Status
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Gene information
General information
TC031050
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003776,TC003777,TC003778
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid21: 1515287..1518801
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031050
    iBeetle screen iB_00611
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    iBeetle screen iB_00612
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    remarks: animals lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031050 RA
      MEKFSTLLF FYLVVSAHT ELICYDCDP TSPGKCISP 
      SQVVVKTTI CDEVNGHEQ VGTPECFTV EYHTDDPTS 
      REKSGIYRG CFAKTDEAT CVHLNQTYA GKVTWCKSC 
      AKNKCLKSN KLVEQFCIK TPPKMTKLA AVFLLHLLV 
      LAHSELICY DCDPTSPGK CISPSLDNA RMTNCDASN 
      ENEGISIPT IPECFTLQY YTNNRSRNG IYRGCTSKV 
      VNKPTCDHL NETLNGKVT WCRTCSETR CNFLKTTMA 
      QLGSIISII LTCFLVNGA KSATFENVT VLLTTEWNK 
      LFYLGRTPM EKVYVASSN TIAEFVNLQ PLEIVWIRN 
      EHIPYLREN SLSNLPNIV QISVVSSGV KKIAPGTFQ 
      NDPKLDVIN LESNQIEEV PNGVFTGLQ LKALYLSNN 
      QISRMGSAA FSNMTMTVL KMDNNKISR WRSEVFMGT 
      PIGTLSLAN NLIEEIPAN AFSYGFQFK RINVPLAAW 
      HVVLSQNRI RKIHPQAFS LVNGFGYLS LADNYLKDL 
      PKDLFRYVK SINTLDLTN NQISDVGGV FDDININQL 
      ILINNTLNC FPDEVFDIS RINFVFLDG NPVTCGCVA 
      MWKDIQLRN NIWVVFNMK DSQIKVNLY KTSRYQILN 
      MVRLLCLVV VLFVPQIQG ATYENVTIL LTTRHTVYF 
      INDTVAPTK PVRIRYDNT LAEYVNNQP WEIVKIQDQ 
      NVPNLPENA VSRLPNITE VSIISSGLE TIEPGAFGD 
      LPAMEYLYI NENPLKVIP NGVFAHLSR LSSLHLENN 
      SIETLGDKV FNELPHLGH VFLDGNHIS TWNSDWFIG 
      SPVESVSMA FNSIEELPS GMFDYFLKF QDMRSSGLW 
      YLVLESNKV RKIDPDAFR GLKELLYLN LKNNSIEEL 
      PTGLFHTIK YINNLELSD NVIKDINPS IFRNTSLTY 
      VKLGNNKLT CLPLELMNV TKWYNMNID GNPVTCGCV 
      KSWKILQND SYAENTVSL GNIEKNCTE
    CDS sequence
    • >TC031050 RA
      atggagaag ttctcaact ttgttgttc ttttatctc 
      gtcgtgtcg gctcacacg gaattaatt tgttacgat 
      tgtgatcct acgagtcct ggaaaatgt attagtccg 
      agtcaggtc gttgtaaaa acgaccatt tgtgatgaa 
      gtaaatgga catgaacaa gttggtaca cctgaatgt 
      ttcactgtt gagtatcac acagatgat ccaacaagc 
      agggaaaaa agtggtatc tatagaggg tgttttgca 
      aaaactgat gaagcaact tgtgttcat ttaaaccag 
      acttatgcg ggaaaagtc acttggtgt aaaagttgt 
      gctaaaaac aaatgtcta aagtcaaat aagttagtc 
      gaacagttt tgcattaaa acacctcca aaaatgacg 
      aagttagca gcggtattt ttgttgcat ctacttgtg 
      ctagctcat tcggagttg atttgctac gattgtgat 
      cctacaagt cctggcaaa tgtataagt cccagtctg 
      gataacgca agaatgact aactgtgat gcttcaaat 
      gaaaacgaa ggaatttcg attcctaca attcctgag 
      tgtttcact cttcaatat tacactaac aataggtct 
      aggaatggg atttataga ggttgcact tcgaaagtc 
      gtcaataaa ccgacgtgt gatcacttg aatgaaaca 
      ctaaacggt aaagtgact tggtgtaga acttgcagt 
      gaaaccaga tgtaacttt cttaaaaca acaatggct 
      cagctcggt tcaattatt tcgataatt ctaacgtgt 
      tttcttgta aatggtgcc aaaagtgca acttttgaa 
      aatgtgacc gttttatta acaacagaa tggaacaaa 
      ctcttctac ctaggacgc actccaatg gaaaaagtt 
      tacgtagcg tcgtctaac acgatcgca gagtttgtt 
      aatttgcaa cccttggaa atcgtctgg attcgtaac 
      gaacatatc ccatacctt cgcgaaaat tccctttcc 
      aatttgcca aatatcgtc caaatttca gtggtgtca 
      agtggggtc aaaaaaatc gcccctggg acgtttcaa 
      aatgaccca aaattggac gttatcaac ctggaaagc 
      aaccagatt gaggaagta ccaaatggg gtgtttaca 
      ggtctccag ttaaaagcc ttgtatttg agtaataac 
      caaatttca agaatgggg agtgcggcg tttagtaac 
      atgacaatg actgtgctc aagatggac aataacaag 
      atttcgagg tggaggagt gaggttttt atgggcact 
      cccattggt accttatct ctagccaat aatctaatc 
      gaggaaata ccggcaaat gcattctcg tacggtttc 
      cagtttaaa aggattaat gttccgttg gcagcctgg 
      cacgtggtt ttgtcccaa aaccggatc cggaagatt 
      caccctcaa gccttcagt ctggtcaat ggttttggt 
      tatttgagt ctcgcggat aattatttg aaggatcta 
      ccgaaggat ttgttccgc tacgtcaaa agtatcaac 
      actttggac ttgaccaat aaccaaatt tcagacgtg 
      ggaggagtg tttgatgac attaatata aaccagttg 
      attctaatt aacaacact ttgaattgt ttcccggat 
      gaggtgttt gacatttca aggattaat ttcgtgttt 
      ctcgacggc aatcctgtg acttgtggg tgtgtagca 
      atgtggaaa gacatccaa ttaaggaac aacatttgg 
      gttgtcttc aatatgaaa gactcgcaa atcaaggtt 
      aatttgtat aagaccagt cgttaccag attctcaat 
      atggtaagg cttttgtgt cttgtggtt gtgctgttt 
      gtgccccaa attcaaggc gccacttac gaaaatgtt 
      acaattttg ttaactacg agacataca gtgtacttc 
      attaatgat acagtagca ccgaccaaa cctgttcga 
      attcgttac gacaacacc cttgccgag tatgtcaac 
      aaccagccg tgggaaatc gtcaaaatt caggaccaa 
      aatgtcccg aatttacca gaaaatgca gtctcacgt 
      ttacccaat attacagaa gttagtatt atttcaagt 
      ggtttggaa acaatcgag cctggggcg tttggggat 
      ttaccagcg atggaatat ctctacatt aatgaaaat 
      cctttaaaa gtgattcca aatggtgtt tttgcacac 
      ttgtcacgt ttgagttcg cttcatttg gaaaataat 
      tcgatagag accttgggt gataaagtt ttcaacgaa 
      cttccacat ttgggacat gttttcctc gacggtaac 
      cacatttcg acttggaat agtgactgg tttattggg 
      tcgcctgtg gagtccgtt tcaatggcc tttaactcg 
      attgaggag ttacctagt ggcatgttt gattacttc 
      ctcaaattt caggacatg cgaagctct gggctttgg 
      tacttggtt ttggaaagt aacaaagtg cggaaaatc 
      gatccggac gcgtttcga gggttaaaa gagttgttg 
      tatttgaac ctcaaaaat aactcaatt gaggagctc 
      ccgactggt ctttttcac acaatcaag tacataaat 
      aacctcgaa ttgagtgat aatgtgata aaagacata 
      aacccctca atttttcgt aatacatcg ctcacctac 
      gtcaaattg gggaacaat aaattgacg tgtttgcct 
      ctggaactt atgaacgtc accaaatgg tataacatg 
      aatattgat ggaaatccg gtcacttgt ggctgcgtc 
      aagagttgg aagattttg cagaacgat tcttacgcg 
      gaaaatacg gtgtcactt ggaaatatc gaaaaaaat 
      tgtaccgaa taa
    mRNA sequence
    • >TC031050 RA
      ggtaataat tgaaatgaa aagtttttt aataactgt 
      tatcaaagt ttcaaatat tgcgttttc agtttacga 
      cacgatatg gagaagttc tcaactttg ttgttcttt 
      tatctcgtc gtgtcggct cacacggaa ttaatttgt 
      tacgattgt gatcctacg agtcctgga aaatgtatt 
      agtccgagt caggtcgtt gtaaaaacg accatttgt 
      gatgaagta aatggacat gaacaagtt ggtacacct 
      gaatgtttc actgttgag tatcacaca gatgatcca 
      acaagcagg gaaaaaagt ggtatctat agagggtgt 
      tttgcaaaa actgatgaa gcaacttgt gttcattta 
      aaccagact tatgcggga aaagtcact tggtgtaaa 
      agttgtgct aaaaacaaa tgtctaaag tcaaataag 
      ttagtcgaa cagttttgc attaaaaca cctccaaaa 
      atgacgaag ttagcagcg gtatttttg ttgcatcta 
      cttgtgcta gctcattcg gagttgatt tgctacgat 
      tgtgatcct acaagtcct ggcaaatgt ataagtccc 
      agtctggat aacgcaaga atgactaac tgtgatgct 
      tcaaatgaa aacgaagga atttcgatt cctacaatt 
      cctgagtgt ttcactctt caatattac actaacaat 
      aggtctagg aatgggatt tatagaggt tgcacttcg 
      aaagtcgtc aataaaccg acgtgtgat cacttgaat 
      gaaacacta aacggtaaa gtgacttgg tgtagaact 
      tgcagtgaa accagatgt aactttctt aaaacaaca 
      atggctcag ctcggttca attatttcg ataattcta 
      acgtgtttt cttgtaaat ggtgccaaa agtgcaact 
      tttgaaaat gtgaccgtt ttattaaca acagaatgg 
      aacaaactc ttctaccta ggacgcact ccaatggaa 
      aaagtttac gtagcgtcg tctaacacg atcgcagag 
      tttgttaat ttgcaaccc ttggaaatc gtctggatt 
      cgtaacgaa catatccca taccttcgc gaaaattcc 
      ctttccaat ttgccaaat atcgtccaa atttcagtg 
      gtgtcaagt ggggtcaaa aaaatcgcc cctgggacg 
      tttcaaaat gacccaaaa ttggacgtt atcaacctg 
      gaaagcaac cagattgag gaagtacca aatggggtg 
      tttacaggt ctccagtta aaagccttg tatttgagt 
      aataaccaa atttcaaga atggggagt gcggcgttt 
      agtaacatg acaatgact gtgctcaag atggacaat 
      aacaagatt tcgaggtgg aggagtgag gtttttatg 
      ggcactccc attggtacc ttatctcta gccaataat 
      ctaatcgag gaaataccg gcaaatgca ttctcgtac 
      ggtttccag tttaaaagg attaatgtt ccgttggca 
      gcctggcac gtggttttg tcccaaaac cggatccgg 
      aagattcac cctcaagcc ttcagtctg gtcaatggt 
      tttggttat ttgagtctc gcggataat tatttgaag 
      gatctaccg aaggatttg ttccgctac gtcaaaagt 
      atcaacact ttggacttg accaataac caaatttca 
      gacgtggga ggagtgttt gatgacatt aatataaac 
      cagttgatt ctaattaac aacactttg aattgtttc 
      ccggatgag gtgtttgac atttcaagg attaatttc 
      gtgtttctc gacggcaat cctgtgact tgtgggtgt 
      gtagcaatg tggaaagac atccaatta aggaacaac 
      atttgggtt gtcttcaat atgaaagac tcgcaaatc 
      aaggttaat ttgtataag accagtcgt taccagatt 
      ctcaatatg gtaaggctt ttgtgtctt gtggttgtg 
      ctgtttgtg ccccaaatt caaggcgcc acttacgaa 
      aatgttaca attttgtta actacgaga catacagtg 
      tacttcatt aatgataca gtagcaccg accaaacct 
      gttcgaatt cgttacgac aacaccctt gccgagtat 
      gtcaacaac cagccgtgg gaaatcgtc aaaattcag 
      gaccaaaat gtcccgaat ttaccagaa aatgcagtc 
      tcacgttta cccaatatt acagaagtt agtattatt 
      tcaagtggt ttggaaaca atcgagcct ggggcgttt 
      ggggattta ccagcgatg gaatatctc tacattaat 
      gaaaatcct ttaaaagtg attccaaat ggtgttttt 
      gcacacttg tcacgtttg agttcgctt catttggaa 
      aataattcg atagagacc ttgggtgat aaagttttc 
      aacgaactt ccacatttg ggacatgtt ttcctcgac 
      ggtaaccac atttcgact tggaatagt gactggttt 
      attgggtcg cctgtggag tccgtttca atggccttt 
      aactcgatt gaggagtta cctagtggc atgtttgat 
      tacttcctc aaatttcag gacatgcga agctctggg 
      ctttggtac ttggttttg gaaagtaac aaagtgcgg 
      aaaatcgat ccggacgcg tttcgaggg ttaaaagag 
      ttgttgtat ttgaacctc aaaaataac tcaattgag 
      gagctcccg actggtctt tttcacaca atcaagtac 
      ataaataac ctcgaattg agtgataat gtgataaaa 
      gacataaac ccctcaatt tttcgtaat acatcgctc 
      acctacgtc aaattgggg aacaataaa ttgacgtgt 
      ttgcctctg gaacttatg aacgtcacc aaatggtat 
      aacatgaat attgatgga aatccggtc acttgtggc 
      tgcgtcaag agttggaag attttgcag aacgattct 
      tacgcggaa aatacggtg tcacttgga aatatcgaa 
      aaaaattgt accgaataa atttttgtt gtatttttt 
      ttaataaaa attcttctt caca