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Gene information
General information
TC032563
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003814
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 21139433..21170509
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032563
    iBeetle screen iB_00619

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 3
    tropharium sometimes shape rounded - Number of animals showing the phenotype: 3

    vitellogenic egg chamber number increased - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    tropharium size decreased - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032563 RA
      MTTLRNTLE VFDTYKHRE EKDSDEESF HLPLDDDES 
      DQSKSFLLN KMNETADSA YDKFDKWCK ENDMHEITE 
      QVVLSYFKL QRNKYKAST VHSIFLMLK DALSLHKGV 
      DISQYSRLQ ALLKSVPRN RKLFALAFD DLKSHSLYE 
      RRYQHFENW RKENKMYDV TEEVVLAYF ELQRNKYKA 
      STLWSNFSI LRQCLSVDK EIDISQYTR LQALLKSAS 
      EGYSPRKSN ALEEEDVWK FLQEADDTW YLAMKVILI 
      MGYYGACRR DELTNMLID DVEFRGESI VVSVPKIKK 
      ITKSVRMFS ITDQFCVNK IKKYVALRP SDVTHKRFF 
      LTYRNGYCV KSPIGVNTM GIIPKEIAA FLKLPNPDR 
      YTGHCFKRT SATHLTNRF NNMLLVKRF DNGNPEVFQ 
      DQSDSTEET SSDLNLPGC SGMCNTNSY FTVSSSNFY 
      KDIIVQEFQ DKKDENETE EEILNKFGS STAPVEHID 
      AQMEEQLLN DEEDEKKVN HHVSKEADK VERVNGFGS 
      EETSSVEEG VKTADAPDS GKNTNSPSQ NLEEQPTSV 
      VDESGAIGT PCSKDSVKE IEADKLAAQ VNGLIDSLE 
      DKEQNCESK NKDPVTKNN DNPPAVDEA KCDNSRNSP 
      KLQNEDSED LFDISIIRD AEEDNAESA YNEAQAEES 
      HEDSDEKAE DEVTNEKAA EEMEEVEEM DIDRQELND 
      VEIEEANLK ETSDGESEN KTETINSEE KLCDNVVVG 
      DKSANECML DEITGKLTN GEGAEMETD KDTEDGQKR 
      RSLKRASSA EDEHSLAKK SRTDPLGDI ESKVEEEPK 
      EKPPLRKLL SLAEFMKTR KNGRKPTMH DLEEFCVQK 
      ICEAIVHKS DLGDVHHQL KVQEQLIET MRKEILQLT 
      KQARDLEIV NKKLMNDLK QHNATQKPL SPLKITRSV 
      GLQVKFTPV SSFEANKRR QVNPQPTQN QKIAPLQPA 
      TNRSPRAAP APVQTNANR QASPQKPQQ QAQVAKPSG 
      ATALLSQAL QQNRRVSDA APKTPPAIR SKPPETKQG 
      GPSASVIDL TDEDDKNKS NKMGAQKNI SVPLRTTPM 
      RVNAQPQGI RLATSQGKV TPMSSVVTS SAPQLMYVV 
      QSAPQNVLN TTLGQKAVV VNFQSANGV ITSTLNGSA 
      VSVIPKQAN TIQLKPVST AKPISSAKV VSPKPIKMS 
      NKHPAPLPQ PPVIRVSCD KSLKPIPPK PHLTIRKTD 
      TGIILQWKM PYDLDMYEV IASYQLYAY QETSATPST 
      DMWRKVGDV KALALPMAC TLTQFADKN KYYFAVRPV 
      DIHKRIGMF SDPEEISL
    CDS sequence
    • >TC032563 RA
      atgacaacg ttaagaaac actcttgaa gtttttgac 
      acgtacaag catcgagaa gagaaggat tcagatgaa 
      gaaagtttc cacttacca cttgacgac gatgagtcg 
      gatcaaagt aaatctttt ttacttaac aaaatgaat 
      gagacggcc gatagtgct tatgacaaa tttgacaaa 
      tggtgtaag gagaacgac atgcacgaa ataaccgaa 
      caagtcgtg ctgtcatat tttaaattg caacgaaat 
      aaatacaag gcgtccacc gtgcactcc atctttttg 
      atgttgaag gacgctcta agtttgcac aaaggtgtt 
      gatatttcg cagtacagt agactgcaa gcgctgtta 
      aagtctgtg cccagaaac aggaagctg ttcgctttg 
      gccttcgat gatttaaaa tcgcattcg ctttacgaa 
      agacgctat caacatttt gagaattgg cgtaaggag 
      aacaaaatg tacgacgta accgaagag gttgtgctt 
      gcatatttt gagttacaa cggaataaa tacaaggct 
      tcgactctc tggtccaac ttttcgata ttaaggcag 
      tgcctgagt gtggacaaa gagattgac atttcgcag 
      tacactcgc ctacaggca ttgctaaag agcgcctct 
      gagggctac agtccgaga aaatcaaac gctttggag 
      gaggaagac gtttggaaa tttctacaa gaagccgat 
      gacacgtgg tacttggcc atgaaagtt attttaatt 
      atgggatat tacggggcg tgcagacga gatgagctc 
      actaatatg ttgattgac gatgttgag ttcagaggc 
      gagtcaatt gttgtctca gtgcccaaa atcaaaaaa 
      atcacgaag tcagtacga atgttttcg ataacggat 
      caattctgt gtgaataaa attaagaaa tatgtggcc 
      ttgcggccg tcagatgtc acacataaa cgattcttt 
      ctaacttat cgcaatggc tactgtgtg aaaagtccc 
      attggtgtg aataccatg ggcatcata cccaaagaa 
      attgcagct ttcttaaaa ctacccaat ccagaccgt 
      tacaccggc cattgtttc aaacggaca agtgccact 
      cacttgacc aatcgcttt aataacatg ttactagtc 
      aaacgtttt gacaatggc aaccctgag gtgtttcaa 
      gatcagtca gatagtact gaagaaacg tcttctgat 
      ttgaactta ccagggtgt tctggcatg tgcaacacc 
      aatagttac tttactgtc agtagcagc aatttttat 
      aaagatatc attgtccaa gaatttcaa gacaagaaa 
      gacgaaaac gaaacggaa gaagaaatc ttaaataaa 
      ttcggaagc tcaactgcg ccggttgaa cacattgac 
      gcccaaatg gaagaacag ttgttgaat gatgaagaa 
      gacgagaag aaagtgaat caccacgtt tcgaaggaa 
      gcggacaaa gttgaacgg gttaatggt ttcggaagt 
      gaagaaact tcgtcggtg gaagaaggt gtcaaaaca 
      gcagatgct ccagattct gggaagaat accaatagt 
      ccaagtcag aatctagag gaacaacca accagtgtt 
      gtcgatgaa tctggtgca attggcaca ccctgtagt 
      aaagattca gttaaagaa atcgaagct gataagtta 
      gccgcgcaa gtcaatggt ctaatcgat agtcttgaa 
      gataaagaa caaaactgt gaaagcaaa aacaaagat 
      ccggtgact aaaaataat gataatccg ccagcggtt 
      gacgaagca aaatgtgac aattctcgc aactctcca 
      aagctccaa aatgaggat tctgaagac ttgttcgat 
      attagcatc attcgggat gcggaggaa gacaatgcg 
      gaaagtgca tacaacgaa gcacaagct gaggaaagt 
      catgaagat agtgacgaa aaggctgaa gatgaagtt 
      acgaatgag aaggctgct gaagaaatg gaagaagtg 
      gaagaaatg gatattgac aggcaggag ttgaacgat 
      gtggagatt gaagaggcc aatttgaag gaaactagt 
      gatggggaa agtgaaaat aagactgag acaataaat 
      agtgaagag aaactttgt gataatgtt gtcgtgggg 
      gataaaagt gcgaatgag tgtatgctg gatgaaatc 
      acggggaag ttaacgaat ggggaaggg gcagaaatg 
      gaaacggat aaagatact gaggatggt cagaaacgg 
      cgatcgttg aaaagagcc agttccgct gaggatgag 
      cacagttta gcgaaaaaa tcacgaaca gatccttta 
      ggcgacatc gagtcgaaa gtagaagaa gaaccaaaa 
      gaaaaaccg ccattacga aaactactc tcgttagcc 
      gaatttatg aaaactaga aaaaatggt agaaaaccg 
      acaatgcac gatttagaa gaattttgc gtccaaaaa 
      atctgcgaa gcgatagtc cataaaagc gatctaggc 
      gacgtccac catcaacta aaagtacaa gaacaatta 
      atcgagaca atgcgcaag gaaatccta cagcttacg 
      aaacaagcg cgcgatttg gaaatcgta aataaaaaa 
      ttaatgaac gatttaaaa cagcacaat gccacacaa 
      aaacccttg agtccattg aaaatcact cgttcggtg 
      ggattgcaa gtcaaattc accccagtt tcgagcttt 
      gaagccaac aaacgaaga caggtgaat ccgcagccg 
      actcaaaac caaaaaatt gctccactc caacctgca 
      acgaataga agcccacga gctgccccc gcccccgtt 
      caaactaac gcaaatcga caagcgagt cctcagaaa 
      ccccagcag caagctcaa gtggccaaa cctagcggc 
      gccactgcc ttgctttcc caagctttg caacagaat 
      cgaagggtg agcgacgct gcccccaag acacctcct 
      gcaattcga agcaaacct cccgaaacg aaacaggga 
      ggaccttca gcttctgtc atcgattta accgatgaa 
      gatgacaaa aataaatcg aacaaaatg ggggcccaa 
      aagaacatt tcagttcct ttgaggaca acgcccatg 
      cgggtgaat gcacaacca caagggatc aggttggcg 
      acttcgcaa ggaaaggtg actccgatg agttcagtg 
      gtgacctca agcgccccc caactcatg tatgtcgtg 
      cagtcggcg ccccagaat gttttgaat acgacgctg 
      gggcagaag gccgttgtt gtgaatttc cagtctgct 
      aatggtgtt ataacgtct accttgaac gggtccgca 
      gtgtccgtc attccaaag caggcgaat acaattcag 
      ctgaagccc gtgtcaact gcaaagcct atcagtagt 
      gcaaaagtg gtgtcgcct aagcccatt aaaatgtca 
      aacaaacac ccagcccca ttgccacaa ccgccggtg 
      attcgagtg agctgcgat aagtcactc aagccaata 
      ccaccaaag ccgcattta accattcga aaaactgat 
      actgggatc attcttcaa tggaagatg ccgtacgac 
      ctggacatg tacgaagtc atagctagt tatcagttg 
      tatgcatat caagagact agtgcgacg cctagtacg 
      gacatgtgg cgcaaagtg ggtgatgtg aaggcgcta 
      gcgcttccg atggcgtgc actttgacg cagtttgcc 
      gataaaaat aaatactat ttcgctgta cgtcctgtg 
      gatattcat aaaagaatc ggaatgttt agtgatccg 
      gaagagata tcgttgtaa
    mRNA sequence
    • >TC032563 RA
      tgatgtgac cgacaattg tatgactcc acgtcacca 
      agtgctatc gattaagat tgacccact aatcctaca 
      gttgggcct tgtcataac gcccatatg ggtcaatgc 
      ccactggat attgcaata caaaaccac aaaatagta 
      tttttctcc ttttaattg aaaaccaaa gttggcgtc 
      aagtcactg gatatagcc gttgtcgag tacaaaagt 
      aagcgcggg tttgttgcg ccgtggcac gaccatctg 
      gcaacagcg ctcagtcca cagtctttc ccgacgacg 
      ctttttatc atctagtgg gcacggtga ataaccaag 
      aattgtggc ggcattatt gttgtgttt tcttaaaca 
      aattaggtt aagtttcga cgatttgac acattatat 
      taatgctgt caagaattt tattttaag ctatgatgc 
      catccttgg atcactgta taaataacc cacccacaa 
      tgacaacgt taagaaaca ctcttgaag tttttgaca 
      cgtacaagc atcgagaag agaaggatt cagatgaag 
      aaagtttcc acttaccac ttgacgacg atgagtcgg 
      atcaaagta aatcttttt tacttaaca aaatgaatg 
      agacggccg atagtgctt atgacaaat ttgacaaat 
      ggtgtaagg agaacgaca tgcacgaaa taaccgaac 
      aagtcgtgc tgtcatatt ttaaattgc aacgaaata 
      aatacaagg cgtccaccg tgcactcca tctttttga 
      tgttgaagg acgctctaa gtttgcaca aaggtgttg 
      atatttcgc agtacagta gactgcaag cgctgttaa 
      agtctgtgc ccagaaaca ggaagctgt tcgctttgg 
      ccttcgatg atttaaaat cgcattcgc tttacgaaa 
      gacgctatc aacattttg agaattggc gtaaggaga 
      acaaaatgt acgacgtaa ccgaagagg ttgtgcttg 
      catattttg agttacaac ggaataaat acaaggctt 
      cgactctct ggtccaact tttcgatat taaggcagt 
      gcctgagtg tggacaaag agattgaca tttcgcagt 
      acactcgcc tacaggcat tgctaaaga gcgcctctg 
      agggctaca gtccgagaa aatcaaacg ctttggagg 
      aggaagacg tttggaaat ttctacaag aagccgatg 
      acacgtggt acttggcca tgaaagtta ttttaatta 
      tgggatatt acggggcgt gcagacgag atgagctca 
      ctaatatgt tgattgacg atgttgagt tcagaggcg 
      agtcaattg ttgtctcag tgcccaaaa tcaaaaaaa 
      tcacgaagt cagtacgaa tgttttcga taacggatc 
      aattctgtg tgaataaaa ttaagaaat atgtggcct 
      tgcggccgt cagatgtca cacataaac gattctttc 
      taacttatc gcaatggct actgtgtga aaagtccca 
      ttggtgtga ataccatgg gcatcatac ccaaagaaa 
      ttgcagctt tcttaaaac tacccaatc cagaccgtt 
      acaccggcc attgtttca aacggacaa gtgccactc 
      acttgacca atcgcttta ataacatgt tactagtca 
      aacgttttg acaatggca accctgagg tgtttcaag 
      atcagtcag atagtactg aagaaacgt cttctgatt 
      tgaacttac cagggtgtt ctggcatgt gcaacacca 
      atagttact ttactgtca gtagcagca atttttata 
      aagatatca ttgtccaag aatttcaag acaagaaag 
      acgaaaacg aaacggaag aagaaatct taaataaat 
      tcggaagct caactgcgc cggttgaac acattgacg 
      cccaaatgg aagaacagt tgttgaatg atgaagaag 
      acgagaaga aagtgaatc accacgttt cgaaggaag 
      cggacaaag ttgaacggg ttaatggtt tcggaagtg 
      aagaaactt cgtcggtgg aagaaggtg tcaaaacag 
      cagatgctc cagattctg ggaagaata ccaatagtc 
      caagtcaga atctagagg aacaaccaa ccagtgttg 
      tcgatgaat ctggtgcaa ttggcacac cctgtagta 
      aagattcag ttaaagaaa tcgaagctg ataagttag 
      ccgcgcaag tcaatggtc taatcgata gtcttgaag 
      ataaagaac aaaactgtg aaagcaaaa acaaagatc 
      cggtgacta aaaataatg ataatccgc cagcggttg 
      acgaagcaa aatgtgaca attctcgca actctccaa 
      agctccaaa atgaggatt ctgaagact tgttcgata 
      ttagcatca ttcgggatg cggaggaag acaatgcgg 
      aaagtgcat acaacgaag cacaagctg aggaaagtc 
