Status
Please wait ...
Gene information
General information
TC003797 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 16710052..16732174
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003797
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00615

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 leg musculature sometimes not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    leg musculature coxal musculature present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003797 RA
      MGKKAYDLE SSEKNGKKD ALTPEFVQE PSEKLEKAP 
      PVGFFKLFR YATKWDTFL MIIAVFASI GTGILQPLN 
      TLLFGDLTG TIVDYVFTI NSNETSEEQ KQNATDVFI 
      DGITDFAVY NTLIGVGML VLSYISTEF FNYTALKQV 
      FKVRTLYLE KVFNQDISW YDVNNTGDF SSRMSDDLS 
      KFEDGIGEK VPMFVHFQA TFLASLIMA LVKGWQLAL 
      ICLVSLPLS MIAIGIIAV LTSKLAKKE QDAYGSAGS 
      IAEEVLTSI RTVIAFGGQ HKEITRYDE ELEFAKKNN 
      IKRQSMTAI GFGLLWFFI YGSYALAFW YGVKLVLED 
      RNKPAKDKV YDPGTMVTV FFSVMTGSM NFGISSPYI 
      EAFGVARAA ASKVYQIID NIPKINLSK GNGDKIDNL 
      KGDIKFRNV RFVYPSRQD VPILLGLDL DIKAGQTVA 
      LVGSSGCGK STCIQLIQR FYDPLEGEV SLDGKNLKD 
      FDLTWLRNN IGVVGQEPV LFATTIAEN IRYGNSKAT 
      DEEIKNAAI KANAHEFIK KLPSGYDTL VGERGAQLS 
      GGQKQRIAI ARALVRNPA ILLLDEATS ALDTNSEAK 
      VQAALDKAS KGCTTVIVA HRLSTIRNA NKIVVISKG 
      KVVEQGTHN ELMELKSEY YNLVMTQVS AVEKFDGDQ 
      EGESRKLVE LERQVSLLD DEKHDDAEE EVQEAERSV 
      SLMSILRMN KPEWVSISI GCIASIVMG CSMPAFAVI 
      FGDIMGVLA EKNEDEVIS ETNRFCIYF VIAGVVSGI 
      ATFLQIFMF SVAGEKLTM RLRSMTFIA MLKQEMGWY 
      DRKDNGVGA LCARLSGEA AHVQGATGQ RVGTILQSI 
      ATIGLSVGL SMYYQWKLG LVALAFTPF ILLAVFFQH 
      RLMNVENEA HHKSLQKSN KLAVEAVGN VRTVVSLGL 
      EETFHKLYI SYLMEHHKR TLRNTHFRA VVLGLARSI 
      MFFAYSACM YYGGHLIRD EGLLYQDVF KVSQSLIMG 
      TVSIANALA FTPNLQKGL VAAARIIRL LRRQPLIRD 
      EPGAKDKEW ENGAIQYDT IYFSYPTRP NIMVLKGLN 
      LSVLQGKTV ALVGPSGCG KSTIIQLIE RFYDPLEGT 
      LTVDNEDIR NIRLGSHRS HLGIVSQEP NLFDRTIGD 
      NIAYGDNSR EVTQEEIIE AAKNANIHN FIASLPLGY 
      ETRLGEKGT QLSGGQKQR VAIARALVR NPKLLLLDE 
      ATSALDSES EKVVQEALD NAKKGRTCI TIAHRLTTI 
      QDADVICVI DKGVVAEIG THSELLSQK GLYYKLHSL 
      QNK
    CDS sequence
    • >TC003797 RA
      atggggaag aaagcgtac gatttggaa tcaagcgaa 
      aaaaatgga aaaaaagac gccctaacg cccgaattt 
      gtacaagaa ccaagcgaa aaactggaa aaagcacct 
      cctgttgga ttctttaaa ctatttcgc tatgccaca 
      aaatgggac acatttctg atgatcatt gcggttttt 
      gcatccatt ggcactgga attttgcaa cctttaaac 
      acacttttg tttggagac ttgacagga accatcgtc 
      gattatgtc ttcaccatt aattcaaat gaaaccagc 
      gaggaacaa aaacaaaac gcgactgat gtttttatc 
      gacggaatc acagatttt gcagtctat aacacctta 
      ataggggtt ggaatgcta gttttgagt tatatttca 
      acagagttc ttcaactac actgccctg aaacaagtt 
      tttaaagta cgaacatta tatctagaa aaagtattc 
      aatcaagac atttcgtgg tacgatgtc aataatact 
      ggggatttt tcgagccga atgtcagac gatctctcg 
      aaatttgag gacggaatt ggggaaaaa gtcccaatg 
      tttgtccat ttccaagcc actttcctg gcctctctg 
      atcatggct ttggtcaaa ggttggcaa cttgccctc 
      atctgtctc gtctcgcta cccttgtcc atgattgca 
      attggaatc atcgcagtg ttgacatca aaacttgca 
      aaaaaagag caagacgcg tacggttct gccggttca 
      atcgccgaa gaagtcttg acttcaatt cgaacagtt 
      atagctttc ggaggacaa cacaaagaa ataacacgc 
      tatgacgaa gagttggag tttgcaaag aagaacaat 
      atcaaaaga cagtccatg acagcgatt ggtttcggt 
      ctattgtgg tttttcatc tatggcagt tacgctctt 
      gcgttttgg tatggagtt aagttggtt ctagaagac 
      agaaacaaa ccagccaag gacaaagtc tacgatcct 
      ggtaccatg gtcactgtg tttttcagt gtgatgact 
      ggtagtatg aatttcgga atttcctca ccgtatatt 
      gaagcgttt ggtgttgca agagctgca gccagcaaa 
      gtttaccaa atcattgac aacattccc aaaatcaat 
      ctatcgaaa ggcaatgga gataaaatt gataatctc 
      aagggagac attaaattt aggaacgtt cggtttgtg 
      tatccctcc agacaagat gttccaatt ttgctaggt 
      ctggatttg gacatcaaa gctggccaa actgttgct 
      ttggttggc agttcgggt tgtggaaaa tcgacttgt 
      atccaactg atccagcgt ttttatgac cctcttgaa 
      ggagaggtg agtctggac ggaaaaaac ttgaaagac 
      tttgacttg acttggctg aggaacaac attggtgtc 
      gtgggccag gagccagtt ctcttcgct accacaata 
      gccgagaac attcgttac ggcaacagc aaagccaca 
      gacgaagaa atcaaaaac gccgcaatc aaagccaac 
      gctcacgaa tttatcaaa aaactcccc tcaggttac 
      gacacttta gttggtgaa agaggagcg caattgtcc 
      ggagggcaa aaacaaaga attgcgatc gctagagct 
      cttgtcaga aatcctgcg attctgctc ttggacgaa 
      gcaacttcg gctctggat acgaacagc gaggctaaa 
      gttcaagcc gctttggat aaagctagt aaaggttgt 
      acgactgtt attgtggct catcggttg tcgacgatt 
      cgaaatgcc aataaaatt gttgtgata tcgaagggg 
      aaagttgtg gagcaggga actcataac gagttgatg 
      gaacttaaa agcgagtat tataatctg gttatgacg 
      caagtaagt gctgtggag aagtttgat ggggatcag 
      gaaggtgaa agtaggaag ttagtggag ctggaaagg 
      caggtttcg cttctggat gacgaaaag cacgacgat 
      gctgaggaa gaagtccaa gaagccgaa agatccgta 
      tcgctcatg tcaatatta agaatgaac aaaccagaa 
      tgggtctca atttccatc ggctgcatc gcttccatc 
      gtgatgggt tgttccatg ccggcgttt gctgtcata 
      ttcggtgat attatgggg gttttagct gagaaaaac 
      gaagacgaa gttatttct gaaaccaac cgtttctgc 
      atctacttt gtgattgct ggagttgtt tctggaatc 
      gcaacgttt ttacaaata tttatgttt agtgtcgct 
      ggtgaaaaa ctaacaatg aggcttcgc agcatgaca 
      ttcatagca atgttaaag caagagatg ggctggtat 
      gatagaaaa gataacgga gttggcgcc ctttgtgct 
      agattatct ggtgaagcg gcacatgtc caaggagcg 
      actggtcaa agagttgga acaatttta caatcgatt 
      gccacaatt ggtttatca gtgggtctc tcaatgtac 
      taccagtgg aaattggga cttgtggcg ttggctttt 
      acgcctttt atcctcctt gctgttttc ttccaacac 
      agattaatg aacgtcgaa aatgaagct catcacaag 
      tccttacaa aaatctaac aaacttgca gttgaagcg 
      gtaggtaat gtgcgtacc gtagtgtcg ttagggctc 
      gaagaaact ttccacaag ctttacatc tcgtatctg 
      atggaacac cacaaaaga acactaaga aatacacat 
      tttcgtgcc gtggtgtta ggtctagcc cggagtata 
      atgttcttt gcttattct gcttgtatg tattacggt 
      ggtcatctt attcgagac gaaggatta ctatatcag 
      gatgttttt aaagtatca cagtcactt attatgggt 
      acggtatca atcgccaat gctctagcg tttacacca 
      aatctccaa aaaggtcta gtagcagct gctagaatc 
      ataaggttg ctcaggcga caaccactc atcagggat 
      gaacccggt gctaaagat aaagaatgg gaaaacggt 
      gccatccaa tatgacacc atatacttc tcgtaccca 
      acccgtcca aacatcatg gtcctcaaa ggcctaaat 
      ttgtccgta ttgcaagga aaaactgta gccctagtc 
      ggccctagc ggctgtgga aaatcgaca ataatccaa 
      ctaattgaa cgattttac gaccctctt gaaggcact 
      cttacagta gacaacgaa gatatcaga aacatccga 
      ctaggatcg catcgttcc cacttggga atagtctcg 
      caagagcca aatcttttc gatagaacc atcggcgac 
      aacatcgct tatggtgac aattccagg gaagtgact 
      caagaagaa ataatagaa gctgccaaa aacgcaaac 
      atacacaat ttcatcgct tcgttacct ttgggttat 
      gaaactcgt cttggtgaa aaaggaaca caattgtca 
      ggtggccag aaacagaga gttgctatc gctagagct 
      ttggttaga aatccgaaa ttattgctt ttggacgaa 
      gcgacaagt gctttagat tccgaaagt gaaaaggtt 
      gtgcaagaa gctttagat aatgcaaag aagggaaga 
      acttgtatc acaatagcc cacagactg acaaccatt 
      caagatgct gatgttatt tgtgtgata gacaagggt 
      gtggtggca gaaattgga acacattcc gaacttttg 
      agccagaag ggactctac tacaaattg cattcgctc 
      cagaacaaa tag
    mRNA sequence
    • >TC003797 RA
      tgtaatata tgtgatact tatggtagt tctctcttc 
      aaccctgat cattagttt acaacacaa ttacacata 
      atggggaag aaagcgtac gatttggaa tcaagcgaa 
      aaaaatgga aaaaaagac gccctaacg cccgaattt 
      gtacaagaa ccaagcgaa aaactggaa aaagcacct 
      cctgttgga ttctttaaa ctatttcgc tatgccaca 
      aaatgggac acatttctg atgatcatt gcggttttt 
      gcatccatt ggcactgga attttgcaa cctttaaac 
      acacttttg tttggagac ttgacagga accatcgtc 
      gattatgtc ttcaccatt aattcaaat gaaaccagc 
      gaggaacaa aaacaaaac gcgactgat gtttttatc 
      gacggaatc acagatttt gcagtctat aacacctta 
      ataggggtt ggaatgcta gttttgagt tatatttca 
      acagagttc ttcaactac actgccctg aaacaagtt 
      tttaaagta cgaacatta tatctagaa aaagtattc 
      aatcaagac atttcgtgg tacgatgtc aataatact 
      ggggatttt tcgagccga atgtcagac gatctctcg 
      aaatttgag gacggaatt ggggaaaaa gtcccaatg 
      tttgtccat ttccaagcc actttcctg gcctctctg 
      atcatggct ttggtcaaa ggttggcaa cttgccctc 
      atctgtctc gtctcgcta cccttgtcc atgattgca 
      attggaatc atcgcagtg ttgacatca aaacttgca 
      aaaaaagag caagacgcg tacggttct gccggttca 
      atcgccgaa gaagtcttg acttcaatt cgaacagtt 
      atagctttc ggaggacaa cacaaagaa ataacacgc 
      tatgacgaa gagttggag tttgcaaag aagaacaat 
      atcaaaaga cagtccatg acagcgatt ggtttcggt 
      ctattgtgg tttttcatc tatggcagt tacgctctt 
      gcgttttgg tatggagtt aagttggtt ctagaagac 
      agaaacaaa ccagccaag gacaaagtc tacgatcct 
      ggtaccatg gtcactgtg tttttcagt gtgatgact 
      ggtagtatg aatttcgga atttcctca ccgtatatt 
      gaagcgttt ggtgttgca agagctgca gccagcaaa 
      gtttaccaa atcattgac aacattccc aaaatcaat 
      ctatcgaaa ggcaatgga gataaaatt gataatctc 
      aagggagac attaaattt aggaacgtt cggtttgtg 
      tatccctcc agacaagat gttccaatt ttgctaggt 
      ctggatttg gacatcaaa gctggccaa actgttgct 
      ttggttggc agttcgggt tgtggaaaa tcgacttgt 
      atccaactg atccagcgt ttttatgac cctcttgaa 
      ggagaggtg agtctggac ggaaaaaac ttgaaagac 
      tttgacttg acttggctg aggaacaac attggtgtc 
      gtgggccag gagccagtt ctcttcgct accacaata 
      gccgagaac attcgttac ggcaacagc aaagccaca 
      gacgaagaa atcaaaaac gccgcaatc aaagccaac 
      gctcacgaa tttatcaaa aaactcccc tcaggttac 
      gacacttta gttggtgaa agaggagcg caattgtcc 
      ggagggcaa aaacaaaga attgcgatc gctagagct 
      cttgtcaga aatcctgcg attctgctc ttggacgaa 
      gcaacttcg gctctggat acgaacagc gaggctaaa 
      gttcaagcc gctttggat aaagctagt aaaggttgt 
      acgactgtt attgtggct catcggttg tcgacgatt 
      cgaaatgcc aataaaatt gttgtgata tcgaagggg 
      aaagttgtg gagcaggga actcataac gagttgatg 
      gaacttaaa agcgagtat tataatctg gttatgacg 
      caagtaagt gctgtggag aagtttgat ggggatcag 
      gaaggtgaa agtaggaag ttagtggag ctggaaagg 
      caggtttcg cttctggat gacgaaaag cacgacgat 
      gctgaggaa gaagtccaa gaagccgaa agatccgta 
      tcgctcatg tcaatatta agaatgaac aaaccagaa 
      tgggtctca atttccatc ggctgcatc gcttccatc 
      gtgatgggt tgttccatg ccggcgttt gctgtcata 
      ttcggtgat attatgggg gttttagct gagaaaaac 
      gaagacgaa gttatttct gaaaccaac cgtttctgc 
      atctacttt gtgattgct ggagttgtt tctggaatc 
      gcaacgttt ttacaaata tttatgttt agtgtcgct 
      ggtgaaaaa ctaacaatg aggcttcgc agcatgaca 
      ttcatagca atgttaaag caagagatg ggctggtat 
      gatagaaaa gataacgga gttggcgcc ctttgtgct 
      agattatct ggtgaagcg gcacatgtc caaggagcg 
      actggtcaa agagttgga acaatttta caatcgatt 
      gccacaatt ggtttatca gtgggtctc tcaatgtac 
      taccagtgg aaattggga cttgtggcg ttggctttt 
      acgcctttt atcctcctt gctgttttc ttccaacac 
      agattaatg aacgtcgaa aatgaagct catcacaag 
      tccttacaa aaatctaac aaacttgca gttgaagcg 
      gtaggtaat gtgcgtacc gtagtgtcg ttagggctc 
      gaagaaact ttccacaag ctttacatc tcgtatctg 
      atggaacac cacaaaaga acactaaga aatacacat 
      tttcgtgcc gtggtgtta ggtctagcc cggagtata 
      atgttcttt gcttattct gcttgtatg tattacggt 
      ggtcatctt attcgagac gaaggatta ctatatcag 
      gatgttttt aaagtatca cagtcactt attatgggt 
      acggtatca atcgccaat gctctagcg tttacacca 
      aatctccaa aaaggtcta gtagcagct gctagaatc 
      ataaggttg ctcaggcga caaccactc atcagggat 
      gaacccggt gctaaagat aaagaatgg gaaaacggt 
      gccatccaa tatgacacc atatacttc tcgtaccca 
      acccgtcca aacatcatg gtcctcaaa ggcctaaat 
      ttgtccgta ttgcaagga aaaactgta gccctagtc 
      ggccctagc ggctgtgga aaatcgaca ataatccaa 
      ctaattgaa cgattttac gaccctctt gaaggcact 
      cttacagta gacaacgaa gatatcaga aacatccga 
      ctaggatcg catcgttcc cacttggga atagtctcg 
      caagagcca aatcttttc gatagaacc atcggcgac 
      aacatcgct tatggtgac aattccagg gaagtgact 
      caagaagaa ataatagaa gctgccaaa aacgcaaac 
      atacacaat ttcatcgct tcgttacct ttgggttat 
      gaaactcgt cttggtgaa aaaggaaca caattgtca 
      ggtggccag aaacagaga gttgctatc gctagagct 
      ttggttaga aatccgaaa ttattgctt ttggacgaa 
      gcgacaagt gctttagat tccgaaagt gaaaaggtt 
      gtgcaagaa gctttagat aatgcaaag aagggaaga 
      acttgtatc acaatagcc cacagactg acaaccatt 
      caagatgct gatgttatt tgtgtgata gacaagggt 
      gtggtggca gaaattgga acacattcc gaacttttg 
      agccagaag ggactctac tacaaattg cattcgctc 
      cagaacaaa tagtacaat ttttaatta cgatgacaa 
      ttatgtaca aatatactt atttattta tgtgttgta 
      ggcaattgt aataaagct attttttta aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY