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Gene information
General information
TC003573 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7858381..7860128
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003573
    iBeetle screen iB_00587

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition 3 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    egg chamber not present - Number of animals showing the phenotype: 5

    mature egg not present - Number of animals showing the phenotype: 5
    vitellarium not present - Number of animals showing the phenotype: 5
    tropharium size shortened - Number of animals showing the phenotype: 5
    tropharium shape irregular - Number of animals showing the phenotype: 5
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003573 RA
      MADSNKPPE TPSETMRKK SESENKPSQ SHDEDNESM 
      EEQPLDIGV HYLVQRSDD SWYPAEIIQ TRYGDSEYE 
      YYVHYEGQN RRLDEWVPR ARIMNSRFN LTESGQKMP 
      TDKVWKERP VVSDLLTDS SDRKITRNQ KRRHDEINH 
      IQKTYAEMD PTTAALEKE HEQITKVKY IDKIQIGRF 
      EIDTWYFSP YPEEYGRQS KLWICEYCL KYMRLEKSY 
      RYHMSECTW RQPVGKEIY RKGTLSVYE VDGKDHKIY 
      CQNLCLLAK LFLDHKTLY FDVEPFLFY ILCEVDKQG 
      AHIVGYFSK EKESPDGNN VACILTLPP YQRQGYGKL 
      LIAFSYELS RLEGTVGSP EQPLSDLGK LSYRSYWSW 
      VLLEILRDF RGTLSIKDL SQMTSITQT DIISTLQSM 
      NMVKYWKGQ HVICVTPKL VEEHIRSSQ YKRPRLCVD 
      STALRWAPR RHTNNKLGK K
    CDS sequence
    • >TC003573 RA
      atggctgat agcaacaag ccaccggag acgccttcc 
      gaaacaatg cgtaaaaaa tcagaaagt gagaacaaa 
      ccgtcgcaa tcacacgat gaagacaac gaaagtatg 
      gaagaacag cctctggac atcggggtg cattatttg 
      gtgcaaaga tcggacgat tcgtggtat cctgccgag 
      ataatccag acgagatat ggtgacagt gagtacgag 
      tattacgtg cattatgag gggcagaat cggcggctg 
      gacgaatgg gtgccaagg gctaggatt atgaattcc 
      aggtttaat ttaacggag agtggacag aaaatgccc 
      actgacaag gtgtggaag gagcggcca gtggtttct 
      gatttattg actgacagt tcggaccgg aaaatcacc 
      aggaatcag aagagaagg cacgatgaa ataaaccac 
      atccagaag acatacgcg gaaatggac ccaactact 
      gcagcttta gagaaggaa catgaacaa ataaccaaa 
      gttaaatat attgataag atacaaatt gggcggttt 
      gagatcgac acatggtat tttagccca tatcctgag 
      gaatatggc cgacagagc aaattatgg atatgtgaa 
      tattgcctc aaatacatg agattagaa aaaagttac 
      agatatcat atgagcgaa tgtacatgg agacaaccg 
      gttggtaaa gaaatttac agaaaagga acactttcg 
      gtttatgaa gttgacgga aaagaccac aaaatttat 
      tgccagaat ctttgcctc ctggcgaag ctgtttcta 
      gaccacaaa acactgtac tttgacgtt gaacctttc 
      ctcttttac attttatgc gaagttgac aagcaaggg 
      gcacacatt gttggttac ttctccaaa gagaaagaa 
      tctccagac ggaaataac gtcgcgtgt atcctcaca 
      ttgccccct tatcagagg caaggctac ggcaagctt 
      ttgatagcc ttcagttac gagttgtcg cggcttgag 
      ggcaccgtg ggcagcccc gaacagccc cttagtgat 
      ttgggaaaa ttgagttat aggagttac tggagttgg 
      gttttgttg gagattttg agggacttt cggggcact 
      ttgtcgatc aaagatttg agccaaatg actagtatt 
      acccagact gatataatt tcaaccttg caaagcatg 
      aatatggtc aaatattgg aaaggtcag cacgtgatt 
      tgtgtcaca cccaaactg gtagaagaa catatcaga 
      tcaagtcag tacaaaagg ccgcgattg tgtgttgac 
      agtacggcg ctgcggtgg gcccccaga agacacacc 
      aacaataag ttaggaaaa aaatga
    mRNA sequence
    • >TC003573 RA
      cgccgccaa aatttcaag atggctgat agcaacaag 
      ccaccggag acgccttcc gaaacaatg cgtaaaaaa 
      tcagaaagt gagaacaaa ccgtcgcaa tcacacgat 
      gaagacaac gaaagtatg gaagaacag cctctggac 
      atcggggtg cattatttg gtgcaaaga tcggacgat 
      tcgtggtat cctgccgag ataatccag acgagatat 
      ggtgacagt gagtacgag tattacgtg cattatgag 
      gggcagaat cggcggctg gacgaatgg gtgccaagg 
      gctaggatt atgaattcc aggtttaat ttaacggag 
      agtggacag aaaatgccc actgacaag gtgtggaag 
      gagcggcca gtggtttct gatttattg actgacagt 
      tcggaccgg aaaatcacc aggaatcag aagagaagg 
      cacgatgaa ataaaccac atccagaag acatacgcg 
      gaaatggac ccaactact gcagcttta gagaaggaa 
      catgaacaa ataaccaaa gttaaatat attgataag 
      atacaaatt gggcggttt gagatcgac acatggtat 
      tttagccca tatcctgag gaatatggc cgacagagc 
      aaattatgg atatgtgaa tattgcctc aaatacatg 
      agattagaa aaaagttac agatatcat atgagcgaa 
      tgtacatgg agacaaccg gttggtaaa gaaatttac 
      agaaaagga acactttcg gtttatgaa gttgacgga 
      aaagaccac aaaatttat tgccagaat ctttgcctc 
      ctggcgaag ctgtttcta gaccacaaa acactgtac 
      tttgacgtt gaacctttc ctcttttac attttatgc 
      gaagttgac aagcaaggg gcacacatt gttggttac 
      ttctccaaa gagaaagaa tctccagac ggaaataac 
      gtcgcgtgt atcctcaca ttgccccct tatcagagg 
      caaggctac ggcaagctt ttgatagcc ttcagttac 
      gagttgtcg cggcttgag ggcaccgtg ggcagcccc 
      gaacagccc cttagtgat ttgggaaaa ttgagttat 
      aggagttac tggagttgg gttttgttg gagattttg 
      agggacttt cggggcact ttgtcgatc aaagatttg 
      agccaaatg actagtatt acccagact gatataatt 
      tcaaccttg caaagcatg aatatggtc aaatattgg 
      aaaggtcag cacgtgatt tgtgtcaca cccaaactg 
      gtagaagaa catatcaga tcaagtcag tacaaaagg 
      ccgcgattg tgtgttgac agtacggcg ctgcggtgg 
      gcccccaga agacacacc aacaataag ttaggaaaa 
      aaatgatgt gataatatg ctttaagtt gtattacct 
      tgagtttgt tgctgaata aagattttt attacca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY