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Gene information
General information
TC003655 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid17: 101753..105357
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003655
    iBeetle screen iB_00598
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    vitellarium shortened - Number of animals showing the phenotype: 4
    vitellogenic egg chamber not present - Number of animals showing the phenotype: 4
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003655 RA
      MGKLNVTIM RYLTGEDFR VLTAIEMGM KNHELVPGP 
      MAASIANLQ HGGVHKLLR DLCKHGLLS YERGRKYDG 
      YRLTNMGYD YLALHSLTK RDVIGSFGN QIGVGKESN 
      IYIVANPEG EEVCLKLHR LGRVCFRNV TNKRDYHKH 
      RKSASWLYL SRISATKEF AYMKALYDR KFPVPKPID 
      FNRHCVIME LVKGRPLCH VMELNDVEA LYDELMNLI 
      VKFADSGVI HGDFNEFNI MIKDDEKPV IIDFPQMMS 
      TAHENAEMY FNRDVNCIK TFFKKRFGY ESELYPKFS 
      DIERTDALD AEVLCSGFT KDMAKDINR ELGIDEETG 
      SEDEEESQD TEEKCTVDS IFDQLKLQD RETQSDSES 
      IDSFDRFES GSVRSCATT IHPDEIRSR VKKQIVSRE 
      KKTQRKKCV AKGEASATT RVRRENNDT IKQSKGLWG 
      WE
    CDS sequence
    • >TC003655 RA
      atggggaaa ttgaacgta acaataatg cgttacctc 
      accggtgag gatttccgg gttctaaca gcgattgag 
      atgggcatg aaaaaccac gaattggtt ccgggcccc 
      atggctgcg tccattgcg aatttgcag cacggcggt 
      gttcacaaa ttgctgcgt gatttgtgc aagcacgga 
      ttattgagt tacgagcgg gggcgcaaa tacgatggt 
      taccgcttg accaacatg gggtatgac tatctggcc 
      ctccattcg ttaacaaaa cgcgacgtt attggttct 
      tttggaaat cagattggg gtggggaag gagagtaat 
      atttatatt gttgcaaat cctgaagga gaggaagtt 
      tgtttgaag ttgcataga ttgggacga gtgtgtttc 
      cggaatgtt acgaataaa cgagattat cacaagcac 
      agaaaatcg gcgtcttgg ttgtactta tcaagaatt 
      tcagccact aaagaattt gcttacatg aaggcgctg 
      tatgatagg aagtttcca gtccctaaa cccattgat 
      tttaataga cattgtgtc atcatggag ttggtcaaa 
      gggcggccc ttgtgtcac gtaatggaa ttaaatgac 
      gtggaggcg ctttacgat gaattaatg aatcttatt 
      gttaagttt gccgatagt ggagtgatt catggtgat 
      ttcaatgag tttaacata atgataaaa gacgatgag 
      aaacctgta ataattgat ttccctcaa atgatgtca 
      acagctcat gaaaacgct gaaatgtat tttaatagg 
      gacgttaac tgtattaaa actttcttt aaaaaacga 
      tttggttat gagagcgaa ttataccca aaattcagc 
      gatatcgaa agaacggat gctctagac gctgaggtt 
      ttgtgttca gggtttacg aaagacatg gctaaagat 
      attaaccga gaattgggt atcgatgaa gaaactggg 
      agcgaagac gaagaggaa tcacaagat actgaagaa 
      aaatgcaca gtcgattca atttttgat cagttgaaa 
      ctccaagat cgcgaaacc caatcagat agtgaatca 
      attgattcg tttgataga tttgaaagt ggaagtgtg 
      aggtcttgc gcgaccacg atccaccca gatgaaatc 
      agaagtcgc gtcaaaaaa caaatcgta agtagggaa 
      aagaagact caaaggaag aaatgtgta gctaagggc 
      gaagctagc gccactaca agggtccga agggagaat 
      aacgatacc attaaacag agcaaagga ctgtgggga 
      tgggaatga
    mRNA sequence
    • >TC003655 RA
      tagaaacaa tctcaaacc ttgcaaatg tgttgatgc 
      aattgcgtg aaaaatagt taatttaag tgttagaaa 
      tggggaaat tgaacgtaa caataatgc gttacctca 
      ccggtgagg atttccggg ttctaacag cgattgaga 
      tgggcatga aaaaccacg aattggttc cgggcccca 
      tggctgcgt ccattgcga atttgcagc acggcggtg 
      ttcacaaat tgctgcgtg atttgtgca agcacggat 
      tattgagtt acgagcggg ggcgcaaat acgatggtt 
      accgcttga ccaacatgg ggtatgact atctggccc 
      tccattcgt taacaaaac gcgacgtta ttggttctt 
      ttggaaatc agattgggg tggggaagg agagtaata 
      tttatattg ttgcaaatc ctgaaggag aggaagttt 
      gtttgaagt tgcatagat tgggacgag tgtgtttcc 
      ggaatgtta cgaataaac gagattatc acaagcaca 
      gaaaatcgg cgtcttggt tgtacttat caagaattt 
      cagccacta aagaatttg cttacatga aggcgctgt 
      atgatagga agtttccag tccctaaac ccattgatt 
      ttaatagac attgtgtca tcatggagt tggtcaaag 
      ggcggccct tgtgtcacg taatggaat taaatgacg 
      tggaggcgc tttacgatg aattaatga atcttattg 
      ttaagtttg ccgatagtg gagtgattc atggtgatt 
      tcaatgagt ttaacataa tgataaaag acgatgaga 
      aacctgtaa taattgatt tccctcaaa tgatgtcaa 
      cagctcatg aaaacgctg aaatgtatt ttaataggg 
      acgttaact gtattaaaa ctttcttta aaaaacgat 
      ttggttatg agagcgaat tatacccaa aattcagcg 
      atatcgaaa gaacggatg ctctagacg ctgaggttt 
      tgtgttcag ggtttacga aagacatgg ctaaagata 
      ttaaccgag aattgggta tcgatgaag aaactggga 
      gcgaagacg aagaggaat cacaagata ctgaagaaa 
      aatgcacag tcgattcaa tttttgatc agttgaaac 
      tccaagatc gcgaaaccc aatcagata gtgaatcaa 
      ttgattcgt ttgatagat ttgaaagtg gaagtgtga 
      ggtcttgcg cgaccacga tccacccag atgaaatca 
      gaagtcgcg tcaaaaaac aaatcgtaa gtagggaaa 
      agaagactc aaaggaaga aatgtgtag ctaagggcg 
      aagctagcg ccactacaa gggtccgaa gggagaata 
      acgatacca ttaaacaga gcaaaggac tgtggggat 
      gggaatgaa attcaagag ttataattt aattttaat 
      ttaataaaa gtttgtcta ttag