgctt taaacatca gtatgctca 
      aagagggca tgaaacttg aagcgcgtc atgtcaaaa 
      gaaaacaac tctaccagt atctatcgc ccagtttat 
      tgaaacgcg aacgcaaaa cgagcatca ccgtcaaat 
      cgcagtcca atggaacgg acagcaaaa agcgcctct 
      cagaccccg gaaacagcg acagcgata atcccaata 
      agaaaattc gattatctg aggaaatgc attcgacgt 
      acgacgaca gttcgaata attcaacaa gcttgaatt 
      gcaacgagg actcaaaca gcaacatta gcctaacgg 
      aggaagctc atagcgtgt cacaaagcg ccccctcac 
      acacatcca gtacgccat caacacagg aagctgtct 
      gtacctgat ccccataac gtgcattag cagcagcca 
      ttgattgtg tacataaaa cgtaagatt ttttcattt 
      ttttcgtca taaatcaat cgaacgaag aagaataaa 
      atgggaatg ttagtttat ttttaggtc gagccacgt 
      actacgtag accactctc gatcacgta cgtataaat 
      agttaacag aaatcaatg atggtaaaa tattatcgc 
      aaacgtgca atgttcgta ttttagctt ttgtgtatg 
      tgtggttag cgtgaatgt gttttgcaa tgtgtctgt 
      aaataaatt gataaataa aaaaaaaca cggaaaaaa 
      atttacttt attcgtagt tggttattt tttgcggta 
      atattttac gctgttttg taacaaatg gtttcgaaa 
      agaatttta agcagtttt tatttgttt tatttttga 
      taacagaag ctcatattt tggcggaac gtattgtat 
      tattttaca ttaatatta attaattgt agaagtgtt 
      tcagtttaa ttattttgt cggtaaaat tattgattc 
      tttaccaaa ttcttttat aaaattagg gggtttgta 
      atgctgttt aaagttctt tcaaggagg atgtcttaa 
      tttaggaat tactgttgc aagtatgaa acccgacat 
      gtaaacaat aaaaaataa agaagcatt tcccct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY