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Gene information
General information
TC003754 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 18381902..18412890
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003754
    iBeetle screen iB_00608

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003754 RA
      MLLPTNSTS VGFQQELYH SVTEKTKFF ASKCLNRSI 
      SPSGDRMND SFRGRFRFC VCTLPRKSN KKTTKWYVK 
      QPTWRLWGE EKADGALYT VYLKKVRYH RPTRSLSSS 
      HSDSDDEIS HLEWETVRV RFVKAGTLK KLVEALSTD 
      DGELETTYI NVFLATYRS FSTPREVLR LLLQRYEEL 
      LDAPPNGKI DQHEQHRKT LVSALHVWL DSYPEDFKD 
      PPNYTALNH LLHFCEEFL PATELHAKV QHRLERYSR 
      EAQADPLLV PPPAFALRA AAAAAGWKV YKLPDVPVR 
      HFAEQLTRM DVDLFKKLV PHQCLGAVW SRRDKSRSH 
      EAATVLATV NQFNAVSFR VISSILVEP GLRPIDRAQ 
      IITCWIDIA QELRLIKNF SSLKAIISG LQSNPIYRL 
      HRTWQSIPK EKIELFDEL AKIFSEDNN QWTQRELLM 
      REGTAKYAD TVGENDKQL QKIFQKQNH HTANISFGT 
      IPYLGTFLT DLTMIDTAI PDVIGDGLI NFDKRRKEF 
      EVLAQIKLL QGAANAYRF DADPAFDRW FDSILVLDD 
      REAYQLSCQ IEPGNNGSK LKKKASGHQ KNDSIASTS 
      SSNSSQFYC DIDSMPSSP HNSIDRKLS PSQMSNSSS 
      NSSLPSLDV SLNSSHSGG ASNKLQVPY VGNNTGTLS 
      PSHSQKSSP DFYIIKVTY ETDSVETDG IVLYKSIML 
      SNNERTPQV IRNAMYKLG LEGNPDRYT LAQVLPDKE 
      MVLPSNANV YYAVNPAYN LNFILRPKK SEESDTSMK 
      KGK
    CDS sequence
    • >TC003754 RA
      atgctgcta ccgaccaac tcgacgtca gtcggcttt 
      caacaagaa ctttatcac agtgtcacc gaaaaaact 
      aagtttttt gcctccaaa tgccttaac aggagtatt 
      agtcctagc ggtgataga atgaacgat tcgtttcgg 
      ggaaggttc aggttttgc gtttgtaca ctgccgagg 
      aaaagtaac aaaaagacg accaagtgg tatgtgaag 
      cagcccaca tggcgactg tggggcgag gaaaaagcc 
      gatggcgct ctctacacc gtctacctc aaaaaagtt 
      cgttaccat cgacccacg agaagtttg tcatcatca 
      cattcggat tcagatgac gaaatctcc catttggaa 
      tgggagacg gtccgtgtc cgtttcgtc aaagctggc 
      actttgaaa aagctcgtc gaagctctt tcaacggac 
      gatggtgag cttgaaacc acatatatt aatgtgttt 
      ttggccacc tatcggagc ttttctacg ccacgtgaa 
      gttctccga ctgttgctt cagcgctac gaggagctt 
      ttagatgcg ccacccaac gggaaaata gatcagcat 
      gagcagcac cggaagact ctcgtttcg gcgctgcac 
      gtctggttg gatagttac ccggaggat ttcaaggat 
      cctccgaat tataccgca cttaaccat ttgcttcat 
      ttctgcgag gagtttctt cctgcgact gaattacac 
      gccaaagtg caacacagg ttggagagg tatagcagg 
      gaagctcaa gccgatcct ttgctggtg ccaccccca 
      gcgtttgct ttgagggcg gcggcggcg gcggccgga 
      tggaaagtc tataaactt ccggatgta ccggtgcga 
      cattttgcc gaacagctt acgaggatg gatgtggac 
      cttttcaag aaactcgtc cctcaccaa tgcctaggc 
      gccgtatgg tcccgtcgc gacaaaagc cgctcccat 
      gaggcggcc accgtctta gccaccgtc aaccaattc 
      aacgccgtt tcattccgc gtcatttcg tcaattcta 
      gtcgaaccc ggtctcaga cccatagac agggcacaa 
      ataatcaca tgctggata gacatagca caagaactg 
      cgcctcata aaaaacttc tcaagtcta aaagccatc 
      atttcgggc ttacagagc aacccaatc tatcgatta 
      caccgcact tggcagtcc atacctaag gaaaaaatc 
      gaactcttc gacgaactc gccaaaatc ttcagcgag 
      gacaacaac cagtggaca caacgggaa ttgctgatg 
      cgcgaaggc acagccaaa tatgccgac acagtcggc 
      gaaaacgac aaacagctc cagaaaatc ttccagaaa 
      cagaaccac cacacggcg aatatctcc ttcgggacg 
      attccctat ttggggacg tttttgacc gacttgacc 
      atgattgac acagcgatt cccgacgtt attggcgat 
      ggtttaatc aactttgat aaaaggcgc aaagagttt 
      gaggttttg gctcagatt aagttgctc caaggggct 
      gccaacgcg taccgtttc gatgcagat ccggctttt 
      gaccgttgg ttcgattcg attttggtg ttggacgat 
      agagaggcg taccagctt agttgtcaa atcgagccg 
      gggaataac ggctcaaaa ttgaagaaa aaagcgtca 
      gggcatcag aaaaacgac tcgattgcg agcacttcg 
      agtagcaac agttcgcag ttttattgc gatattgat 
      agtatgccc agttcgccg cataatagc atcgatcgt 
      aaactgagt ccgtcgcaa atgtcgaat tcgagtagt 
      aattcgtcg cttccgtcg ttggacgtt tcgttgaat 
      agttcgcac tcgggtggt gcgagtaat aaattgcaa 
      gttccgtac gtggggaac aatacgggg acgttgagt 
      ccgagtcat tcgcagaag agtagtcca gatttttat 
      attattaaa gttacttat gaaacggat agtgtggag 
      actgatggg attgtcttg tataagagt attatgttg 
      agtaataat gagaggacg ccgcaggtt atcaggaat 
      gcgatgtac aagttgggg ttggaaggg aatcctgat 
      agatatacg ctagcgcaa gttttgccc gataaagag 
      atggtcctt ccatcaaat gctaatgtc tactacgcc 
      gtgaatccc gcctacaac ttgaatttt attctgcgt 
      ccgaagaaa tccgaagag tctgacact agtatgaag 
      aaagggaag tga
    mRNA sequence
    • >TC003754 RA
      gcaggtgct atatttata gtaagtccg agggcgctt 
      tgcgttgcg cttgcgtta aaaatgtca attaaacga 
      cacgctacg ctcgcagag ctcgcagga agcaatgat 
      attcattat actgtcggg ggacgtttg gagtagtgg 
      atcaaagcg cgtcggtgc ggtcgtcct tttatctat 
      gcactcctt cagattgcc gccgatttc tcgcattat 
      cgattcgag gatttaatc caatctccg tgattctct 
      tggattatt caacggatc gtcaatagt tatgttgct 
      tttcggttg atttcacga aaaaaaata acatgctgc 
      taccgacca actcgacgt cagtcggct ttcaacaag 
      aactttatc acagtgtca ccgaaaaaa ctaagtttt 
      ttgcctcca aatgcctta acaggagta ttagtccta 
      gcggtgata gaatgaacg attcgtttc ggggaaggt 
      tcaggtttt gcgtttgta cactgccga ggaaaagta 
      acaaaaaga cgaccaagt ggtatgtga agcagccca 
      catggcgac tgtggggcg aggaaaaag ccgatggcg 
      ctctctaca ccgtctacc tcaaaaaag ttcgttacc 
      atcgaccca cgagaagtt tgtcatcat cacattcgg 
      attcagatg acgaaatct cccatttgg aatgggaga 
      cggtccgtg tccgtttcg tcaaagctg gcactttga 
      aaaagctcg tcgaagctc tttcaacgg acgatggtg 
      agcttgaaa ccacatata ttaatgtgt ttttggcca 
      cctatcgga gcttttcta cgccacgtg aagttctcc 
      gactgttgc ttcagcgct acgaggagc ttttagatg 
      cgccaccca acgggaaaa tagatcagc atgagcagc 
      accggaaga ctctcgttt cggcgctgc acgtctggt 
      tggatagtt acccggagg atttcaagg atcctccga 
      attataccg cacttaacc atttgcttc atttctgcg 
      aggagtttc ttcctgcga ctgaattac acgccaaag 
      tgcaacaca ggttggaga ggtatagca gggaagctc 
      aagccgatc ctttgctgg tgccacccc cagcgtttg 
      ctttgaggg cggcggcgg cggcggccg gatggaaag 
      tctataaac ttccggatg taccggtgc gacattttg 
      ccgaacagc ttacgagga tggatgtgg accttttca 
      agaaactcg tccctcacc aatgcctag gcgccgtat 
      ggtcccgtc gcgacaaaa gccgctccc atgaggcgg 
      ccaccgtct tagccaccg tcaaccaat tcaacgccg 
      tttcattcc gcgtcattt cgtcaattc tagtcgaac 
      ccggtctca gacccatag acagggcac aaataatca 
      catgctgga tagacatag cacaagaac tgcgcctca 
      taaaaaact tctcaagtc taaaagcca tcatttcgg 
      gcttacaga gcaacccaa tctatcgat tacaccgca 
      cttggcagt ccataccta aggaaaaaa tcgaactct 
      tcgacgaac tcgccaaaa tcttcagcg aggacaaca 
      accagtgga cacaacggg aattgctga tgcgcgaag 
      gcacagcca aatatgccg acacagtcg gcgaaaacg 
      acaaacagc tccagaaaa tcttccaga aacagaacc 
      accacacgg cgaatatct ccttcggga cgattccct 
      atttgggga cgtttttga ccgacttga ccatgattg 
      acacagcga ttcccgacg ttattggcg atggtttaa 
      tcaactttg ataaaaggc gcaaagagt ttgaggttt 
      tggctcaga ttaagttgc tccaagggg ctgccaacg 
      cgtaccgtt tcgatgcag atccggctt ttgaccgtt 
      ggttcgatt cgattttgg tgttggacg atagagagg 
      cgtaccagc ttagttgtc aaatcgagc cggggaata 
      acggctcaa aattgaaga aaaaagcgt cagggcatc 
      agaaaaacg actcgattg cgagcactt cgagtagca 
      acagttcgc agttttatt gcgatattg atagtatgc 
      ccagttcgc cgcataata gcatcgatc gtaaactga 
      gtccgtcgc aaatgtcga attcgagta gtaattcgt 
      cgcttccgt cgttggacg tttcgttga atagttcgc 
      actcgggtg gtgcgagta ataaattgc aagttccgt 
      acgtgggga acaatacgg ggacgttga gtccgagtc 
      attcgcaga agagtagtc cagattttt atattatta 
      aagttactt atgaaacgg atagtgtgg agactgatg 
      ggattgtct tgtataaga gtattatgt tgagtaata 
      atgagagga cgccgcagg ttatcagga atgcgatgt 
      acaagttgg ggttggaag ggaatcctg atagatata 
      cgctagcgc aagttttgc ccgataaag agatggtcc 
      ttccatcaa atgctaatg tctactacg ccgtgaatc 
      ccgcctaca acttgaatt ttattctgc gtccgaaga 
      aatccgaag agtctgaca ctagtatga agaaaggga 
      agtgaagcc gctacataa taggtgcaa tttaattta 
      attaaatgg taaatgaaa tgagctgtt tttgacaaa 
      taacggaac aaataacaa ctgtgataa aaagcacaa 
      atactctct ttttacatc acattttac gtttttatt 
      tttatttat ttattgcga tgaggattt gaaatgtgc 
      tgaagtaac catatggca tggattgtt ttatttatt 
      tcacttttt taaattaat ttaaagctt tactttttg 
      tgtaattaa tgtaaatta ataatgggc taagtattg 
      taaatgtga acaaatgta atttattgt acttattaa 
      tgtagagtg tgttaagta ggaccaact ttttcacct 
      ttagcctga ctaattaac aagcattac tagtgacgt 
      ggcaaattt gttaattac tctaatggt acttaattt 
      agacaatat caatttcaa tattttctg tagcatctt 
      tctaatgta cctattttt gtaataaag gaaattata 
      caga
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