Status
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Gene information
General information
TC003602 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8692515..8695179
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003602
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00588

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    remarks: preps lost,
    all eggs dissolved during bleeching phase

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003602 RA
      MAWRKFVSL DYKVHLLVS LVVRLALVA YGVYHDQVS 
      SVPYTDIDY KVFTDAARH VLDHKSPFE RHTYRYSPL 
      VAYLMIPNI TLHSSFGKI LFCIIDLID ATLIRIIVK 
      TTLHEYFKY VGEPQNCIT GRSRKKTKT KKNDQTDTT 
      ANLSLVVWL YNPLTLAIA TRGNCDSIA VFFVLATLY 
      LLQCRKQYF AAGLIHGLS IHFRLYPIV YSLTFYMYL 
      SKFSFYSLE DRRKSHLRE ITGASDGRV ATRKPERKT 
      IFKRKYLLY LVPNFDQIR LVQGCILSL FSLIGIFYF 
      LYGYKFVYE TYLYHFTRK DTRHNFSLY FYLQYLTAW 
      IKNIGIWQK VLVVLPQLV LLLVFSFRY GLNRFSLNF 
      SVLAQTIVM VTYNSVLTS QYFVWIMAV LPLCLWQIR 
      MSVNNAIFL VVVWFAAQG AWLLPAYFL EFHGQNTFL 
      FIWIQSVSF FCANIAILG RLIMFFAPV KKEV
    CDS sequence
    • >TC003602 RA
      atggcgtgg cgtaaattc gttagtcta gattacaaa 
      gtccatttg cttgtttcc cttgttgtt aggctggct 
      ctagtggcc tatggggtc taccatgat caggtctcc 
      tcagtccct tacacggac atcgactat aaagtcttc 
      acagacgcg gcacgacac gtccttgac cacaaatcg 
      ccctttgaa agacacact tatcgctat tcgcccctt 
      gtggcctat ctcatgatt cccaacatc accctccac 
      tccagcttc ggcaaaatc ctgttctgc attatcgac 
      ttaatcgac gccaccttg atccgaata atagtcaaa 
      acgacgctc cacgaatac ttcaagtac gtgggggaa 
      ccccaaaac tgcatcacg gggcgcagc cgcaagaaa 
      accaaaacg aagaaaaac gaccaaact gacaccact 
      gcgaattta agtctggta gtctggctt tacaacccc 
      ctaactcta gccattgcc actcgagga aattgtgac 
      tcgatcgcg gtttttttc gtgttggca acgctgtat 
      ttgttgcaa tgtcgcaaa caatatttt gcggccgga 
      ttaatacac ggtctttcg atacatttc cgcctgtac 
      ccgatcgtg tacagcctc acgttttac atgtatttg 
      agcaagttt tcattttac tcgctggaa gataggcgc 
      aaaagccac ttgagggaa atcaccggt gcgagcgac 
      ggcagggtt gccacccgc aagccggag agaaaaacg 
      attttcaag aggaaatat ctgctttat ttggtcccg 
      aatttcgat cagataagg ctcgtccag ggctgtatc 
      ttgtcgctt ttctccctg attggcata ttttatttt 
      ctctacggc tacaagttc gtctacgag acgtatttg 
      taccacttt actcgcaaa gacacgcgc cacaacttc 
      tcgctttac ttctacctc caatatctc actgcgtgg 
      ataaaaaat atcgggatt tggcaaaaa gttttagtc 
      gtgttacca caacttgtg ctcttgtta gtgttttcg 
      ttccggtac ggtttgaat cgcttctcg ctgaatttc 
      tcagttcta gcccaaact atagtcatg gtgacttac 
      aacagtgtc ttaacttca cagtatttt gtgtggatt 
      atggcggtt ttgccgctg tgtctttgg caaattcgg 
      atgagtgtt aataatgct atttttttg gttgttgtg 
      tggtttgcg gcacaaggg gcttggttg ctcccagcg 
      tattttttg gagtttcac ggccaaaac acctttctt 
      tttatttgg attcaaagt gtgtcattt ttctgcgcc 
      aacattgca attttaggg agacttatc atgtttttt 
      gcgccggta aagaaggaa gtgtaa
    mRNA sequence
    • >TC003602 RA
      gttggttgt gcttcccct attcagtaa gaacgattt 
      caagcattt cttcaacat cttcattct taactctgt 
      cacagccac ttttgctcc tccttctac gcccagggc 
      tcgtgaacc ctttaagtt cgccctttt ctataagct 
      tcatttcct tactgactt gtctataaa ttttttctg 
      atactccag ttgccagta atactcttt gcctgtagt 
      gggcaaggg gcacaacag gagcctgag tttctgctt 
      cttttatca taaatttca acacattgg cgataattg 
      cccgacttt gggcattaa ccactttcc cccgcaccc 
      ccacctgtt tttcaggtt ccatattac aatttttat 
      aaaactaca ttaattttg tatattaaa aaaaatgta 
      aacaataat ggggttatc aattcacta ataccaacc 
      acaagtggc taaaatcca ctacaacga cagtaaatt 
      ttccactag aagactgga aaaagtttt ccgcaatta 
      atactaaac gaagcgttt taccagtcg gttagtttt 
      tattttcgc ccctatgcc caagaaaga cccgtgcaa 
      agtgttcgc gtgcaggat tcagaagtg tctcgaaga 
      taacaaata ccaggagtc tgcctgcca gcacgccat 
      tgaggacct ccacaactg ttgccgcaa gtggacgaa 
      ccagagctt cgtctgcga gggtatcag aatcgaccc 
      agcccccag gaaggagta atgaaacaa aagccgctt 
      tttaaggac agaccgctc atttatcgg tatcctcga 
      acgtgtgct ggacttctg accgccggc ggtctttta 
      gttttcttc tcgcggccg atttatgac ttgtgtttc 
      cgccacttc gatcctgca gagcttgaa gagttgcaa 
      aggacccac aagtcgcga tttgggagg acataatgg 
      aatcagtgc agaaagcgc agcagcggt tccgccagt 
      atcccgtaa ttttagcaa agtgtagta aagaagcaa 
      caaactatg ccgcttgtg tcttgaaga gggacaacg 
      tggggctgg acgactgca aggaggagt tcgggaggt 
      tcaaaacgt gtcttcaga aaacagcgg cgagtttga 
      aaactagac tgtagtttg ttaaaatgg cgtggcgta 
      aattcgtta gtctagatt acaaagtcc atttgcttg 
      tttcccttg ttgttaggc tggctctag tggcctatg 
      gggtctacc atgatcagg tctcctcag tcccttaca 
      cggacatcg actataaag tcttcacag acgcggcac 
      gacacgtcc ttgaccaca aatcgccct ttgaaagac 
      acacttatc gctattcgc cccttgtgg cctatctca 
      tgattccca acatcaccc tccactcca gcttcggca 
      aaatcctgt tctgcatta tcgacttaa tcgacgcca 
      ccttgatcc gaataatag tcaaaacga cgctccacg 
      aatacttca agtacgtgg gggaacccc aaaactgca 
      tcacggggc gcagccgca agaaaacca aaacgaaga 
      aaaacgacc aaactgaca ccactgcga atttaagtc 
      tggtagtct ggctttaca accccctaa ctctagcca 
      ttgccactc gaggaaatt gtgactcga tcgcggttt 
      ttttcgtgt tggcaacgc tgtatttgt tgcaatgtc 
      gcaaacaat attttgcgg ccggattaa tacacggtc 
      tttcgatac atttccgcc tgtacccga tcgtgtaca 
      gcctcacgt tttacatgt atttgagca agttttcat 
      tttactcgc tggaagata ggcgcaaaa gccacttga 
      gggaaatca ccggtgcga gcgacggca gggttgcca 
      cccgcaagc cggagagaa aaacgattt tcaagagga 
      aatatctgc tttatttgg tcccgaatt tcgatcaga 
      taaggctcg tccagggct gtatcttgt cgcttttct 
      ccctgattg gcatatttt attttctct acggctaca 
      agttcgtct acgagacgt atttgtacc actttactc 
      gcaaagaca cgcgccaca acttctcgc tttacttct 
      acctccaat atctcactg cgtggataa aaaatatcg 
      ggatttggc aaaaagttt tagtcgtgt taccacaac 
      ttgtgctct tgttagtgt tttcgttcc ggtacggtt 
      tgaatcgct tctcgctga atttctcag ttctagccc 
      aaactatag tcatggtga cttacaaca gtgtcttaa 
      cttcacagt attttgtgt ggattatgg cggttttgc 
      cgctgtgtc tttggcaaa ttcggatga gtgttaata 
      atgctattt ttttggttg ttgtgtggt ttgcggcac 
      aaggggctt ggttgctcc cagcgtatt ttttggagt 
      ttcacggcc aaaacacct ttcttttta tttggattc 
      aaagtgtgt catttttct gcgccaaca ttgcaattt 
      tagggagac ttatcatgt tttttgcgc cggtaaaga 
      aggaagtgt aaaggaatc gtgctgttt gtagctaag 
      tagttgtaa tgtgtattt gtacagttt ataacagaa 
      taaaataaa ccgaataaa gtagc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY