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Gene information
General information
TC003759 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18198167..18204036
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003759
    iBeetle screen iB_00610

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Adult morphology
    motility decreased - (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003759 RA
      MSEKKQLQP IKVFVRPRP LMPRDDKLK VSKVIRHTE 
      ARELVIKSG PIDKKYNFD HVFNENASQ KEVYEAVVV 
      PFISSIPEG YTCTIFAYG QSGTGKTHT FFGEHEVDI 
      DSDFENDVS IGLILRAAL QLFSELGKL NARTDYTVR 
      FSFIEIVNE EVRDLLDDS NHLRVYDDP LKKGSVCLK 
      NMTKCTVFN VHDVNDCII AGNKLVSKN SNGGSSHVI 
      ITFTVDTRE RFSNGTECI KTGRLNFVE LAGSQNMTR 
      STQQRAKDF ALTNKSLIT FGNVIKAIE QKLQYVPYR 
      ESKLTRILQ DSIGGNAKT CMIATFSSS SLHIDETVN 
      TLEFAKIAK CVVNCPQIN VCRKQTDLS RELQDEVER 
      LRKLVTAAR ESQGFYVSA ENYNKMTQE QKDNDAKII 
      QLLRQIREL EEHKRTLQI QNDNKNDSF LKTKLFVED 
      AEKQLKAKQ NETKQSESR LNALAARNE EMIRNANRA 
      LDLCEKRLK EEAIYYRKY QEKCEANKT NTTLVKEIN 
      QTTSSGMKD IQQATYAYV NQQKNRFKY IAEKQRIVR 
      QSFMDLAST IGTFKQLDA QESVKNESD DVTELIIKN 
      IHDNTAEKL KSQKELKDV VDEEKKRFA EINEKVKQM 
      AEETVEEVK NNVIIFGNI QSQIHDNVL SGN
    CDS sequence
    • >TC003759 RA
      atgagtgaa aagaagcag ttacagccg attaaggtg 
      tttgtaagg cctaggcct ctgatgcca agggatgat 
      aaactgaaa gtatcaaag gtaatccgg catacagag 
      gcacgagaa ttggttatt aaaagtgga ccaattgat 
      aaaaaatac aactttgat cacgttttc aacgagaat 
      gcttcacaa aaggaggtt tacgaggct gttgtggtc 
      cccttcatt agtagcatt cctgaaggt tacacctgt 
      actattttt gcctatgga caatcaggc actgggaag 
      acacacacg ttttttggg gagcatgaa gtggacatt 
      gattccgac ttcgaaaac gatgtgtca attgggcta 
      attctgcgc gctgctctg caacttttt tccgaattg 
      ggtaaacta aatgcaaga actgattac acagtgaga 
      ttttctttt atcgaaatc gtcaacgaa gaagttcgt 
      gatttactt gatgacagt aatcatttg agagtttat 
      gatgaccca ttgaagaaa ggttcagtt tgcttgaag 
      aatatgaca aagtgtacg gtttttaat gttcatgac 
      gtaaatgat tgtattatt gctggtaac aagttagta 
      tcgaagaac tcaaatggt ggaagttct cacgttata 
      atcaccttc acggtggac accagagaa agattctct 
      aatggcaca gagtgtata aaaaccggt cgtttaaat 
      ttcgttgag ttagccggt agtcagaat atgacacgc 
      tctacacaa caaagagcc aaggatttt gctttgacc 
      aataagtcg ctaattaca tttggaaac gtcattaaa 
      gctatagag caaaagcta caatacgtt ccgtatcgc 
      gaatcaaaa ttaactcga attctgcaa gatagtatc 
      ggcggtaac gccaaaact tgtatgatt gcaacattt 
      tcatccagc agtcttcat attgacgaa accgttaac 
      actttagaa tttgcaaaa atcgcaaaa tgtgttgtt 
      aactgtccc caaattaac gtctgccgc aaacaaaca 
      gacctgtca cgagaactg caagacgaa gtcgaacga 
      ttgagaaaa ttagtgact gctgcaaga gaaagccaa 
      ggcttctac gtcagtgca gaaaactac aacaaaatg 
      acacaagaa caaaaggat aatgatgct aaaataatc 
      caactactg cgtcaaatt cgcgaattg gaagagcac 
      aaaagaaca ctccaaatt caaaatgac aacaaaaac 
      gattcgttt ctcaaaact aaactattc gtcgaagat 
      gcggaaaag cagttaaaa gccaaacag aacgaaaca 
      aaacagagt gaatcgcgc ttgaacgct ttagcggcg 
      cgaaatgaa gaaatgatt cgcaatgca aaccgagcc 
      cttgatctt tgcgaaaag agacttaaa gaagaagca 
      atttattac cgaaagtat caagagaag tgtgaagct 
      aataaaacc aacactacc ttggtgaag gaaatcaat 
      caaaccacg agcagtggt atgaaagat atccaacaa 
      gcgacctac gcttatgtt aatcaacaa aagaatcgt 
      tttaaatat atagctgaa aaacaacga attgttcga 
      caatccttc atggatctg gcgtccact attggaact 
      tttaaacaa ttagatgcc caagaaagt gtcaaaaac 
      gaatccgat gatgtaaca gagttaata atcaaaaac 
      atccatgac aacactgca gagaaatta aaatctcaa 
      aaggagctc aaagatgtg gtggatgag gaaaagaag 
      cgctttgct gaaatcaat gaaaaggtt aaacagatg 
      gcagaagaa actgttgag gaggtaaaa aataatgtg 
      attatattt ggcaatatt cagtctcag attcatgat 
      aacgtactt tcaggaaat taa
    mRNA sequence
    • >TC003759 RA
      ttataacag aaaaaattc aaactagag aagaggaag 
      acggttaaa aagttgttt agttgtgga agacctgaa 
      aaatgagtg aaaagaagc agttacagc cgattaagg 
      tgtttgtaa ggcctaggc ctctgatgc caagggatg 
      ataaactga aagtatcaa aggtaatcc ggcatacag 
      aggcacgag aattggtta ttaaaagtg gaccaattg 
      ataaaaaat acaactttg atcacgttt tcaacgaga 
      atgcttcac aaaaggagg tttacgagg ctgttgtgg 
      tccccttca ttagtagca ttcctgaag gttacacct 
      gtactattt ttgcctatg gacaatcag gcactggga 
      agacacaca cgttttttg gggagcatg aagtggaca 
      ttgattccg acttcgaaa acgatgtgt caattgggc 
      taattctgc gcgctgctc tgcaacttt tttccgaat 
      tgggtaaac taaatgcaa gaactgatt acacagtga 
      gattttctt ttatcgaaa tcgtcaacg aagaagttc 
      gtgatttac ttgatgaca gtaatcatt tgagagttt 
      atgatgacc cattgaaga aaggttcag tttgcttga 
      agaatatga caaagtgta cggttttta atgttcatg 
      acgtaaatg attgtatta ttgctggta acaagttag 
      tatcgaaga actcaaatg gtggaagtt ctcacgtta 
      taatcacct tcacggtgg acaccagag aaagattct 
      ctaatggca cagagtgta taaaaaccg gtcgtttaa 
      atttcgttg agttagccg gtagtcaga atatgacac 
      gctctacac aacaaagag ccaaggatt ttgctttga 
      ccaataagt cgctaatta catttggaa acgtcatta 
      aagctatag agcaaaagc tacaatacg ttccgtatc 
      gcgaatcaa aattaactc gaattctgc aagatagta 
      tcggcggta acgccaaaa cttgtatga ttgcaacat 
      tttcatcca gcagtcttc atattgacg aaaccgtta 
      acactttag aatttgcaa aaatcgcaa aatgtgttg 
      ttaactgtc cccaaatta acgtctgcc gcaaacaaa 
      cagacctgt cacgagaac tgcaagacg aagtcgaac 
      gattgagaa aattagtga ctgctgcaa gagaaagcc 
      aaggcttct acgtcagtg cagaaaact acaacaaaa 
      tgacacaag aacaaaagg ataatgatg ctaaaataa 
      tccaactac tgcgtcaaa ttcgcgaat tggaagagc 
      acaaaagaa cactccaaa ttcaaaatg acaacaaaa 
      acgattcgt ttctcaaaa ctaaactat tcgtcgaag 
      atgcggaaa agcagttaa aagccaaac agaacgaaa 
      caaaacaga gtgaatcgc gcttgaacg ctttagcgg 
      cgcgaaatg aagaaatga ttcgcaatg caaaccgag 
      cccttgatc tttgcgaaa agagactta aagaagaag 
      caatttatt accgaaagt atcaagaga agtgtgaag 
      ctaataaaa ccaacacta ccttggtga aggaaatca 
      atcaaacca cgagcagtg gtatgaaag atatccaac 
      aagcgacct acgcttatg ttaatcaac aaaagaatc 
      gttttaaat atatagctg aaaaacaac gaattgttc 
      gacaatcct tcatggatc tggcgtcca ctattggaa 
      cttttaaac aattagatg cccaagaaa gtgtcaaaa 
      acgaatccg atgatgtaa cagagttaa taatcaaaa 
      acatccatg acaacactg cagagaaat taaaatctc 
      aaaaggagc tcaaagatg tggtggatg aggaaaaga 
      agcgctttg ctgaaatca atgaaaagg ttaaacaga 
      tggcagaag aaactgttg aggaggtaa aaaataatg 
      tgattatat ttggcaata ttcagtctc agattcatg 
      ataacgtac tttcaggaa attaatgtg ttggcaaca 
      gcctgaaat aaaaccaag gacttgaat aaaacatat 
      cttgtaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY