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Gene information
General information
TC003806 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 20779637..20791181
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003806
    iBeetle screen iB_00618

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection



    remarks: animals lost,
    Pupal morphology
    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003806 RA
      MDYNLFLFI LVVISSCCC VKCDNIIND FLSVPTTVK 
      AQENNTVLL PCYLNTASN DGNFITAIR WYKDENLVV 
      DSADEQLPL PEGHILWEN GSLEIFRVT PNDTGIYIC 
      EIVRPEPWS PVRQRHAIE VLHPPSVEP FPPTGFLQV 
      KLGEEVRMS CKGSGVPYP IITWYTKGE EIKLLNPRE 
      LLKFTASDR QMAGIYECR AANGVGEPA SAQIELNII 
      YPPELMTSR SWIHTAPGH RVQLECKIS ADPQATVTW 
      TKGDMPVPL DSRVLSLVD GDKYTLLIK NVQKSDFGI 
      YTCKAINEL GQGELQIQL SGVPNPGVF KKTDDKNIN 
      SKDSYTLIW EVDSYTPII EYNLWFQPY KPKTGLSKP 
      NWTKLTIPT EHSSGPVYS KSYTIKGLK EKTVYEALL 
      VSRNRYGWS KPSPILRFA TAGADLKEE MITPVQHHT 
      EDNIIPLDL SSSSQNCKL GNVGFMVLV IVTMYYHFH 
      C
    CDS sequence
    • >TC003806 RA
      atggattat aatttattt ttatttata ttagttgtg 
      attagttcc tgctgttgt gttaagtgc gataatatt 
      ataaatgat tttctaagt gtacccacc actgtgaag 
      gcgcaagaa aataacacc gtcttgtta ccctgttat 
      ttaaatacc gcatcaaat gacggcaat ttcattact 
      gcaataaga tggtacaaa gatgaaaat ttagtagtc 
      gacagtgca gatgaacaa ttaccactt cccgaaggc 
      cacatctta tgggaaaac ggttctttg gaaatcttt 
      agagtaact ccaaacgac actggaatt tatatttgc 
      gaaattgtc agacctgaa ccctggtca cccgtccga 
      caaagacat gcaatcgaa gtcttgcat ccaccgtcg 
      gtggaacct ttccctccg accggcttc ctccaagtg 
      aaactcgga gaagaagtt agaatgtca tgtaaagga 
      agtggggtt ccttacccc atcatcacc tggtacaca 
      aaaggggaa gaaataaag cttctaaat ccgcgagag 
      ttgttaaaa ttcacagcg tccgatcga caaatggcc 
      ggaatttac gaatgtcgt gccgccaat ggagtcggt 
      gaaccagcc tctgcccaa atcgagctc aatataata 
      taccctcca gaactgatg acttcccgc tcttggatt 
      cacactgct cctggtcat agagtccaa ctagaatgc 
      aaaatttcg gctgatcct caagctaca gtgacctgg 
      actaaaggc gacatgccg gtgccttta gacagcaga 
      gtgctctcg ctagtagat ggtgataaa tacactttg 
      ctcatcaaa aacgtccaa aaaagcgat ttcgggatt 
      tacacctgc aaagctatc aacgaattg ggtcaaggg 
      gaactccag atacagctt tcaggtgtg ccaaatcct 
      ggagtgttc aaaaaaaca gatgacaaa aacataaac 
      tcaaaagat tcctatact ctgatctgg gaagttgac 
      agttacaca ccaattata gaatacaat ctatggttc 
      caaccttac aaacccaaa actgggtta agtaaaccc 
      aactggacc aaattaact ataccgaca gaacacagt 
      tcaggacca gtttattca aaatcgtac actatcaaa 
      ggtttgaaa gaaaaaacc gtgtatgag gctttactg 
      gtttcaagg aataggtat gggtggtca aaaccgtca 
      cctattttg agatttgct acagctggc gcggatttg 
      aaagaagag atgattact ccagtgcaa caccataca 
      gaagataat ataattccc ctagatttg agcagttcc 
      tcgcaaaat tgtaaatta gggaatgtt ggttttatg 
      gttttggta attgtgaca atgtattat cattttcat 
      tgttaa
    mRNA sequence
    • >TC003806 RA
      ttcgtcact tagatgcgc tttattgat gtagttcct 
      cttttttta caacacatg tagcatacg agcacccac 
      actcaccgc tccagaatg gattataat ttattttta 
      tttatatta gttgtgatt agttcctgc tgttgtgtt 
      aagtgcgat aatattata aatgatttt ctaagtgta 
      cccaccact gtgaaggcg caagaaaat aacaccgtc 
      ttgttaccc tgttattta aataccgca tcaaatgac 
      ggcaatttc attactgca ataagatgg tacaaagat 
      gaaaattta gtagtcgac agtgcagat gaacaatta 
      ccacttccc gaaggccac atcttatgg gaaaacggt 
      tctttggaa atctttaga gtaactcca aacgacact 
      ggaatttat atttgcgaa attgtcaga cctgaaccc 
      tggtcaccc gtccgacaa agacatgca atcgaagtc 
      ttgcatcca ccgtcggtg gaacctttc cctccgacc 
      ggcttcctc caagtgaaa ctcggagaa gaagttaga 
      atgtcatgt aaaggaagt ggggttcct taccccatc 
      atcacctgg tacacaaaa ggggaagaa ataaagctt 
      ctaaatccg cgagagttg ttaaaattc acagcgtcc 
      gatcgacaa atggccgga atttacgaa tgtcgtgcc 
      gccaatgga gtcggtgaa ccagcctct gcccaaatc 
      gagctcaat ataatatac cctccagaa ctgatgact 
      tcccgctct tggattcac actgctcct ggtcataga 
      gtccaacta gaatgcaaa atttcggct gatcctcaa 
      gctacagtg acctggact aaaggcgac atgccggtg 
      cctttagac agcagagtg ctctcgcta gtagatggt 
      gataaatac actttgctc atcaaaaac gtccaaaaa 
      agcgatttc gggatttac acctgcaaa gctatcaac 
      gaattgggt caaggggaa ctccagata cagctttca 
      ggtgtgcca aatcctgga gtgttcaaa aaaacagat 
      gacaaaaac ataaactca aaagattcc tatactctg 
      atctgggaa gttgacagt tacacacca attatagaa 
      tacaatcta tggttccaa ccttacaaa cccaaaact 
      gggttaagt aaacccaac tggaccaaa ttaactata 
      ccgacagaa cacagttca ggaccagtt tattcaaaa 
      tcgtacact atcaaaggt ttgaaagaa aaaaccgtg 
      tatgaggct ttactggtt tcaaggaat aggtatggg 
      tggtcaaaa ccgtcacct attttgaga tttgctaca 
      gctggcgcg gatttgaaa gaagagatg attactcca 
      gtgcaacac catacagaa gataatata attccccta 
      gatttgagc agttcctcg caaaattgt aaattaggg 
      aatgttggt tttatggtt ttggtaatt gtgacaatg 
      tattatcat tttcattgt taaaaataa acttataag 
      aaagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY