Status
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Gene information
General information
TC003619 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9266573..9271191
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003619
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00591

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003619 RA
      MYSQLNKYR VPLKAVCKF SLLRKKPEI LNIADNGLT 
      QKRVKTHVR LDQINSKLI KGVSREYSA VCSLIHRSG 
      LQNLSHLPY YLDKREFHS SVKNLQQSS GGKPGGDKN 
      KPNDDDDKD KISSLLAKA FLWMLTAYM VIAIISLMF 
      PSSSQPEVV RYVSWNEFL YQMLAKGEV EEIIVRPDI 
      EIVTIILHE GAIIKGKKV DNRTYHMNV VDINKFEDK 
      LREAERRLG IQDGIPITF ERGTDTAGK LLISLLIVA 
      ILISLLARS KSIRPPISM DTFTQLGRA KFTLVDPLS 
      GQGKGVHFS DVAGLREAK QEVMEFVDY LKRPEIYKS 
      LGAKVPKGA LLLGPPGCG KTLLAKAVA TEANVPFLS 
      MNGSEFIEM IGGLGAARV RDLFKEARK RAPCIIYID 
      EIDAVGRKR SGQLGQGGA SGESEQTLN QLLVEMDGM 
      ASKESVVML ASTNRADIL DRALLRPGR FDRHILIDL 
      PDLEERKQI FELHLKSIA LENEPEFYS KRLAHLTPG 
      FSGADIANV CNEAALHAA REKQKHVTR DNLEYAVER 
      LVGGTEKRN HAMSPEEKR IVAYHESGH ALVGWMLEH 
      TDALLKVTI VPRTNLALG FAQYIPRDQ KLYTKEELF 
      ERMCMTLGG RVAESLIFN RITTGAQND LEKVTKMAY 
      AQIKEFGMS ESVGLLSFP QEETQEKGR RPYSKKLAN 
      LIDIEARRL VSRAYKRTE EVLLKNKEK LELLAETLL 
      KKETLNYDD VEKLLGPPP FGAKHLIEP AEFEESVRK 
      DAGDTRPTE TKSETNASA PKI
    CDS sequence
    • >TC003619 RA
      atgtatagc cagttaaat aagtatcgt gtgccactg 
      aaggctgta tgcaaattt tccttgtta aggaaaaaa 
      ccagaaatt ctaaacatt gctgataat gggttgact 
      caaaaacgg gtgaaaacc cacgttaga ttagatcaa 
      atcaactcg aaattaatt aagggtgta tctagggaa 
      tactctgcc gtgtgttca cttatccac cggtcaggg 
      ttacaaaat ttgagccac ttgccgtac tatttggat 
      aaaagagag ttccactct tcggtgaaa aatttgcag 
      cagtcttcc gggggcaaa cctggcggt gataagaat 
      aaaccaaac gatgatgac gataaagac aagatttcg 
      tcattgttg gctaaggcc tttctgtgg atgcttaca 
      gcctacatg gtcatagca ataatttcg ttgatgttt 
      cctagtagt agccaacca gaggttgta cgatatgtc 
      tcctggaac gaatttttg taccaaatg ttggccaaa 
      ggcgaagtg gaagaaatt atcgtcagg cctgatatc 
      gaaatcgtt acgattatc ctacacgaa ggtgccata 
      attaaagga aaaaaagtt gataacagg acgtatcac 
      atgaatgtt gtcgatatc aacaaattc gaagacaaa 
      ctcagagag gctgaacgt cgtttaggc atccaagac 
      ggaatcccg atcacattt gaacgcgga acggacaca 
      gccggaaaa ctactcatc tctctcctg atcgttgca 
      atccttatt tcccttctc gctcgcagc aaaagcata 
      agaccgcca atcagcatg gacacattt acgcaatta 
      gggcgcgct aagttcacc cttgttgac cctctgtca 
      ggtcagggc aaaggggtg cacttttca gacgtggca 
      ggtttaagg gaagcgaaa caggaagta atggagttt 
      gttgattat ttgaaacgg cctgagatt tacaagagt 
      ttgggggcg aaggtgcct aagggggca ctgctttta 
      ggcccccct ggttgtggt aagacttta ctggccaaa 
      gctgtggcc actgaagcc aatgtgcct tttttgtct 
      atgaatggg tcagagttt atcgagatg attggcggc 
      ttgggggcg gcaagggta agggacttg ttcaaagag 
      gcgcgcaaa cgcgccccc tgtataatc tacatcgac 
      gaaatcgac gcagtaggc cgtaaacga agcggccaa 
      ttgggccaa ggtggcgcc agtggcgaa agtgagcag 
      actttgaac cagcttttg gtggaaatg gacggaatg 
      gcaagcaag gaaagtgtc gttatgctt gcctccacc 
      aacagggcc gatattttg gacagggcc ctactacgt 
      cccggacgc ttcgacaga cacatttta atcgacttg 
      cctgatcta gaagaacga aaacaaatt tttgagcta 
      catttgaag agtatagca ttggaaaac gagcctgaa 
      ttctattcg aaaaggttg gcgcacttg acacccgga 
      ttcagtggc gctgacatc gccaatgtt tgcaacgag 
      gcggctttg cacgcggca agagagaaa caaaagcac 
      gtgacgcgg gacaattta gaatatgcc gttgaacgt 
      ctcgttgga ggtaccgaa aaacgcaac catgccatg 
      tcccctgaa gaaaaacgc atcgttgcc tatcacgaa 
      tccggtcat gccctagtt ggctggatg ttggagcac 
      actgatgcc ctcctgaag gtcactata gtccctagg 
      accaaccta gcgctgggt tttgcccag tacatccct 
      cgggaccag aaattgtac accaaggag gaattattc 
      gaaaggatg tgcatgact ctaggtggc cgagtggct 
      gaatcattg atttttaac cgaattaca acgggggcc 
      caaaacgat ttagaaaaa gtaaccaaa atggcctac 
      gcccaaatc aaggagttt gggatgagt gaaagtgtg 
      ggtttgttg tcgttcccc caggaagag acgcaggag 
      aagggccga aggccctac agtaaaaaa ttagcgaat 
      cttatagac attgaggcg aggagattg gtcagtagg 
      gcgtacaaa cgcacagag gaggttttg ttgaagaat 
      aaagaaaaa cttgaattg cttgcggag acgttgttg 
      aagaaggag actttaaac tatgatgat gtcgagaag 
      ctgttgggg cctccgccc tttggggcc aagcacttg 
      atcgaaccg gctgagttt gaggagtct gtgagaaag 
      gatgctggg gatactagg ccaactgag accaagtct 
      gagactaat gcgtctgcg cccaaaatt tga
    mRNA sequence
    • >TC003619 RA
      attatttta aaagttggt agaggacag cacgtctca 
      gagtgtcat cttttgacg tttatgtaa cctcaaatt 
      ttcgccaaa atgaaaaaa tacaaaaat gtatagcca 
      gttaaataa gtatcgtgt gccactgaa ggctgtatg 
      caaattttc cttgttaag gaaaaaacc agaaattct 
      aaacattgc tgataatgg gttgactca aaaacgggt 
      gaaaaccca cgttagatt agatcaaat caactcgaa 
      attaattaa gggtgtatc tagggaata ctctgccgt 
      gtgttcact tatccaccg gtcagggtt acaaaattt 
      gagccactt gccgtacta tttggataa aagagagtt 
      ccactcttc ggtgaaaaa tttgcagca gtcttccgg 
      gggcaaacc tggcggtga taagaataa accaaacga 
      tgatgacga taaagacaa gatttcgtc attgttggc 
      taaggcctt tctgtggat gcttacagc ctacatggt 
      catagcaat aatttcgtt gatgtttcc tagtagtag 
      ccaaccaga ggttgtacg atatgtctc ctggaacga 
      atttttgta ccaaatgtt ggccaaagg cgaagtgga 
      agaaattat cgtcaggcc tgatatcga aatcgttac 
      gattatcct acacgaagg tgccataat taaaggaaa 
      aaaagttga taacaggac gtatcacat gaatgttgt 
      cgatatcaa caaattcga agacaaact cagagaggc 
      tgaacgtcg tttaggcat ccaagacgg aatcccgat 
      cacatttga acgcggaac ggacacagc cggaaaact 
      actcatctc tctcctgat cgttgcaat ccttatttc 
      ccttctcgc tcgcagcaa aagcataag accgccaat 
      cagcatgga cacatttac gcaattagg gcgcgctaa 
      gttcaccct tgttgaccc tctgtcagg tcagggcaa 
      aggggtgca cttttcaga cgtggcagg tttaaggga 
      agcgaaaca ggaagtaat ggagtttgt tgattattt 
      gaaacggcc tgagattta caagagttt gggggcgaa 
      ggtgcctaa gggggcact gcttttagg cccccctgg 
      ttgtggtaa gactttact ggccaaagc tgtggccac 
      tgaagccaa tgtgccttt tttgtctat gaatgggtc 
      agagtttat cgagatgat tggcggctt gggggcggc 
      aagggtaag ggacttgtt caaagaggc gcgcaaacg 
      cgccccctg tataatcta catcgacga aatcgacgc 
      agtaggccg taaacgaag cggccaatt gggccaagg 
      tggcgccag tggcgaaag tgagcagac tttgaacca 
      gcttttggt ggaaatgga cggaatggc aagcaagga 
      aagtgtcgt tatgcttgc ctccaccaa cagggccga 
      tattttgga cagggccct actacgtcc cggacgctt 
      cgacagaca cattttaat cgacttgcc tgatctaga 
      agaacgaaa acaaatttt tgagctaca tttgaagag 
      tatagcatt ggaaaacga gcctgaatt ctattcgaa 
      aaggttggc gcacttgac acccggatt cagtggcgc 
      tgacatcgc caatgtttg caacgaggc ggctttgca 
      cgcggcaag agagaaaca aaagcacgt gacgcggga 
      caatttaga atatgccgt tgaacgtct cgttggagg 
      taccgaaaa acgcaacca tgccatgtc ccctgaaga 
      aaaacgcat cgttgccta tcacgaatc cggtcatgc 
      cctagttgg ctggatgtt ggagcacac tgatgccct 
      cctgaaggt cactatagt ccctaggac caacctagc 
      gctgggttt tgcccagta catccctcg ggaccagaa 
      attgtacac caaggagga attattcga aaggatgtg 
      catgactct aggtggccg agtggctga atcattgat 
      ttttaaccg aattacaac gggggccca aaacgattt 
      agaaaaagt aaccaaaat ggcctacgc ccaaatcaa 
      ggagtttgg gatgagtga aagtgtggg tttgttgtc 
      gttccccca ggaagagac gcaggagaa gggccgaag 
      gccctacag taaaaaatt agcgaatct tatagacat 
      tgaggcgag gagattggt cagtagggc gtacaaacg 
      cacagagga ggttttgtt gaagaataa agaaaaact 
      tgaattgct tgcggagac gttgttgaa gaaggagac 
      tttaaacta tgatgatgt cgagaagct gttggggcc 
      tccgccctt tggggccaa gcacttgat cgaaccggc 
      tgagtttga ggagtctgt gagaaagga tgctgggga 
      tactaggcc aactgagac caagtctga gactaatgc 
      gtctgcgcc caaaatttg agtgtattg tagataatg 
      agactttga acaaaatga ataaatgtt tttaaaaag 
      t
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