Status
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Gene information
General information
TC003800 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 16548588..16569152
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003800
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00617

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003800 RA
      MIQSSIRSF FTKVKDNEE SVEKDGKIA VSDGGPLQA 
      KQSGKRPRK SSSADSSPD VKKACPSSA SPEKSDKEN 
      KKNVEKSPK VASPSQTPV KSLLAKDSG ENDSYNPNV 
      AKYHPLEDA TWRQGERVP YIALTRTLE EIGSISSRL 
      KIIEILSNF FRSVILLSP DDLLPSLYL CLNKLGPAY 
      EGLELGVAE TSLMKAIAS STGRSMSQI KTDCQELGD 
      LGLVAEQSK TNQRMLFQP ARLTVFGVF EKLKEIAKL 
      TGHASQTKK IEKIQTMFV ACKKSEARF LIRSLTGKL 
      RIGLAEQSV LQALALACT LNPPNSKSP VKSVNVAKK 
      IASDDFKEN YDKLALILK TTYCECPNY DMIIPRLLS 
      DGIEKLPEY CKLTPGIPL KPMLAHPTK GIQEVLHRF 
      DGLKFTCEW KYDGERAQI HISGKDVFI FSRNQENNT 
      SKYPDIISR IDKCKDEKV QSCVLDCEA VAWDRERKV 
      ILPFQILST RKRKDADES SIKVQVCVF MFDLLYLNG 
      ESLVKKPFI ERRNLLKEH FKEVEGEWC FVTSLDTNT 
      MEEVQEFLE ESIKGNCEG LMVKTLEKE ATYEIAKRS 
      HNWLKLKKD YLDSCGDTL DLVVIGGYL GKGKRTGTY 
      GGFLLACYD QESEEFQSI CKIGTGFSD EALQQHSEF 
      LKNHVIQGP KPYYRFDGS HEPDHWFDS VQVWEVKCA 
      DLSLSPVHR AGIGIVDSE KGISLRFPR FVRIREDKS 
      SEEATSSQQ VASMYSNQE QVKNQAGTS NKAAEEDFY
    CDS sequence
    • >TC003800 RA
      atgattcaa agcagcata aggtctttc tttactaaa 
      gttaaagat aatgaagag agtgtggaa aaggacgga 
      aaaattgcc gtctctgat gggggcccc ttgcaagcg 
      aaacaaagt ggtaagagg ccaaggaag tcaagcagt 
      gctgatagt tccccagat gtcaagaag gcatgtccc 
      tccagtgcc tctccagaa aagtcggat aaagagaac 
      aaaaagaat gtggaaaag tcacctaaa gtagcttcg 
      cccagtcaa acgccagta aaatctctg ctagctaaa 
      gattctggc gaaaatgat tcgtataat ccaaatgtt 
      gcaaaatat catcctctt gaagatgcc acctggagg 
      caaggtgaa cgagtgcca tatatcgcc ttaactcga 
      acattggag gaaattgga agtatatca agcagatta 
      aaaataatt gaaatttta agtaacttt ttccgttct 
      gtaatactc ctaagccct gatgatttg ttgccgtcg 
      ctttatttg tgtttgaat aagcttggc ccggcctat 
      gaaggtctt gagcttggt gttgctgaa accagtcta 
      atgaaagca attgcctct tccactggt cgatcgatg 
      tcgcaaata aaaaccgat tgtcaggaa ttaggtgac 
      cttggtttg gtcgctgaa cagtcaaaa acaaaccaa 
      agaatgctt tttcaacct gcacgtctt acagttttt 
      ggcgttttt gaaaaattg aaagaaatc gccaaattg 
      accggacac gcttcacaa actaagaaa attgaaaaa 
      attcaaaca atgttcgtg gcttgtaaa aaatcggaa 
      gcacgattt ttaatccgt tcactgact ggtaaatta 
      cgaattgga ttggcagaa cagtcagtt ttgcaagca 
      ctggctttg gcgtgtact ctaaacccg ccaaattcg 
      aaatcgcca gtgaaaagt gtgaatgta gcgaaaaaa 
      attgcctcg gatgatttc aaagagaat tatgataag 
      ttggctctg atccttaaa accacttat tgcgagtgc 
      cctaattac gacatgata attccaagg ttgttatcg 
      gacggaatt gaaaaattg cccgaatat tgcaaactc 
      actccaggg attcccctg aagcccatg ttggcgcat 
      cctacgaaa ggaatccag gaggttttg caccgattt 
      gatgggctg aagtttact tgcgaatgg aagtatgac 
      ggggagaga gctcagatt cacataagc ggcaaagac 
      gttttcata tttagccgt aatcaagaa aacaacact 
      tctaaatac cccgacatc atatctcga atagacaaa 
      tgtaaagac gaaaaggtg caatcatgt gttcttgat 
      tgtgaagct gttgcttgg gatcgagaa agaaaagtc 
      attcttcct tttcaaatt ctcagtacc cggaagcgt 
      aaagatgcg gatgaatcg tcaatcaaa gtccaagtt 
      tgtgtcttc atgtttgat cttttatac ttaaacggc 
      gaatcatta gtgaaaaaa ccgttcatc gaacgtcga 
      aatctccta aaagagcat ttcaaagaa gtggaaggg 
      gagtggtgt tttgtcact tctctagat acaaacact 
      atggaagaa gtccaagaa ttcctagag gagagtatc 
      aagggaaat tgtgagggt ttaatggtg aaaactttg 
      gaaaaggaa gccacgtac gaaatcgcc aaacgctca 
      cacaattgg ctcaaactg aaaaaagac tacttggac 
      agttgtggc gatactctc gatctagtg gtcattggg 
      ggttatctg ggcaaaggg aaacggacg ggaacctac 
      ggaggcttt ctcttggct tgctatgat caagaaagc 
      gaggagttc caaagcatt tgcaagatt ggaactgga 
      ttttcggat gaagccctt caacaacat tctgaattt 
      ttgaaaaat cacgttatt cagggtcca aaaccatat 
      tacaggttt gatgggagt catgagccg gaccactgg 
      tttgattcg gtgcaagtg tgggaggtg aaatgcgcc 
      gatttgtca ctctctccc gtacacaga gcaggaatt 
      ggaattgtt gattctgag aagggaatt tcgctacga 
      ttccctaga tttgtccgt ataagagag gataagtct 
      tcagaagag gcaacatcg tcgcaacag gtcgcttca 
      atgtattct aaccaagaa caagtgaag aatcaagct 
      ggtacaagc aacaaagcg gccgaggaa gacttctat 
      taa
    mRNA sequence
    • >TC003800 RA
      cctttcaga aagaaaaaa taaattatt caattgcac 
      aatattttg tgttattca tgcattttt caaattacg 
      caaatacct tcaattaat tcgtagttg tctacctta 
      ttaattatt ttaattaaa cgtaaacca aaacattaa 
      cgtgaagga attaataaa atttataat atttttata 
      atccattcc acattcatt caaattccc atgcaccac 
      cccctctct ttttctaga cctaacctc aaaattacc 
      ctagatgtt ttggcggtt tttaagcat aaacgagca 
      aaatctaaa aagacaagc taacttgta ttcttgtgt 
      tgtgtgttt tattttaac atgattcaa agcagcata 
      aggtctttc tttactaaa gttaaagat aatgaagag 
      agtgtggaa aaggacgga aaaattgcc gtctctgat 
      gggggcccc ttgcaagcg aaacaaagt ggtaagagg 
      ccaaggaag tcaagcagt gctgatagt tccccagat 
      gtcaagaag gcatgtccc tccagtgcc tctccagaa 
      aagtcggat aaagagaac aaaaagaat gtggaaaag 
      tcacctaaa gtagcttcg cccagtcaa acgccagta 
      aaatctctg ctagctaaa gattctggc gaaaatgat 
      tcgtataat ccaaatgtt gcaaaatat catcctctt 
      gaagatgcc acctggagg caaggtgaa cgagtgcca 
      tatatcgcc ttaactcga acattggag gaaattgga 
      agtatatca agcagatta aaaataatt gaaatttta 
      agtaacttt ttccgttct gtaatactc ctaagccct 
      gatgatttg ttgccgtcg ctttatttg tgtttgaat 
      aagcttggc ccggcctat gaaggtctt gagcttggt 
      gttgctgaa accagtcta atgaaagca attgcctct 
      tccactggt cgatcgatg tcgcaaata aaaaccgat 
      tgtcaggaa ttaggtgac cttggtttg gtcgctgaa 
      cagtcaaaa acaaaccaa agaatgctt tttcaacct 
      gcacgtctt acagttttt ggcgttttt gaaaaattg 
      aaagaaatc gccaaattg accggacac gcttcacaa 
      actaagaaa attgaaaaa attcaaaca atgttcgtg 
      gcttgtaaa aaatcggaa gcacgattt ttaatccgt 
      tcactgact ggtaaatta cgaattgga ttggcagaa 
      cagtcagtt ttgcaagca ctggctttg gcgtgtact 
      ctaaacccg ccaaattcg aaatcgcca gtgaaaagt 
      gtgaatgta gcgaaaaaa attgcctcg gatgatttc 
      aaagagaat tatgataag ttggctctg atccttaaa 
      accacttat tgcgagtgc cctaattac gacatgata 
      attccaagg ttgttatcg gacggaatt gaaaaattg 
      cccgaatat tgcaaactc actccaggg attcccctg 
      aagcccatg ttggcgcat cctacgaaa ggaatccag 
      gaggttttg caccgattt gatgggctg aagtttact 
      tgcgaatgg aagtatgac ggggagaga gctcagatt 
      cacataagc ggcaaagac gttttcata tttagccgt 
      aatcaagaa aacaacact tctaaatac cccgacatc 
      atatctcga atagacaaa tgtaaagac gaaaaggtg 
      caatcatgt gttcttgat tgtgaagct gttgcttgg 
      gatcgagaa agaaaagtc attcttcct tttcaaatt 
      ctcagtacc cggaagcgt aaagatgcg gatgaatcg 
      tcaatcaaa gtccaagtt tgtgtcttc atgtttgat 
      cttttatac ttaaacggc gaatcatta gtgaaaaaa 
      ccgttcatc gaacgtcga aatctccta aaagagcat 
      ttcaaagaa gtggaaggg gagtggtgt tttgtcact 
      tctctagat acaaacact atggaagaa gtccaagaa 
      ttcctagag gagagtatc aagggaaat tgtgagggt 
      ttaatggtg aaaactttg gaaaaggaa gccacgtac 
      gaaatcgcc aaacgctca cacaattgg ctcaaactg 
      aaaaaagac tacttggac agttgtggc gatactctc 
      gatctagtg gtcattggg ggttatctg ggcaaaggg 
      aaacggacg ggaacctac ggaggcttt ctcttggct 
      tgctatgat caagaaagc gaggagttc caaagcatt 
      tgcaagatt ggaactgga ttttcggat gaagccctt 
      caacaacat tctgaattt ttgaaaaat cacgttatt 
      cagggtcca aaaccatat tacaggttt gatgggagt 
      catgagccg gaccactgg tttgattcg gtgcaagtg 
      tgggaggtg aaatgcgcc gatttgtca ctctctccc 
      gtacacaga gcaggaatt ggaattgtt gattctgag 
      aagggaatt tcgctacga ttccctaga tttgtccgt 
      ataagagag gataagtct tcagaagag gcaacatcg 
      tcgcaacag gtcgcttca atgtattct aaccaagaa 
      caagtgaag aatcaagct ggtacaagc aacaaagcg 
      gccgaggaa gacttctat taagcaaaa taattgatc 
      aagtgaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY