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Gene information
General information
TC003799 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid21: 365742..407623
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003799
    iBeetle screen iB_00616
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003799 RA
      MSGLSGTES SPELSDSGG RALKLQTDT PHLVNIGGD 
      RLSTSVTLH PIPQGRVTL GCGPGVDIR VQGTGVLPL 
      HCHIENSEG VVTIYPLSD NLSVDGVKI SRPTRLSQG 
      VMLTIGRSN YMRFNHPAE AKIMKSVLP NPRISMASI 
      TFEPDNSFQ SKYNKKPPA VPRKNSKES LIESGVEEP 
      VPSSIMTKV SKFEYLAQQ NFKKSISPK VFSSNLVTV 
      NTPAKDVLG KSPPDLQNF SKNLPQNAL NYSELNYNE 
      KQQIKTSER FGKKSPQYV NVTVSEKKN INNKVIIYE 
      NGCIPKNPN LECNELNNK SSSHKNINM NRALTPSPN 
      YNRNPSPHL RSTTPNNCK NETEITENC NDAELKRTQ 
      AQQERIKEQ EIGKAEQAR LEEILNMCV EYEKQAQSE 
      KNKLTPNRI KTNGSLPRD KRQYGPLSP VYTTPPPSP 
      RDVEIVQHE NRRNNSSYE NISINRDNS LERINANYD 
      NVEMKQVDD FRTTKCNSP YENIVPAHQ SPRTRIKTI 
      ASSNRDGKS KDTAEIIEN KFAILEAEQ YLLQNLTIN 
      DEKSLETDK ILAGRENTK FIFPLEEKS FTSLPANLN 
      ATPYNKKPK PNNINDKQK IEKIRKERK EILSQISSI 
      KRHIAETEI HEEELQRDI ELENALISG EHKAKLLEL 
      DKLEHRKNK LFNCAQKLE ESMSHRQTK QDEDQLKCK 
      EKLELAQEN MLQIEGKLA KTPKTDPKY ENVFEEYLK 
      AQEILDNEK KAFEDLEFH HLEEEADWL ASREEVQRE 
      ILDLSKKID SVKDQIQEL EQQKYNISK NNNTEVKII 
      RMRRDEFMR KLEKCSFVL RRIDNDLSV FAHQESDQE 
      ESTDSDSDK SKDLDKHLS NLSLNVIHD LSCSVIVSN 
      TKTSIDHVY NMSQSFNEK LLQEKSVLE TGIGTKCPS 
      QDDIDRISK VTSSAPISI NEEQGSLGR KTIESLKDI 
      ERKRHLHLC QQGSQVIEQ ERQRVLALK QRVQHEVRI 
      KWAQSRQDC VVPESGEKR NSVEIVGRQ PERPVSKSP 
      TSNEQIRSG SSEADSPRP MSEISEIST DFGEISNKK 
      RTKPHGDKQ RPLTRYLPI RGSDLDLRQ HIESAGHQV 
      VLCPHVIIN SSSCRGFLH KKGSKLNGW SKRWFVFDR 
      NKHTLTYYN DKSEKKARG GAYFQAIEE VYLDHLNSV 
      KSPNPQLTF IVKTHEKSY YLLAPSPEA MRIWVDVIF 
      TGAEGYQEF GHGT
    CDS sequence
    • >TC003799 RA
      atgtccggc ctaagtgga accgaatct tcgccggag 
      ttatcagac agtggaggc agagccctg aaattgcaa 
      accgacaca ccacacttg gtcaacatt gggggcgac 
      agactcagc acttcggtg acgcttcac cccattcct 
      caaggtcgg gtgacgtta ggatgcggt ccaggtgtt 
      gatattcga gtgcaaggg actggagtc ctgcctttg 
      cattgccat atcgaaaat agtgaaggt gttgttacc 
      atttatccg ctatcggat aatctctct gttgatgga 
      gtgaaaata tccagaccc actcgccta tctcagggt 
      gttatgctg accatcggc cgatcaaac tacatgcgt 
      tttaaccac ccggcggaa gcaaaaata atgaaatca 
      gttctcccc aaccctcgc atctccatg gcttcgatc 
      actttcgaa cccgacaat tcttttcag tctaaatac 
      aacaaaaaa ccaccagca gtgcctcga aaaaactcg 
      aaagaatcc ctcattgaa tcgggtgtc gaagaaccg 
      gtgccttcc agtattatg accaaagtg tcaaaattc 
      gagtattta gcccaacaa aatttcaaa aaaagcata 
      tcaccgaaa gtgttctct tcaaatctc gtaactgta 
      aatacgccg gcgaaagac gtcctcgga aaatcgcca 
      cctgatctg caaaacttc agcaagaat ttaccacaa 
      aatgcctta aactactcc gaactgaat tacaacgaa 
      aaacaacaa attaaaaca tcggagcgg tttggcaaa 
      aagtcgccg cagtacgtt aacgtgacg gtgagcgag 
      aagaaaaac attaataat aaagtgatc atttacgaa 
      aatggatgt attccgaaa aacccaaac ctcgaatgc 
      aacgaactg aataataaa agctcaagc cacaaaaat 
      attaacatg aatcgagcg ctcacacca tcgcccaac 
      tataacagg aatccttcg ccacatttg aggagcaca 
      acgccgaat aattgtaag aacgaaacc gaaattaca 
      gaaaactgc aacgacgct gaactgaaa aggacacag 
      gcgcagcaa gaacgaatc aaggagcaa gaaatcggg 
      aaagcggaa caggcaaga cttgaagaa attctaaat 
      atgtgcgtt gaatacgaa aagcaggca caatcggaa 
      aaaaataaa ctcacgccg aatagaata aaaacaaac 
      ggttcgctg ccgcgtgat aagcgtcag tatggaccc 
      ttatctcct gtgtatacc actcctcca ccttcgccg 
      agagacgtg gaaattgtt cagcatgag aacaggagg 
      aataattct tcgtatgag aatattagt attaatagg 
      gataattca ctggaacga attaatgcc aattatgat 
      aatgttgaa atgaagcaa gttgatgac tttaggact 
      acgaagtgt aacagtccg tatgaaaat attgttccg 
      gcgcatcaa tcgccaagg acgaggatt aagactatc 
      gcttcgtcg aaccgagac ggcaaatct aaggacact 
      gccgaaata atcgaaaac aaattcgcc attctcgaa 
      gccgagcaa tacctcctc cagaatctc acaataaac 
      gacgaaaaa tctctcgaa accgacaaa attctcgct 
      ggccgagaa aacacaaaa ttcatattt cccctcgaa 
      gaaaaatcc ttcacctca ctccctgcc aatttaaac 
      gccacgcca tacaacaaa aaaccaaaa ccaaacaac 
      ataaacgac aaacaaaaa atcgaaaaa atccgaaaa 
      gaacgcaaa gaaattctc tcgcaaatc tcgtccatc 
      aagcgccac atcgccgaa acggaaatc cacgaagag 
      gaactgcag cgcgacatt gagctcgaa aacgccctc 
      atttccggc gaacacaaa gcaaaattg ctcgaactg 
      gacaaattg gaacaccgc aagaacaaa ctcttcaac 
      tgtgcgcaa aaactggaa gagagtatg tcacaccga 
      cagacgaaa caagacgaa gatcagtta aaatgcaaa 
      gaaaagctc gaactagcg caggaaaat atgcttcaa 
      atcgaaggc aagttggcc aagacgccc aaaaccgac 
      cccaagtac gagaacgtc ttcgaggag tatttgaaa 
      gctcaagag attttggac aacgagaag aaagcgttc 
      gaagacttg gagttccac catctggag gaggaagcc 
      gattggttg gccagtcgg gaagaggtc caacgggaa 
      attctggat ttgtccaaa aagattgac tcggttaaa 
      gaccaaatc caggaattg gaacaacaa aagtacaac 
      atctcgaag aacaacaac actgaagtg aagatcatt 
      cgcatgcga cgtgacgaa ttcatgcgc aagttggag 
      aagtgttcg ttcgttttg cggcgaatt gacaacgat 
      ttgtccgtt ttcgcccat caggagtcc gaccaagag 
      gaatcaact gacagcgac agcgacaaa tcgaaagac 
      ctggacaaa catttgtcc aatctatcg ttgaatgtg 
      atccatgac ctgagctgt tcggtcatc gtgagcaac 
      accaagacc tcaattgac cacgtgtac aacatgtcg 
      cagtcattt aacgaaaaa ttgttgcaa gaaaaatcg 
      gttttggag accgggatt ggcaccaaa tgtccaagt 
      caggatgac attgaccgg atcagtaaa gtaacatca 
      agtgcccct attagtatc aatgaggag caaggatct 
      ctgggaaga aagacaatc gaatcgctt aaagatatc 
      gaaaggaag agacatctt catttgtgc caacaaggt 
      tcgcaggtt atcgagcag gagagacaa agagtgctg 
      gctttgaaa cagagggtg cagcatgaa gtgaggatc 
      aagtgggcg cagagcagg caggattgt gtggttcct 
      gagtcagga gagaagaga aacagtgtt gaaatcgta 
      ggacgacaa cccgaaagg cccgtttca aaatctcct 
      acgtcaaac gaacagata cgctccggc tcaagtgag 
      gcggattcg ccacgtcca atgtctgaa attagtgaa 
      ataagcacc gatttcgga gaaatctca aacaagaaa 
      agaacaaaa ccccatggc gacaaacaa cgaccctta 
      acccgatat ttgcccatc agaggctca gacctcgac 
