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Gene information
General information
TC003499 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 1463693..1467680
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003499
    iBeetle screen iB_00576
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    remarks: later than schedule, hours 24
    Pupal morphology
    remarks: later than schedule, hours 24
    Adult morphology
    remarks: later than schedule, hours 24
    Fertility
    remarks: later than schedule, hours 24
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003499 RA
      MSVFQDNSL NVDNPQSRE EDEELEEQR RRQEELADL 
      LADGIHAFN YDDSTMNSS VTSVEANEP NYQGSLRNK 
      SEFKYEGSG KSFLNDVTD STIEQCYKD ASQQEQLKI 
      LYDVRVREI NRLQKDFEK YKEEKAKEI GVLKNKSTL 
      AEAEIRHLQ ISLSNSESL LVEKNEIIN KLKEDLALK 
      ESNIDNFKK ILEEQNLEI CTYKSMVNE LQLKLADNN 
      PFSTGTRKS NSEELQKAH QDQIISLET LYQEQSKKA 
      QILEKEKTR LEEDLKKLM KSKTEVEEE NNMAIMALS 
      KKLQSAQQQ CKDLFVLGE AIKKESDHF KERMQQMEA 
      NKFDMADVF QTKKMDKET MVVHMEKLK RMLLDKDIE 
      INTLKAKLK FYDSDLSEL FEYRQILSK MYSCEVKPC 
      SDSEHEELI IFMQRQLQS YQQAVEDRN NQIMNLNSV 
      NKELQEKIE EMIQQTRSD IQSLSQKYS LPQLETMSN 
      ELKNAEVTI NSLKQQLLE NEQTHKCVQ EELKRAEED 
      KQKLLAQLD EFRKITDEN DKVKTQIQK ICLKLGLSE 
      TDLLESDVD VHEVNSVKS KYGQVHEQL QHILAHLEA 
      TSPRRTNFG IIHKMKKDV QNFLNSFHV DKLEAKQIR 
      EKLELWDNM LSDFIESFT HGVKNSELK DTVDDLTNA 
      KTMLEGEKF ALDYELRRC TNEIAEMKK EIAALTENR 
      NNLLKESVD NGHTIQKLK DEILNYKKC IKVMEQNKL 
      EIEKQLENT QKQLNDAKQ ETIDLQKTV NGVNEDVVR 
      KKLFKELSD VESGLEQDK RNSDIVTSD VFQKCLQAQ 
      LLKTELLLR DRLQEENSK KMEKIEEEY KKVIQQRSD 
      LYHKEKEKW KIQEANYRK QFAAVLEEC GRKMETLEK 
      ENIELAKRL HILIQECKT YKSSNIAVL DKYNKLATN 
      HKKAFEDLN KRWASLLEA YMYNATKIE KQTNKNVKN 
      VLKKMVLND REIQLIENI YKEKMKKIV AERHSKS
    CDS sequence
    • >TC003499 RA
      atgagtgtc tttcaagac aattcgtta aatgttgat 
      aacccccaa agccgggag gaggatgaa gagctcgaa 
      gaacagcga cgacgtcaa gaagaatta gctgattta 
      ttagcagat gggattcac gcttttaat tatgacgac 
      agtacgatg aattcatcg gtaacatca gtagaagca 
      aatgagcca aattatcaa gggtcatta cggaacaaa 
      agtgaattt aagtacgaa ggaagtgga aaaagtttt 
      ttaaacgat gttacggac tcaactatt gaacagtgt 
      tataaggac gccagtcag caggagcag ctgaagatt 
      ttatacgat gttagagtc agggaaatt aatcgcctt 
      caaaaagat ttcgaaaaa tacaaggaa gagaaagca 
      aaggaaatt ggagtacta aaaaacaaa tcgactctt 
      gcagaggcg gaaatccgt cacttacaa atctcgcta 
      agcaattct gaaagctta ttagttgaa aaaaacgaa 
      attatcaac aagttaaag gaagatttg gccttgaaa 
      gagtcgaat atcgacaac tttaaaaaa attctagaa 
      gaacaaaac ctagaaata tgcacatac aagtctatg 
      gtcaacgag ttgcaattg aagttggct gataataac 
      ccctttagt acagggaca cgcaaatca aattctgag 
      gaattacaa aaagctcac caggatcag atcatcagt 
      ctggaaact ttgtatcag gaacaatca aaaaaagct 
      caaattttg gaaaaggag aaaacacgt ttggaggaa 
      gatttgaaa aagttaatg aagagcaaa acagaagtg 
      gaggaggaa aataacatg gccattatg gcgttatcc 
      aaaaaactg caaagtgcg caacaacaa tgtaaagat 
      ttgtttgtc ctgggcgaa gctataaaa aaagaaagt 
      gatcatttt aaagagaga atgcaacaa atggaagcc 
      aataaattt gatatggcg gatgtcttt caaaccaaa 
      aaaatggac aaagaaaca atggttgtt catatggaa 
      aaattaaaa cgaatgttg ttagacaaa gacattgaa 
      ataaatacg ctgaaagct aaattgaaa ttttatgac 
      tctgaccta agcgaatta tttgaatac aggcagatc 
      ttaagcaaa atgtattca tgtgaggtt aaaccatgc 
      agtgacagt gaacatgaa gaactgata attttcatg 
      caaaggcaa ctacagagt taccaacaa gctgttgaa 
      gatcgtaat aatcaaata atgaacctg aattcggta 
      aacaaggaa ctgcaggaa aagattgag gaaatgata 
      cagcaaaca agaagtgac attcaaagc ttgtcgcag 
      aaatacagc ctgcctcag ctggaaaca atgagcaac 
      gaattaaaa aatgcagaa gttacaatc aacagttta 
      aaacaacaa ttactggaa aatgagcaa acccataaa 
      tgtgtacaa gaagagttg aagagagcc gaagaagat 
      aaacaaaaa ctgttggct cagttagat gagttcagg 
      aaaataact gacgagaac gacaaagtc aagacccag 
      attcagaaa atttgtctc aagctggga ctttccgaa 
      accgattta ctcgagtct gacgttgat gttcacgaa 
      gttaattca gtaaagtcc aaatatggg caagttcac 
      gaacagttg cagcacatt cttgcccat ctggaagcc 
      acaagtccc agaaggaca aattttgga attattcac 
      aagatgaag aaagacgtg caaaatttt ctcaattcg 
      ttccacgtc gataaactc gaagctaaa caaatccga 
      gaaaaactg gaactttgg gacaacatg ttgagcgat 
      tttattgag agtttcaca cacggtgtg aaaaattcc 
      gaattgaaa gacactgtt gatgacctt acaaacgcc 
      aaaactatg ttagagggt gaaaaattt gccctagac 
      tatgagctg aggcgctgc actaatgaa attgccgaa 
      atgaaaaag gaaattgct gctttgact gaaaacagg 
      aacaatttg ttgaaagaa tcggtagat aatgggcac 
      actattcaa aaattgaaa gacgaaatt ctcaattat 
      aaaaaatgt attaaagtg atggaacaa aataaatta 
      gagattgaa aaacaactg gaaaacact cagaagcag 
      ctgaatgat gcaaaacag gagaccata gacttgcaa 
      aagacagtc aatggagta aatgaagat gtggtgcgg 
      aaaaagtta ttcaaagag ttgtcagac gttgaaagt 
      ggtttggaa caagataaa agaaacagc gatattgtg 
      accagcgac gtgtttcaa aaatgtttg caagcacag 
      ctcttgaaa accgaattg ttgttgcgt gatcggttg 
      caagaagaa aattcaaag aaaatggaa aaaatcgaa 
      gaggaatac aagaaagtc attcaacag cggtccgat 
      ttgtatcac aaggagaag gaaaagtgg aagattcag 
      gaagcaaat tatcgcaaa cagtttgct gcagttttg 
      gaggaatgt gggcgtaag atggaaact ttagaaaag 
      gaaaacatt gagttagcc aagcgcttg cacatcctc 
      atccaggag tgcaaaacg tataaaagt agcaacatc 
      gcggttctg gacaagtat aacaaactg gccacgaac 
      cataaaaaa gcttttgag gatttaaac aagcgatgg 
      gcgtccctt ttggaagcg tatatgtat aatgcaaca 
      aaaattgaa aagcagacc aacaagaac gtgaaaaat 
      gtcctgaaa aaaatggtt ttaaatgac cgagagatt 
      caattaata gaaaatatt tataaggaa aagatgaag 
      aaaatagtt gctgagagg catagcaaa tcctga
    mRNA sequence
    • >TC003499 RA
      tcggaaaac ttttataat ttaacgtta agtaggtga 
      aaatgagtg tctttcaag acaattcgt taaatgttg 
      ataaccccc aaagccggg aggaggatg aagagctcg 
      aagaacagc gacgacgtc aagaagaat tagctgatt 
      tattagcag atgggattc acgctttta attatgacg 
      acagtacga tgaattcat cggtaacat cagtagaag 
      caaatgagc caaattatc aagggtcat tacggaaca 
      aaagtgaat ttaagtacg aaggaagtg gaaaaagtt 
      ttttaaacg atgttacgg actcaacta ttgaacagt 
      gttataagg acgccagtc agcaggagc agctgaaga 
      ttttatacg atgttagag tcagggaaa ttaatcgcc 
      ttcaaaaag atttcgaaa aatacaagg aagagaaag 
      caaaggaaa ttggagtac taaaaaaca aatcgactc 
      ttgcagagg cggaaatcc gtcacttac aaatctcgc 
      taagcaatt ctgaaagct tattagttg aaaaaaacg 
      aaattatca acaagttaa aggaagatt tggccttga 
      aagagtcga atatcgaca actttaaaa aaattctag 
      aagaacaaa acctagaaa tatgcacat acaagtcta 
      tggtcaacg agttgcaat tgaagttgg ctgataata 
      accccttta gtacaggga cacgcaaat caaattctg 
      aggaattac aaaaagctc accaggatc agatcatca 
      gtctggaaa ctttgtatc aggaacaat caaaaaaag 
      ctcaaattt tggaaaagg agaaaacac gtttggagg 
      aagatttga aaaagttaa tgaagagca aaacagaag 
      tggaggagg aaaataaca tggccatta tggcgttat 
      ccaaaaaac tgcaaagtg cgcaacaac aatgtaaag 
      atttgtttg tcctgggcg aagctataa aaaaagaaa 
      gtgatcatt ttaaagaga gaatgcaac aaatggaag 
      ccaataaat ttgatatgg cggatgtct ttcaaacca 
      aaaaaatgg acaaagaaa caatggttg ttcatatgg 
      aaaaattaa aacgaatgt tgttagaca aagacattg 
      aaataaata cgctgaaag ctaaattga aattttatg 
      actctgacc taagcgaat tatttgaat acaggcaga 
      tcttaagca aaatgtatt catgtgagg ttaaaccat 
      gcagtgaca gtgaacatg aagaactga taattttca 
      tgcaaaggc aactacaga gttaccaac aagctgttg 
      aagatcgta ataatcaaa taatgaacc tgaattcgg 
      taaacaagg aactgcagg aaaagattg aggaaatga 
      tacagcaaa caagaagtg acattcaaa gcttgtcgc 
      agaaataca gcctgcctc agctggaaa caatgagca 
      acgaattaa aaaatgcag aagttacaa tcaacagtt 
      taaaacaac aattactgg aaaatgagc aaacccata 
      aatgtgtac aagaagagt tgaagagag ccgaagaag 
      ataaacaaa aactgttgg ctcagttag atgagttca 
      ggaaaataa ctgacgaga acgacaaag tcaagaccc 
      agattcaga aaatttgtc tcaagctgg gactttccg 
      aaaccgatt tactcgagt ctgacgttg atgttcacg 
      aagttaatt cagtaaagt ccaaatatg ggcaagttc 
      acgaacagt tgcagcaca ttcttgccc atctggaag 
      ccacaagtc ccagaagga caaattttg gaattattc 
      acaagatga agaaagacg tgcaaaatt ttctcaatt 
      cgttccacg tcgataaac tcgaagcta aacaaatcc 
      gagaaaaac tggaacttt gggacaaca tgttgagcg 
      attttattg agagtttca cacacggtg tgaaaaatt 
      ccgaattga aagacactg ttgatgacc ttacaaacg 
      ccaaaacta tgttagagg gtgaaaaat ttgccctag 
      actatgagc tgaggcgct gcactaatg aaattgccg 
      aaatgaaaa aggaaattg ctgctttga ctgaaaaca 
      ggaacaatt tgttgaaag aatcggtag ataatgggc 
      acactattc aaaaattga aagacgaaa ttctcaatt 
      ataaaaaat gtattaaag tgatggaac aaaataaat 
      tagagattg aaaaacaac tggaaaaca ctcagaagc 
      agctgaatg atgcaaaac aggagacca tagacttgc 
      aaaagacag tcaatggag taaatgaag atgtggtgc 
      ggaaaaagt tattcaaag agttgtcag acgttgaaa 
      gtggtttgg aacaagata aaagaaaca gcgatattg 
      tgaccagcg acgtgtttc aaaaatgtt tgcaagcac 
      agctcttga aaaccgaat tgttgttgc gtgatcggt 
      tgcaagaag aaaattcaa agaaaatgg aaaaaatcg 
      aagaggaat acaagaaag tcattcaac agcggtccg 
      atttgtatc acaaggaga aggaaaagt ggaagattc 
      aggaagcaa attatcgca aacagtttg ctgcagttt 
      tggaggaat gtgggcgta agatggaaa ctttagaaa 
      aggaaaaca ttgagttag ccaagcgct tgcacatcc 
      tcatccagg agtgcaaaa cgtataaaa gtagcaaca 
      tcgcggttc tggacaagt ataacaaac tggccacga 
      accataaaa aagcttttg aggatttaa acaagcgat 
      gggcgtccc ttttggaag cgtatatgt ataatgcaa 
      caaaaattg aaaagcaga ccaacaaga acgtgaaaa 
      atgtcctga aaaaaatgg ttttaaatg accgagaga 
      ttcaattaa tagaaaata tttataagg aaaagatga 
      agaaaatag ttgctgaga ggcatagca aatcctgaa 
      agcttcatc ttcattttc aatattcta catgtttta 
      attacaatt tgtattttg aacagttaa gaaataaag 
      aaatatatc tttgccagt tcgttttat ttttatctg 
      tcctttatt cacactgag tgataagat aacatgcga 
      taagtaaat ggagataac gtttttatt ttgaattcg 
      ggtgaaaag agcgggtag aacttaccg ttcatgacg 
      tgaggtgcc gctgcagtt tcaaattcg gatcattag 
      ttttcttcg gctttcgaa gcacatgtg aaaagttga 
      cgttttcct gccattttc gtgcgtgtt ccgggtggt 
      gtagttttt ttttgcgcg tcttttctg gaaaaataa 
      tcaaaatac acgtaacta taacca