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Gene information
General information
TC003783 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17426404..17484732
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003783
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00613

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    mature egg not present - Number of animals showing the phenotype: 2

    mature egg degenerated - Number of animals showing the phenotype: 2
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003783 RA
      MRRFPTTQP DAVYSYGLR PEGSRKPWS PSEDGESAL 
SRSMRYADP WLYGSVRAP AGLVLAPAF VLCTCSDYV 
NGTKRSSKK APTTCKKCK GTRLPLSPS ESKFGTVRC 
YPTSTTSTP ARAGTMRVL SSSRPSILP AADPYDLMR 
RSRLASRSA SPKGAPRGR RSILECDIN PYELMTAES 
SPSITLVNG QRIRVRPPT ADQEYEDVQ VKRPAPKVP 
PNKKPNEKE SECNRFKSI LKKPTTFSD TDSNSRERS 
PSPAHKSGS HFYLPMPGN TPRKKVQFL VENELEVDQ 
NRKEEEASE DAEMSNEEG QETSDQKVC KLRRSVSER 
FNHGSKKVV FHDTMALRS RPSRSELRL DLGEEDLER 
LDGLPSKTR RKSPTNPRP SQPPPPPPP ASPPVEKPK 
PTSKCAKPA KVAPQEEPN VKTQKAKKV AVKEPMEKQ 
EVGNVDEIK GKVEEKTQE VAVPPSVPP RKRSNNKQP 
HVNIIQTSA SVQSVHVES PSVHSVQIE SSALISEPA 
NKTVVQING SPTVHKLQI KNEFADVVN VQTVSVLDN 
PQRKTSIMI TGDDCYSTV NVNDDVPLY QSSVVVNDT 
ANEKTVIQN TKKSSSVYI TGNFAASES SECSKEPKA 
NESNKNGSH KYDDCELIE TAQITHKQE NNILLDTFD 
QTNIPSSEL LKELLKDPV EAVRRNLVP HVCGKSDVS 
RRPRPKKFV FEETHLEAS DNTNGINNF IEESLLRMK 
NLDLNDDLN SEHSSSTQY ELMDASSEC YTDHSNRSS 
VTEEELANR TKFYELLAD SAVVEVSES EDHHYESIK 
NNSDPIYEE IEIPPPLPA NPPPLSLLD DLHLDKEYT 
TRSIFEGAS KYDILSYLV DAKERGIVQ EDNYSYNFA 
NNGISVDDD TKETYNRVC HMSTVNDSS EDNSLIMPS 
NLIDDKVPF QKTAEVERN DSGVGSETS KSSLSKYRM 
KQETATTCE DCEAVLESS QDTEPLICR KCLKKRSER 
KEIITEIVE TEEKYSRDL QIILEEFYQ PMLVAGLLT 
PEQLSAIFL NVEELLENS QSLAERLRD AVEIAVEQG 
DEDLLTVNI GKIFLEAAP MLHGFETYC VRQGAASLL 
LASLEKEKE LLRIFLRVS QMENTMLRR MNLNSFLMV 
PVQRVTKYP LLLARLYKV TPVHHETRE QLKEAQHKI 
ELHLNHMNS ETKDVPSKL WRRIGSSSG RRSSTEMDL 
VNIKLRKIA VDVLEWNHD EAKFALEGK LLFTQPTDN 
NWRKGRTIK LTPVNALLV INGKPSMNR PERTDENGL 
IFSRHSAAR EAALLLVRD KNGRYSLLR EPLYLDRCV 
IAADPAWQS YFEVQELLG KDTFIFKAE DEEQTKIWY 
SQLQFHAQG MGAWRKRRN ALANIMING MGLRS
    CDS sequence
    • >TC003783 RA
      atgcgacga tttccaacg actcagcct gatgccgtg 
tattcttac ggtctaagg cccgaagga tctcgcaaa 
ccctggtct ccatccgaa gacggagag agcgctcta 
tcccgctcg atgcgttat gccgacccc tggctctac 
ggcagcgtg cgcgcacca gctggccta gtcctcgcc 
ccagcgttc gtcctctgc acctgctcc gactacgtc 
aacggcacc aaacgctcc tccaagaag gccccaacg 
acatgcaaa aagtgcaaa ggcacgcgc ctcccgctc 
tccccctcc gagagcaag ttcgggacc gtgcgatgc 
taccccacc tccaccacc tccacgccc gcgcgggcc 
ggcaccatg cgggtgctg agctcctcc aggccctcc 
atcctgccg gccgccgac ccctacgac ctcatgcgg 
cgctcgcgc ctcgcctcg cgctccgcc tcccccaag 
ggggcccct cgggggcgg cgctccatc ctcgagtgc 
gacatcaac ccctacgag ctcatgacg gccgagagc 
tcccccagc atcacgctg gtcaacggc cagaggatc 
agggtgcgg cccccgacg gcggaccag gagtacgag 
gacgtgcag gtgaagaga ccggcccca aaagtgccc 
ccgaacaaa aagccaaac gaaaaggaa tctgagtgc 
aaccggttc aagtctata ttaaagaaa ccaacaact 
ttcagtgac acggattca aacagccgt gagcgcagc 
ccctcgccc gcgcacaaa agtgggtca cacttttac 
ctgccaatg cccggcaac acacccagg aaaaaagtg 
cagtttttg gttgaaaat gaacttgaa gtggaccaa 
aatcgaaag gaggaggag gcgtcggaa gacgccgaa 
atgtcaaac gaggaagga caagaaacc tccgatcaa 
aaagtctgc aagttgcga cggagcgtc agcgaacgg 
ttcaatcat ggctcgaag aaagttgta ttccatgat 
acaatggca ttacgttca cgtccctcc cggagcgaa 
ctgagacta gacttaggc gaggaagat ctcgagcga 
ttagacggc ctgccatcg aaaaccagg cgaaaaagc 
cccacaaat ccacggcca agtcaaccg cccccgccg 
ccgccgccc gcctcgccc ccggttgaa aaacccaaa 
cccacatcg aaatgtgcg aaaccagcg aaggtcgca 
cctcaagaa gaaccaaat gtgaaaaca caaaaagct 
aagaaggtt gcagtcaaa gaaccaatg gaaaagcaa 
gaagttgga aatgtggac gagattaag ggaaaagtt 
gaggagaaa acgcaggaa gttgcggtt ccaccgtct 
gttcctcct cggaaaagg agcaacaat aaacaaccc 
cacgtgaat ataattcag acgtctgcg agtgttcag 
tccgtccat gttgagtca ccaagtgtt cattctgtt 
caaattgag tcatcggct ttgataagt gaacccgca 
aacaaaacg gtggttcaa attaacggc tcgccgacg 
gttcacaaa ttgcagatt aagaacgaa ttcgcagat 
gtcgtgaac gttcaaacg gtttccgtg cttgataat 
ccgcaaagg aagacatcc atcatgata acaggtgac 
gattgttat tcaaccgta aacgttaat gatgacgtt 
ccactttat caatcttcg gtcgttgtg aacgatacg 
gcgaacgag aaaactgta attcaaaat accaaaaag 
agcagttcc gtttacatc accggtaat ttcgccgcg 
tctgaaagc agcgaatgc tcgaaagag cccaaagcc 
aatgaatcg aacaaaaac ggaagccat aagtacgac 
gattgcgaa ttaatcgaa actgcacaa ataacgcac 
aaacaggag aataatatc ctcttggac acgttcgac 
caaaccaat attccctcc tcggagctg ctcaaggag 
ctgctcaaa gaccccgtt gaagccgtg cggcggaat 
ttagtccct catgtctgt ggtaaatca gacgtgtca 
aggagacca aggcccaag aagttcgtt tttgaagaa 
acgcatctg gaagcgagt gataacacg aacggtatc 
aacaacttc atcgaagag tcgctcctc agaatgaag 
aatttggat ttgaatgat gatctaaac tcggaacat 
agttcgtcg actcagtac gagctcatg gacgccagt 
tctgagtgt tatacagat cacagtaat cgtagctcg 
gttactgag gaagaattg gcaaatagg acgaagttt 
tatgagttg ttggctgac tcggcggtt gttgaagtc 
tcagaaagt gaggatcat cattacgaa agtattaag 
aataattcg gacccgatt tatgaagaa attgagatt 
ccgccgcca ctgccggca aacccccca ccgttgagt 
cttctagac gatctgcat ctggacaag gaatacacc 
acgaggtcc atattcgag ggagcatcg aaatacgac 
attttaagt tacttggtc gacgcaaaa gagcgcgga 
atcgtccaa gaagacaat tactcgtac aatttcgcc 
aacaatgga atttcagtt gacgatgat accaaagaa 
acgtacaac agagtctgc catatgagc actgtgaac 
gattccagt gaagacaac agtctgatc atgccgagt 
aatttaatc gacgacaaa gtaccattc caaaaaacg 
gcagaagtc gagagaaac gactccggc gttggttcc 
gaaaccagc aagagttcc ctcagcaaa taccggatg 
aagcaagaa acggccaca acttgcgaa gactgcgag 
gcagttctt gagtcgtct caagacacc gagcccttg 
atttgccgc aagtgcttg aaaaagcgc tcggaacgg 
aaggaaatc atcaccgaa atcgtggag actgaggag 
aagtacagt cgcgatttg cagattatt ttggaggag 
ttttaccaa ccgatgtta gtggctggg ttattgacg 
ccggagcaa ctatcggcg attttcctc aacgtggag 
gaacttctg gaaaacagt caaagtctg gcggaacgg 
ttgcgagac gctgtggaa attgcagtc gaacaagga 
gatgaagat ctcctaact gttaacatc gggaagatt 
ttcctggag gcggccccc atgttgcac ggatttgag 
acctattgc gtccggcaa ggcgcagca agtctgctt 
ttagctagt ctagagaag gaaaaagag ctcctcagg 
atattcctc cgggtgtcg caaatggag aacacgatg 
ctgagaagg atgaatttg aattcgttc ctcatggta 
cctgtccaa cgtgtcact aaatacccg ctgttgttg 
gcgcggttg tataaggtt acgccggtg caccacgag 
acgagggaa cagttgaag gaagcgcaa cacaagatt 
gagttacat ctgaatcac atgaactcc gaaacgaag 
gacgtgccg agtaagctg tggaggagg attgggagt 
agttccggg aggaggtca agcaccgaa atggatttg 
gttaatata aagttgagg aagattgcg gttgatgtg 
ttggagtgg aatcatgat gaggccaag tttgcgttg 
gaggggaaa ttgctgttt acgcaaccg acggataat 
aattggagg aaggggagg acgatcaag ctgacgccg 
gttaatgcc cttcttgtg atcaatggc aagccatca 
atgaatcga ccggaacga accgacgag aatgggttg 
atattttca cggcattct gcggcccgt gaagcggct 
cttcttctg gtgcgggac aagaacggt cggtattcc 
cttcttcga gagccgctt tatctggac cgctgcgtc 
atcgccgca gatccggcg tggcaaagc tacttcgaa 
gtacaagaa ctcctagga aaggatact ttcattttt 
aaggctgaa gacgaggag caaacgaaa atctggtat 
agtcaatta cagtttcac gcccaagga atgggagcc 
tggaggaaa cggcgtaat gccctagcc aatataatg 
ataaatggc atgggctta aggtcctaa
    mRNA sequence
    • >TC003783 RA
      taataacag taaaaaatt aaaacttgt cacaattat 
tgtgatagc aggagcagt gaggcataa caaatgaac 
aaaatgtag gtgtctacc tgataataa ccgagtttt 
ttgttacag gaaagtgaa actggaaga ggtagaaaa 
ccattacaa acaaagtga tgccaaagc ggacacatt 
ctcatggct gcagtggtt agccatgca atgcgacga 
tttccaacg actcagcct gatgccgtg tattcttac 
ggtctaagg cccgaagga tctcgcaaa ccctggtct 
ccatccgaa gacggagag agcgctcta tcccgctcg 
atgcgttat gccgacccc tggctctac ggcagcgtg 
