Status
Please wait ...
Gene information
General information
TC003553 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 7218623..7234433
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003553
    iBeetle screen iB_00584

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003553 RA
      MGQIPQNTN NLFVDAFLQ SNRLDHVSQ VMGYHTSYL 
      DHFLKTQNF ILRGDGPLP FHYRHYIAI MAAGRHQCG 
      YLINLQKQE FLLLGGDHS WLKGLSSIP PKLKNLYDI 
      NKILAHRPW LLNTSHIEK LTKGKDSWS LAEVIHAIV 
      ILTHFHSLC SFVFACGVN QEVDQAGGF KYQMPGDTR 
      GSAHPSNTN QTNPKDIPT VDITEDWKN SSQVGPPSP 
      TEPEVGINT IMQRMKTLS EQNEECTPV ELAKRFENI 
      ETQSAELGL VGAERLETP ADISCFIED GKFGYQDFA 
      KRDEQSDFP TFRVQDYSW NDHGYSLVN RLYNDVGNL 
      LDDKFKTAQ SLTYYTMGG RTDVDTSRF RRAIWNYIQ 
      CLLGIRHDD YDYGEINQL LERSLKTFI KSACCYPER 
      VTKKDYDRV LREFKHSEK VHVNLMVLE ARMQAELLY 
      ALRTVNRYM TN
    CDS sequence
    • >TC003553 RA
      atgggtcag atcccgcag aatacgaac aatctcttc 
      gtggatgcc tttctacaa agcaacaga ttggaccat 
      gtgagtcag gtaatgggg tatcacacc tcatatttg 
      gaccacttt ctcaagact caaaacttc atactgcga 
      ggagatgga ccacttccg tttcattat aggcattac 
      attgcaata atggccgca ggacgacat cagtgtgga 
      tatttgata aatctccag aagcaagaa tttctcctg 
      ttggggggc gatcacagt tggctcaaa ggccttagt 
      tctatacca ccaaagtta aaaaacctt tacgatatc 
      aataaaata ctagcccat agaccttgg ctgttgaat 
      acgtctcat attgagaaa ttgactaag ggaaaagat 
      agctggtct ttggccgaa gtcatacac gctatagtc 
      atattgact cattttcat tcgctgtgt tcgtttgtg 
      ttcgcttgt ggtgttaac caagaggtg gatcaagcc 
      ggcggattc aagtaccaa atgcccggc gatactaga 
      ggctcggct catcccagc aatacgaat caaactaat 
      cccaaagat ataccaact gtcgatata accgaagac 
      tggaaaaat tcttctcaa gttggtcct ccatcgccc 
      acagaaccc gaagtagga ataaatacc atcatgcaa 
      cgcatgaaa acactctcc gagcagaac gaagaatgc 
      accccagta gaattagcc aaacgtttt gaaaatatt 
      gaaacgcaa tctgccgaa ttaggattg gtaggggcc 
      gaacggtta gaaactcca gcggatatt agttgtttt 
      atcgaagac gggaagttc ggatatcaa gatttcgcc 
      aaaagggac gaacaatcc gacttcccc acgtttagg 
      gtacaggac tattcgtgg aacgaccac ggatactcc 
      ctcgttaat aggttatat aacgacgtg gggaacctg 
      ctggacgac aaattcaag actgcacaa agcctgact 
      tactacacg atggggggc cgcacggac gtggacacg 
      tcgcggttc cgcagggcc atctggaac tacattcag 
      tgtctgctg gggatccgc cacgacgac tacgactac 
      ggggagatc aaccagctc ctggagcgg tcgctgaag 
      acgttcatt aagagcgct tgttgttac ccggagcgc 
      gtgacgaag aaggactac gacagggtt ctcagggag 
      ttcaagcac agcgaaaag gtccacgtg aacctgatg 
      gtcctggag gcgcggatg caggcggaa ctgctgtac 
      gccttgagg actgtgaac cgctacatg acaaattaa
    mRNA sequence
    • >TC003553 RA
      ctgaaatgc agtcagttt cttcacgtc tttagtagg 
      attaaaatc tagttttta aacatctat ggacagaat 
      aatgcagtg acagtcgcc ggaactcct ctccctgtg 
      ataaatcga gtcactttt ccccgaaaa ttaattccg 
      gacgattaa ctcccgttg tgtcctagt gggtgtacc 
      cgaaacaaa aaccaccga ctttgtttt gttttgtgt 
      tgatgacat tcgagtgga gtgtgcccg gagatttca 
      cccccgcgc caagggttt agacgctga ggtcggaca 
      actgccaaa attccggta gccaagcac agcctcgga 
      ccggtccac gaagggaca accatgggt cagatcccg 
      cagaatacg aacaatctc ttcgtggat gcctttcta 
      caaagcaac agattggac catgtgagt caggtaatg 
      gggtatcac acctcatat ttggaccac tttctcaag 
      actcaaaac ttcatactg cgaggagat ggaccactt 
      ccgtttcat tataggcat tacattgca ataatggcc 
      gcaggacga catcagtgt ggatatttg ataaatctc 
      cagaagcaa gaatttctc ctgttgggg ggcgatcac 
      agttggctc aaaggcctt agttctata ccaccaaag 
      ttaaaaaac ctttacgat atcaataaa atactagcc 
      catagacct tggctgttg aatacgtct catattgag 
      aaattgact aagggaaaa gatagctgg tctttggcc 
      gaagtcata cacgctata gtcatattg actcatttt 
      cattcgctg tgttcgttt gtgttcgct tgtggtgtt 
      aaccaagag gtggatcaa gccggcgga ttcaagtac 
      caaatgccc ggcgatact agaggctcg gctcatccc 
      agcaatacg aatcaaact aatcccaaa gatatacca 
      actgtcgat ataaccgaa gactggaaa aattcttct 
      caagttggt cctccatcg cccacagaa cccgaagta 
      ggaataaat accatcatg caacgcatg aaaacactc 
      tccgagcag aacgaagaa tgcacccca gtagaatta 
      gccaaacgt tttgaaaat attgaaacg caatctgcc 
      gaattagga ttggtaggg gccgaacgg ttagaaact 
      ccagcggat attagttgt tttatcgaa gacgggaag 
      ttcggatat caagatttc gccaaaagg gacgaacaa 
      tccgacttc cccacgttt agggtacag gactattcg 
      tggaacgac cacggatac tccctcgtt aataggtta 
      tataacgac gtggggaac ctgctggac gacaaattc 
      aagactgca caaagcctg acttactac acgatgggg 
      ggccgcacg gacgtggac acgtcgcgg ttccgcagg 
      gccatctgg aactacatt cagtgtctg ctggggatc 
      cgccacgac gactacgac tacggggag atcaaccag 
      ctcctggag cggtcgctg aagacgttc attaagagc 
      gcttgttgt tacccggag cgcgtgacg aagaaggac 
      tacgacagg gttctcagg gagttcaag cacagcgaa 
      aaggtccac gtgaacctg atggtcctg gaggcgcgg 
      atgcaggcg gaactgctg tacgccttg aggactgtg 
      aaccgctac atgacaaat taaaggagc gcccccgtg 
      gaaggaaac agtttcagt ccatcggcc catcgagga 
      tgtgggtat ggatttgta tgttattat attgcacag 
      aagctgaga tgacggata ctgtggttt taaatatta 
      attttattg ttgtctgtt tttaaaacc tattttatt 
      gaagattca aatgtgtta tttatgtaa tattaagaa 
      attgatttc atgtggtaa aactgtatt tatcatgat 
      catttttga atataatta atgtggtaa gtatattat 
      caatcgaac aattattca actcgtaat gtgttgata 
      tttttgtat tgacttttt tctactaat ttgcattaa 
      acttcggta aaggtagaa aaaagtatt gtgcaccat 
      acatattaa atatgtgac cacctttgt aaataattt 
      gaatactat atataataa catttatat tgtgtaaat 
      ataaatttt ctgagttat ggaagttat acatcactg 
      ctctcttaa ttttttaat tgtgtttaa catctttgc 
      gttcactta tctgtggct tccgaccag tgtatataa 
      ttattgttt ttaaacgtt aattattgt ccaatattt 
      tatattgta agtatggat ctggtcaag taagttttt 
      atgttgcga tactgttaa ggaggctta taatattct 
      caaattaat ttttataaa tgactgact ggattattt 
      gaatcgagg ttttccgat ttcgcttag tatttgagt 
      aacattgta aggcattat gcagtcatt ttgttgcga 
      caacaaatg attggggaa accatcttt tctttactt 
      gtatcccaa attttcgtg tcttgatct cagtattaa 
      ttataattt tgaattgaa ttcatattt tagtatatt 
      ttcactgcc atattaata gtttatatt aaacagtaa 
      caaacaaat ttcgccaac tgttcatca ccacaaaaa 
      ttcttcacc tgtttgttc gctgcttaa aaatataaa 
      caaaaattt tacccgatt gatgttttt cgtttcgtt 
      gcaaattct gaaaattat aagtatatt tagaaataa 
      tatgatgct gtgatgtta caatttctg tatttattt 
      gaaacaata tcactgttt gttttcatt gctttgtca 
      atttgaccg aatttttgt ttgtacaaa tttgtttgc 
      cgggtagat atgtaataa accgactct gaaagccat 
      aaactatta catacctac ctccacgct tgcaaactt 
      ctgctattt tttatattg taaaagtta atttcaaat 
      tataaatat tatattaaa aaggtgaat tgacgcaga 
      agtacaaaa tatattatg atcggaaga gtgtaaaat 
      acggatttc ataacgtca ttacagtga gagaaaaac 
      aataaaatt caagtattt ttgcacaca ttttacagg 
      aaattaatc acacccaaa attgtattt cagtcaaaa 
      ttttatgaa tggacctta acaaatgcc tgttatgaa 
      atccctcgt tatgtcaca gttacgttg ttgtggggt 
      attctccaa tttgtgata tttatctta atgtgatta 
      ctgtgtaat agattttag attgtttca attgaatac 
      cttcaataa gttttgttg atgtttaac catagacct 
      ttagtgaga tgttttaat ttaattaat ccactttca 
      aataaagta gtttcatgc ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY