| General information | |||
| TC032563
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 21139433..21170509 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032563 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00619 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 5 (9 dpi)tropharium sometimes shape rounded - Number of animals showing the phenotype: 3
vitellogenic egg chamber number increased - Number of animals showing the phenotype: 3
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
tropharium size decreased - Number of animals showing the phenotype: 3
- >TC032563 RA
MTTLRNTLE VFDTYKHRE EKDSDEESF HLPLDDDES DQSKSFLLN KMNETADSA YDKFDKWCK ENDMHEITE QVVLSYFKL QRNKYKAST VHSIFLMLK DALSLHKGV DISQYSRLQ ALLKSVPRN RKLFALAFD DLKSHSLYE RRYQHFENW RKENKMYDV TEEVVLAYF ELQRNKYKA STLWSNFSI LRQCLSVDK EIDISQYTR LQALLKSAS EGYSPRKSN ALEEEDVWK FLQEADDTW YLAMKVILI MGYYGACRR DELTNMLID DVEFRGESI VVSVPKIKK ITKSVRMFS ITDQFCVNK IKKYVALRP SDVTHKRFF LTYRNGYCV KSPIGVNTM GIIPKEIAA FLKLPNPDR YTGHCFKRT SATHLTNRF NNMLLVKRF DNGNPEVFQ DQSDSTEET SSDLNLPGC SGMCNTNSY FTVSSSNFY KDIIVQEFQ DKKDENETE EEILNKFGS STAPVEHID AQMEEQLLN DEEDEKKVN HHVSKEADK VERVNGFGS EETSSVEEG VKTADAPDS GKNTNSPSQ NLEEQPTSV VDESGAIGT PCSKDSVKE IEADKLAAQ VNGLIDSLE DKEQNCESK NKDPVTKNN DNPPAVDEA KCDNSRNSP KLQNEDSED LFDISIIRD AEEDNAESA YNEAQAEES HEDSDEKAE DEVTNEKAA EEMEEVEEM DIDRQELND VEIEEANLK ETSDGESEN KTETINSEE KLCDNVVVG DKSANECML DEITGKLTN GEGAEMETD KDTEDGQKR RSLKRASSA EDEHSLAKK SRTDPLGDI ESKVEEEPK EKPPLRKLL SLAEFMKTR KNGRKPTMH DLEEFCVQK ICEAIVHKS DLGDVHHQL KVQEQLIET MRKEILQLT KQARDLEIV NKKLMNDLK QHNATQKPL SPLKITRSV GLQVKFTPV SSFEANKRR QVNPQPTQN QKIAPLQPA TNRSPRAAP APVQTNANR QASPQKPQQ QAQVAKPSG ATALLSQAL QQNRRVSDA APKTPPAIR SKPPETKQG GPSASVIDL TDEDDKNKS NKMGAQKNI SVPLRTTPM RVNAQPQGI RLATSQGKV TPMSSVVTS SAPQLMYVV QSAPQNVLN TTLGQKAVV VNFQSANGV ITSTLNGSA VSVIPKQAN TIQLKPVST AKPISSAKV VSPKPIKMS NKHPAPLPQ PPVIRVSCD KSLKPIPPK PHLTIRKTD TGIILQWKM PYDLDMYEV IASYQLYAY QETSATPST DMWRKVGDV KALALPMAC TLTQFADKN KYYFAVRPV DIHKRIGMF SDPEEISL
- >TC032563 RA
atgacaacg ttaagaaac actcttgaa gtttttgac acgtacaag catcgagaa gagaaggat tcagatgaa gaaagtttc cacttacca cttgacgac gatgagtcg gatcaaagt aaatctttt ttacttaac aaaatgaat gagacggcc gatagtgct tatgacaaa tttgacaaa tggtgtaag gagaacgac atgcacgaa ataaccgaa caagtcgtg ctgtcatat tttaaattg caacgaaat aaatacaag gcgtccacc gtgcactcc atctttttg atgttgaag gacgctcta agtttgcac aaaggtgtt gatatttcg cagtacagt agactgcaa gcgctgtta aagtctgtg cccagaaac aggaagctg ttcgctttg gccttcgat gatttaaaa tcgcattcg ctttacgaa agacgctat caacatttt gagaattgg cgtaaggag aacaaaatg tacgacgta accgaagag gttgtgctt gcatatttt gagttacaa cggaataaa tacaaggct tcgactctc tggtccaac ttttcgata ttaaggcag tgcctgagt gtggacaaa gagattgac atttcgcag tacactcgc ctacaggca ttgctaaag agcgcctct gagggctac agtccgaga aaatcaaac gctttggag gaggaagac gtttggaaa tttctacaa gaagccgat gacacgtgg tacttggcc atgaaagtt attttaatt atgggatat tacggggcg tgcagacga gatgagctc actaatatg ttgattgac gatgttgag ttcagaggc gagtcaatt gttgtctca gtgcccaaa atcaaaaaa atcacgaag tcagtacga atgttttcg ataacggat caattctgt gtgaataaa attaagaaa tatgtggcc ttgcggccg tcagatgtc acacataaa cgattcttt ctaacttat cgcaatggc tactgtgtg aaaagtccc attggtgtg aataccatg ggcatcata cccaaagaa attgcagct ttcttaaaa ctacccaat ccagaccgt tacaccggc cattgtttc aaacggaca agtgccact cacttgacc aatcgcttt aataacatg ttactagtc aaacgtttt gacaatggc aaccctgag gtgtttcaa gatcagtca gatagtact gaagaaacg tcttctgat ttgaactta ccagggtgt tctggcatg tgcaacacc aatagttac tttactgtc agtagcagc aatttttat aaagatatc attgtccaa gaatttcaa gacaagaaa gacgaaaac gaaacggaa gaagaaatc ttaaataaa ttcggaagc tcaactgcg ccggttgaa cacattgac gcccaaatg gaagaacag ttgttgaat gatgaagaa gacgagaag aaagtgaat caccacgtt tcgaaggaa gcggacaaa gttgaacgg gttaatggt ttcggaagt gaagaaact tcgtcggtg gaagaaggt gtcaaaaca gcagatgct ccagattct gggaagaat accaatagt ccaagtcag aatctagag gaacaacca accagtgtt gtcgatgaa tctggtgca attggcaca ccctgtagt aaagattca gttaaagaa atcgaagct gataagtta gccgcgcaa gtcaatggt ctaatcgat agtcttgaa gataaagaa caaaactgt gaaagcaaa aacaaagat ccggtgact aaaaataat gataatccg ccagcggtt gacgaagca aaatgtgac aattctcgc aactctcca aagctccaa aatgaggat tctgaagac ttgttcgat attagcatc attcgggat gcggaggaa gacaatgcg gaaagtgca tacaacgaa gcacaagct gaggaaagt catgaagat agtgacgaa aaggctgaa gatgaagtt acgaatgag aaggctgct gaagaaatg gaagaagtg gaagaaatg gatattgac aggcaggag ttgaacgat gtggagatt gaagaggcc aatttgaag gaaactagt gatggggaa agtgaaaat aagactgag acaataaat agtgaagag aaactttgt gataatgtt gtcgtgggg gataaaagt gcgaatgag tgtatgctg gatgaaatc acggggaag ttaacgaat ggggaaggg gcagaaatg gaaacggat aaagatact gaggatggt cagaaacgg cgatcgttg aaaagagcc agttccgct gaggatgag cacagttta gcgaaaaaa tcacgaaca gatccttta ggcgacatc gagtcgaaa gtagaagaa gaaccaaaa gaaaaaccg ccattacga aaactactc tcgttagcc gaatttatg aaaactaga aaaaatggt agaaaaccg acaatgcac gatttagaa gaattttgc gtccaaaaa atctgcgaa gcgatagtc cataaaagc gatctaggc gacgtccac catcaacta aaagtacaa gaacaatta atcgagaca atgcgcaag gaaatccta cagcttacg aaacaagcg cgcgatttg gaaatcgta aataaaaaa ttaatgaac gatttaaaa cagcacaat gccacacaa aaacccttg agtccattg aaaatcact cgttcggtg ggattgcaa gtcaaattc accccagtt tcgagcttt gaagccaac aaacgaaga caggtgaat ccgcagccg actcaaaac caaaaaatt gctccactc caacctgca acgaataga agcccacga gctgccccc gcccccgtt caaactaac gcaaatcga caagcgagt cctcagaaa ccccagcag caagctcaa gtggccaaa cctagcggc gccactgcc ttgctttcc caagctttg caacagaat cgaagggtg agcgacgct gcccccaag acacctcct gcaattcga agcaaacct cccgaaacg aaacaggga ggaccttca gcttctgtc atcgattta accgatgaa gatgacaaa aataaatcg aacaaaatg ggggcccaa aagaacatt tcagttcct ttgaggaca acgcccatg cgggtgaat gcacaacca caagggatc aggttggcg acttcgcaa ggaaaggtg actccgatg agttcagtg gtgacctca agcgccccc caactcatg tatgtcgtg cagtcggcg ccccagaat gttttgaat acgacgctg gggcagaag gccgttgtt gtgaatttc cagtctgct aatggtgtt ataacgtct accttgaac gggtccgca gtgtccgtc attccaaag caggcgaat acaattcag ctgaagccc gtgtcaact gcaaagcct atcagtagt gcaaaagtg gtgtcgcct aagcccatt aaaatgtca aacaaacac ccagcccca ttgccacaa ccgccggtg attcgagtg agctgcgat aagtcactc aagccaata ccaccaaag ccgcattta accattcga aaaactgat actgggatc attcttcaa tggaagatg ccgtacgac ctggacatg tacgaagtc atagctagt tatcagttg tatgcatat caagagact agtgcgacg cctagtacg gacatgtgg cgcaaagtg ggtgatgtg aaggcgcta gcgcttccg atggcgtgc actttgacg cagtttgcc gataaaaat aaatactat ttcgctgta cgtcctgtg gatattcat aaaagaatc ggaatgttt agtgatccg gaagagata tcgttgtaa
- >TC032563 RA
tgatgtgac cgacaattg tatgactcc acgtcacca agtgctatc gattaagat tgacccact aatcctaca gttgggcct tgtcataac gcccatatg ggtcaatgc ccactggat attgcaata caaaaccac aaaatagta tttttctcc ttttaattg aaaaccaaa gttggcgtc aagtcactg gatatagcc gttgtcgag tacaaaagt aagcgcggg tttgttgcg ccgtggcac gaccatctg gcaacagcg ctcagtcca cagtctttc ccgacgacg ctttttatc atctagtgg gcacggtga ataaccaag aattgtggc ggcattatt gttgtgttt tcttaaaca aattaggtt aagtttcga cgatttgac acattatat taatgctgt caagaattt tattttaag ctatgatgc catccttgg atcactgta taaataacc cacccacaa tgacaacgt taagaaaca ctcttgaag tttttgaca cgtacaagc atcgagaag agaaggatt cagatgaag aaagtttcc acttaccac ttgacgacg atgagtcgg atcaaagta aatcttttt tacttaaca aaatgaatg agacggccg atagtgctt atgacaaat ttgacaaat ggtgtaagg agaacgaca tgcacgaaa taaccgaac aagtcgtgc tgtcatatt ttaaattgc aacgaaata aatacaagg cgtccaccg tgcactcca tctttttga tgttgaagg acgctctaa gtttgcaca aaggtgttg atatttcgc agtacagta gactgcaag cgctgttaa agtctgtgc ccagaaaca ggaagctgt tcgctttgg ccttcgatg atttaaaat cgcattcgc tttacgaaa gacgctatc aacattttg agaattggc gtaaggaga acaaaatgt acgacgtaa ccgaagagg ttgtgcttg catattttg agttacaac ggaataaat acaaggctt cgactctct ggtccaact tttcgatat taaggcagt gcctgagtg tggacaaag agattgaca tttcgcagt acactcgcc tacaggcat tgctaaaga gcgcctctg agggctaca gtccgagaa aatcaaacg ctttggagg aggaagacg tttggaaat ttctacaag aagccgatg acacgtggt acttggcca tgaaagtta ttttaatta tgggatatt acggggcgt gcagacgag atgagctca ctaatatgt tgattgacg atgttgagt tcagaggcg agtcaattg ttgtctcag tgcccaaaa tcaaaaaaa tcacgaagt cagtacgaa tgttttcga taacggatc aattctgtg tgaataaaa ttaagaaat atgtggcct tgcggccgt cagatgtca cacataaac gattctttc taacttatc gcaatggct actgtgtga aaagtccca ttggtgtga ataccatgg gcatcatac ccaaagaaa ttgcagctt tcttaaaac tacccaatc cagaccgtt acaccggcc attgtttca aacggacaa gtgccactc acttgacca atcgcttta ataacatgt tactagtca aacgttttg acaatggca accctgagg tgtttcaag atcagtcag atagtactg aagaaacgt cttctgatt tgaacttac cagggtgtt ctggcatgt gcaacacca atagttact ttactgtca gtagcagca atttttata aagatatca ttgtccaag aatttcaag acaagaaag acgaaaacg aaacggaag aagaaatct taaataaat tcggaagct caactgcgc cggttgaac acattgacg cccaaatgg aagaacagt tgttgaatg atgaagaag acgagaaga aagtgaatc accacgttt cgaaggaag cggacaaag ttgaacggg ttaatggtt tcggaagtg aagaaactt cgtcggtgg aagaaggtg tcaaaacag cagatgctc cagattctg ggaagaata ccaatagtc caagtcaga atctagagg aacaaccaa ccagtgttg tcgatgaat ctggtgcaa ttggcacac cctgtagta aagattcag ttaaagaaa tcgaagctg ataagttag ccgcgcaag tcaatggtc taatcgata gtcttgaag ataaagaac aaaactgtg aaagcaaaa acaaagatc cggtgacta aaaataatg ataatccgc cagcggttg acgaagcaa aatgtgaca attctcgca actctccaa agctccaaa atgaggatt ctgaagact tgttcgata ttagcatca ttcgggatg cggaggaag acaatgcgg aaagtgcat acaacgaag cacaagctg aggaaagtc atgaagata gtgacgaaa aggctgaag atgaagtta cgaatgaga aggctgctg aagaaatgg aagaagtgg aagaaatgg atattgaca ggcaggagt tgaacgatg tggagattg aagaggcca atttgaagg aaactagtg atggggaaa gtgaaaata agactgaga caataaata gtgaagaga aactttgtg ataatgttg tcgtggggg ataaaagtg cgaatgagt gtatgctgg atgaaatca cggggaagt taacgaatg gggaagggg cagaaatgg aaacggata aagatactg aggatggtc agaaacggc gatcgttga aaagagcca gttccgctg aggatgagc acagtttag cgaaaaaat cacgaacag atcctttag gcgacatcg agtcgaaag tagaagaag aaccaaaag aaaaaccgc cattacgaa aactactct cgttagccg aatttatga aaactagaa aaaatggta gaaaaccga caatgcacg atttagaag aattttgcg tccaaaaaa tctgcgaag cgatagtcc ataaaagcg atctaggcg acgtccacc atcaactaa aagtacaag aacaattaa tcgagacaa tgcgcaagg aaatcctac agcttacga aacaagcgc gcgatttgg aaatcgtaa ataaaaaat taatgaacg atttaaaac agcacaatg ccacacaaa aacccttga gtccattga aaatcactc gttcggtgg gattgcaag tcaaattca ccccagttt cgagctttg aagccaaca aacgaagac aggtgaatc cgcagccga ctcaaaacc aaaaaattg ctccactcc aacctgcaa cgaatagaa gcccacgag ctgcccccg cccccgttc aaactaacg caaatcgac aagcgagtc ctcagaaac cccagcagc aagctcaag tggccaaac ctagcggcg ccactgcct tgctttccc aagctttgc aacagaatc gaagggtga gcgacgctg cccccaaga cacctcctg caattcgaa gcaaacctc ccgaaacga aacagggag gaccttcag cttctgtca tcgatttaa ccgatgaag atgacaaaa ataaatcga acaaaatgg gggcccaaa agaacattt cagttcctt tgaggacaa cgcccatgc gggtgaatg cacaaccac aagggatca ggttggcga cttcgcaag gaaaggtga ctccgatga gttcagtgg tgacctcaa gcgcccccc aactcatgt atgtcgtgc agtcggcgc cccagaatg ttttgaata cgacgctgg ggcagaagg ccgttgttg tgaatttcc agtctgcta atggtgtta taacgtcta ccttgaacg ggtccgcag tgtccgtca ttccaaagc aggcgaata caattcagc tgaagcccg tgtcaactg caaagccta tcagtagtg caaaagtgg tgtcgccta agcccatta aaatgtcaa acaaacacc cagccccat tgccacaac cgccggtga ttcgagtga gctgcgata agtcactca agccaatac caccaaagc cgcatttaa ccattcgaa aaactgata ctgggatca ttcttcaat ggaagatgc cgtacgacc tggacatgt acgaagtca tagctagtt atcagttgt atgcatatc aagagacta gtgcgacgc ctagtacgg acatgtggc gcaaagtgg gtgatgtga aggcgctag cgcttccga tggcgtgca ctttgacgc agtttgccg ataaaaata aatactatt tcgctgtac gtcctgtgg atattcata aaagaatcg gaatgttta gtgatccgg aagagatat cgttgtaaa tatttatta ttgtaataa ctatgtata ttataagca ttcattcgt gtttatttt tgttttcta atatttttg tttgtaata tgcattaaa ttaagaact agagg