      atgaagata gtgacgaaa aggctgaag atgaagtta 
      cgaatgaga aggctgctg aagaaatgg aagaagtgg 
      aagaaatgg atattgaca ggcaggagt tgaacgatg 
      tggagattg aagaggcca atttgaagg aaactagtg 
      atggggaaa gtgaaaata agactgaga caataaata 
      gtgaagaga aactttgtg ataatgttg tcgtggggg 
      ataaaagtg cgaatgagt gtatgctgg atgaaatca 
      cggggaagt taacgaatg gggaagggg cagaaatgg 
      aaacggata aagatactg aggatggtc agaaacggc 
      gatcgttga aaagagcca gttccgctg aggatgagc 
      acagtttag cgaaaaaat cacgaacag atcctttag 
      gcgacatcg agtcgaaag tagaagaag aaccaaaag 
      aaaaaccgc cattacgaa aactactct cgttagccg 
      aatttatga aaactagaa aaaatggta gaaaaccga 
      caatgcacg atttagaag aattttgcg tccaaaaaa 
      tctgcgaag cgatagtcc ataaaagcg atctaggcg 
      acgtccacc atcaactaa aagtacaag aacaattaa 
      tcgagacaa tgcgcaagg aaatcctac agcttacga 
      aacaagcgc gcgatttgg aaatcgtaa ataaaaaat 
      taatgaacg atttaaaac agcacaatg ccacacaaa 
      aacccttga gtccattga aaatcactc gttcggtgg 
      gattgcaag tcaaattca ccccagttt cgagctttg 
      aagccaaca aacgaagac aggtgaatc cgcagccga 
      ctcaaaacc aaaaaattg ctccactcc aacctgcaa 
      cgaatagaa gcccacgag ctgcccccg cccccgttc 
      aaactaacg caaatcgac aagcgagtc ctcagaaac 
      cccagcagc aagctcaag tggccaaac ctagcggcg 
      ccactgcct tgctttccc aagctttgc aacagaatc 
      gaagggtga gcgacgctg cccccaaga cacctcctg 
      caattcgaa gcaaacctc ccgaaacga aacagggag 
      gaccttcag cttctgtca tcgatttaa ccgatgaag 
      atgacaaaa ataaatcga acaaaatgg gggcccaaa 
      agaacattt cagttcctt tgaggacaa cgcccatgc 
      gggtgaatg cacaaccac aagggatca ggttggcga 
      cttcgcaag gaaaggtga ctccgatga gttcagtgg 
      tgacctcaa gcgcccccc aactcatgt atgtcgtgc 
      agtcggcgc cccagaatg ttttgaata cgacgctgg 
      ggcagaagg ccgttgttg tgaatttcc agtctgcta 
      atggtgtta taacgtcta ccttgaacg ggtccgcag 
      tgtccgtca ttccaaagc aggcgaata caattcagc 
      tgaagcccg tgtcaactg caaagccta tcagtagtg 
      caaaagtgg tgtcgccta agcccatta aaatgtcaa 
      acaaacacc cagccccat tgccacaac cgccggtga 
      ttcgagtga gctgcgata agtcactca agccaatac 
      caccaaagc cgcatttaa ccattcgaa aaactgata 
      ctgggatca ttcttcaat ggaagatgc cgtacgacc 
      tggacatgt acgaagtca tagctagtt atcagttgt 
      atgcatatc aagagacta gtgcgacgc ctagtacgg 
      acatgtggc gcaaagtgg gtgatgtga aggcgctag 
      cgcttccga tggcgtgca ctttgacgc agtttgccg 
      ataaaaata aatactatt tcgctgtac gtcctgtgg 
      atattcata aaagaatcg gaatgttta gtgatccgg 
      aagagatat cgttgtaaa tatttatta ttgtaataa 
      ctatgtata ttataagca ttcattcgt gtttatttt 
      tgttttcta atatttttg tttgtaata tgcattaaa 
      ttaagaact agagg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY