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Gene information
General information
TC003783 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid21: 1195215..1253543
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003783
    iBeetle screen iB_00613
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 2

    mature egg degenerated - Number of animals showing the phenotype: 2
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003783 RA
      MRRFPTTQP DAVYSYGLR PEGSRKPWS PSEDGESAL 
      SRSMRYADP WLYGSVRAP AGLVLAPAF VLCTCSDYV 
      NGTKRSSKK APTTCKKCK GTRLPLSPS ESKFGTVRC 
      YPTSTTSTP ARAGTMRVL SSSRPSILP AADPYDLMR 
      RSRLASRSA SPKGAPRGR RSILECDIN PYELMTAES 
      SPSITLVNG QRIRVRPPT ADQEYEDVQ VKRPAPKVP 
      PNKKPNEKE SECNRFKSI LKKPTTFSD TDSNSRERS 
      PSPAHKSGS HFYLPMPGN TPRKKVQFL VENELEVDQ 
      NRKEEEASE DAEMSNEEG QETSDQKVC KLRRSVSER 
      FNHGSKKVV FHDTMALRS RPSRSELRL DLGEEDLER 
      LDGLPSKTR RKSPTNPRP SQPPPPPPP ASPPVEKPK 
      PTSKCAKPA KVAPQEEPN VKTQKAKKV AVKEPMEKQ 
      EVGNVDEIK GKVEEKTQE VAVPPSVPP RKRSNNKQP 
      HVNIIQTSA SVQSVHVES PSVHSVQIE SSALISEPA 
      NKTVVQING SPTVHKLQI KNEFADVVN VQTVSVLDN 
      PQRKTSIMI TGDDCYSTV NVNDDVPLY QSSVVVNDT 
      ANEKTVIQN TKKSSSVYI TGNFAASES SECSKEPKA 
      NESNKNGSH KYDDCELIE TAQITHKQE NNILLDTFD 
      QTNIPSSEL LKELLKDPV EAVRRNLVP HVCGKSDVS 
      RRPRPKKFV FEETHLEAS DNTNGINNF IEESLLRMK 
      NLDLNDDLN SEHSSSTQY ELMDASSEC YTDHSNRSS 
      VTEEELANR TKFYELLAD SAVVEVSES EDHHYESIK 
      NNSDPIYEE IEIPPPLPA NPPPLSLLD DLHLDKEYT 
      TRSIFEGAS KYDILSYLV DAKERGIVQ EDNYSYNFA 
      NNGISVDDD TKETYNRVC HMSTVNDSS EDNSLIMPS 
      NLIDDKVPF QKTAEVERN DSGVGSETS KSSLSKYRM 
      KQETATTCE DCEAVLESS QDTEPLICR KCLKKRSER 
      KEIITEIVE TEEKYSRDL QIILEEFYQ PMLVAGLLT 
      PEQLSAIFL NVEELLENS QSLAERLRD AVEIAVEQG 
      DEDLLTVNI GKIFLEAAP MLHGFETYC VRQGAASLL 
      LASLEKEKE LLRIFLRVS QMENTMLRR MNLNSFLMV 
      PVQRVTKYP LLLARLYKV TPVHHETRE QLKEAQHKI 
      ELHLNHMNS ETKDVPSKL WRRIGSSSG RRSSTEMDL 
      VNIKLRKIA VDVLEWNHD EAKFALEGK LLFTQPTDN 
      NWRKGRTIK LTPVNALLV INGKPSMNR PERTDENGL 
      IFSRHSAAR EAALLLVRD KNGRYSLLR EPLYLDRCV 
      IAADPAWQS YFEVQELLG KDTFIFKAE DEEQTKIWY 
      SQLQFHAQG MGAWRKRRN ALANIMING MGLRS
    CDS sequence
    • >TC003783 RA
      atgcgacga tttccaacg actcagcct gatgccgtg 
      tattcttac ggtctaagg cccgaagga tctcgcaaa 
      ccctggtct ccatccgaa gacggagag agcgctcta 
      tcccgctcg atgcgttat gccgacccc tggctctac 
      ggcagcgtg cgcgcacca gctggccta gtcctcgcc 
      ccagcgttc gtcctctgc acctgctcc gactacgtc 
      aacggcacc aaacgctcc tccaagaag gccccaacg 
      acatgcaaa aagtgcaaa ggcacgcgc ctcccgctc 
      tccccctcc gagagcaag ttcgggacc gtgcgatgc 
      taccccacc tccaccacc tccacgccc gcgcgggcc 
      ggcaccatg cgggtgctg agctcctcc aggccctcc 
      atcctgccg gccgccgac ccctacgac ctcatgcgg 
      cgctcgcgc ctcgcctcg cgctccgcc tcccccaag 
      ggggcccct cgggggcgg cgctccatc ctcgagtgc 
      gacatcaac ccctacgag ctcatgacg gccgagagc 
      tcccccagc atcacgctg gtcaacggc cagaggatc 
      agggtgcgg cccccgacg gcggaccag gagtacgag 
      gacgtgcag gtgaagaga ccggcccca aaagtgccc 
      ccgaacaaa aagccaaac gaaaaggaa tctgagtgc 
      aaccggttc aagtctata ttaaagaaa ccaacaact 
      ttcagtgac acggattca aacagccgt gagcgcagc 
      ccctcgccc gcgcacaaa agtgggtca cacttttac 
      ctgccaatg cccggcaac acacccagg aaaaaagtg 
      cagtttttg gttgaaaat gaacttgaa gtggaccaa 
      aatcgaaag gaggaggag gcgtcggaa gacgccgaa 
      atgtcaaac gaggaagga caagaaacc tccgatcaa 
      aaagtctgc aagttgcga cggagcgtc agcgaacgg 
      ttcaatcat ggctcgaag aaagttgta ttccatgat 
      acaatggca ttacgttca cgtccctcc cggagcgaa 
      ctgagacta gacttaggc gaggaagat ctcgagcga 
      ttagacggc ctgccatcg aaaaccagg cgaaaaagc 
      cccacaaat ccacggcca agtcaaccg cccccgccg 
      ccgccgccc gcctcgccc ccggttgaa aaacccaaa 
      cccacatcg aaatgtgcg aaaccagcg aaggtcgca 
      cctcaagaa gaaccaaat gtgaaaaca caaaaagct 
      aagaaggtt gcagtcaaa gaaccaatg gaaaagcaa 
      gaagttgga aatgtggac gagattaag ggaaaagtt 
      gaggagaaa acgcaggaa gttgcggtt ccaccgtct 
      gttcctcct cggaaaagg agcaacaat aaacaaccc 
      cacgtgaat ataattcag acgtctgcg agtgttcag 
      tccgtccat gttgagtca ccaagtgtt cattctgtt 
      caaattgag tcatcggct ttgataagt gaacccgca 
      aacaaaacg gtggttcaa attaacggc tcgccgacg 
      gttcacaaa ttgcagatt aagaacgaa ttcgcagat 
      gtcgtgaac gttcaaacg gtttccgtg cttgataat 
      ccgcaaagg aagacatcc atcatgata acaggtgac 
      gattgttat tcaaccgta aacgttaat gatgacgtt 
      ccactttat caatcttcg gtcgttgtg aacgatacg 
      gcgaacgag aaaactgta attcaaaat accaaaaag 
      agcagttcc gtttacatc accggtaat ttcgccgcg 
      tctgaaagc agcgaatgc tcgaaagag cccaaagcc 
      aatgaatcg aacaaaaac ggaagccat aagtacgac 
      gattgcgaa ttaatcgaa actgcacaa ataacgcac 
      aaacaggag aataatatc ctcttggac acgttcgac 
      caaaccaat attccctcc tcggagctg ctcaaggag 
      ctgctcaaa gaccccgtt gaagccgtg cggcggaat 
      ttagtccct catgtctgt ggtaaatca gacgtgtca 
      aggagacca aggcccaag aagttcgtt tttgaagaa 
      acgcatctg gaagcgagt gataacacg aacggtatc 
      aacaacttc atcgaagag tcgctcctc agaatgaag 
      aatttggat ttgaatgat gatctaaac tcggaacat 
      agttcgtcg actcagtac gagctcatg gacgccagt 
      tctgagtgt tatacagat cacagtaat cgtagctcg 
      gttactgag gaagaattg gcaaatagg acgaagttt 
      tatgagttg ttggctgac tcggcggtt gttgaagtc 
      tcagaaagt gaggatcat cattacgaa agtattaag 
      aataattcg gacccgatt tatgaagaa attgagatt 
      ccgccgcca ctgccggca aacccccca ccgttgagt 
      cttctagac gatctgcat ctggacaag gaatacacc 
      acgaggtcc atattcgag ggagcatcg aaatacgac 
      attttaagt tacttggtc gacgcaaaa gagcgcgga 
      atcgtccaa gaagacaat tactcgtac aatttcgcc 
      aacaatgga atttcagtt gacgatgat accaaagaa 
      acgtacaac agagtctgc catatgagc actgtgaac 
      gattccagt gaagacaac agtctgatc atgccgagt 
      aatttaatc gacgacaaa gtaccattc caaaaaacg 
      gcagaagtc gagagaaac gactccggc gttggttcc 
      gaaaccagc aagagttcc ctcagcaaa taccggatg 
      aagcaagaa acggccaca acttgcgaa gactgcgag 
      gcagttctt gagtcgtct caagacacc gagcccttg 
      atttgccgc aagtgcttg aaaaagcgc tcggaacgg 
      aaggaaatc atcaccgaa atcgtggag actgaggag 
      aagtacagt cgcgatttg cagattatt ttggaggag 
      ttttaccaa ccgatgtta gtggctggg ttattgacg 
      ccggagcaa ctatcggcg attttcctc aacgtggag 
      gaacttctg gaaaacagt caaagtctg gcggaacgg 
      ttgcgagac gctgtggaa attgcagtc gaacaagga 
      gatgaagat ctcctaact gttaacatc gggaagatt 
      ttcctggag gcggccccc atgttgcac ggatttgag 
      acctattgc gtccggcaa ggcgcagca agtctgctt 
      ttagctagt ctagagaag gaaaaagag ctcctcagg 
      atattcctc cgggtgtcg caaatggag aacacgatg 
      ctgagaagg atgaatttg aattcgttc ctcatggta 
      cctgtccaa cgtgtcact aaatacccg ctgttgttg 
      gcgcggttg tataaggtt acgccggtg caccacgag 
      acgagggaa cagttgaag gaagcgcaa cacaagatt 
      gagttacat ctgaatcac atgaactcc gaaacgaag 
      gacgtgccg agtaagctg tggaggagg attgggagt 
      agttccggg aggaggtca agcaccgaa atggatttg 
      gttaatata aagttgagg aagattgcg gttgatgtg 
      ttggagtgg aatcatgat gaggccaag tttgcgttg 
      gaggggaaa ttgctgttt acgcaaccg acggataat 
      aattggagg aaggggagg acgatcaag ctgacgccg 
      gttaatgcc cttcttgtg atcaatggc aagccatca 
      atgaatcga ccggaacga accgacgag aatgggttg 
      atattttca cggcattct gcggcccgt gaagcggct 
      cttcttctg gtgcgggac aagaacggt cggtattcc 
      cttcttcga gagccgctt tatctggac cgctgcgtc 
      atcgccgca gatccggcg tggcaaagc tacttcgaa 
      gtacaagaa ctcctagga aaggatact ttcattttt 
      aaggctgaa gacgaggag caaacgaaa atctggtat 
      agtcaatta cagtttcac gcccaagga atgggagcc 
      tggaggaaa cggcgtaat gccctagcc aatataatg 
      ataaatggc atgggctta aggtcctaa
    mRNA sequence
    • >TC003783 RA
      taataacag taaaaaatt aaaacttgt cacaattat 
      tgtgatagc aggagcagt gaggcataa caaatgaac 
      aaaatgtag gtgtctacc tgataataa ccgagtttt 
      ttgttacag gaaagtgaa actggaaga ggtagaaaa 
      ccattacaa acaaagtga tgccaaagc ggacacatt 
      ctcatggct gcagtggtt agccatgca atgcgacga 
      tttccaacg actcagcct gatgccgtg tattcttac 
      ggtctaagg cccgaagga tctcgcaaa ccctggtct 
      ccatccgaa gacggagag agcgctcta tcccgctcg 
      atgcgttat gccgacccc tggctctac ggcagcgtg 
      cgcgcacca gctggccta gtcctcgcc ccagcgttc 
      gtcctctgc acctgctcc gactacgtc aacggcacc 
      aaacgctcc tccaagaag gccccaacg acatgcaaa 
      aagtgcaaa ggcacgcgc ctcccgctc tccccctcc 
      gagagcaag ttcgggacc gtgcgatgc taccccacc 
      tccaccacc tccacgccc gcgcgggcc ggcaccatg 
      cgggtgctg agctcctcc aggccctcc atcctgccg 
      gccgccgac ccctacgac ctcatgcgg cgctcgcgc 
      ctcgcctcg cgctccgcc tcccccaag ggggcccct 
      cgggggcgg cgctccatc ctcgagtgc gacatcaac 
      ccctacgag ctcatgacg gccgagagc tcccccagc 
      atcacgctg gtcaacggc cagaggatc agggtgcgg 
      cccccgacg gcggaccag gagtacgag gacgtgcag 
      gtgaagaga ccggcccca aaagtgccc ccgaacaaa 
      aagccaaac gaaaaggaa tctgagtgc aaccggttc 
      aagtctata ttaaagaaa ccaacaact ttcagtgac 
      acggattca aacagccgt gagcgcagc ccctcgccc 
      gcgcacaaa agtgggtca cacttttac ctgccaatg 
      cccggcaac acacccagg aaaaaagtg cagtttttg 
      gttgaaaat gaacttgaa gtggaccaa aatcgaaag 
      gaggaggag gcgtcggaa gacgccgaa atgtcaaac 
      gaggaagga caagaaacc tccgatcaa aaagtctgc 
      aagttgcga cggagcgtc agcgaacgg ttcaatcat 
      ggctcgaag aaagttgta ttccatgat acaatggca 
      ttacgttca cgtccctcc cggagcgaa ctgagacta 
      gacttaggc gaggaagat ctcgagcga ttagacggc 
      ctgccatcg aaaaccagg cgaaaaagc cccacaaat 
      ccacggcca agtcaaccg cccccgccg ccgccgccc 
      gcctcgccc ccggttgaa aaacccaaa cccacatcg 
      aaatgtgcg aaaccagcg aaggtcgca cctcaagaa 
      gaaccaaat gtgaaaaca caaaaagct aagaaggtt 
      gcagtcaaa gaaccaatg gaaaagcaa gaagttgga 
      aatgtggac gagattaag ggaaaagtt gaggagaaa 
      acgcaggaa gttgcggtt ccaccgtct gttcctcct 
      cggaaaagg agcaacaat aaacaaccc cacgtgaat 
      ataattcag acgtctgcg agtgttcag tccgtccat 
      gttgagtca ccaagtgtt cattctgtt caaattgag 
      tcatcggct ttgataagt gaacccgca aacaaaacg 
      gtggttcaa attaacggc tcgccgacg gttcacaaa 
      ttgcagatt aagaacgaa ttcgcagat gtcgtgaac 
      gttcaaacg gtttccgtg cttgataat ccgcaaagg 
      aagacatcc atcatgata acaggtgac gattgttat 
      tcaaccgta aacgttaat gatgacgtt ccactttat 
      caatcttcg gtcgttgtg aacgatacg gcgaacgag 
      aaaactgta attcaaaat accaaaaag agcagttcc 
      gtttacatc accggtaat ttcgccgcg tctgaaagc 
      agcgaatgc tcgaaagag cccaaagcc aatgaatcg 
      aacaaaaac ggaagccat aagtacgac gattgcgaa 
      ttaatcgaa actgcacaa ataacgcac aaacaggag 
      aataatatc ctcttggac acgttcgac caaaccaat 
      attccctcc tcggagctg ctcaaggag ctgctcaaa 
      gaccccgtt gaagccgtg cggcggaat ttagtccct 
      catgtctgt ggtaaatca gacgtgtca aggagacca 
      aggcccaag aagttcgtt tttgaagaa acgcatctg 
      gaagcgagt gataacacg aacggtatc aacaacttc 
      atcgaagag tcgctcctc agaatgaag aatttggat 
      ttgaatgat gatctaaac tcggaacat agttcgtcg 
      actcagtac gagctcatg gacgccagt tctgagtgt 
      tatacagat cacagtaat cgtagctcg gttactgag 
      gaagaattg gcaaatagg acgaagttt tatgagttg 
      ttggctgac tcggcggtt gttgaagtc tcagaaagt 
      gaggatcat cattacgaa agtattaag aataattcg 
      gacccgatt tatgaagaa attgagatt ccgccgcca 
      ctgccggca aacccccca ccgttgagt cttctagac 
      gatctgcat ctggacaag gaatacacc acgaggtcc 
      atattcgag ggagcatcg aaatacgac attttaagt 
      tacttggtc gacgcaaaa gagcgcgga atcgtccaa 
      gaagacaat tactcgtac aatttcgcc aacaatgga 
      atttcagtt gacgatgat accaaagaa acgtacaac 
      agagtctgc catatgagc actgtgaac gattccagt 
      gaagacaac agtctgatc atgccgagt aatttaatc 
      gacgacaaa gtaccattc caaaaaacg gcagaagtc 
      gagagaaac gactccggc gttggttcc gaaaccagc 
      aagagttcc ctcagcaaa taccggatg aagcaagaa 
      acggccaca acttgcgaa gactgcgag gcagttctt 
      gagtcgtct caagacacc gagcccttg atttgccgc 
      aagtgcttg aaaaagcgc tcggaacgg aaggaaatc 
      atcaccgaa atcgtggag actgaggag aagtacagt 
      cgcgatttg cagattatt ttggaggag ttttaccaa 
      ccgatgtta gtggctggg ttattgacg ccggagcaa 
      ctatcggcg attttcctc aacgtggag gaacttctg 
      gaaaacagt caaagtctg gcggaacgg ttgcgagac 
      gctgtggaa attgcagtc gaacaagga gatgaagat 
      ctcctaact gttaacatc gggaagatt ttcctggag 
      gcggccccc atgttgcac ggatttgag acctattgc 
      gtccggcaa ggcgcagca agtctgctt ttagctagt 
      ctagagaag gaaaaagag ctcctcagg atattcctc 
      cgggtgtcg caaatggag aacacgatg ctgagaagg 
      atgaatttg aattcgttc ctcatggta cctgtccaa 
      cgtgtcact aaatacccg ctgttgttg gcgcggttg 
      tataaggtt acgccggtg caccacgag acgagggaa 
      cagttgaag gaagcgcaa cacaagatt gagttacat 
      ctgaatcac atgaactcc gaaacgaag gacgtgccg 
      agtaagctg tggaggagg attgggagt agttccggg 
      aggaggtca agcaccgaa atggatttg gttaatata 
      aagttgagg aagattgcg gttgatgtg ttggagtgg 
      aatcatgat gaggccaag tttgcgttg gaggggaaa 
      ttgctgttt acgcaaccg acggataat aattggagg 
      aaggggagg acgatcaag ctgacgccg gttaatgcc 
      cttcttgtg atcaatggc aagccatca atgaatcga 
      ccggaacga accgacgag aatgggttg atattttca 
      cggcattct gcggcccgt gaagcggct cttcttctg 
      gtgcgggac aagaacggt cggtattcc cttcttcga 
      gagccgctt tatctggac cgctgcgtc atcgccgca 
      gatccggcg tggcaaagc tacttcgaa gtacaagaa 
      ctcctagga aaggatact ttcattttt aaggctgaa 
      gacgaggag caaacgaaa atctggtat agtcaatta 
      cagtttcac gcccaagga atgggagcc tggaggaaa 
      cggcgtaat gccctagcc aatataatg ataaatggc 
      atgggctta aggtcctaa tcgacgaac ttactgaaa 
      ataaagatt taatgagct ttaagtcat tacaactcg 
      aatgtttcc tgtaatttg tacttgatc gatataggt 
      aattttatt atttctttt tgtaaatat ttagtaatt 
      tagctgtga tattttccg gatgaggtt tttgggatt 
      caatttatt ttacaataa taatatgct tgacgacca 
      gtttatggt tattgtaac tttatatga ctttatatt 
      aatcattgt aaaaacatg taattaaat aaaaataaa 
      taaataatt taaaatatg