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Gene information
General information
TC003620 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 4711706..4714503
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003620
    iBeetle screen iB_00592
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    egg chamber sometimes shape rounded - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003620 RA
      MWKVALFST LVTAFFFIH QARTCNEAV 
      CGSIVSKCL LTQSCKCDF SFENCTCCK 
      DCFNCLSYL YAECCSCVD MCPKPNDTE 
      GALSKKSNV EDFSEPVPG LFNVLTEYP 
      DSERRWNST TFPIDIDTS QYGPKKEIK 
      IHMQSAEQE VAPIKTNVI TLNCTVAFM 
      SKCMSLTKC RSSCQTMGA SSYRWFHDG 
      CCECVGQHC INYGINESR CRECPLKDE 
      NELSEEEFD VDDYDYGQN SEDFE
    CDS sequence
    • >TC003620 RA
      atgtggaaa gttgcgctt ttttcgacg ctggtgacg 
      gcgttcttc ttcatccac caggcccgg acgtgcaac 
      gaggcggtg tgtggcagc atagtcagc aagtgcctt 
      ctgacgcag tcctgcaag tgcgacttt tccttcgaa 
      aactgcacc tgctgcaag gactgcttc aactgtctc 
      agctacctc tatgccgag tgctgctct tgcgttgac 
      atgtgtccg aaaccaaac gacaccgaa ggcgccctc 
      agcaaaaaa tccaacgtt gaggacttc agtgagccc 
      gtccctggg ctttttaac gttttgacc gaatatccg 
      gatagtgag cgacgatgg aacagcact accttcccc 
      attgatatc gatacgagt caatacgga ccgaagaaa 
      gaaatcaaa attcatatg caaagtgcc gagcaagag 
      gtggccccc atcaaaacc aacgttatc acgcttaac 
      tgcacggtt gccttcatg tcaaaatgt atgagcttg 
      accaagtgc aggtccagc tgccagact atgggggct 
      tccagctat cgctggttt cacgatggg tgctgcgag 
      tgcgtgggg caacactgc atcaattat gggataaat 
      gagagcagg tgtcgcgaa tgcccgttg aaagacgag 
      aatgagctt tcagaggaa gaattcgat gtcgacgat 
      tatgactat ggccagaat tcggaagat ttcgaataa
    mRNA sequence
    • >TC003620 RA
      cttttacct ttaaacttg ccgcgacga gaaaacggt 
      ggggctcag tagcaaaac aagtttgag aagagaagc 
      atgatgttg agagtttat ccagaaatt tgtctctag 
      tttagtgcc ttccaagaa tatttccag tcccaaaac 
      ggagtcaac aactgacta aacatgtgg aaagttgcg 
      cttttttcg acgctggtg acggcgttc ttcttcatc 
      caccaggcc cggacgtgc aacgaggcg gtgtgtggc 
      agcatagtc agcaagtgc cttctgacg cagtcctgc 
      aagtgcgac ttttccttc gaaaactgc acctgctgc 
      aaggactgc ttcaactgt ctcagctac ctctatgcc 
      gagtgctgc tcttgcgtt gacatgtgt ccgaaacca 
      aacgacacc gaaggcgcc ctcagcaaa aaatccaac 
      gttgaggac ttcagtgag cccgtccct gggcttttt 
      aacgttttg accgaatat ccggatagt gagcgacga 
      tggaacagc actaccttc cccattgat atcgatacg 
      agtcaatac ggaccgaag aaagaaatc aaaattcat 
      atgcaaagt gccgagcaa gaggtggcc cccatcaaa 
      accaacgtt atcacgctt aactgcacg gttgccttc 
      atgtcaaaa tgtatgagc ttgaccaag tgcaggtcc 
      agctgccag actatgggg gcttccagc tatcgctgg 
      tttcacgat gggtgctgc gagtgcgtg gggcaacac 
      tgcatcaat tatgggata aatgagagc aggtgtcgc 
      gaatgcccg ttgaaagac gagaatgag ctttcagag 
      gaagaattc gatgtcgac gattatgac tatggccag 
      aattcggaa gatttcgaa taataaaaa tacatacag 
      actgaatca