cgcgcacca gctggccta gtcctcgcc ccagcgttc 
gtcctctgc acctgctcc gactacgtc aacggcacc 
aaacgctcc tccaagaag gccccaacg acatgcaaa 
aagtgcaaa ggcacgcgc ctcccgctc tccccctcc 
gagagcaag ttcgggacc gtgcgatgc taccccacc 
tccaccacc tccacgccc gcgcgggcc ggcaccatg 
cgggtgctg agctcctcc aggccctcc atcctgccg 
gccgccgac ccctacgac ctcatgcgg cgctcgcgc 
ctcgcctcg cgctccgcc tcccccaag ggggcccct 
cgggggcgg cgctccatc ctcgagtgc gacatcaac 
ccctacgag ctcatgacg gccgagagc tcccccagc 
atcacgctg gtcaacggc cagaggatc agggtgcgg 
cccccgacg gcggaccag gagtacgag gacgtgcag 
gtgaagaga ccggcccca aaagtgccc ccgaacaaa 
aagccaaac gaaaaggaa tctgagtgc aaccggttc 
aagtctata ttaaagaaa ccaacaact ttcagtgac 
acggattca aacagccgt gagcgcagc ccctcgccc 
gcgcacaaa agtgggtca cacttttac ctgccaatg 
cccggcaac acacccagg aaaaaagtg cagtttttg 
gttgaaaat gaacttgaa gtggaccaa aatcgaaag 
gaggaggag gcgtcggaa gacgccgaa atgtcaaac 
gaggaagga caagaaacc tccgatcaa aaagtctgc 
aagttgcga cggagcgtc agcgaacgg ttcaatcat 
ggctcgaag aaagttgta ttccatgat acaatggca 
ttacgttca cgtccctcc cggagcgaa ctgagacta 
gacttaggc gaggaagat ctcgagcga ttagacggc 
ctgccatcg aaaaccagg cgaaaaagc cccacaaat 
ccacggcca agtcaaccg cccccgccg ccgccgccc 
gcctcgccc ccggttgaa aaacccaaa cccacatcg 
aaatgtgcg aaaccagcg aaggtcgca cctcaagaa 
gaaccaaat gtgaaaaca caaaaagct aagaaggtt 
gcagtcaaa gaaccaatg gaaaagcaa gaagttgga 
aatgtggac gagattaag ggaaaagtt gaggagaaa 
acgcaggaa gttgcggtt ccaccgtct gttcctcct 
cggaaaagg agcaacaat aaacaaccc cacgtgaat 
ataattcag acgtctgcg agtgttcag tccgtccat 
gttgagtca ccaagtgtt cattctgtt caaattgag 
tcatcggct ttgataagt gaacccgca aacaaaacg 
gtggttcaa attaacggc tcgccgacg gttcacaaa 
ttgcagatt aagaacgaa ttcgcagat gtcgtgaac 
gttcaaacg gtttccgtg cttgataat ccgcaaagg 
aagacatcc atcatgata acaggtgac gattgttat 
tcaaccgta aacgttaat gatgacgtt ccactttat 
caatcttcg gtcgttgtg aacgatacg gcgaacgag 
aaaactgta attcaaaat accaaaaag agcagttcc 
gtttacatc accggtaat ttcgccgcg tctgaaagc 
agcgaatgc tcgaaagag cccaaagcc aatgaatcg 
aacaaaaac ggaagccat aagtacgac gattgcgaa 
ttaatcgaa actgcacaa ataacgcac aaacaggag 
aataatatc ctcttggac acgttcgac caaaccaat 
attccctcc tcggagctg ctcaaggag ctgctcaaa 
gaccccgtt gaagccgtg cggcggaat ttagtccct 
catgtctgt ggtaaatca gacgtgtca aggagacca 
aggcccaag aagttcgtt tttgaagaa acgcatctg 
gaagcgagt gataacacg aacggtatc aacaacttc 
atcgaagag tcgctcctc agaatgaag aatttggat 
ttgaatgat gatctaaac tcggaacat agttcgtcg 
actcagtac gagctcatg gacgccagt tctgagtgt 
tatacagat cacagtaat cgtagctcg gttactgag 
gaagaattg gcaaatagg acgaagttt tatgagttg 
ttggctgac tcggcggtt gttgaagtc tcagaaagt 
gaggatcat cattacgaa agtattaag aataattcg 
gacccgatt tatgaagaa attgagatt ccgccgcca 
ctgccggca aacccccca ccgttgagt cttctagac 
gatctgcat ctggacaag gaatacacc acgaggtcc 
atattcgag ggagcatcg aaatacgac attttaagt 
tacttggtc gacgcaaaa gagcgcgga atcgtccaa 
gaagacaat tactcgtac aatttcgcc aacaatgga 
atttcagtt gacgatgat accaaagaa acgtacaac 
agagtctgc catatgagc actgtgaac gattccagt 
gaagacaac agtctgatc atgccgagt aatttaatc 
gacgacaaa gtaccattc caaaaaacg gcagaagtc 
gagagaaac gactccggc gttggttcc gaaaccagc 
aagagttcc ctcagcaaa taccggatg aagcaagaa 
acggccaca acttgcgaa gactgcgag gcagttctt 
gagtcgtct caagacacc gagcccttg atttgccgc 
aagtgcttg aaaaagcgc tcggaacgg aaggaaatc 
atcaccgaa atcgtggag actgaggag aagtacagt 
cgcgatttg cagattatt ttggaggag ttttaccaa 
ccgatgtta gtggctggg ttattgacg ccggagcaa 
ctatcggcg attttcctc aacgtggag gaacttctg 
gaaaacagt caaagtctg gcggaacgg ttgcgagac 
gctgtggaa attgcagtc gaacaagga gatgaagat 
ctcctaact gttaacatc gggaagatt ttcctggag 
gcggccccc atgttgcac ggatttgag acctattgc 
gtccggcaa ggcgcagca agtctgctt ttagctagt 
ctagagaag gaaaaagag ctcctcagg atattcctc 
cgggtgtcg caaatggag aacacgatg ctgagaagg 
atgaatttg aattcgttc ctcatggta cctgtccaa 
cgtgtcact aaatacccg ctgttgttg gcgcggttg 
tataaggtt acgccggtg caccacgag acgagggaa 
cagttgaag gaagcgcaa cacaagatt gagttacat 
ctgaatcac atgaactcc gaaacgaag gacgtgccg 
agtaagctg tggaggagg attgggagt agttccggg 
aggaggtca agcaccgaa atggatttg gttaatata 
aagttgagg aagattgcg gttgatgtg ttggagtgg 
aatcatgat gaggccaag tttgcgttg gaggggaaa 
ttgctgttt acgcaaccg acggataat aattggagg 
aaggggagg acgatcaag ctgacgccg gttaatgcc 
cttcttgtg atcaatggc aagccatca atgaatcga 
ccggaacga accgacgag aatgggttg atattttca 
cggcattct gcggcccgt gaagcggct cttcttctg 
gtgcgggac aagaacggt cggtattcc cttcttcga 
gagccgctt tatctggac cgctgcgtc atcgccgca 
gatccggcg tggcaaagc tacttcgaa gtacaagaa 
ctcctagga aaggatact ttcattttt aaggctgaa 
gacgaggag caaacgaaa atctggtat agtcaatta 
cagtttcac gcccaagga atgggagcc tggaggaaa 
cggcgtaat gccctagcc aatataatg ataaatggc 
atgggctta aggtcctaa tcgacgaac ttactgaaa 
ataaagatt taatgagct ttaagtcat tacaactcg 
aatgtttcc tgtaatttg tacttgatc gatataggt 
aattttatt atttctttt tgtaaatat ttagtaatt 
tagctgtga tattttccg gatgaggtt tttgggatt 
caatttatt ttacaataa taatatgct tgacgacca 
gtttatggt tattgtaac tttatatga ctttatatt 
aatcattgt aaaaacatg taattaaat aaaaataaa 
taaataatt taaaatatg
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