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Gene information
General information
TC003787 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid21: 906880..912785
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003787
    iBeetle screen iB_00614
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003787 RA
      MAALNEAYT GDVHGVRGA DYSCYREAR 
      RAGLRGTFR AFLSSRVQN VDSIVRLAD 
      RKLPVSNLR GEVLFNSWS EMFKGDGAP 
      FPHPPRIYS FNGKNVLTD LSWPHKAVW 
      HGALVNGER ALDTSCDAW HSASRDKVG 
      LAGSLKGPT LLEQTPYTC DKKLILLCI 
      EATSELFVQ RKRRDLDSS NDEILNEDD 
      YHTLLSLIN
    CDS sequence
    • >TC003787 RA
      atggcggca ctgaacgag gcgtacacc ggagacgtt 
      cacggcgtt cgcggcgca gattactcc tgttatcgc 
      gaagctcga cgtgcgggc ttgcgagga actttccga 
      gcttttcta tcctctcgc gttcaaaac gtcgattcc 
      attgtgcga ctagcggat cgcaagctg cctgtatct 
      aatctccga ggtgaagta cttttcaac tcctggtcg 
      gagatgttc aaaggggac ggcgcgcca ttcccgcat 
      ccgccgcgc atttactct tttaatggc aagaacgta 
      ttaactgat ctttcgtgg ccgcacaaa gccgtatgg 
      catggggcg ctggtgaat ggcgaaagg gctctggac 
      acgtcatgt gacgcctgg cactcggcc tccagagac 
      aaagtgggc cttgctgga tcgctcaaa ggacccaca 
      cttctagag caaacgccc tacacttgc gacaagaaa 
      ctcatcttg ctttgcatt gaggccacc agtgaactg 
      ttcgtccaa aggaaacgg agggattta gatagcagt 
      aacgacgaa atactgaac gaggacgat tatcacact 
      ctactaagt ctcatcaat tga
    mRNA sequence
    • >TC003787 RA
      tccttggca tcaagtctc atatgtgtt gacataatt 
      ttcagttgc gtatggcgg cactgaacg aggcgtaca 
      ccggagacg ttcacggcg ttcgcggcg cagattact 
      cctgttatc gcgaagctc gacgtgcgg gcttgcgag 
      gaactttcc gagcttttc tatcctctc gcgttcaaa 
      acgtcgatt ccattgtgc gactagcgg atcgcaagc 
      tgcctgtat ctaatctcc gaggtgaag tacttttca 
      actcctggt cggagatgt tcaaagggg acggcgcgc 
      cattcccgc atccgccgc gcatttact cttttaatg 
      gcaagaacg tattaactg atctttcgt ggccgcaca 
      aagccgtat ggcatgggg cgctggtga atggcgaaa 
      gggctctgg acacgtcat gtgacgcct ggcactcgg 
      cctccagag acaaagtgg gccttgctg gatcgctca 
      aaggaccca cacttctag agcaaacgc cctacactt 
      gcgacaaga aactcatct tgctttgca ttgaggcca 
      ccagtgaac tgttcgtcc aaaggaaac ggagggatt 
      tagatagca gtaacgacg aaatactga acgaggacg 
      attatcaca ctctactaa gtctcatca attgaagaa 
      aaattttgt gattggtca acgaccgtt ccatccata 
      gtcagacat cgataattc cgtcatctc tagtacaca 
      tgtccaaca atttaactt taagcttaa atacataat 
      tatctataa gatacgaaa cctacgtaa taatacaaa 
      attaatgta cccaaatag ttgtattcc gttgtcttt 
      agagaattt atatagttt ttataatca tttcattgt 
      ttaaagcaa acacaagga aattaaaag gaaacaacg 
      ttaaataaa ttcctttta agttccgaa actcgtaaa 
      tgtacttat cgttttcgc gcgtatgcc gttctttag 
      ctttctcta aatgtataa atattgttt ttatatctg 
      actacatat cagatttaa attaagtta tgttatgac 
      aatgtgtgt atgttaaag ttgtgttca atggactga 
      ttgtaaata cttgtgagt tgcaaaatg ttttggtgt 
      aaatgttac taaatagta ttgaatgcc atgtatttg 
      taataaatt atttataaa tgt