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Gene information
General information
TC003648 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10573912..10575705
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003648
    iBeetle screen iB_00597

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature mostly orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature sometimes thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shape irregular -

    legs segment shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs femur fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs tibiotarsus fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs coxa not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs trochanter not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003648 RA
      MSILNTSVT KQPLKLIEV PLNKFSEEV IPHHQNIFE 
      QHKTLVLKL ISQNNGQRV RQEVKNKKR IVKQLKDLM 
      YELDTLRAQ VEDSDLDKF DTKTLSFRK IIITLISGY 
      TDLEKRSLA LDNQNQDDE DEKRLLRER CPFEGASQI 
      QLQDNLEDI KLRQRQELL NEVETVQRD TEDLNQIFT 
      HLNQMVEGQ REQVEHVEE TIEESKENV QSGFKQLVK 
      AHKLKTAVY PVTGAFLGS MIGGPVGLV AGFKIGGLT 
      ALGCAIAGY TGGKFFKKN HEVEEIEEK KEESVQMDD 
      KHEKKDI
    CDS sequence
    • >TC003648 RA
      atgtcgatt ttaaacaca agtgttacg aaacaaccc 
      ctgaaactg atcgaagtg cctttaaat aaattcagt 
      gaagaagtg atccctcac caccagaat attttcgag 
      cagcacaaa acgctagtc ctcaagttg atctcgcaa 
      aacaatggc caacgcgtc cgccaagag gtgaaaaac 
      aaaaaacga atcgtaaaa caattgaaa gacttgatg 
      tacgaactg gatacttta agggcacaa gtcgaagac 
      tccgattta gataaattc gacaccaaa acgctatca 
      tttaggaaa attatcatc accctcatt tcgggttac 
      acagattta gagaaacga agtttagca ttagacaac 
      caaaaccag gacgatgaa gacgagaag agactcctg 
      cgtgagcgg tgccccttt gagggggcg tcgcaaatc 
      caactccaa gacaaccta gaggacatc aaactgaga 
      caacgacaa gaactccta aacgaggtg gaaacggtt 
      caaagagac actgaagac ttgaaccaa attttcacc 
      catttgaac caaatggtt gaagggcaa agggagcag 
      gtcgagcat gttgaggaa acgattgaa gagagtaaa 
      gaaaacgtg cagtcgggg tttaaacaa ctcgtcaaa 
      gcccacaaa ttgaaaact gccgtttat ccagtcact 
      ggtgccttc ctggggtca atgataggg ggccctgtt 
      ggtttagtg gcagggttt aaaatcggg ggtttgacg 
      gctttaggg tgtgcgata gccgggtat acagggggg 
      aaatttttc aaaaaaaat cacgaagtg gaagaaatt 
      gaggagaaa aaggaagag agcgtgcaa atggatgac 
      aaacacgag aaaaaggat atatga
    mRNA sequence
    • >TC003648 RA
      ttgccggcc caaatgtca acaagcaag ttttttggt 
      tagaaacgt caaaattct ttgtgctac aactccatt 
      atttacaaa taaacacag taatgtggt aacaatgtc 
      gattttaaa cacaagtgt tacgaaaca acccctgaa 
      actgatcga agtgccttt aaataaatt cagtgaaga 
      agtgatccc tcaccacca gaatatttt cgagcagca 
      caaaacgct agtcctcaa gttgatctc gcaaaacaa 
      tggccaacg cgtccgcca agaggtgaa aaacaaaaa 
      acgaatcgt aaaacaatt gaaagactt gatgtacga 
      actggatac tttaagggc acaagtcga agactccga 
      tttagataa attcgacac caaaacgct atcatttag 
      gaaaattat catcaccct catttcggg ttacacaga 
      tttagagaa acgaagttt agcattaga caaccaaaa 
      ccaggacga tgaagacga gaagagact cctgcgtga 
      gcggtgccc ctttgaggg ggcgtcgca aatccaact 
      ccaagacaa cctagagga catcaaact gagacaacg 
      acaagaact cctaaacga ggtggaaac ggttcaaag 
      agacactga agacttgaa ccaaatttt cacccattt 
      gaaccaaat ggttgaagg gcaaaggga gcaggtcga 
      gcatgttga ggaaacgat tgaagagag taaagaaaa 
      cgtgcagtc ggggtttaa acaactcgt caaagccca 
      caaattgaa aactgccgt ttatccagt cactggtgc 
      cttcctggg gtcaatgat agggggccc tgttggttt 
      agtggcagg gtttaaaat cgggggttt gacggcttt 
      agggtgtgc gatagccgg gtatacagg ggggaaatt 
      tttcaaaaa aaatcacga agtggaaga aattgagga 
      gaaaaagga agagagcgt gcaaatgga tgacaaaca 
      cgagaaaaa ggatatatg acagtgtgt tctttgtta 
      atttatttt aaataaaag acgtctgac aaagatagg 
      aaactaact atggtaaaa aatcgaatc atgtttatc 
      atcttttct tagaaataa atgtcaaat atcgtgaaa 
      tttttgtgt gataatttt ttaggtatt acctatttg 
      taagtgtaa atagttcaa taaattgaa ttctagaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY