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Gene information
General information
TC003539 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6710439..6712009
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003539
    iBeetle screen iB_00582

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003539 RA
      MLIPLFCVM LTYLTVLLG ASVDNNNSK FVPAFSWNY 
INFSWPSAQ TMAEFGYVP GEDVIAGIK IYKNVIYLA 
LPRIKPSSR VTLASIPID ANKENPLLS PYPNWEMNS 
GSTCDAIQN VLSMEIDKD GIMWVLDAR RVDNNTDCP 
PKIILLDLN DGGKIVDSF QVPDDLCPH RGGCFLNDI 
VVDGDFAYV SDTTSSDPG IFVYNRKLK TSWKARDKT 
MFGDPAAVD FTAQGVQNQ QLSHVNGIA LCCGKEERA 
FFYMPQTSL HIFSTSNSV LKNRSVATA SNLHNFITD 
KGTKQGQSG GMICDTEDN IYYGILPLN AVGKWNVQK 
PLETAAIVE QNDEIINWP DSFSIYDNY LYLITNSIS 
KFSKKGINL NDINFRIIK LQTNSKSYV YC
    CDS sequence
    • >TC003539 RA
      atgcttatc cccctgttt tgtgtgatg ttaacttat 
ttgactgtg ttattaggc gcgagtgtt gacaataat 
aatagcaag ttcgtgcct gctttctcg tggaattac 
attaacttt agctggccc tctgcacaa acaatggcc 
gaatttggg tacgtccct ggagaagac gtgatcgcc 
ggtattaaa atttataaa aacgtcatt tacttggct 
cttccgagg ataaaacca agttcaaga gtaacttta 
gcttccatt cctatcgac gcaaataaa gaaaatcca 
cttctgtca ccgtatccc aactgggaa atgaattcg 
ggatcaact tgtgatgcc attcagaat gttttgagc 
atggaaatt gataaagac ggcattatg tgggtttta 
gacgctcga agagtcgac aataatacc gattgtcca 
ccgaaaatc attttatta gatttgaac gacgggggg 
aaaatcgtc gatagcttt caagtgccg gacgatctg 
tgcccccat cgcggcggc tgcttcctc aacgacatc 
gtcgtcgac ggcgacttc gcctacgtt tcggacacg 
acgtcctca gaccccggg atcttcgtc tacaaccga 
aaactgaag acttcctgg aaggccagg gacaagacc 
atgttcggc gaccccgct gccgtcgat tttaccgca 
caaggtgtg caaaaccag cagttgtcg cacgttaat 
ggcatcgcc ttgtgctgc ggcaaggag gagagggcc 
ttcttttac atgccgcaa acgtcattg cacattttc 
tcaacgagt aattccgtt ttgaaaaat cgatcagtg 
gcaactgcg agcaatttg cataatttt atcaccgac 
aaaggcaca aagcaggga caatcagga ggcatgata 
tgtgacacc gaagataat atttactac ggaatttta 
ccgctaaat gctgtcggt aaatggaat gttcaaaag 
ccgctggaa accgcagcg attgttgaa caaaacgac 
gaaattatt aactggccc gacagtttt tccatttat 
gataattat ttgtatttg atcacaaac agtatttcc 
aaattttcg aaaaaaggc atcaattta aacgacatc 
aatttccga ataattaag ctacaaaca aacagcaaa 
agctatgtt tactgttaa
    mRNA sequence
    • >TC003539 RA
      cggtcatca taaatgaat tcaaattgg ccactccgc 
cggtagtta agtgaacgt cgtgttgga tcacttatt 
ataaccatg cttatcccc ctgttttgt gtgatgtta 
acttatttg actgtgtta ttaggcgcg agtgttgac 
aataataat agcaagttc gtgcctgct ttctcgtgg 
aattacatt aactttagc tggccctct gcacaaaca 
atggccgaa tttgggtac gtccctgga gaagacgtg 
atcgccggt attaaaatt tataaaaac gtcatttac 
ttggctctt ccgaggata aaaccaagt tcaagagta 
actttagct tccattcct atcgacgca aataaagaa 
aatccactt ctgtcaccg tatcccaac tgggaaatg 
aattcggga tcaacttgt gatgccatt cagaatgtt 
ttgagcatg gaaattgat aaagacggc attatgtgg 
gttttagac gctcgaaga gtcgacaat aataccgat 
tgtccaccg aaaatcatt ttattagat ttgaacgac 
ggggggaaa atcgtcgat agctttcaa gtgccggac 
gatctgtgc ccccatcgc ggcggctgc ttcctcaac 
gacatcgtc gtcgacggc gacttcgcc tacgtttcg 
gacacgacg tcctcagac cccgggatc ttcgtctac 
aaccgaaaa ctgaagact tcctggaag gccagggac 
aagaccatg ttcggcgac cccgctgcc gtcgatttt 
accgcacaa ggtgtgcaa aaccagcag ttgtcgcac 
gttaatggc atcgccttg tgctgcggc aaggaggag 
agggccttc ttttacatg ccgcaaacg tcattgcac 
attttctca acgagtaat tccgttttg aaaaatcga 
tcagtggca actgcgagc aatttgcat aattttatc 
accgacaaa ggcacaaag cagggacaa tcaggaggc 
atgatatgt gacaccgaa gataatatt tactacgga 
attttaccg ctaaatgct gtcggtaaa tggaatgtt 
caaaagccg ctggaaacc gcagcgatt gttgaacaa 
aacgacgaa attattaac tggcccgac agtttttcc 
atttatgat aattatttg tatttgatc acaaacagt 
atttccaaa ttttcgaaa aaaggcatc aatttaaac 
gacatcaat ttccgaata attaagcta caaacaaac 
agcaaaagc tatgtttac tgttaattg gaatttgct 
ttatatatg atgtgttta ccattatgg gtaaatgca 
agtaatttt ttttaataa aagatgtgt agaagagta 
gggtttttg tgtcctaat tttttgctt tttactaat 
aatgtcaaa aaattgagg gagcacgtt taaatatct 
gaaaaaata attaggacc atttctcga ttgtttgaa 
atacagcaa cagacgcca aaataacca tttgtattt 
ttgtgcgtt tttaatttt agaattagg gtatcctgt 
tattctctt attgtaaat aacgattgt aacataatc 
ttgaataaa tacgttcta caatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY