General information | |||
TC003689 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 12757775..12765166 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC003689 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00602 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
previtellogenic egg chamber not present - Number of animals showing the phenotype: 4
Comment: prooocyten absent |
- >TC003689 RA
MSVESQYYP DGDNEKRKR MSTGLQVNC PHSLRLAIQ SAYEYGYHF LVTQITHPN YARDLLHGK PPPAIGRTD RILQSLEWG RYIVAELTP TINVDSEIE HVQRKSKAL FLQELGFAV HLGVPVIKF SLTKRHNAQ LGRLINEKL VNGFTSSFW VTLPMVHPS QFSPICTED EKEDSWEWW NDFRTYCNY DKHVGLVLE LPEIAHIPS QSEVNRWIG EPVKALIIP TTYFILNNH GKPVLPRAH QDIIQRFLT IDVQYIIKS DSETDLSLY TKYLHFLGK KLYVGDPNL EFIQGCEDF LQNPLQPLT EHLETNIYE VFEKDQIKY TTYQNAIQK ALADVPQDV ALPVIMVVG AGRGPLVQA ALNVSYILH RKVKVYAVE KNPYAINTL IDRVNHDWN GQVTLINED MRVYEPPEK ADILVSELL GSFGDNELS PECLDGAQR FLKKSGISI PCSYTSYLA PLQSIKIFN EIRNNRPAD KTLRTCYET PYVIHLVNY YQIAPAQPL FKFEHPNWN DVINNERYK KLRFNCEQS CILTGFVGF FDTVLYKDV MLSIHPETH TREMVSWFP IVFPLMEPL KVEAGSVIE ISFWRVENA DKVWYEWCL EKPLKGCVM NPAGRSYFI KKH
- >TC003689 RA
atgagcgtg gaatctcaa tactaccca gacggagac aatgaaaaa cgcaagcga atgtcaacc ggtctccaa gtgaactgc ccccacagt cttaggcta gcaatccag tcagcttat gaatacggt taccatttt ctagtaact caaataacg caccccaac tacgctcgc gacctcctc cacgggaag cccccgccg gcaatcggt cgtacagac cgcatcttg caaagtctt gaatggggg cgctatatc gtcgctgaa ctcactccc accatcaac gtggacagc gaaattgaa cacgtgcaa aggaaaagc aaagccctg ttcttgcaa gagttaggt tttgcggtt catttgggg gttcccgtg ataaaattt tccctaact aagcgacac aacgcacag ttggggcgc ctcatcaat gagaaactc gtgaacggg tttacgtcc agtttttgg gttacgctc cccatggtg cacccgtca caatttagc cccatttgc accgaagat gagaaggag gattcttgg gagtggtgg aacgatttt cgcacttat tgtaattac gataagcac gtaggtcta gttttggag ttgcctgaa attgcacat attccttca caaagtgag gtcaatagg tggattggg gagcccgtc aaagcgcta attatcccc acgacttac tttatttta aataatcac ggaaagcct gtcttacca cgcgcccac caggacata attcagcgc tttctaacc atagacgtc caatacatc atcaaatcg gactctgag accgacctg tctctctac actaaatat ctacacttc ttggggaaa aagctatac gttggtgac cccaatctt gaatttatc caaggctgt gaagacttc ctgcaaaac cccctccag cccctcacc gagcacctt gaaaccaac atttacgaa gttttcgaa aaagaccaa atcaagtac actacatat cagaacgcc atccagaaa gccctcgca gacgtaccg caagacgtc gccctccct gtcatcatg gtcgtgggg gccggtcga ggccccctc gtccaagcc gccctcaac gtctcctac atcctccac cgtaaagtc aaagtctac gccgttgag aaaaatccc tacgctatc aacactcta attgatcga gtgaaccac gattggaac ggtcaagtc actctaatt aacgaagac atgcgagtg tatgagccc ccagagaag gccgatatt ttggtttcg gaactgttg gggtcgttt ggtgacaac gaactgtcg ccggagtgt ctcgatggg gctcaaaga tttctcaaa aagagtgga atctccatt ccgtgttcg tatacgtcg tatttggcg ccccttcag agtatcaag atttttaac gagattagg aacaatcgc ccggcggat aagacgcta agaacctgc tatgaaaca ccatatgtg attcatttg gttaattat taccaaata gctccagcc cagcctttg ttcaaattt gaacacccc aattggaat gatgttata aataatgaa aggtacaaa aaactgagg ttcaattgt gagcagagt tgcattctg acgggtttc gttggattt tttgacacc gttttgtac aaggatgtg atgttgagt atacaccca gaaacacac acccgtgag atggtcagt tggttccca atcgtgttc cctttaatg gaaccgtta aaagtcgag gctggtagc gtaattgaa atttcattt tggagagtc gaaaatgcc gacaaagtg tggtacgaa tggtgtttg gaaaaaccg ctcaaaggc tgcgtaatg aatccagcc ggacggtca tattttatt aaaaagcat tag
- >TC003689 RA
ggaactgtc aatttttat tttgacagc gttgtttga aaaatagtg gtaattcaa acgccccaa aatgagcgt ggaatctca atactaccc agacggaga caatgaaaa acgcaagcg aatgtcaac cggtctcca agtgaactg cccccacag tcttaggct agcaatcca gtcagctta tgaatacgg ttaccattt tctagtaac tcaaataac gcaccccaa ctacgctcg cgacctcct ccacgggaa gcccccgcc ggcaatcgg tcgtacaga ccgcatctt gcaaagtct tgaatgggg gcgctatat cgtcgctga actcactcc caccatcaa cgtggacag cgaaattga acacgtgca aaggaaaag caaagccct gttcttgca agagttagg ttttgcggt tcatttggg ggttcccgt gataaaatt ttccctaac taagcgaca caacgcaca gttggggcg cctcatcaa tgagaaact cgtgaacgg gtttacgtc cagtttttg ggttacgct ccccatggt gcacccgtc acaatttag ccccatttg caccgaaga tgagaagga ggattcttg ggagtggtg gaacgattt tcgcactta ttgtaatta cgataagca cgtaggtct agttttgga gttgcctga aattgcaca tattccttc acaaagtga ggtcaatag gtggattgg ggagcccgt caaagcgct aattatccc cacgactta ctttatttt aaataatca cggaaagcc tgtcttacc acgcgccca ccaggacat aattcagcg ctttctaac catagacgt ccaatacat catcaaatc ggactctga gaccgacct gtctctcta cactaaata tctacactt cttggggaa aaagctata cgttggtga ccccaatct tgaatttat ccaaggctg tgaagactt cctgcaaaa ccccctcca gcccctcac cgagcacct tgaaaccaa catttacga agttttcga aaaagacca aatcaagta cactacata tcagaacgc catccagaa agccctcgc agacgtacc gcaagacgt cgccctccc tgtcatcat ggtcgtggg ggccggtcg aggccccct cgtccaagc cgccctcaa cgtctccta catcctcca ccgtaaagt caaagtcta cgccgttga gaaaaatcc ctacgctat caacactct aattgatcg agtgaacca cgattggaa cggtcaagt cactctaat taacgaaga catgcgagt gtatgagcc cccagagaa ggccgatat tttggtttc ggaactgtt ggggtcgtt tggtgacaa cgaactgtc gccggagtg tctcgatgg ggctcaaag atttctcaa aaagagtgg aatctccat tccgtgttc gtatacgtc gtatttggc gccccttca gagtatcaa gatttttaa cgagattag gaacaatcg cccggcgga taagacgct aagaacctg ctatgaaac accatatgt gattcattt ggttaatta ttaccaaat agctccagc ccagccttt gttcaaatt tgaacaccc caattggaa tgatgttat aaataatga aaggtacaa aaaactgag gttcaattg tgagcagag ttgcattct gacgggttt cgttggatt ttttgacac cgttttgta caaggatgt gatgttgag tatacaccc agaaacaca cacccgtga gatggtcag ttggttccc aatcgtgtt ccctttaat ggaaccgtt aaaagtcga ggctggtag cgtaattga aatttcatt ttggagagt cgaaaatgc cgacaaagt gtggtacga atggtgttt ggaaaaacc gctcaaagg ctgcgtaat gaatccagc cggacggtc atattttat taaaaagca ttagtttca aattatttt tataataaa ttcataaca aattaaact gaaacaatt tgtttcccg tcctacaaa aaatttcct gcacatata tataccaat tttcttgtg agaattcat tcatgagtt gttctaatt cctgattta aatcacgag caagcgatt tgagtttct ctgtttggt caccaagac accaagcta tgctgatgt gaaacgaag gattttcat agaattttt ctaacttaa attcgccca aacaatgat caagacact gcactagtt ttagtaaag tgtcgtagc cagttttaa tctgtaaac ccaaaaaag ttcgggttt tttcctcat ttatgtaca cttccaggc catccaaca ttataataa atctgtaaa aaatta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
