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Gene information
General information
TC003567 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7606719..7609855
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003567
    iBeetle screen iB_00586

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003567 RA
      MVRYAELKG LYEIEKTIG CGGFAKVKL ATHVATGEK 
VAVKIMNKT GLGDDLPRV KLELKALKS FSHQHICKL 
YQVIESETH FFIVIEYCS GGELFDHIV EKNRLSESE 
SRTFFRQIV SGVAYLHSL GYAHRDLKP ENVLLDKDQ 
NLKLIDFGL CARPEGGMG SPLYTSCGS PTYAAPELV 
LGKKYLGPE VDVWAMGVL LYALLVGAL PFDDINIDS 
LYKKILSGK YAEPPFLSA ESKRLIASM LQVDPKKRI 
TVQELLSHP WLTLGILDP VDYTSIDLR KYDKDCVDV 
MASYYNVNP ERMWRHLKK WKYDYHTAT YFLLLSRKK 
RGAVLRLVS LPGQLHLKM RLEETPRKE RPQLKPLVI 
TQGQQLGAK EGQTPYYDC IENIENCKP ARNDNLDSP 
NVFVEPEKP PSVRKPQKR VRSPTLGEE SSPVIYFIA 
LKT
    CDS sequence
    • >TC003567 RA
      atggtgcgt tacgctgaa ctgaaaggc ttgtatgag 
attgagaag acgataggc tgtggagga tttgctaaa 
gttaagctc gcaactcat gtagcaacc ggagaaaag 
gtcgcagtt aaaataatg aacaagacg ggacttggc 
gatgacttg ccaagggtc aaactggaa ctgaaggct 
ttaaagtcg ttctcacac caacacatt tgcaaactc 
taccaagtg attgagtcg gaaacgcac tttttcata 
gttattgag tactgctca ggtggcgaa ctgtttgac 
cacattgtc gaaaaaaac cgcttgagt gaatcggaa 
agccgcact tttttccgg caaattgtg tcaggggtg 
gcttatttg cacagtctg gggtatgca cacagggac 
cttaaacca gagaacgtc cttctcgat aaagaccag 
aacttgaag ctgattgat tttggctta tgtgcccgg 
cctgagggg ggcatgggg agccccctg tacacgtct 
tgcggctct ccaacctac gcggccccc gagctagtc 
ttggggaag aaatatctg gggcccgaa gtggacgtc 
tgggccatg ggggtcctg ctctatgct ctcctagtc 
ggggctttg ccctttgat gacataaac atcgatagc 
ttgtataaa aaaattctg agtggcaaa tacgcggag 
cctcctttc ctgtccgcg gaaagcaag cgcctcatc 
gcctccatg ctccaagtg gaccccaag aaacgcatc 
accgtgcaa gagctcctc tcacaccct tggctcacg 
ttgggtatc ctggaccca gtcgattac acctcaatt 
gatttgaga aagtacgac aaggattgt gtcgacgtt 
atggcatcg tattacaac gtaaacccg gaaagaatg 
tggcgccac ttgaaaaaa tggaaatat gattatcac 
accgctacg tattttttg ttgctttcg aggaaaaaa 
agaggggct gttttgaga ctggtgtca ttgccgggg 
caactacac ttgaaaatg cggttggag gagactccg 
cgtaaagaa aggccccag ttgaaaccc ttggtgata 
actcaagga cagcaatta ggtgcaaaa gagggacag 
acgccgtac tacgactgt atcgagaat atcgaaaat 
tgcaaaccg gcgagaaat gataatctg gatagtccc 
aatgtgttt gttgaacct gagaagccc ccaagcgta 
cgcaaacct cagaagagg gtgaggagc cccactttg 
ggagaggaa agttcgcca gtcatttac ttcattgct 
cttaaaact taa
    mRNA sequence
    • >TC003567 RA
      cagatgtca aaacaattc gaattcaaa tgcggaaaa 
ttcgttaat gtttgcaaa tttctaaca aaaatatgt 
gtgtaattt gagcaaaaa atcgtgcaa ctgtccgtt 
tttgtgtga taaagtagt cggagaaca agtgtttgt 
caagtagtc aaaatggtg cgttacgct gaactgaaa 
ggcttgtat gagattgag aagacgata ggctgtgga 
ggatttgct aaagttaag ctcgcaact catgtagca 
accggagaa aaggtcgca gttaaaata atgaacaag 
acgggactt ggcgatgac ttgccaagg gtcaaactg 
gaactgaag gctttaaag tcgttctca caccaacac 
atttgcaaa ctctaccaa gtgattgag tcggaaacg 
cactttttc atagttatt gagtactgc tcaggtggc 
gaactgttt gaccacatt gtcgaaaaa aaccgcttg 
agtgaatcg gaaagccgc acttttttc cggcaaatt 
gtgtcaggg gtggcttat ttgcacagt ctggggtat 
gcacacagg gaccttaaa ccagagaac gtccttctc 
gataaagac cagaacttg aagctgatt gattttggc 
ttatgtgcc cggcctgag gggggcatg gggagcccc 
ctgtacacg tcttgcggc tctccaacc tacgcggcc 
cccgagcta gtcttgggg aagaaatat ctggggccc 
gaagtggac gtctgggcc atgggggtc ctgctctat 
gctctccta gtcggggct ttgcccttt gatgacata 
aacatcgat agcttgtat aaaaaaatt ctgagtggc 
aaatacgcg gagcctcct ttcctgtcc gcggaaagc 
aagcgcctc atcgcctcc atgctccaa gtggacccc 
aagaaacgc atcaccgtg caagagctc ctctcacac 
ccttggctc acgttgggt atcctggac ccagtcgat 
tacacctca attgatttg agaaagtac gacaaggat 
tgtgtcgac gttatggca tcgtattac aacgtaaac 
ccggaaaga atgtggcgc cacttgaaa aaatggaaa 
tatgattat cacaccgct acgtatttt ttgttgctt 
tcgaggaaa aaaagaggg gctgttttg agactggtg 
tcattgccg gggcaacta cacttgaaa atgcggttg 
gaggagact ccgcgtaaa gaaaggccc cagttgaaa 
cccttggtg ataactcaa ggacagcaa ttaggtgca 
aaagaggga cagacgccg tactacgac tgtatcgag 
aatatcgaa aattgcaaa ccggcgaga aatgataat 
ctggatagt cccaatgtg tttgttgaa cctgagaag 
cccccaagc gtacgcaaa cctcagaag agggtgagg 
agccccact ttgggagag gaaagttcg ccagtcatt 
tacttcatt gctcttaaa acttaaacg tttattaaa 
aaaatcaca tgtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY