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Gene information
General information
TC003514 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6157870..6159805
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003514
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00577

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    remarks: later than schedule,
    Pupal morphology
    remarks: later than schedule,
    Adult morphology
    remarks: later than schedule,
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003514 RA
      MIRQEQMFA MKRSPPYRP QQDKISHIV KQPSVINQR 
      VKPKVTIRN QSPLQKATA QAVLNVQNS ETRSKDFSK 
      VPKHRARSV SAVRLGNKE REAWGQILQ SNKSKEKVS 
      LFSNFDPLR TLHFLSKEL FTKLENVFP EEDEMLQMV 
      ADIQHALKR IPPEISTLL HNPLSEASV ATIKNTAAK 
      TCQTPPTLY QEDTEKFQK IMEENTYRL EASCKQLEI 
      VCTNLQNEK ELTEKELDQ ARENVEMLC GRVKELEIE 
      MEEISTMKI KQLEEEKQC LQEQLNKAK QELKDNPTV 
      NHLKKVVED LKKQKATSE ETCARLEYD MALLKMEKE 
      KFVTMLAVR DKEIKEIQE EMTKIQDQV NTQLKKLNG 
      DVLKRANSL KTLKDSSNS PQDLFLEIG DTTISSITS 
      NDSDKEPIN IIKESPCGD AEMNTNGME SQDNFFQKL 
      KADEVEINL PTANDKDQD MGFEDLLQG LTPSVKALL 
      ASHNL
    • >TC003514 RB
      MNHKLRTVS NSETAATCE KYLNKMIRQ EQMFAMKRS 
      PPYRPQQDK ISHIVKQPS VINQRVKPK VTIRNQSPL 
      QKATAQAVL NVQNSETRS KDFSKVPKH RARSVSAVR 
      LGNKEREAW GQILQSNKS KEKVSLFSN FDPLRTLHF 
      LSKELFTKL ENVFPEEDE MLQMVADIQ HALKRIPPE 
      ISTLLHNPL SEASVATIK NTAAKTCQT PPTLYQEDT 
      EKFQKIMEE NTYRLEASC KQLEIVCTN LQNEKELTE 
      KELDQAREN VEMLCGRVK ELEIEMEEI STMKIKQLE 
      EEKQCLQEQ LNKAKQELK DNPTVNHLK KVVEDLKKQ 
      KATSEETCA RLEYDMALL KMEKEKFVT MLAVRDKEI 
      KEIQEEMTK IQDQVNTQL KKLNGDVLK RANSLKTLK 
      DSSNSPQDL FLEIGDTTI SSITSNDSD KEPINIIKE 
      SPCGDAEMN TNGMESQDN FFQKLKADE VEINLPTAN 
      DKDQDMGFE DLLQGLTPS VKALLASHN L
    CDS sequence
    • >TC003514 RA
      atgattcgg caagaacag atgtttgcc atgaaaaga 
      tcaccacct tacagaccc cagcaagac aaaatctca 
      catatcgta aaacaaccc tcagtcatt aatcagcgc 
      gtcaaaccc aaagtaacc attcgcaat caaagccca 
      ttacaaaaa gccaccgct caagccgta ttgaatgta 
      cagaatagt gagacgcgg agcaaagac ttttcaaaa 
      gttcccaaa cacagagcc aggtcggtg tcggccgtt 
      cgattgggc aataaagaa cgagaggct tgggggcag 
      attttgcaa agcaataag agcaaagaa aaagtgtca 
      ctcttcagt aattttgac ccattgagg acgctgcat 
      tttctgtcg aaagaactc tttacgaaa ctcgaaaat 
      gtgttcccc gaagaggat gagatgcta caaatggtt 
      gctgacata caacatgca ctcaaaaga atacctcct 
      gaaatatcc acattgtta cacaaccct ttatcagaa 
      gcctcagtt gccactatt aaaaatacg gcagcaaaa 
      acgtgtcaa acaccacca actttgtat caggaagac 
      actgaaaaa ttccagaaa ataatggaa gaaaacact 
      tacagactt gaagccagt tgcaaacaa ctcgaaatc 
      gtttgcaca aacttacaa aatgagaaa gaattgact 
      gaaaaggaa ttggaccaa gcaagggaa aatgttgaa 
      atgttatgt ggacgcgtc aaagaattg gaaattgaa 
      atggaagaa atctcaacc atgaaaatc aaacaatta 
      gaggaggaa aaacaatgt ttacaagaa cagctcaat 
      aaagctaaa caagaactg aaagacaat cctaccgta 
      aatcatttg aagaaagta gtggaggat ttgaagaag 
      cagaaagca acgtcagaa gagacttgc gccagatta 
      gaatatgat atggctttg cttaaaatg gaaaaggag 
      aagtttgtg actatgctg gctgtgagg gacaaggaa 
      atcaaggaa attcaagaa gaaatgacc aagatacaa 
      gaccaggtt aacacacag ttgaagaaa ttgaacggt 
      gatgtttta aaacgtgca aattcactc aagacattg 
      aaagattca tcaaatagc ccccaggat ctttttctg 
      gaaatcggt gatactaca atctcatct atcacaagc 
      aatgattcc gacaaagaa ccaataaat ataattaaa 
      gaatctccc tgtggtgac gcagaaatg aacacaaat 
      ggaatggag tcgcaagac aatttcttt caaaaatta 
      aaggctgac gaagtggaa atcaatctg ccgaccgct 
      aatgacaaa gatcaagat atggggttt gaggatctc 
      cttcaaggc ttgacgcct tctgttaaa gctttactt 
      gcatctcat aatttgtaa
    • >TC003514 RB
      atgaaccac aagctacga acagtgtcc aacagtgaa 
      acagcagct acatgcgag aaatattta aacaaaatg 
      attcggcaa gaacagatg tttgccatg aaaagatca 
      ccaccttac agaccccag caagacaaa atctcacat 
      atcgtaaaa caaccctca gtcattaat cagcgcgtc 
      aaacccaaa gtaaccatt cgcaatcaa agcccatta 
      caaaaagcc accgctcaa gccgtattg aatgtacag 
      aatagtgag acgcggagc aaagacttt tcaaaagtt 
      cccaaacac agagccagg tcggtgtcg gccgttcga 
      ttgggcaat aaagaacga gaggcttgg gggcagatt 
      ttgcaaagc aataagagc aaagaaaaa gtgtcactc 
      ttcagtaat tttgaccca ttgaggacg ctgcatttt 
      ctgtcgaaa gaactcttt acgaaactc gaaaatgtg 
      ttccccgaa gaggatgag atgctacaa atggttgct 
      gacatacaa catgcactc aaaagaata cctcctgaa 
      atatccaca ttgttacac aacccttta tcagaagcc 
      tcagttgcc actattaaa aatacggca gcaaaaacg 
      tgtcaaaca ccaccaact ttgtatcag gaagacact 
      gaaaaattc cagaaaata atggaagaa aacacttac 
      agacttgaa gccagttgc aaacaactc gaaatcgtt 
      tgcacaaac ttacaaaat gagaaagaa ttgactgaa 
      aaggaattg gaccaagca agggaaaat gttgaaatg 
      ttatgtgga cgcgtcaaa gaattggaa attgaaatg 
      gaagaaatc tcaaccatg aaaatcaaa caattagag 
      gaggaaaaa caatgttta caagaacag ctcaataaa 
      gctaaacaa gaactgaaa gacaatcct accgtaaat 
      catttgaag aaagtagtg gaggatttg aagaagcag 
      aaagcaacg tcagaagag acttgcgcc agattagaa 
      tatgatatg gctttgctt aaaatggaa aaggagaag 
      tttgtgact atgctggct gtgagggac aaggaaatc 
      aaggaaatt caagaagaa atgaccaag atacaagac 
      caggttaac acacagttg aagaaattg aacggtgat 
      gttttaaaa cgtgcaaat tcactcaag acattgaaa 
      gattcatca aatagcccc caggatctt tttctggaa 
      atcggtgat actacaatc tcatctatc acaagcaat 
      gattccgac aaagaacca ataaatata attaaagaa 
      tctccctgt ggtgacgca gaaatgaac acaaatgga 
      atggagtcg caagacaat ttctttcaa aaattaaag 
      gctgacgaa gtggaaatc aatctgccg accgctaat 
      gacaaagat caagatatg gggtttgag gatctcctt 
      caaggcttg acgccttct gttaaagct ttacttgca 
      tctcataat ttgtaa
    mRNA sequence
    • >TC003514 RA
      tccactgta aacaaaatt ctaacctct aacgtcaaa 
      ttttcccaa gcataaatt catgttgtt tgtttggtt 
      ttgtttaaa ttgaagtgt agtgattat ccagaatga 
      accacaagc tacgaacag tgtccaaca gtgaaacag 
      cagctacat gcgagaaat atttaaaca aaatgattc 
      ggcaagaac agatgtttg ccatgaaaa gatcaccac 
      cttacagac cccagcaag acaaaatct cacatatcg 
      taaaacaac cctcagtca ttaatcagc gcgtcaaac 
      ccaaagtaa ccattcgca atcaaagcc cattacaaa 
      aagccaccg ctcaagccg tattgaatg tacagaata 
      gtgagacgc ggagcaaag acttttcaa aagttccca 
      aacacagag ccaggtcgg tgtcggccg ttcgattgg 
      gcaataaag aacgagagg cttgggggc agattttgc 
      aaagcaata agagcaaag aaaaagtgt cactcttca 
      gtaattttg acccattga ggacgctgc attttctgt 
      cgaaagaac tctttacga aactcgaaa atgtgttcc 
      ccgaagagg atgagatgc tacaaatgg ttgctgaca 
      tacaacatg cactcaaaa gaatacctc ctgaaatat 
      ccacattgt tacacaacc ctttatcag aagcctcag 
      ttgccacta ttaaaaata cggcagcaa aaacgtgtc 
      aaacaccac caactttgt atcaggaag acactgaaa 
      aattccaga aaataatgg aagaaaaca cttacagac 
      ttgaagcca gttgcaaac aactcgaaa tcgtttgca 
      caaacttac aaaatgaga aagaattga ctgaaaagg 
      aattggacc aagcaaggg aaaatgttg aaatgttat 
      gtggacgcg tcaaagaat tggaaattg aaatggaag 
      aaatctcaa ccatgaaaa tcaaacaat tagaggagg 
      aaaaacaat gtttacaag aacagctca ataaagcta 
      aacaagaac tgaaagaca atcctaccg taaatcatt 
      tgaagaaag tagtggagg atttgaaga agcagaaag 
      caacgtcag aagagactt gcgccagat tagaatatg 
      atatggctt tgcttaaaa tggaaaagg agaagtttg 
      tgactatgc tggctgtga gggacaagg aaatcaagg 
      aaattcaag aagaaatga ccaagatac aagaccagg 
      ttaacacac agttgaaga aattgaacg gtgatgttt 
      taaaacgtg caaattcac tcaagacat tgaaagatt 
      catcaaata gcccccagg atctttttc tggaaatcg 
      gtgatacta caatctcat ctatcacaa gcaatgatt 
      ccgacaaag aaccaataa atataatta aagaatctc 
      cctgtggtg acgcagaaa tgaacacaa atggaatgg 
      agtcgcaag acaatttct ttcaaaaat taaaggctg 
      acgaagtgg aaatcaatc tgccgaccg ctaatgaca 
      aagatcaag atatggggt ttgaggatc tccttcaag 
      gcttgacgc cttctgtta aagctttac ttgcatctc 
      ataatttgt aaacaccgt tgtaaatca atgagtatt 
      ttgtatatt ttatttttt attaaagct tttgtacat 
      ttctgattc gttctagtg ttaataaaa gattaaata 
      ctat
    • >TC003514 RB
      acaaaattc taacctcta acgtcaaat tttcccaag 
      cataaattc atgttgttt gtttggttt tgtttaaat 
      tgaagtgta gtgattatc cagaatgaa ccacaagct 
      acgaacagt gtccaacag tgaaacagc agctacatg 
      cgagaaata tttaaacaa aatgattcg gcaagaaca 
      gatgtttgc catgaaaag atcaccacc ttacagacc 
      ccagcaaga caaaatctc acatatcgt aaaacaacc 
      ctcagtcat taatcagcg cgtcaaacc caaagtaac 
      cattcgcaa tcaaagccc attacaaaa agccaccgc 
      tcaagccgt attgaatgt acagaatag tgagacgcg 
      gagcaaaga cttttcaaa agttcccaa acacagagc 
      caggtcggt gtcggccgt tcgattggg caataaaga 
      acgagaggc ttgggggca gattttgca aagcaataa 
      gagcaaaga aaaagtgtc actcttcag taattttga 
      cccattgag gacgctgca ttttctgtc gaaagaact 
      ctttacgaa actcgaaaa tgtgttccc cgaagagga 
      tgagatgct acaaatggt tgctgacat acaacatgc 
      actcaaaag aatacctcc tgaaatatc cacattgtt 
      acacaaccc tttatcaga agcctcagt tgccactat 
      taaaaatac ggcagcaaa aacgtgtca aacaccacc 
      aactttgta tcaggaaga cactgaaaa attccagaa 
      aataatgga agaaaacac ttacagact tgaagccag 
      ttgcaaaca actcgaaat cgtttgcac aaacttaca 
      aaatgagaa agaattgac tgaaaagga attggacca 
      agcaaggga aaatgttga aatgttatg tggacgcgt 
      caaagaatt ggaaattga aatggaaga aatctcaac 
      catgaaaat caaacaatt agaggagga aaaacaatg 
      tttacaaga acagctcaa taaagctaa acaagaact 
      gaaagacaa tcctaccgt aaatcattt gaagaaagt 
      agtggagga tttgaagaa gcagaaagc aacgtcaga 
      agagacttg cgccagatt agaatatga tatggcttt 
      gcttaaaat ggaaaagga gaagtttgt gactatgct 
      ggctgtgag ggacaagga aatcaagga aattcaaga 
      agaaatgac caagataca agaccaggt taacacaca 
      gttgaagaa attgaacgg tgatgtttt aaaacgtgc 
      aaattcact caagacatt gaaagattc atcaaatag 
      cccccagga tctttttct ggaaatcgg tgatactac 
      aatctcatc tatcacaag caatgattc cgacaaaga 
      accaataaa tataattaa agaatctcc ctgtggtga 
      cgcagaaat gaacacaaa tggaatgga gtcgcaaga 
      caatttctt tcaaaaatt aaaggctga cgaagtgga 
      aatcaatct gccgaccgc taatgacaa agatcaaga 
      tatggggtt tgaggatct ccttcaagg cttgacgcc 
      ttctgttaa agctttact tgcatctca taatttgta 
      aacaccgtt gtaaatcaa tgagtattt tgtatattt 
      tatttttta ttaaagctt ttgtacatt tctgattcg 
      ttctagtgt taataaaag attaaatac tat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY