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Gene information
General information
TC003642 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10268953..10274969
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003642
    iBeetle screen iB_00596

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003642 RA
      MLGRQKLKY EAVDSDDEP PSDFEEKKR GFFNNNCFW 
      SSLFTSVLI GTYYIPSIC LTFYQRWLF QTFHFPLVT 
      VLVHMIVKF LLAALIRAV LERRQGKQR VMLEWREYL 
      VAVAPMGVF SGLDIGFSN WGLELIKVS LYTMTKSTT 
      VVFILGFSM LFKLEKKSW SLALIVGMI TTGLILFTY 
      KATQFDTLG FLLLLLASM SSGVRWTCV QLLLQKSKI 
      GMRNPIDMI YHMQPWMII SVLPFAIWM EGPSVVKNC 
      QLIRTTDTS IIITMIFKI LLGAFIAFF MEVCEVLVV 
      GYTSSLTLS IAGVVKEVF ILVLAVEWN GDQLSPINV 
      VGLLICLSG ITCHVFHKL RNTPKVARV YEVHDERRE 
      LGEHLIVSE DGHVSSDND EKNDTQVLF DILRNRDR
    CDS sequence
    • >TC003642 RA
      atgttgggc cgtcaaaaa ttaaaatac gaagctgtg 
      gacagcgac gatgagccc ccgagtgat ttcgaggaa 
      aagaaacgt ggttttttc aacaataat tgcttttgg 
      tccagttta tttacaagt gttttgatt ggaacttac 
      tacattcct tccatatgt ttgacgttc tatcagcga 
      tggcttttc cagacattc cattttccg ttagtcacg 
      gttttggtt catatgatt gtcaaattt ctactagct 
      gcgctaatc agggctgtg ttggagcga agacagggc 
      aaacaaagg gttatgttg gagtggagg gagtatctg 
      gtcgcagtg gcccccatg ggggtcttc agtggccta 
      gacataggc ttctctaat tggggctta gaactcatt 
      aaagtctca ttatataca atgaccaag tccacgaca 
      gttgtgttt attttggga ttttcgatg ctatttaag 
      cttgaaaaa aagtcatgg agtcttgcc cttatcgtt 
      gggatgatc acaacggga ttaatcctc tttacttac 
      aaagcgacc caatttgac actttgggc tttttgttg 
      cttttattg gcctcgatg tctagtgga gtgagatgg 
      acttgcgtc caactcctc ctccagaag tccaaaatc 
      gggatgaga aaccccatc gatatgatc taccacatg 
      cagccgtgg atgatcatc tcagtccta ccatttgcc 
      atatggatg gaaggcccc tcagttgta aaaaattgc 
      caactgata cgcaccaca gacacctca ataataatc 
      acaatgatt ttcaaaatt ttactcggg gcctttatc 
      gcatttttc atggaagtg tgcgaagtc ctagtggtg 
      ggctacacc tcaagtctc acgctctcc atcgctgga 
      gttgttaaa gaagtgttt atcctagtc ttagccgta 
      gaatggaac ggggaccaa ctatcgccc atcaacgtt 
      gttggcttg cttatttgt ctctctgga atcacgtgc 
      catgttttc cacaagttg aggaacact ccgaaggtg 
      gcacgtgtg tatgaggtg cacgacgag cgacgcgag 
      ctgggtgag catttaata gtcagtgaa gatggacac 
      gtgtcgagt gataacgac gagaagaac gacacgcaa 
      gtactgttc gatattttg cgcaatcgg gacaggtag
    mRNA sequence
    • >TC003642 RA
      tcaaacgtc actcggtcc tgtcaactt gtcacaaaa 
      cagaaaaaa agtgaaata aaccaaaaa aagtgcatt 
      tattccacg tctacggaa aatgtgacc ttatcatgg 
      attttccgc gccacgaaa ccttatctt cgcccacaa 
      accacagta aaatcccta tcaacatgt aaacaagat 
      gttgggccg tcaaaaatt aaaatacga agctgtgga 
      cagcgacga tgagccccc gagtgattt cgaggaaaa 
      gaaacgtgg ttttttcaa caataattg cttttggtc 
      cagtttatt tacaagtgt tttgattgg aacttacta 
      cattccttc catatgttt gacgttcta tcagcgatg 
      gcttttcca gacattcca ttttccgtt agtcacggt 
      tttggttca tatgattgt caaatttct actagctgc 
      gctaatcag ggctgtgtt ggagcgaag acagggcaa 
      acaaagggt tatgttgga gtggaggga gtatctggt 
      cgcagtggc ccccatggg ggtcttcag tggcctaga 
      cataggctt ctctaattg gggcttaga actcattaa 
      agtctcatt atatacaat gaccaagtc cacgacagt 
      tgtgtttat tttgggatt ttcgatgct atttaagct 
      tgaaaaaaa gtcatggag tcttgccct tatcgttgg 
      gatgatcac aacgggatt aatcctctt tacttacaa 
      agcgaccca atttgacac tttgggctt tttgttgct 
      tttattggc ctcgatgtc tagtggagt gagatggac 
      ttgcgtcca actcctcct ccagaagtc caaaatcgg 
      gatgagaaa ccccatcga tatgatcta ccacatgca 
      gccgtggat gatcatctc agtcctacc atttgccat 
      atggatgga aggcccctc agttgtaaa aaattgcca 
      actgatacg caccacaga cacctcaat aataatcac 
      aatgatttt caaaatttt actcggggc ctttatcgc 
      atttttcat ggaagtgtg cgaagtcct agtggtggg 
      ctacacctc aagtctcac gctctccat cgctggagt 
      tgttaaaga agtgtttat cctagtctt agccgtaga 
      atggaacgg ggaccaact atcgcccat caacgttgt 
      tggcttgct tatttgtct ctctggaat cacgtgcca 
      tgttttcca caagttgag gaacactcc gaaggtggc 
      acgtgtgta tgaggtgca cgacgagcg acgcgagct 
      gggtgagca tttaatagt cagtgaaga tggacacgt 
      gtcgagtga taacgacga gaagaacga cacgcaagt 
      actgttcga tattttgcg caatcggga caggtagtc 
      gcgagtttt cttcatgac tggatagta cttaaggaa 
      atgtgggtg tgagtcacc cagattttt taatttaat 
      acttttata acaacataa gattatgtt ttgtgatat 
      tgtgtaatt taaggctaa gtgtatgtg tgacaatgt 
      gattgtttt ttattgagt gaggggtaa ggttgcgat 
      gtcgctaaa gttacgtct ttttttaat tattcttct 
      actggacga ttttaaaag ccggtaata aaattttcg 
      aatctccct taaaaaaaa tttcttgtc cattttggt 
      gaaactttg gacaatttt aaggaagtt ttgagctcc 
      acctgtgta aaatgttcc gaggagttg ataaaatta 
      gacatttta cgagttatg aatggattt aactttaac 
      acaagtgca ttagctcat actccaaac atgcactat 
      tatccgaac acttcataa taattattt tttcaaaaa 
      ttgaatata ctttgaata gacttatac tgtatattt 
      tgttataca tgaagtact agcgagttt ataaaataa 
      atctttaaa tagtgaaat atttaaggt agtacttta 
      ataagcatt tacttttta ttactacta attactagt 
      aattttcgt actcaagtc ttaatagct gttagctat 
      tcggccgtt attatatat atttttttg aaaatggct 
      taattttta aatatcaac ttaatgtca aacgctcaa 
      ggcaatcta atagttgct gccatgtta cactcattg 
      gtcacaagt tgtcctcat ggtcaccac actgcattc 
      ccattggtc acaagcctg ataatcaaa aatctgtat 
      ggtaaaaaa ttttcatta aagtacaga ttacagaca 
      ttttatcaa ccaaataaa gcatgggag tagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY