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Gene information
General information
TC003555 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 2700798..2704280
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003555
      iBeetle screen iB_00585
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Fertility
      mature egg deposition number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 4
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 88.89% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection


      pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (19 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (19 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      Pupal morphology
      antenna rounded - Number of animals showing the phenotype: 3 (16 dpi)
      Days post injection



      wings complete not present - Number of animals showing the phenotype: 3 (16 dpi)
      Days post injection



      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003555 RA
        MAYNFNKSA KSLKREHDS EAFSSPEKK LKLTRSESP 
        PIFPVEVKE EILSSGEKM KKKWELNFE PVVTVSHKK 
        SKKKNHASD GNISDTHVR SLFQELPFQ EVENHSSDD 
        LTKTGKKRK KKEHNTFEE KYLSDEPFK VKKKREKLL 
        EVSEQHTKK HKKKKEAHG EEMEEEFPT DRMNKESFT 
        DSDIKSYGK ESKKKKKQK RNHNDIGIS SDYSINEDN 
        ANEVEQAKP KKHTKQNNL NNESGSKEQ NIWKKPANG 
        ITETRTDVR SLFEELPFQ EAKKIEIKK MKHNTEEKH 
        FADDETFKV KKNNENLLV VEERKKKHK KKKEVYGEE 
        AEDMENFPT EKIKREFFT DSDSTSYGK EPRKKKKEK 
        TNRDDSESD RLLVEEEHK KKHKKKKEV YGEETEKFA 
        TKKMKGTFT DSDSTSYGK EPKKKKKDK WNRDDSEMS 
        TDYSINDEN LNETEIFTT EPTKHKKHK KKNHSIDEN 
        ASKETEVQK KKKHKHKYQ ELPIQTLSR SFQNNSKDD 
        DKNSRKESE NLQTIPETS ALPKEVENC QKSPKRHKH 
        SELEVSNSG DENSKTKKK PQVLAENMK TENLSIDDL 
        LLHHSVDIK SMDMEYETP EIQHESRKF EESDQINTS 
        VARRSENPQ ESLENVKNE SSDSALDVS EGPENDKCK 
        HKKKFNTDV SEEKFNTRE LCLQKNESV DIDFNDILE 
        DEKNLLTAE ERKRLTDLS VLLPWPVHP LHMVETRIA 
        GRPSKDQWN IIYARSFKL KQGIYSKSE DDIIRRNWE 
        NFCKLHDMP LDPKYFLRL ATPEKEILI PVKERIRFV 
        QFLAHGLPN RLLCSVYKR FQRLFFPKE IITGRFDPR 
        EDKVILDYL KTCKSETPF SDLAKILNR DRLAIEKRS 
        RKLKYINEG ITEVKWTAD KIAEFMQHF VDVTKVDSV 
        DELEDRHIT VEEWKEMSA RMNLPSIKL RPAWFVRIY 
        PRIFIHRSV KMNVVKILL IDVLIEKGK TDWRDVNWE 
        EIAEEFDGF NGQALNKIM KELVRNYVP LSQRSDLGE 
        CLKCLEKEK TRIRGLKMR KIDIIDG
      CDS sequence
      • >TC003555 RA
        atggcatac aatttcaac aaaagtgct aaaagtcta 
        aagcgagag catgattct gaggcattt tcatcacct 
        gagaaaaag ttaaaattg accaggagc gaaagcccc 
        ccgattttt cctgtagag gttaaagag gaaatttta 
        agcagtggt gagaaaatg aaaaagaaa tgggaactc 
        aattttgaa ccagtagtg actgtttca cataaaaaa 
        tccaaaaag aagaatcat gcttctgat gggaatatc 
        tctgatact cacgtgcga agccttttc caagaatta 
        ccgtttcaa gaagtcgaa aaccattca agtgatgac 
        ttaaccaaa actgggaag aaaaggaag aaaaaagaa 
        cataacact tttgaagaa aaatatttg agtgatgaa 
        ccgtttaaa gttaaaaag aaaagggaa aaattgctt 
        gaggtttcg gaacaacat acgaaaaaa cataagaag 
        aaaaaagaa gcgcatggc gaagaaatg gaggaagaa 
        tttcctaca gacagaatg aacaaggaa agttttact 
        gatagtgac atcaaaagt tacggaaag gagtctaaa 
        aagaaaaag aagcaaaaa cggaatcac aatgatatt 
        ggaatatca agtgattat tctatcaat gaagataat 
        gcaaacgaa gtggagcaa gctaaacca aaaaaacat 
        acaaaacaa aataattta aataacgaa agtggatca 
        aaagaacaa aatatttgg aaaaaacca gcaaatggt 
        attacggaa acacgcact gacgtacga agtcttttc 
        gaagaatta ccgtttcaa gaagcaaag aaaattgaa 
        ataaaaaag atgaaacac aacacggaa gaaaaacat 
        tttgctgat gatgaaaca tttaaagtt aaaaagaac 
        aatgaaaac ttgcttgtt gtggaagaa cgtaagaag 
        aaacacaag aagaaaaaa gaagtgtac ggtgaagaa 
        gcggaagat atggaaaat ttccctacg gagaaaata 
        aagagagag ttttttact gatagtgat agcacgagt 
        tatggaaaa gagcctaga aagaaaaag aaggaaaaa 
        acgaaccgc gatgatagt gaaagtgat agattgctt 
        gttgaggaa gaacataag aaaaaacat aagaagaaa 
        aaagaagtg tatggtgaa gaaacggaa aaattcgct 
        acgaagaaa atgaagggg acttttacc gatagtgat 
        agcacaagt tatggaaaa gagcctaaa aagaaaaag 
        aaggacaaa tggaaccgc gatgatagt gaaatgtcg 
        actgattat tccatcaat gatgagaat ttaaacgaa 
        acggaaatt tttacaacg gaaccgact aaacataag 
        aaacataaa aagaaaaat cattcaatt gacgaaaat 
        gcatccaaa gaaaccgaa gttcaaaag aagaagaaa 
        cacaaacat aaataccaa gaattacct attcaaact 
        ttaagtagg tcattccag aataattct aaagacgac 
        gataaaaat tcgagaaag gaatctgaa aatttgcaa 
        acaattccc gaaacatcg gcgctccct aaagaagta 
        gaaaattgt cagaaatcg ccaaaaagg cacaaacat 
        tccgaactt gaagtttct aactcagga gacgaaaat 
        tccaaaacc aaaaagaaa cctcaggta cttgctgaa 
        aatatgaaa actgaaaat ttatcgatt gatgatttg 
        ctgctacat cattctgta gatattaaa agcatggat 
        atggaatac gaaactcca gaaattcaa catgaatcg 
        agaaaattt gaagaaagt gaccaaata aacacttct 
        gtggctcgt aggtcagaa aatcctcaa gaatcgctt 
        gaaaatgtt aaaaatgag tcgtctgat tcggcattg 
        gacgtttct gaaggtcct gaaaacgac aaatgtaaa 
        cacaagaag aaattcaat actgacgtt tctgaggaa 
        aaatttaat acgagagag ttgtgttta caaaaaaac 
        gaatcagtc gatatagat tttaatgac attttggaa 
        gatgaaaaa aatttattg actgcggaa gagcgtaag 
        agattgacg gaccttagc gtgcttcta ccatggcca 
        gttcaccca ttacacatg gtagaaaca cggatagct 
        ggaaggcca tcaaaggat caatggaat ataatttat 
        gccaggagt ttcaaacta aaacaggga atttatagt 
        aaatctgaa gacgacatt attaggaga aattgggaa 
        aacttttgt aaacttcac gatatgcct ttagaccct 
        aaatatttt ctacgttta gctactcca gaaaaggaa 
        attcttatt ccagtcaaa gaaagaata agatttgtc 
        caatttcta gcacacgga ctgcctaat cgcttattg 
        tgttctgta tataagagg tttcagaga ttgtttttc 
        ccaaaagag attataact ggaaggttt gacccaagg 
        gaagataaa gtcattctg gactatttg aaaacatgc 
        aaatcggaa acgccattc tcagatcta gcgaaaatc 
        ctaaatcgg gacagactt gcgattgag aaaaggagc 
        aggaagtta aaatacatc aacgaaggc ataactgag 
        gtcaagtgg acggcggat aaaatagcc gaatttatg 
        caacacttt gttgatgtt acgaaagtc gacagtgtt 
        gacgagctg gaagacagg catatcact gttgaggag 
        tggaaggaa atgtctgct aggatgaat ctaccgagt 
        attaagctg cggcctgcg tggtttgtt agaatatac 
        cctcgcatc ttcatccac cgcagtgtt aaaatgaac 
        gttgttaag atacttttg attgacgtc ttaatcgaa 
        aaggggaaa actgactgg agggatgtc aactgggaa 
        gaaattgct gaagaattt gacggtttc aacggacaa 
        gccttgaat aaaatcatg aaggaactt gttagaaac 
        tacgttcct ttaagtcaa cgaagtgat ttaggagaa 
        tgtttaaag tgcctggag aaggaaaaa acgagaatt 
        cgtggacta aaaatgcga aaaatagat attatagat 
        ggttga
      mRNA sequence
      • >TC003555 RA
        tgctatctg tcaaaaatt tgaaatttc taacctaaa 
        aatttccaa tcacgttgt tgtttagaa ttttcacaa 
        aaatcgtgt aaagcccaa aatggcata caatttcaa 
        caaaagtgc taaaagtct aaagcgaga gcatgattc 
        tgaggcatt ttcatcacc tgagaaaaa gttaaaatt 
        gaccaggag cgaaagccc cccgatttt tcctgtaga 
        ggttaaaga ggaaatttt aagcagtgg tgagaaaat 
        gaaaaagaa atgggaact caattttga accagtagt 
        gactgtttc acataaaaa atccaaaaa gaagaatca 
        tgcttctga tgggaatat ctctgatac tcacgtgcg 
        aagcctttt ccaagaatt accgtttca agaagtcga 
        aaaccattc aagtgatga cttaaccaa aactgggaa 
        gaaaaggaa gaaaaaaga acataacac ttttgaaga 
        aaaatattt gagtgatga accgtttaa agttaaaaa 
        gaaaaggga aaaattgct tgaggtttc ggaacaaca 
        tacgaaaaa acataagaa gaaaaaaga agcgcatgg 
        cgaagaaat ggaggaaga atttcctac agacagaat 
        gaacaagga aagttttac tgatagtga catcaaaag 
        ttacggaaa ggagtctaa aaagaaaaa gaagcaaaa 
        acggaatca caatgatat tggaatatc aagtgatta 
        ttctatcaa tgaagataa tgcaaacga agtggagca 
        agctaaacc aaaaaaaca tacaaaaca aaataattt 
        aaataacga aagtggatc aaaagaaca aaatatttg 
        gaaaaaacc agcaaatgg tattacgga aacacgcac 
        tgacgtacg aagtctttt cgaagaatt accgtttca 
        agaagcaaa gaaaattga aataaaaaa gatgaaaca 
        caacacgga agaaaaaca ttttgctga tgatgaaac 
        atttaaagt taaaaagaa caatgaaaa cttgcttgt 
        tgtggaaga acgtaagaa gaaacacaa gaagaaaaa 
        agaagtgta cggtgaaga agcggaaga tatggaaaa 
        tttccctac ggagaaaat aaagagaga gttttttac 
        tgatagtga tagcacgag ttatggaaa agagcctag 
        aaagaaaaa gaaggaaaa aacgaaccg cgatgatag 
        tgaaagtga tagattgct tgttgagga agaacataa 
        gaaaaaaca taagaagaa aaaagaagt gtatggtga 
        agaaacgga aaaattcgc tacgaagaa aatgaaggg 
        gacttttac cgatagtga tagcacaag ttatggaaa 
        agagcctaa aaagaaaaa gaaggacaa atggaaccg 
        cgatgatag tgaaatgtc gactgatta ttccatcaa 
        tgatgagaa tttaaacga aacggaaat ttttacaac 
        ggaaccgac taaacataa gaaacataa aaagaaaaa 
        tcattcaat tgacgaaaa tgcatccaa agaaaccga 
        agttcaaaa gaagaagaa acacaaaca taaatacca 
        agaattacc tattcaaac tttaagtag gtcattcca 
        gaataattc taaagacga cgataaaaa ttcgagaaa 
        ggaatctga aaatttgca aacaattcc cgaaacatc 
        ggcgctccc taaagaagt agaaaattg tcagaaatc 
        gccaaaaag gcacaaaca ttccgaact tgaagtttc 
        taactcagg agacgaaaa ttccaaaac caaaaagaa 
        acctcaggt acttgctga aaatatgaa aactgaaaa 
        tttatcgat tgatgattt gctgctaca tcattctgt 
        agatattaa aagcatgga tatggaata cgaaactcc 
        agaaattca acatgaatc gagaaaatt tgaagaaag 
        tgaccaaat aaacacttc tgtggctcg taggtcaga 
        aaatcctca agaatcgct tgaaaatgt taaaaatga 
        gtcgtctga ttcggcatt ggacgtttc tgaaggtcc 
        tgaaaacga caaatgtaa acacaagaa gaaattcaa 
        tactgacgt ttctgagga aaaatttaa tacgagaga 
        gttgtgttt acaaaaaaa cgaatcagt cgatataga 
        ttttaatga cattttgga agatgaaaa aaatttatt 
        gactgcgga agagcgtaa gagattgac ggaccttag 
        cgtgcttct accatggcc agttcaccc attacacat 
        ggtagaaac acggatagc tggaaggcc atcaaagga 
        tcaatggaa tataattta tgccaggag tttcaaact 
        aaaacaggg aatttatag taaatctga agacgacat 
        tattaggag aaattggga aaacttttg taaacttca 
        cgatatgcc tttagaccc taaatattt tctacgttt 
        agctactcc agaaaagga aattcttat tccagtcaa 
        agaaagaat aagatttgt ccaatttct agcacacgg 
        actgcctaa tcgcttatt gtgttctgt atataagag 
        gtttcagag attgttttt cccaaaaga gattataac 
        tggaaggtt tgacccaag ggaagataa agtcattct 
        ggactattt gaaaacatg caaatcgga aacgccatt 
        ctcagatct agcgaaaat cctaaatcg ggacagact 
        tgcgattga gaaaaggag caggaagtt aaaatacat 
        caacgaagg cataactga ggtcaagtg gacggcgga 
        taaaatagc cgaatttat gcaacactt tgttgatgt 
        tacgaaagt cgacagtgt tgacgagct ggaagacag 
        gcatatcac tgttgagga gtggaagga aatgtctgc 
        taggatgaa tctaccgag tattaagct gcggcctgc 
        gtggtttgt tagaatata ccctcgcat cttcatcca 
        ccgcagtgt taaaatgaa cgttgttaa gatactttt 
        gattgacgt cttaatcga aaaggggaa aactgactg 
        gagggatgt caactggga agaaattgc tgaagaatt 
        tgacggttt caacggaca agccttgaa taaaatcat 
        gaaggaact tgttagaaa ctacgttcc tttaagtca 
        acgaagtga tttaggaga atgtttaaa gtgcctgga 
        gaaggaaaa aacgagaat tcgtggact aaaaatgcg 
        aaaaataga tattataga tggttgaaa tttgaataa 
        aattaagtt acgtca