      ctacgccaa catatagaa tcggcaggt catcaagtg 
      gtgttatgt ccccacgtc atcatcaat tcgagcagc 
      tgcagaggc tttttgcac aaaaaaggg tcaaaattg 
      aacggatgg tcgaaaagg tggttcgtt ttcgatcga 
      aataagcat acgctgact tactacaac gacaagagt 
      gaaaagaaa gctcgtgga ggagcctat tttcaagcg 
      attgaagag gtctatttg gaccacctc aacagtgtt 
      aaatcgccg aacccccaa ctgaccttc atcgtgaaa 
      acgcacgaa aagtcctat tatctactg gcgccctcg 
      cccgaagcg atgaggatt tgggttgat gttatcttt 
      actggtgct gaagggtac caagaattt ggacatggt 
      acttga
    mRNA sequence
    • >TC003799 RA
      taacatgac aacgtgagc gtatgtcga gtggtagaa 
      cacaacaca agcacgaag ttcgtgcac ctaaaattt 
      ttctgttta caattcact tttagatct agcagcagt 
      gtagtccga gtgtgatgt gtttgtttt gtgaataaa 
      tgacaatcg aggcgttct aaactactt gatgaaata 
      aataattat gaattgtaa agtgtttac aagctatta 
      aagtgttcg ttttcactg ctggaatta aactttaga 
      gatgtccgg cctaagtgg aaccgaatc ttcgccgga 
      gttatcaga cagtggagg cagagccct gaaattgca 
      aaccgacac accacactt ggtcaacat tgggggcga 
      cagactcag cacttcggt gacgcttca ccccattcc 
      tcaaggtcg ggtgacgtt aggatgcgg tccaggtgt 
      tgatattcg agtgcaagg gactggagt cctgccttt 
      gcattgcca tatcgaaaa tagtgaagg tgttgttac 
      catttatcc gctatcgga taatctctc tgttgatgg 
      agtgaaaat atccagacc cactcgcct atctcaggg 
      tgttatgct gaccatcgg ccgatcaaa ctacatgcg 
      ttttaacca cccggcgga agcaaaaat aatgaaatc 
      agttctccc caaccctcg catctccat ggcttcgat 
      cactttcga acccgacaa ttcttttca gtctaaata 
      caacaaaaa accaccagc agtgcctcg aaaaaactc 
      gaaagaatc cctcattga atcgggtgt cgaagaacc 
      ggtgccttc cagtattat gaccaaagt gtcaaaatt 
      cgagtattt agcccaaca aaatttcaa aaaaagcat 
      atcaccgaa agtgttctc ttcaaatct cgtaactgt 
      aaatacgcc ggcgaaaga cgtcctcgg aaaatcgcc 
      acctgatct gcaaaactt cagcaagaa tttaccaca 
      aaatgcctt aaactactc cgaactgaa ttacaacga 
      aaaacaaca aattaaaac atcggagcg gtttggcaa 
      aaagtcgcc gcagtacgt taacgtgac ggtgagcga 
      gaagaaaaa cattaataa taaagtgat catttacga 
      aaatggatg tattccgaa aaacccaaa cctcgaatg 
      caacgaact gaataataa aagctcaag ccacaaaaa 
      tattaacat gaatcgagc gctcacacc atcgcccaa 
      ctataacag gaatccttc gccacattt gaggagcac 
      aacgccgaa taattgtaa gaacgaaac cgaaattac 
      agaaaactg caacgacgc tgaactgaa aaggacaca 
      ggcgcagca agaacgaat caaggagca agaaatcgg 
      gaaagcgga acaggcaag acttgaaga aattctaaa 
      tatgtgcgt tgaatacga aaagcaggc acaatcgga 
      aaaaaataa actcacgcc gaatagaat aaaaacaaa 
      cggttcgct gccgcgtga taagcgtca gtatggacc 
      cttatctcc tgtgtatac cactcctcc accttcgcc 
      gagagacgt ggaaattgt tcagcatga gaacaggag 
      gaataattc ttcgtatga gaatattag tattaatag 
      ggataattc actggaacg aattaatgc caattatga 
      taatgttga aatgaagca agttgatga ctttaggac 
      tacgaagtg taacagtcc gtatgaaaa tattgttcc 
      ggcgcatca atcgccaag gacgaggat taagactat 
      cgcttcgtc gaaccgaga cggcaaatc taaggacac 
      tgccgaaat aatcgaaaa caaattcgc cattctcga 
      agccgagca atacctcct ccagaatct cacaataaa 
      cgacgaaaa atctctcga aaccgacaa aattctcgc 
      tggccgaga aaacacaaa attcatatt tcccctcga 
      agaaaaatc cttcacctc actccctgc caatttaaa 
      cgccacgcc atacaacaa aaaaccaaa accaaacaa 
      cataaacga caaacaaaa aatcgaaaa aatccgaaa 
      agaacgcaa agaaattct ctcgcaaat ctcgtccat 
      caagcgcca catcgccga aacggaaat ccacgaaga 
      ggaactgca gcgcgacat tgagctcga aaacgccct 
      catttccgg cgaacacaa agcaaaatt gctcgaact 
      ggacaaatt ggaacaccg caagaacaa actcttcaa 
      ctgtgcgca aaaactgga agagagtat gtcacaccg 
      acagacgaa acaagacga agatcagtt aaaatgcaa 
      agaaaagct cgaactagc gcaggaaaa tatgcttca 
      aatcgaagg caagttggc caagacgcc caaaaccga 
      ccccaagta cgagaacgt cttcgagga gtatttgaa 
      agctcaaga gattttgga caacgagaa gaaagcgtt 
      cgaagactt ggagttcca ccatctgga ggaggaagc 
      cgattggtt ggccagtcg ggaagaggt ccaacggga 
      aattctgga tttgtccaa aaagattga ctcggttaa 
      agaccaaat ccaggaatt ggaacaaca aaagtacaa 
      catctcgaa gaacaacaa cactgaagt gaagatcat 
      tcgcatgcg acgtgacga attcatgcg caagttgga 
      gaagtgttc gttcgtttt gcggcgaat tgacaacga 
      tttgtccgt tttcgccca tcaggagtc cgaccaaga 
      ggaatcaac tgacagcga cagcgacaa atcgaaaga 
      cctggacaa acatttgtc caatctatc gttgaatgt 
      gatccatga cctgagctg ttcggtcat cgtgagcaa 
      caccaagac ctcaattga ccacgtgta caacatgtc 
      gcagtcatt taacgaaaa attgttgca agaaaaatc 
      ggttttgga gaccgggat tggcaccaa atgtccaag 
      tcaggatga cattgaccg gatcagtaa agtaacatc 
      aagtgcccc tattagtat caatgagga gcaaggatc 
      tctgggaag aaagacaat cgaatcgct taaagatat 
      cgaaaggaa gagacatct tcatttgtg ccaacaagg 
      ttcgcaggt tatcgagca ggagagaca aagagtgct 
      ggctttgaa acagagggt gcagcatga agtgaggat 
      caagtgggc gcagagcag gcaggattg tgtggttcc 
      tgagtcagg agagaagag aaacagtgt tgaaatcgt 
      aggacgaca acccgaaag gcccgtttc aaaatctcc 
      tacgtcaaa cgaacagat acgctccgg ctcaagtga 
      ggcggattc gccacgtcc aatgtctga aattagtga 
      aataagcac cgatttcgg agaaatctc aaacaagaa 
      aagaacaaa accccatgg cgacaaaca acgaccctt 
      aacccgata tttgcccat cagaggctc agacctcga 
      cctacgcca acatataga atcggcagg tcatcaagt 
      ggtgttatg tccccacgt catcatcaa ttcgagcag 
      ctgcagagg ctttttgca caaaaaagg gtcaaaatt 
      gaacggatg gtcgaaaag gtggttcgt tttcgatcg 
      aaataagca tacgctgac ttactacaa cgacaagag 
      tgaaaagaa agctcgtgg aggagccta ttttcaagc 
      gattgaaga ggtctattt ggaccacct caacagtgt 
      taaatcgcc gaaccccca actgacctt catcgtgaa 
      aacgcacga aaagtccta ttatctact ggcgccctc 
      gcccgaagc gatgaggat ttgggttga tgttatctt 
      tactggtgc tgaagggta ccaagaatt tggacatgg 
      tacttgaaa cttggtacg attacgtat ttggcacgt 
      aaaattatt gttaatttt gtaaatttg tggcgaaat 
      tgtcaattc tctacttaa gtgattgga ttaatttaa 
      gtaagtgcc gattaaata gtgtttgtt gcagaagtg 
      aataatatt ttaccattt ttacgaata gagtatttt 
      tctatttta aatgttggc aatattgcc gcctacgaa 
      tatacctaa tttattaac gatgtagtg ccttttgaa 
      gagactgtt ttatacatt tataaaatt tgcgcaaat 
      ctgtatctc taggtatta aggaaaatt gtgatgtcc 
      gtatgtatt accaactaa aaataaaac atgtagcaa 
      agc