| General information | |||
| TC003797 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid21: 478863..500985 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC003797 | ||
| General information | ||||
| iB_00615 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
leg musculature coxal musculature present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC003797 RA
MGKKAYDLE SSEKNGKKD ALTPEFVQE PSEKLEKAP PVGFFKLFR YATKWDTFL MIIAVFASI GTGILQPLN TLLFGDLTG TIVDYVFTI NSNETSEEQ KQNATDVFI DGITDFAVY NTLIGVGML VLSYISTEF FNYTALKQV FKVRTLYLE KVFNQDISW YDVNNTGDF SSRMSDDLS KFEDGIGEK VPMFVHFQA TFLASLIMA LVKGWQLAL ICLVSLPLS MIAIGIIAV LTSKLAKKE QDAYGSAGS IAEEVLTSI RTVIAFGGQ HKEITRYDE ELEFAKKNN IKRQSMTAI GFGLLWFFI YGSYALAFW YGVKLVLED RNKPAKDKV YDPGTMVTV FFSVMTGSM NFGISSPYI EAFGVARAA ASKVYQIID NIPKINLSK GNGDKIDNL KGDIKFRNV RFVYPSRQD VPILLGLDL DIKAGQTVA LVGSSGCGK STCIQLIQR FYDPLEGEV SLDGKNLKD FDLTWLRNN IGVVGQEPV LFATTIAEN IRYGNSKAT DEEIKNAAI KANAHEFIK KLPSGYDTL VGERGAQLS GGQKQRIAI ARALVRNPA ILLLDEATS ALDTNSEAK VQAALDKAS KGCTTVIVA HRLSTIRNA NKIVVISKG KVVEQGTHN ELMELKSEY YNLVMTQVS AVEKFDGDQ EGESRKLVE LERQVSLLD DEKHDDAEE EVQEAERSV SLMSILRMN KPEWVSISI GCIASIVMG CSMPAFAVI FGDIMGVLA EKNEDEVIS ETNRFCIYF VIAGVVSGI ATFLQIFMF SVAGEKLTM RLRSMTFIA MLKQEMGWY DRKDNGVGA LCARLSGEA AHVQGATGQ RVGTILQSI ATIGLSVGL SMYYQWKLG LVALAFTPF ILLAVFFQH RLMNVENEA HHKSLQKSN KLAVEAVGN VRTVVSLGL EETFHKLYI SYLMEHHKR TLRNTHFRA VVLGLARSI MFFAYSACM YYGGHLIRD EGLLYQDVF KVSQSLIMG TVSIANALA FTPNLQKGL VAAARIIRL LRRQPLIRD EPGAKDKEW ENGAIQYDT IYFSYPTRP NIMVLKGLN LSVLQGKTV ALVGPSGCG KSTIIQLIE RFYDPLEGT LTVDNEDIR NIRLGSHRS HLGIVSQEP NLFDRTIGD NIAYGDNSR EVTQEEIIE AAKNANIHN FIASLPLGY ETRLGEKGT QLSGGQKQR VAIARALVR NPKLLLLDE ATSALDSES EKVVQEALD NAKKGRTCI TIAHRLTTI QDADVICVI DKGVVAEIG THSELLSQK GLYYKLHSL QNK
- >TC003797 RA
atggggaag aaagcgtac gatttggaa tcaagcgaa aaaaatgga aaaaaagac gccctaacg cccgaattt gtacaagaa ccaagcgaa aaactggaa aaagcacct cctgttgga ttctttaaa ctatttcgc tatgccaca aaatgggac acatttctg atgatcatt gcggttttt gcatccatt ggcactgga attttgcaa cctttaaac acacttttg tttggagac ttgacagga accatcgtc gattatgtc ttcaccatt aattcaaat gaaaccagc gaggaacaa aaacaaaac gcgactgat gtttttatc gacggaatc acagatttt gcagtctat aacacctta ataggggtt ggaatgcta gttttgagt tatatttca acagagttc ttcaactac actgccctg aaacaagtt tttaaagta cgaacatta tatctagaa aaagtattc aatcaagac atttcgtgg tacgatgtc aataatact ggggatttt tcgagccga atgtcagac gatctctcg aaatttgag gacggaatt ggggaaaaa gtcccaatg tttgtccat ttccaagcc actttcctg gcctctctg atcatggct ttggtcaaa ggttggcaa cttgccctc atctgtctc gtctcgcta cccttgtcc atgattgca attggaatc atcgcagtg ttgacatca aaacttgca aaaaaagag caagacgcg tacggttct gccggttca atcgccgaa gaagtcttg acttcaatt cgaacagtt atagctttc ggaggacaa cacaaagaa ataacacgc tatgacgaa gagttggag tttgcaaag aagaacaat atcaaaaga cagtccatg acagcgatt ggtttcggt ctattgtgg tttttcatc tatggcagt tacgctctt gcgttttgg tatggagtt aagttggtt ctagaagac agaaacaaa ccagccaag gacaaagtc tacgatcct ggtaccatg gtcactgtg tttttcagt gtgatgact ggtagtatg aatttcgga atttcctca ccgtatatt gaagcgttt ggtgttgca agagctgca gccagcaaa gtttaccaa atcattgac aacattccc aaaatcaat ctatcgaaa ggcaatgga gataaaatt gataatctc aagggagac attaaattt aggaacgtt cggtttgtg tatccctcc agacaagat gttccaatt ttgctaggt ctggatttg gacatcaaa gctggccaa actgttgct ttggttggc agttcgggt tgtggaaaa tcgacttgt atccaactg atccagcgt ttttatgac cctcttgaa ggagaggtg agtctggac ggaaaaaac ttgaaagac tttgacttg acttggctg aggaacaac attggtgtc gtgggccag gagccagtt ctcttcgct accacaata gccgagaac attcgttac ggcaacagc aaagccaca gacgaagaa atcaaaaac gccgcaatc aaagccaac gctcacgaa tttatcaaa aaactcccc tcaggttac gacacttta gttggtgaa agaggagcg caattgtcc ggagggcaa aaacaaaga attgcgatc gctagagct cttgtcaga aatcctgcg attctgctc ttggacgaa gcaacttcg gctctggat acgaacagc gaggctaaa gttcaagcc gctttggat aaagctagt aaaggttgt acgactgtt attgtggct catcggttg tcgacgatt cgaaatgcc aataaaatt gttgtgata tcgaagggg aaagttgtg gagcaggga actcataac gagttgatg gaacttaaa agcgagtat tataatctg gttatgacg caagtaagt gctgtggag aagtttgat ggggatcag gaaggtgaa agtaggaag ttagtggag ctggaaagg caggtttcg cttctggat gacgaaaag cacgacgat gctgaggaa gaagtccaa gaagccgaa agatccgta tcgctcatg tcaatatta agaatgaac aaaccagaa tgggtctca atttccatc ggctgcatc gcttccatc gtgatgggt tgttccatg ccggcgttt gctgtcata ttcggtgat attatgggg gttttagct gagaaaaac gaagacgaa gttatttct gaaaccaac cgtttctgc atctacttt gtgattgct ggagttgtt tctggaatc gcaacgttt ttacaaata tttatgttt agtgtcgct ggtgaaaaa ctaacaatg aggcttcgc agcatgaca ttcatagca atgttaaag caagagatg ggctggtat gatagaaaa gataacgga gttggcgcc ctttgtgct agattatct ggtgaagcg gcacatgtc caaggagcg actggtcaa agagttgga acaatttta caatcgatt gccacaatt ggtttatca gtgggtctc tcaatgtac taccagtgg aaattggga cttgtggcg ttggctttt acgcctttt atcctcctt gctgttttc ttccaacac agattaatg aacgtcgaa aatgaagct catcacaag tccttacaa aaatctaac aaacttgca gttgaagcg gtaggtaat gtgcgtacc gtagtgtcg ttagggctc gaagaaact ttccacaag ctttacatc tcgtatctg atggaacac cacaaaaga acactaaga aatacacat tttcgtgcc gtggtgtta ggtctagcc cggagtata atgttcttt gcttattct gcttgtatg tattacggt ggtcatctt attcgagac gaaggatta ctatatcag gatgttttt aaagtatca cagtcactt attatgggt acggtatca atcgccaat gctctagcg tttacacca aatctccaa aaaggtcta gtagcagct gctagaatc ataaggttg ctcaggcga caaccactc atcagggat gaacccggt gctaaagat aaagaatgg gaaaacggt gccatccaa tatgacacc atatacttc tcgtaccca acccgtcca aacatcatg gtcctcaaa ggcctaaat ttgtccgta ttgcaagga aaaactgta gccctagtc ggccctagc ggctgtgga aaatcgaca ataatccaa ctaattgaa cgattttac gaccctctt gaaggcact cttacagta gacaacgaa gatatcaga aacatccga ctaggatcg catcgttcc cacttggga atagtctcg caagagcca aatcttttc gatagaacc atcggcgac aacatcgct tatggtgac aattccagg gaagtgact caagaagaa ataatagaa gctgccaaa aacgcaaac atacacaat ttcatcgct tcgttacct ttgggttat gaaactcgt cttggtgaa aaaggaaca caattgtca ggtggccag aaacagaga gttgctatc gctagagct ttggttaga aatccgaaa ttattgctt ttggacgaa gcgacaagt gctttagat tccgaaagt gaaaaggtt gtgcaagaa gctttagat aatgcaaag aagggaaga acttgtatc acaatagcc cacagactg acaaccatt caagatgct gatgttatt tgtgtgata gacaagggt gtggtggca gaaattgga acacattcc gaacttttg agccagaag ggactctac tacaaattg cattcgctc cagaacaaa tag
- >TC003797 RA
tgtaatata tgtgatact tatggtagt tctctcttc aaccctgat cattagttt acaacacaa ttacacata atggggaag aaagcgtac gatttggaa tcaagcgaa aaaaatgga aaaaaagac gccctaacg cccgaattt gtacaagaa ccaagcgaa aaactggaa aaagcacct cctgttgga ttctttaaa ctatttcgc tatgccaca aaatgggac acatttctg atgatcatt gcggttttt gcatccatt ggcactgga attttgcaa cctttaaac acacttttg tttggagac ttgacagga accatcgtc gattatgtc ttcaccatt aattcaaat gaaaccagc gaggaacaa aaacaaaac gcgactgat gtttttatc gacggaatc acagatttt gcagtctat aacacctta ataggggtt ggaatgcta gttttgagt tatatttca acagagttc ttcaactac actgccctg aaacaagtt tttaaagta cgaacatta tatctagaa aaagtattc aatcaagac atttcgtgg tacgatgtc aataatact ggggatttt tcgagccga atgtcagac gatctctcg aaatttgag gacggaatt ggggaaaaa gtcccaatg tttgtccat ttccaagcc actttcctg gcctctctg atcatggct ttggtcaaa ggttggcaa cttgccctc atctgtctc gtctcgcta cccttgtcc atgattgca attggaatc atcgcagtg ttgacatca aaacttgca aaaaaagag caagacgcg tacggttct gccggttca atcgccgaa gaagtcttg acttcaatt cgaacagtt atagctttc ggaggacaa cacaaagaa ataacacgc tatgacgaa gagttggag tttgcaaag aagaacaat atcaaaaga cagtccatg acagcgatt ggtttcggt ctattgtgg tttttcatc tatggcagt tacgctctt gcgttttgg tatggagtt aagttggtt ctagaagac agaaacaaa ccagccaag gacaaagtc tacgatcct ggtaccatg gtcactgtg tttttcagt gtgatgact ggtagtatg aatttcgga atttcctca ccgtatatt gaagcgttt ggtgttgca agagctgca gccagcaaa gtttaccaa atcattgac aacattccc aaaatcaat ctatcgaaa ggcaatgga gataaaatt gataatctc aagggagac attaaattt aggaacgtt cggtttgtg tatccctcc agacaagat gttccaatt ttgctaggt ctggatttg gacatcaaa gctggccaa actgttgct ttggttggc agttcgggt tgtggaaaa tcgacttgt atccaactg atccagcgt ttttatgac cctcttgaa ggagaggtg agtctggac ggaaaaaac ttgaaagac tttgacttg acttggctg aggaacaac attggtgtc gtgggccag gagccagtt ctcttcgct accacaata gccgagaac attcgttac ggcaacagc aaagccaca gacgaagaa atcaaaaac gccgcaatc aaagccaac gctcacgaa tttatcaaa aaactcccc tcaggttac gacacttta gttggtgaa agaggagcg caattgtcc ggagggcaa aaacaaaga attgcgatc gctagagct cttgtcaga aatcctgcg attctgctc ttggacgaa gcaacttcg gctctggat acgaacagc gaggctaaa gttcaagcc gctttggat aaagctagt aaaggttgt acgactgtt attgtggct catcggttg tcgacgatt cgaaatgcc aataaaatt gttgtgata tcgaagggg aaagttgtg gagcaggga actcataac gagttgatg gaacttaaa agcgagtat tataatctg gttatgacg caagtaagt gctgtggag aagtttgat ggggatcag gaaggtgaa agtaggaag ttagtggag ctggaaagg caggtttcg cttctggat gacgaaaag cacgacgat gctgaggaa gaagtccaa gaagccgaa agatccgta tcgctcatg tcaatatta agaatgaac aaaccagaa tgggtctca atttccatc ggctgcatc gcttccatc gtgatgggt tgttccatg ccggcgttt gctgtcata ttcggtgat attatgggg gttttagct gagaaaaac gaagacgaa gttatttct gaaaccaac cgtttctgc atctacttt gtgattgct ggagttgtt tctggaatc gcaacgttt ttacaaata tttatgttt agtgtcgct ggtgaaaaa ctaacaatg aggcttcgc agcatgaca ttcatagca atgttaaag caagagatg ggctggtat gatagaaaa gataacgga gttggcgcc ctttgtgct agattatct ggtgaagcg gcacatgtc caaggagcg actggtcaa agagttgga acaatttta caatcgatt gccacaatt ggtttatca gtgggtctc tcaatgtac taccagtgg aaattggga cttgtggcg ttggctttt acgcctttt atcctcctt gctgttttc ttccaacac agattaatg aacgtcgaa aatgaagct catcacaag tccttacaa aaatctaac aaacttgca gttgaagcg gtaggtaat gtgcgtacc gtagtgtcg ttagggctc gaagaaact ttccacaag ctttacatc tcgtatctg atggaacac cacaaaaga acactaaga aatacacat tttcgtgcc gtggtgtta ggtctagcc cggagtata atgttcttt gcttattct gcttgtatg tattacggt ggtcatctt attcgagac gaaggatta ctatatcag gatgttttt aaagtatca cagtcactt attatgggt acggtatca atcgccaat gctctagcg tttacacca aatctccaa aaaggtcta gtagcagct gctagaatc ataaggttg ctcaggcga caaccactc atcagggat gaacccggt gctaaagat aaagaatgg gaaaacggt gccatccaa tatgacacc atatacttc tcgtaccca acccgtcca aacatcatg gtcctcaaa ggcctaaat ttgtccgta ttgcaagga aaaactgta gccctagtc ggccctagc ggctgtgga aaatcgaca ataatccaa ctaattgaa cgattttac gaccctctt gaaggcact cttacagta gacaacgaa gatatcaga aacatccga ctaggatcg catcgttcc cacttggga atagtctcg caagagcca aatcttttc gatagaacc atcggcgac aacatcgct tatggtgac aattccagg gaagtgact caagaagaa ataatagaa gctgccaaa aacgcaaac atacacaat ttcatcgct tcgttacct ttgggttat gaaactcgt cttggtgaa aaaggaaca caattgtca ggtggccag aaacagaga gttgctatc gctagagct ttggttaga aatccgaaa ttattgctt ttggacgaa gcgacaagt gctttagat tccgaaagt gaaaaggtt gtgcaagaa gctttagat aatgcaaag aagggaaga acttgtatc acaatagcc cacagactg acaaccatt caagatgct gatgttatt tgtgtgata gacaagggt gtggtggca gaaattgga acacattcc gaacttttg agccagaag ggactctac tacaaattg cattcgctc cagaacaaa tagtacaat ttttaatta cgatgacaa ttatgtaca aatatactt atttattta tgtgttgta ggcaattgt aataaagct attttttta aa